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Life Science
Published by Universitas Negeri Semarang
ISSN : 22526277     EISSN : 25285009     DOI : https://doi.org/10.15294/lifesci
Core Subject : Agriculture,
Agriculture, Biological Sciences & Forestry
Life Science publishes original and significant articles on all aspects of Life Sciences (Biology, Genetics, Biological Anthropology, Botany, Medical Sciences, Veterinary Sciences, Biochemical Genetics, Biometry, Clinical Genetics, Cytogenetics, Genetic Epidemiology, Genetic Testing, Evolution and Population Genetics, Immunogenetics and Molecular Genetics). The journal also covers ethical issues. It aims to serve as a forum for life scientists and health professionals.
Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Pertanian dan Biologi (Lain-Lain)
Articles 219 Documents
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Keragaman Teripang asal Pulau Pramuka, Kepulauan Seribu Teluk Jakarta
Life Science Vol 1 No 2 (2012)
Publisher : Universitas Negeri Semarang

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Abstract

Teripang (Holothuroidea) atau yang lebih dikenal dengan timun laut merupakan hewan dari kelas Echinodermata. Teripang mempunyai nilai ekonomi yang penting karena kandungan atau kadar nutrisi yang tinggi. Penelitian ini bertujuan untuk dan mengidentifikasi teripang yang ditemukan pada perairan di sekitar P. Pramuka Kepulauan SeribuTeluk Jakarta berdasarkan morfologi dan anatominya. Sampel yang diperoleh dalam penelitian ini berjumlah 13 ekor teripang. Hasil identifikasimorfologi berdasarkan bentuk, corak dan warna serta tipe spikula menunjukkan bahwa teripang pada Pulau Pramuka berasal dari tiga famili, yaitu  Holothuriidae (genus Actinopyga dan Holothuria), Stichopodidae (genus Stichopus), dan Synaptidae (genus Synapta). Jenis teripang yang berhasil diidentifikasi tersebut meliputi Actinopyga lecanora , Holothuria sp , H. leucospilota , H. impatiens , H. fuscocinerea , Stichopus ocellatus , S. quadrifasciatus , S. vastus , dan  Synapta maculata.Sea cucumbers (Holothuroidea) as we known as the sea cucumber is an animal of the class Echinodermata. Sea cucumbers have an important economic value for the content or high levels of nutrients. This research aims to identify sea cucumber that found in the waters around Pramuka Island, Kepulauan Seribu, Jakarta Baybase on the morphology and anatomy.Samples been obtained in this study as much as 13.  The results of morphological identification based on the shape, style and color as well as the type of spicules explained that sea cucumbers at Pulau Pramuka from three families,namely Holothuriidae (genus Actinopyga and Holothuria), Stichopodidae (genus Stichopus), and Synaptidae (genus Synapta).  Types of sea cucumbers were identified include Actinopyga lecanora, Holothuria sp, H. leucospilota, H. impatiens, H. fuscocinerea, Stichopus ocellatus, S. quadrifasciatus, S. vastus, and Synapta maculataand.
Studi Awal Populasi dan Distribusi Macaca fascicularis Ulolanang Raffles di Cagar Alam
Life Science Vol 1 No 2 (2012)
Publisher : Universitas Negeri Semarang

