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Life Science
Published by Universitas Negeri Semarang
ISSN : 22526277     EISSN : 25285009     DOI : https://doi.org/10.15294/lifesci
Core Subject : Agriculture,
Agriculture, Biological Sciences & Forestry
Life Science publishes original and significant articles on all aspects of Life Sciences (Biology, Genetics, Biological Anthropology, Botany, Medical Sciences, Veterinary Sciences, Biochemical Genetics, Biometry, Clinical Genetics, Cytogenetics, Genetic Epidemiology, Genetic Testing, Evolution and Population Genetics, Immunogenetics and Molecular Genetics). The journal also covers ethical issues. It aims to serve as a forum for life scientists and health professionals.
Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Pertanian dan Biologi (Lain-Lain)
Articles 219 Documents
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KEANEKARAGAMAN FITOPLANKTON DI ALIRAN SUMBER AIR PANAS CONDRODIMUKO GEDONGSONGO KABUPATEN SEMARANG
Life Science Vol 2 No 1 (2013)
Publisher : Universitas Negeri Semarang

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Abstract

Condrodimuko Gedongsongo is one of the hot water sources where placed at north of Ungaran mountain in Kabupaten Semarang. The hot temperature and mineral content of the hot waters only tolerant phytoplankton that can be survive in this condition. The aim of this research was to know the species diversity of phytoplankton in the flow of hot waters in Condrodimuko Gedongsongo this condition. Selected sampling purpossive sampling at three stations, each station do three sampling period. The species number and the individual number of the phytoplankton were taken as data to conclude the diversity species of phytoplankton using Shannon-Wiener method. The result of the research was nine species of phytoplankton were found there are Cladopora sp, Gonatozygon sp, Microspora sp, Oscillatoria sp, Phormidium sp, Protococcus sp, Tribonema sp, Diatom sp dan Ophiocytium sp. The result of analysis on phytoplankton diversity of hot water sources at the Condrodimuko Gedongsongo Kabupaten Semarang the value 2,09 and is one of high diversity.
UJI RESISTENSI Mycobacterium tuberculosis TERHADAP OBAT ANTI TUBERKULOSIS (OAT) DENGAN METODE PENIPISAN
Life Science Vol 2 No 1 (2013)
Publisher : Universitas Negeri Semarang

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Abstract

The purpose of this research is to find out the reaction of M. tuberculosis resistance to OAT whit dilution method. This research is exploration research with post randomized controlled design. Each sample made as suspension with Mc Farland 1 turbidity then experimented with PNB to detect Mycobacterium Other Than Tuberculosis (MOTT). Negative result of PNB experiment then resistance experimented. Resistance experiment grown in Lowenstein-Jeensen (L-J) medium as control and were mixed is L-J medium which had been added with each OAT that is Isoniazid 0,2 mg/L, Rifampisin 40 mg/L, Streptomisin 4 mg/L dan Etambutanol 2 mg/L. Experiment result of PNB shows that there are five negative sample and one MOTT positive sample. Resistance experiment with dilution shows that there are 5 sensitive samples to OAT. From the research it can be concluded that by dilution method is not really influencing M. tuberculosis resistance experiment to OAT.
SAPROBITAS PERAIRAN SUNGAI JUWANA BERDASARKAN BIOINDIKATOR PLANKTON
Life Science Vol 2 No 1 (2013)
Publisher : Universitas Negeri Semarang

