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Life Science
Published by Universitas Negeri Semarang
ISSN : 22526277     EISSN : 25285009     DOI : https://doi.org/10.15294/lifesci
Core Subject : Agriculture,
Agriculture, Biological Sciences & Forestry
Life Science publishes original and significant articles on all aspects of Life Sciences (Biology, Genetics, Biological Anthropology, Botany, Medical Sciences, Veterinary Sciences, Biochemical Genetics, Biometry, Clinical Genetics, Cytogenetics, Genetic Epidemiology, Genetic Testing, Evolution and Population Genetics, Immunogenetics and Molecular Genetics). The journal also covers ethical issues. It aims to serve as a forum for life scientists and health professionals.
Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Pertanian dan Biologi (Lain-Lain)
Articles 219 Documents
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Sinergisme Isolat Potensial Biofertilizer Bacillus cereus yang Dikonsorsiumkan Muhammad Fajar; Siti Khotimah; Rahmawati Rahmawati
Life Science Vol 12 No 2 (2023): November 2023
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/lifesci.v12i2.73792

Abstract

A consortium of microorganisms is a mixture of microbial populations in the form of a community that has cooperative, commensal, and mutualistic relationships. Naturally, bacteria are able to communicate with each other. The relationship between bacteria can have a synergistic relationship if the substrate is sufficient and the bacteria do not inhibit each other's growth. In this study, Bacillus cereus isolates SP1, SP2, and SP3 isolated from Sungai Ambawang peatland, Kubu Raya Regency, West Kalimantan will be consorted. The purpose of this study was to determine the synergism of three strains of Bacillus cereus bacteria. All isolates tested for synergism against other isolates showed no inhibition zone at the intersection between isolates. This means that each isolate can synergize with one another. The synergism test results show that three strains of Bacillus cereus bacteria can synergize in the manufacture of biofertilizers.
Potensi Antagonis Bakteri Pelarut Fosfat Asal Tanah Gambut terhadap Bakteri Xanthomonas sp. Penyebab Kanker Daun Tanaman Jeruk Siam Pontianak Juniarti, Shella Mida; Khotimah, Siti; Rahmawati, Rahmawati; Mukarlina, Mukarlina
Life Science Vol 13 No 1 (2024): May 2024
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/lifesci.v13i1.74174

Abstract

The productivity of Siam citrus in West Kalimantan from year to year has begun to decline, one of the causes is a disease that attacks the plant. One of the diseases that often attack citrus plants is leaf cancer disease caused by the bacterium Xanthomonas sp. Phosphate solubilizing bacteria are one of the rhizosphere bacteria from peat soils that can act as biological control agents in plants. Therefore, this study aimed to determine phosphate solubilizing bacteria from peat soil as a control for leaf cancer of the Siam Pontianak citrus plant caused by Xanthomonas sp. This research was conducted from June 2022 to September 2022, at the Microbiology Laboratory, Department of Biology, Faculty of Mathematics and Natural Sciences, Tanjungpura University, Pontianak. This study used a completely randomized design (CRD) with each of the 5 treatment levels repeated 3 times to obtain 15 experimental units. Antagonist test in vitro using the dual culture method. The results showed that all phosphate solubilizing bacteria from peat soil Bacillus sp. (SGB1), Bacillus sp. (SGB 2), and Bacillus sp. (SGB 3) have antagonistic potential against Xanthomonas sp. (BP2).
Efektivitas Ekstrak Daun Kelor Merah sebagai Usaha Preventif terhadap Perkembangan Sel B (SDF-1) dan Sel Granulosit (Gr-1) pada Mencit Balb/c yang diinjeksi Salmonella typhi Maria Magdalena Riyaniarti Estri Wuryandari; Hartati Tuna
Life Science Vol 12 No 2 (2023): November 2023
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/lifesci.v12i2.74290

Abstract

An infection caused by Salmonella typhi can cause a reaction to DNA damage, causing a delay in the cell cycle, either temporarily or permanently, and can also cause cell death and a permanent delay in the cell cycle, causing a decrease in the self-renewal ability of haematopoetic stem cells, causing continued damage. in hematopoietic cells. Improving the immune system in the body can be done by administering synthetic drugs in the form of antibiotics or giving herbal supplements. Red Moringa has the ability as an immunostimulant and immunomodulator. The research aims to determine the effectiveness of Moringa leaf extract on the expression of B cell and granulocyte cell development due to exposure to Salmonella Typhi. The research design used experimental research using treatment groups and non-treatment groups in mice injected with salmonella typhi. Data from flow cytometry were analyzed using BD Cellquest ProTM software (%) and first transformed using arc sin transformation. The arc sin transformation results were analyzed using one-way ¬ANOVA at a confidence level of 95% (α=0.05). Data are presented in the form of mean ± standard error (SE). The results of this research, the Moringa leaf extract dose of 42mg/BW is an effective dose to restore the relative number of B cells and granulocyte cells to normal conditions.
Analisis Struktur dan Profil Vegetasi Mangrove di Kecamatan Sayung Kabupaten Demak ALIFIANSYAH, JUANDRA; Irsadi, Andin; Kartijono, Nugroho Edi; Ngabekti, Sri
Life Science Vol 13 No 1 (2024): May 2024
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/lifesci.v13i1.74475