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Abstract

Pembukaan ladang di sekitar Cagar Alam (CA) Uloalanang Kecubung telah mempengaruhi populasi monyet ekor panjang (Macaca fascicularis Raffles, 1821) yang hidup di dalamnya. Monyet ekor panjang dianggap sebagai hama karena sering menyerang ladang,  hal tersebut meningkatkan aktivitas perburuan monyet ekor panjang yang dikhawatirkan menyebabkan menurunnya populasi monyet ekor panjang di CA Ulolanang Kecubung di masa mendatang. Tujuan penelitian ini mengetahui ukuran populasi dan distribusi monyet ekor panjang di CA Ulolanang Kecubung sebagai langkah awal upaya pelestarian monyet ekor panjang di CA Ulolanang kecubung. Penelitian ini menggunakan metode garis transek. Penentuan garis transek mengikuti jalur yang ada yaitu jalur sekat bakar. Lebar areal pengamatan 50 meter ke kanan dan 25 meter ke kiri menyesuaikan kondisi lapangan serta panjang 3 km.  Hasil pengamatan diperoleh populasi monyet ekor panjang berisar antara 30 sampai 36 ekor dengan kerapatan populasi 0,47 ekor/ha (0 sampai 1 ekor/ha) dan mengelompok di daerah  pal 31 dan 51 karena berdekatan dengan sungai (riparian) dan sumber makanan (ladang).The opening of the fields around the Uloalanang Kecubung Nature Reserve has affected populations of long-tailed macaque (Macaca fascicularis Raffles, 1821) live in it. Long-tailed monkeys are seen as pests as they often attack the field, so that hunting activity increases the fear causes decreased long-tailed macaque population in Uloalanang Kecubung Nature Reserve in the future. The purpose of this study was the determination of the population size and distribution of long-tailed macaque in Uloalanang Kecubung Nature Reserve conservation as a first step in the long-tailed macaque Uloalanang Kecubung Nature Reserve. This study used the transect line. Determination of the transect line to follow the path that is the path firebreaks. The width of the observation area of ​​50 meters to 25 meters to the right and left to adjust the condition of the field, and a length of 3 km. Observations obtained berisar long-tailed monkey population between 30 to 36 tail with a population density of 0.47 fish / ha (0 to 1 fish / ha) and clustered in the pal 31 and 51 because adjacent to the river (riparian) and food sources (fields).
Pengaruh Pengasinan terhadap Kandungan Zat Gizi Telur Bebek yang Diberi Limbah Udang
Life Science Vol 1 No 2 (2012)
Publisher : Universitas Negeri Semarang

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Abstract

Tujuan penelitian ini untuk mengetahui pengaruh pengasinan terhadap kandungan gizi telur bebek yang diberi limbah udang. Penelitian ini merupakan penelitian eksperimental Rancangan Acak Lengkap terdiri dari 6 perlakuan yaitu, perlakuan 1 (mentah TLU), perlakuan 2 (rebus TLU), perlakuan 3 (mentah LU) perlakuan 4 (rebus LU), perlakuan 5 (mentah asin LU), dan perlakuan 6 (rebus asin LU). Data yang diperoleh dianalisis secara kuantitatif menggunakan statistik Multvariat Analysis of Variance (MANOVA) pada taraf signifikansi 95% (p = 0,05). Hasil penelitian menunjukkan bahwa penambahan limbah udang dan pengasinan mampu menurunkan kadar air melalui proses pemanasan saat perebusan, meningkatkan kadar abu, meningkatkan lemak, meningkatkan protein, dan menurunkan karbohidrat. Berdasarkan penelitian yang dilakukan dapat disimpulan bahwa, proses penambahan limbah udang dan pengasinan berpengaruh terhadap penurunan kadar air telur bebek mentah, peningkatan kadar abu telur bebek rebus asin, peningkatan lemak telur bebek rebus, peningkatan protein telur bebek mentah asin dan penurunan karbohidrat telur bebek rebus asin. The purpose of this study to determine the effect of marinating duck egg contains nutrients fed shrimp waste. This study was an experimental research with completely randomized design (CRD) consists of 6 treatment, treatment 1 (raw TLU), treatment 2 (boiled TLU), treatment 3 (raw LU) treatment 4 (boiled LU), treatment 5 (raw salty LU), and treatment 6 (boiled salted LU). The data were analyzed quantitatively using statistical Multvariat Analysis of Variance (MANOVA) at 95% significance level (p = 0.05). The results that addition of shrimp waste feed and marinating can reduce water content by heating boiling, increasing ash content, increase fat, increase protein and lower in carbohydrates. Based on the results of research conducted that, the process of adding shrimp waste and salting effect on the decrease in water content of raw duck eggs, elevated levels of ash in boiled salted duck eggs, increased fat poached duck egg, increasing protein in raw salted duck egg and a decrease carbohydrate in boiled salted duck egg. 
Efikasi Ekstrak Daun Lengkuas Terhadap Mortalitas Larva Nyamuk Anopheles aconitus
Life Science Vol 1 No 1 (2012)
Publisher : Universitas Negeri Semarang