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Abstract

Sungai Juwana merupakan sungai terbesar dan terpanjang di Kota Pati. Berkembangnya kegiatan penduduk di Daerah Aliran Sungai (DAS) Juwana dapat mempengaruhi kualitas air sungai. Penelitian ini menggunakan rancangan eksplorasi dengan metode survai, dan penetapan stasiun pengambilan sampel dengan metode purposive sampling. Penempatan stasiun didasarkan atas perkiraan beban pencemar yang masuk ke sungai. Stasiun 1 berada hulu sebagai pembanding, stasiun 2 sumber limbah pabrik tahu dan pertanian, stasiun 3 sumber limbah industri kacang, stasiun 4 sumber limbah industri peleburan timah, stasiun 5 limbah ikan dari TPI serta pelabuhan, dan stasiun 6 berada di hilir sungai (dekat muara). Pengambilan sampel dilakukan sebanyak 3 kali dengan selang waktu 2 minggu. Hasil penelitian ditemukan 10 jenis plankton terdiri dari 3 jenis kelompok α-Mesosaprobik, 2 jenis kelompok β-Mesosaprobrik dan 5 jenis kelompok non saprobik, kemudian diinterpretasikan pada tabel hubungan antara koefisien saprobitas perairan dengan tingkat pencemaran perairan. Berdasarkan perhitungan dan analisis nilai koefisien saprobik dari stasiun 1 sampai hilir didapatkan nilai koefisien saprobik plankton berkisar antara -0,6 s/d -1. Berdasarkan kriteria tingkat pencemaran menunjukkan bahwa Sungai Juwana berada dalam kondisi tercemar sedang sampai dengan cukup berat.Juwana River is the largest and longest river in the city of Pati. The developing activities in the watershed Juwana can affect water quality. Exploratory study used a survey method, where the determination of sampling stations using purposive sampling. The settlement of the stations was conducted based on the estimated pollutant loads into the river, the first station was located in the upstream of the river, as comparison the second station was located nearby peanut industry, next the fourth station was located in the tinmelting industrial wastes, the fifth station was located in the fish market and nearby the Harbour, the last , sixth station was located in the downstream of the river. Sampling performed 3 times with an interval of 2 weeks. In this research, it has found that is 10 species of plankton consist of 3 species group α-Mesosaprobic, 2 species group β-Mesosaprobrik and 5 species group non saprobik, and than interpreted the relationship between the coefficient table saprobitas waters with levels of water pollution. Based on the calculation and analysis of the saprobic coefficient values station 1 to downstream obtainet plankton saprobic coefficient values ranged from -0.6 s / d -1. Based on these criteria indicate that the level of pollution in Juwana River is moderate to high.
PERKEMBANGAN OVARIUM BURUNG PUYUH YANG DIBERI VARIASI PENCAHAYAAN WARNA LAMPU SELAMA 16 JAM
Life Science Vol 2 No 1 (2013)
Publisher : Universitas Negeri Semarang

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Burung puyuh merupakan salah satu unggas yang sedang dikembangkan dan ditingkatkan produksinya. Untuk meningkatkan produksinya, berbagai pemeliharaan telah dilakukan. Salah satunya dengan memberikan program pencahayaan. Tujuan penelitian ini adalah untuk mengetahui perkembangan ovarium burung puyuh yang diberi variasi warna cahaya lampu dan warna cahaya yang paling optimal terhadap perkembangan ovarium. Burung puyuh (Coturnix-coturnix japonica) betina sebanyak 25 ekor berumur lima hari diaklimasi selama satu minggu. Selanjutnya dikelompokan menjadi lima perlakuan, masing-masing kelompok terdiri atas lima ekor puyuh yaitu kelompok kontrol menggunakan cahaya matahari, kelompok I cahaya lampu bening, kelompok II merah, kelompok III biru, dan kelompok IV kuning. Perlakuan cahaya diberikan selama 16 jam per hari (06.00-22.00 WIB) selama 30 hari. Selanjutnya burung puyuh dibedah dan diamati perkembangan ovarium yang meliputi berat badan burung puyuh, berat ovarium dan struktur makroskopis ovarium. Pemberian variasi warna lampu pencahayaan selama 30 hari dengan 16 jam ternag/hari berpengaruh terhadap perkembangan ovarium burung puyuh dan pencahayaan warna lampu merah merupakan pencahayaan yang paling optimal. Quail is one of poultry that are being developed and enhanced its production. To increase production, a variety of maintenance has been carried out. One of them is by giving the program the lighting. The purpose of this research is to know the development of quail ovarian given color variations of light and color the most optimal light towards the development of ovaries. Quail (Coturnix-coturnix japonica) females as much as 25 five-day-old tail diaklimasi for one week. Next up are grouped into five treatment groups, each consisting of five quail tail which is the control group using sun light, the light group I group II, red, clear, blue, group III and Group IV yellow. Light treatment is given for 16 hours per day (6 am-10 pm) for 30 days. Quail Furthermore dissected and observed the development of the ovary which include weight, the weight of the quail ovary and ovarian makroskopis structure. Provision of lighting lamp color variation for 30 days with 12 hours of light/day affect the ovarian development of quail and lighting color red lights is the most optimal lighting.
POPULASI JULANG EMAS (Aceros Undulatus) DI GUNUNG UNGARAN JAWA TENGAH
Life Science Vol 2 No 1 (2013)
Publisher : Universitas Negeri Semarang