Abstract

Sayung District is one of the district in Demak Regency which has mangrove vegetation. The alteration of Demak Regency's coastal areas caused by the sea rob phenomenon has a significant impact on the mangrove vegetation. The Semarang-Demak toll road construction along Sayung coast potentially causes mangrove damage. The purpose of this study is to determine the structure and profile, and analyze the condition of mangrove vegetation in Sayung, based on the structure and profile of Sayung mangrove vegetation. The study was conducted on the mangroves of Bedono, Mondoliko, and Morosari Village. This research used an exploratory method with descriptive analysis, by collecting data on vegetation parameters and vegetation profiles. The results showed that the types of mangroves found at all observation stations consisted of Avicennia, Bruguiera, and Rhizophora. The profile graph shows an association between Avicennia alba, Avicennia marina, and Rhizophora mucronata. Important Value Index (IVI) for all stations shows that Avicennia officinalis have highest value on 65,30 and 50,18 respectively. Diversity index shows that both stations have value on 0,873 and 0,893 which belongs to low diversity. Similarity index on each sub-stations shows the significant difference between Bedono-A, Bedono-C, Mondoliko, and Morosari stations at 0,4 index, Based on Abiotic parameters such as pH, salinity, and water temperature found on the optimal quality standard level. The conclusion is mangrove vegetation structure dominated by Avicennia and Rhizophora groups, with a low level of diversity at all observation stations.
Pertumbuhan Maggot Hermetia illucens L. pada Media Kombinasi Bungkil Kelapa Sawit yang Difermentasi dan Dedak Padi Yanti, Ari Hepi; Rahmanisya, Afifah; Saputra, Firman
Life Science Vol 13 No 1 (2024): May 2024
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/lifesci.v13i1.75978

Abstract

Maggot Hermetia illucens is the larval phase of the black soldier fly. This study aims to analyze the development of maggots in the form of length, body weight and population density in a combination of fermented oil palm meal and rice bran. The study used a Complete Randomized Design (RAL) with 5 treatments and 5 repeats for 21 days, namely 100% oil palm meal, 75% oil palm meal and 25% rice bran, 50% oil palm meal and 50% rice bran, 25% oil palm meal and 75% rice bran, and 100% rice bran. Random sampling is used for data collection. The length of the maggot's body is measured using a digital caliper. The total weight of the maggots on each treatment on the 21st day was weighed for weight measurement. Density is measured by counting the number of individuals per volume of media. Data were analyzed with single-track ANOVA and Tukey test. The results showed significant growth of Hermetia illucens maggots (p = 0.000) using a combination of fermented oil palm meal media and rice bran. The combination of 75% oil palm meal and 25% rice bran has a significant effect with the highest length for 21 days at 27.56 mm, a total weight of 34.86 grams. Its population density is insignificant.
Analisis Perbandingan Kadar Hemoglobin, Jumlah dan Struktur Eritrosit pada Lima Kelas Vertebrata Jabar, Muhammad Abdul; Ramadhanti, Shifa; Purnamaningrum, Sisilia Prita Dewi; Christijanti, Wulan
Life Science Vol 12 No 2 (2023): November 2023
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/lifesci.v12i2.76310