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Abstract

Malaria ditularkan melalui gigitan nyamuk Anopheles betina. Penggunaan bahan kimia secara kumulatif berdampak negatif terhadap manusia dan lingkungan, diperlukan upaya untuk mendapatkan alternatif yang aman untuk insektisida. Berdasarkan pertimbangan ini, para ahli menggunakan insektisida nabati. Salah satu tanaman potensial adalah daun lengkuas (Alpinia galanga SW.). Daun lengkuas memiliki bahan aktif berupa tanin, saponin, alkaloid, terpenoid dan flavonoid yang dapat digunakan untuk mengendalikan serangga. Penelitian ini bertujuan untuk mengetahui pengaruh ekstrak daun lengkuas terhadap kematian larva nyamuk, efektivitas (LC50-24 jam dan LC90-24 jam) ekstrak daun lengkuas terhadap kematian larva nyamuk dan mortalitas larva nyamuk pada berbagai tingkat konsentrasi ekstrak daun lengkuas. Metode analisis data yang digunakan ada 3 (tiga), yaitu Uji One Way Anava dengan taraf kepercayaan 95%, analisis regresi dan analisis probit. Hasil perhitungan dengan menggunakan nilai yang diperoleh dengan analisis probit LC50-24 jam dan LC90-24 jam ekstrak daun dari lengkuas adalah 4,4% dan 5,1%. Pengujian Anava satu arah menunjukkan bahwa antar kelompok rata lengkuas daun data konsentrasi ekstrak satu sama lain tidak saling identik atau berbeda secara signifikan. Malaria is transmitted through the bite of female Anopheles mosquito. Use of chemicals cumulatively have a negative impact on humans and the environment, required an effort to get a safe alternative to insecticides. Based on these considerations, the experts use plant-based insecticides. One of the potential plant is the leaf galangal (Alpinia galanga SW.). Leaf galangal has an active ingredient in the form of tannin, saponin, alkaloid, terpenoid and flavonoid that can be used to control insects. The study aims to determine the effect of leaf extract of galangal against mosquito larvae mortality, the efficacy (LC50-24 hours and LC90-24 hours) galangal leaf extract against mosquito larvae mortality and mortality of mosquito larvae to know the difference A. aconitus at various levels of concentration of leaf extract of galangal. Data analysis methods used there are 3, namely Test Anava One Way with 95% confidence level, regression analysis and probit analysis. From the results of calculations using values ​​obtained by probit analysis LC50-24 hour and LC90-24 hours a leaf extract from galangal is 4,4% and 5,1%. In one direction Anava testing showed that the average inter-group galangal leaf extract concentration data with each other are not mutually identical or significantly different.
Efektivitas Ekstrak Tanin Seledri Terhadap Profil Lipid Tikus Putih Hiperkolesterolemi
Life Science Vol 1 No 2 (2012)
Publisher : Universitas Negeri Semarang

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Abstract

Tujuan penelitian ini adalah  untuk mengetahui pengaruh ekstrak tanin  seledri terhadap profil lipid tikus putih hiperkolestrolemi. Ekstrak tanin seledri dilakukan dengan cara maserasi menggunakan pelarut aseton: air (7:3) selama 3X24 jam, kemudian dilakukan dengan fraksinasi. Uji fitokimia dilakukan dengan menambahkan larutan FeCl3 1%. Pemisahan ekstrak tanin seledri dilakukan dengan kromatografi lapis tipis (KLT). Penelitian ini merupakan penelitian experimental dengan rancangan Post Randomized Controlled Group Design pada 20 ekor tikus putih jantan yang dibagi menjadi 4 kelompok masing-masing kelompok terdiri dari 5 ekor. Kelompok I diberikan akuades sebagai kontrol, kelompok II, III dan IV diberi ekstrak tanin seledri dosis 25, 50, dan 75 mg/kg BB/hari selama 14 hari. Data yang telah diperoleh dianalisis dengan statistika metode Anova satu arah dengan taraf kepercayaan 95%. Dari hasil penelitian menunjukkan bahwa adanya efek signifikan menurunkan kadar kolesterol total dan LDL. Akan tetapi ekstrak tanin seledri tidak berpengaruh siginifikan terhadap kadar trigliserida dan HDL.The purpose of this research was to determine the effect of tannins extract of celery on lipid profile hiperkolestrolemi white rats. Tannins extract of celery  is done by maceration using the solvent acetone: water (7:3) for 3x24 hours. This research with the scheme would Randomized Controlled Group Design Post on 20 male white rats were divided into 4 groups each group consisting of 5 animals. Group I given distilled water as control, group II, III and IV was given tannins exract of celery dose of 25, 50 and 75 mg / kg bw / day for 14 days. The date have been obtained were analyzed with one-way ANOVA statistical method with 95% confidence level. From the experimental results, it is known that the three doses used tannin extract of celery in 14 day significantly decreasing the cholesterol total and LDL. But tannins extract of celery doesn’t show effect significantly however HDL and triglicerides.
Pengaruh Suhu dan Waktu Penyimpanan Terhadap Pertumbuhan Bakteri dan Fungi Ikan Bandeng
Life Science Vol 1 No 2 (2012)
Publisher : Universitas Negeri Semarang

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Abstract

Tujuan penelitian ini mengetahui pengaruh suhu dan waktu penyimpanan terhadap pertumbuhan bakteri dan fungi pada ikan bandeng (Chanos chanos). Variasi suhu penyimpanan adalah -6°C, 10°C, dan 30°C, sedangkan variasi lama waktu penyimpanan  adalah 24 jam, 48 jam dan 72 jam. Sebanyak 9 ekor ikan bandeng dimasukkan ke dalam 3 plastik, masing-masing plastik/kelompok terdiri dari 3 ekor. Kelompok 1 disimpan dalam freezer (suhu beku -6oC), kelompok 2 disimpan di kulkas (suhu dingin 10oC) dan kelompok 3 di ruangan (suhu kamar 30oC). Masing-masing kelompok suhu disimpan selama 24 jam, 48 jam, dan 72 jam. Jumlah pertumbuhan bakteri dan fungi dalam media tanam PDA dianalisa secara deskriptif. Hasil penelitian menunjukkan bahwa suhu dan waktu penyimpanan berpengaruh terhadap pertumbuhan bakteri dan fungi pada ikan bandeng. Suhu beku (-60C) dan suhu dingin (100C) penyimpanan selama 24 jam dapat menghambat pertumbuhan bakteri dan fungi. Pertumbuhan optimal bagi bakteri dan fungi terjadi pada penyimpanan dalam suhu kamar (300C) selama 24 jam, 48 jam, 72 jam. Simpulan dari penelitian ini adalah. Disimpulkan bahwa suhu dan waktu penyimpanan berpengaruh terhadap pertumbuhan bakteri dan fungi pada ikan Bandeng.The purpose of this study was to know the effect of temperature and storage time on the growth of bacteria and fungi on milkfish (Chanos chanos). Variations storage temperature is    -6°C, 10°C, and 30°C, while the variation of the storage time was 24 hours, 48 hours and 72 hours. A total of 9 milkfish put into 3 plastic, each plastic/group consisted of 3 milkfish. Group 1 was stored in a freezer (freezing temperatures -6oC), group 2 was stored in the refrigerator (10°C colder temperatures) and group 3 in the room (room temperature 30oC). Each group recorded temperature for 24 hours, 48 hours, and 72 hours. Total growth of bacteria and fungi in the growing media PDA analyzed descriptively. The results showed that temperature and storage time effect on the growth of bacteria and fungi on milkfish. Freezing temperatures (-6oC) and cold temperature (10oC) storage for 24 hours to inhibit the growth of bacteria and fungi. Optimal for growth of bacteria and fungi occur on storage at room temperature (30oC) for 24 hours, 48 hours, 72 hours. It was concluded that the temperature and storage time effect on the growth of bacteria and fungi on Milkfish. 
Pengaruh Ekstrak Lengkuas pada Perendaman Ikan Bandeng terhadap Jumlah Bakteri Pengaruh Ekstrak Lengkuas pada Perendaman Ikan Bandeng terhadap Jumlah Bakteri
Life Science Vol 1 No 2 (2012)
Publisher : Universitas Negeri Semarang