Abstract

The size, shape of cells, number of erythrocytes and hemoglobin content between each class of animal can be different. This study aims to examine the comparison of hemoglobin levels, number and structure of erythrocytes in five classes of vertebrates. The animal blood used in this study consisted of five species representing each class, namely Clarias batrachus representing the pisces, Bufo sp. represents the amphibian, Varanus salvator represents the reptile, Columba livia represents the aves, and Mus musculus represents the mammal. Hemoglobin levels were tested using the Sahli method, while the number and structure of erythrocytes used a hemocytometer and Neubauer counting chamber which were observed under a microscope with a magnification of 40x10. The results showed that hemoglobin levels in Clarias batrachus, Bufo sp., Varanus salvator, Columba livia, and Mus musculus were 3.8 g/dl, 6.8 g/dl, 10.4 g/dl, 10 .8 g/dl, and 8.2 g./dl. The number of erythrocytes was 780,000/mm3, 320,000/mm3, 1,900,000/mm3, 3,270,000/mm3, and 3,960,000/mm3, respectively. In terms of erythrocyte structure, it was found that Clarias batrachus has a round structure with a nucleus, Bufo sp., Varanus salvator, and Columba livia have an oval shape with a nucleus, while Mus musculus has a biconcave disc shape without a nucleus. The conclusion of this study shows that the highest hemoglobin levels are found in Columba livia and the lowest in Clarias batrachus. The highest number of erythrocytes was found in Mus musculus, and the lowest in Bufo sp. All erythrocytes in the vertebrate class have a nucleus, except in mammals
Daftar Isi Life Science 12 (2) 2023 Life Science
Life Science Vol 12 No 2 (2023): November 2023
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/lifesci.v12i2.76728

Abstract

Daftar Isi Life Science Edisi 12 (2) 2023: November
Kadar Leptin Balita Stunting yang Diberi Makanan Tambahan Berbasis Daun Kelor dan Ikan Laut Fatiah, Nirmala Al; Yuniastuti, Ari; WH, Nugrahaningsih; Lisdiana, Lisdiana
Life Science Vol 13 No 1 (2024): May 2024
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/lifesci.v13i1.76891

Abstract

Leptin plays a role in regulating the growth of bones and other body tissues. Leptin can affect the epiphyseal growth zones in long bones, contributing to height growth. The aim of this research is to analyze leptin levels and body height in toddlers before and after giving additional food based on Moringa leaves and sea fish. The supplementary feeding intervention was carried out for 10 weeks on 30 toddlers with stunted and very stunted nutritional status at the Sedan Community Health Center, Rembang Regency. Leptin levels in the blood were analyzed using ELISA, height measurements were carried out using a stadiometer, determination of nutritional status was guided by the PERMENKES regarding 2020 child anthropometry standards, and data analysis used the SPSS program. The results of the study showed that the average leptin level decreased and the average body height increased after the intervention of providing additional food based on Moringa leaves and sea fish. Thus, it can be concluded that decreasing leptin levels can cause a decrease in fat and adipogenesis which results in increased bone formation in the form of increased height in stunted toddlers at the Sedan Community Health Center, Rembang Regency.
Analisis Kecepatan Pertumbuhan Pohon Potensial untuk Restorasi Lahan Kritis Gunung Ungaran Billa, Alfiah Maula Salsa; Zukhoiri, Shinta Fatma; Saufatikha, Nasytha Saski; Nadifa, Jasmine Augi; Akbar, Bintang Faisal; Irsadi, Andin
Life Science Vol 13 No 1 (2024): May 2024
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/lifesci.v13i1.77493

Abstract

Penelitian ini dilakukan di Gunung Ungaran untuk mendukung program konservasi dan kelestarian daerah tersebut yang mengalami peningkatan lahan kritis akibat alih fungsi lahan oleh petani kopi. Penelitian dilakukan dari Mei hingga Oktober 2023 dengan metode survei vegetasi. Penelitian ini bertujuan untuk mengetahui pohon potensial untuk ditanam di lahan kritis berdasarkan kecepatan pertumbuhanya. Hasil penelitian menunjukkan bahwa jenis-jenis pohon yang paling potensial untuk ditanam di lahan kritis berdasarkan kecepatan pertumbuhan tinggi adalah Engelhardia serrata, Homalanthus populneus, dan Bischofia javanica. Berdasarkan kecepatan pertumbuhan diameter batang, bibit yang potensial adalah Peleman, Castanopsis argentea, dan Wuru Kopi. Selain itu, berdasarkan analisis vegetasi, jenis-jenis pohon paling potensial untuk restorasi lahan berdasarkan nilai Indeks Nilai Penting (INP) adalah Litsea sp. dan Ndog-ndogan pada strata 1, Parastemon urophyllus pada strata 2, dan Castanopsis argantea pada stratifikasi 3. Jenis-jenis pohon ini direkomendasikan untuk ditanam di lahan kritis Gunung Ungaran karena memiliki tingkat pertumbuhan dan kesuksesan yang tinggi. Upaya ini penting untuk memitigasi ancaman terhadap keanekaragaman hayati dan sumber air di Gunung Ungaran serta untuk mendukung masyarakat yang bergantung pada gunung ini.

Page 22 of 22 | Total Record : 219

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