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Abstract

Penelitian ini bertujuan untuk mengetahui pengaruh ekstrak lengkuas pada perendaman ikan bandeng terhadap jumlah bakteri dan tingkat penerimaan konsumen. Penelitian ini menggunakan 27 ekor ikan bandeng yang dikelompokkan menjadi 3 kelompok, masing-masing terdiri dari 9 ekor. Ketiga kelompok tersebut berturut-turut direndam dalam larutan ekstrak lengkuas konsentrasi 0% (L0), 10% (L1) dan 20% (L2). Perendaman dilakukan selama 6 jam, kemudian dihitung jumlah bakteri dan diukur tingkat penerimaan konsumen terhadap ikan bandeng. Hasil penelitian menunjukkan bahwa penggunaan ekstrak lengkuas pada perendaman ikan bandeng berpengaruh terhadap penurunan jumlah bakteri. Pada tingkat penerimaan konsumen, penggunaan ekstrak lengkuas pada perendaman ikan bandeng berpengaruh nyata terhadap kenampakan mata tetapi tidak berpengaruh nyata pada bau, warna insang dan tekstur.  Konsumen menyukai bau, warna insang dan tekstur setelah perendaman ekstrak lengkuas, namun kenampakan mata dan warna insang dengan perendaman lengkuas konsentrasi 20% kurang disukai oleh konsumen.This study aimed to determine the effect of galangal extract on immersion milkfish to the number of bacteria and the level of consumer acceptance. This study used 27 milkfish are grouped into 3 groups, each consisting of nine milkfish. Third consecutive group soaked in a solution of 0% (L0), 10% (L1) and 20% (L2) galangal extract concentration. Immersion performed for 6 hours and then counted the number of bacteria and measured the level of consumer acceptance of the milkfish. The results showed that the use of extract of galangal on milkfish immersion effect on decreasing the number of bacteria. At the level of consumer acceptance, the use of galangal extract on milkfish immersion significantly affect the appearance of the eye but had no significant effect on smell, color and texture gills. Consumers love the smell, color and texture after soaking gills galangal extract, but the appearance of the eyes and the color of the gills with ginger dipping concentration of 20% less favored by consumers.
Etnobotani Tumbuhan Obat Masyarakat Desa Keseneng Kecamatan Sumowono Kabupaten Semarang Jawa Tengah
Life Science Vol 1 No 2 (2012)
Publisher : Universitas Negeri Semarang

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Abstract

Penelitian ini bertujuan menginventarisasi potensi etnobotani tumbuhan obat oleh Masyarakat Keseneng yang meliputi jenis tumbuhan obat, cara memperoleh tumbuhan obat, penyakit yang dapat disembuhkan, bagian tumbuhan yang digunakan dan cara penggunaannya. Penelitian ini menggunakan dua tahapan.Tahap pertama, penggalian potensi Desa Keseneng sebagai tempat penelitian dengan wawancara terbuka.Tahap kedua yaitu pengambilan data, meliputi data utama yaitu data tumbuhan obat dan data penunjang berupa data informan dengan wawancara semi terstruktur.Hasil penelitian mengungkap bahwa masyarakat Keseneng menggunakan 31 jenis tumbuhan obat dari 21 famili.Tumbuhan obat didapat dari hutan (15 jenis), pekarangan rumah (13 jenis), tepi jalan (5 jenis), tepi sawah (5 jenis), sawah (2 jenis) dan tepi sungai (1 jenis).Tumbuhan obat di desa Keseneng dapat mengobati 15 kelompok penyakit dengan bagian tumbuhan obat yang digunakan yaitu rimpang (7 jenis), semua bagian tumbuhan (3 jenis), batang (3 jenis), buah (7 jenis), daun (10 jenis), biji (1 jenis), getah (3 jenis) dan akar (2 jenis). Berdasarkan pengolahannya tumbuhan obat dibagi menjadi 4, yaitu dimanfaatkan dalam bentuk segar (18 jenis), direbus (13 jenis), dikeringkan (4 jenis) dan dilayukan/dibakar (2 jenis).This study aims to inventory the potential of medicinal plants ethnobotany by the Keseneng Society which includes the species of medicinal plants, how to obtain medicinal plants,curable disease, plant parts used and how to use them. This study uses two stages. The first stage is improving the potential of Keseneng Village as the place to study with an open interview. The second stage is data collection, including the main data, they are medicinal plants, and the supporting data in the form of data informant with a semi-structured interviews. The results show that the Keseneng using 31 types of medicinal plants from 21 families. Medicinal plants were from forest plants (15 types), yard (13 types), the edge of road (5 types), the edge of field (5 types), field (2 types) and river (1 species). Medicinal plants in the Keseneng village can treat 15 types of disease with the parts of plant used, such as the rhizome (7 types), all parts of the plant (3 types) , trunk (3 types), Fruit (7 types), leaves (10 types), seeds (1 types), latex (3 types) and root (2 types). Based on its processing, medicinal plants were divided into 4, which are utilized in fresh form (18 types), boiled (13 types), dried (4 types) and withered / burned (2 types). 
PENGARUH VITAMIN E TERHADAP KADAR SGPT DAN SGOT TIKUS PUTIH GALUR WISTAR YANG DIPAPAR TIMBAL
Life Science Vol 2 No 1 (2013)
Publisher : Universitas Negeri Semarang

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Abstract

This study aimed to examine the effect of the vitamin E administration on levels of SGOT and SGPT blood serum male albino rats (Rattus norvegicus) wistar strain were exposed of lead per oral. The sample used 20 male rats were divided into 4 groups:I of a control, II of the treatment of lead 175 mg/rat, III of Vitamin E 1.44 mg/rat and lead 175 mg/rat, IV of Vitamin E 2.16 mg/rat and lead 175 mg/rat for 21 days. On day 22 measured levels of SGOT and SGPT and blood lead. SGOT and SGPT data were analyzed with one-way ANOVA, followed by BNT test. The results of one-way ANOVA showed antioxidant vitamin E giving a significant effect on the levels of SGOT and SGPT (p <0.05) rats. The BNT test SGOT levels showed a significant difference except in group II and III, the SGPT levels showed a significant difference except in group III and IV. From the results of this study concluded that the affects of vitamin E decrase levels of SGOT and SGPT blood serum of male albino rats (Rattus norvegicus) wistar strain were exposed to lead per oral.
DISTRIBUSI LONGITUDINAL BERBAGAI SPESIES IKAN DI SUNGAI DAMAR KABUPATEN KENDAL
Life Science Vol 2 No 1 (2013)
Publisher : Universitas Negeri Semarang

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Abstract

Damar River receives waste inputs from household industrial activity and agricultural activity that can effect on the distribution and the diversity of living things in it, including the fish. This research is exploration research, with the sampling was conducted at 5 stations in different locations. The samples were collected at 3 different sampling times with 2-week interval. Data was analysed using longitudinal distribution, based on the density of species per-station and per-period of sampling. The species diversity was also calculated using Shanon Diversity Index. There were 10 fish species that were distributed in the Damar River i.e. Anabas testudineus, Trichogaster trichopterus, Clarias batracus, Oreochormis niloticus, Arius caelatus, Periophtalmus malacensis, Scatophagus argus, Crenimugil heterocheilos, Pomadasys argenteus, and Sillago robusta. Species which was distributed along the Damar River was Anabas testudineus with the distribution center at river estuary. The Diversity Index value of Damar River is categorized as intermediate (1.88), thus it shows that the water quality criteria of Damar River is lightly polluted water.

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