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Penelitian ini bertujuan mengetahui Populasi Julang Emas di Gunung Ungaran. Data populasi diperoleh dengan menggunakan Metode Variable Circular Plot (VCP) dengan jarak radius pengamatan 1 Km (Luas VCP 0,535 Km²). Penelitian dilakukan pada bulan Juli- Agustus 2011 dan Januari 2012. Pengambilan data dimulai pukul 06.00- 16.00 WIB. Hasil penelitian menunjukanpopulasi Julang Emas di Gunung Ungaran adalah 15 ekor/Km². Populasi Julang Emas di Gunung Ungaran tersebar merata di empat stasiun dengan kepadatan populasi distasiun Medini (19,21 ekor/Km²), stasiun Banyuwindu (15,66 ekor/Km²), stasiun Gajah Mungkur (12,48 ekor/Km²) dan stasiun Gunung Gentong (10,5 ekor/Km²). Perbandingan rasio kelamin antara jantan dan betina yaitu 1:1 menunjukkan populasi Julang Emas di Gunung Ungaran berada pada musim tidak berbiak.The objective of this study was to determine the Wreathed Hornbill population at Mount Ungaran. Population data obtained using VCP (Variable Circular Plot) with 1 km diameter of circular plot (0.535 Km²). The study was conducted from July-Agustus 2011 and January 2012. The Observation started at 06:00 am to 04:00 pm. The results showed that the population of Wreathed Hornbill in Mount Ungaran was 15 individual/Km². The population of Wreathed Hornbill distributed was four station that were Medini station (19 individual/Km²), Banyuwindu station (15 individual/Km²), Gajah Mungkur station (12 individual/Km²) and Gunung Gentong station (10 individual/Km²). Sex ratio between male and female was 1:1, that showed the Wreathed Hornbill was not in breeding season.
MIKRO ANOTOMI INSANG IKAN SEBAGAI INDIKATOR PENCEMARAN LOGAM BERAT DI PERAIRAN KALIGARANG SEMARANG
Life Science Vol 2 No 1 (2013)
Publisher : Universitas Negeri Semarang

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PerairanKaligarangmerupakanperairanyangsangatpentingbagikehidupanpendudukdiKotaSemaranSelain masyarakat, ndutri-industri di sepanjang perairan tersebut juga menggunakan air PerairanKaligarang untuk keperluan proses produksi, sekaligus sebagai tempat pembuangan limbah padaakhir proses produksinya.Ikan sebagai salah satu biota air sering dijadikan sebagai salah satuindikator tingkat pencemaran yang terjadi di dalam perairan.Penelitian ini menggunakan rancanganeksplorasi karena melihat dari tingkatan pencemaran logam berat dengan menggunakan ikansebagai indikator. Pengambilan sampel diambil setiap 2-3 minggu sekali dalam jangka waktu selama1 bulan pada bulan November-Desember 2012. Data dalam penelitian ini adalah data kualitatifberupa tingkat kerusakan mikroantomi insang ikan yang terindikasi logam berat kemudiandihubungkan dengan faktor lingkungan meliputi Suhu, pH, BOD, COD.Berdasarkan pengamatanmikroanatomi insang ikan yang terindikasi logam berat terjadi kerusakan mikroanatomi sampai ketingkat nekrosis. Berdasarkan kriteria tingkat pencemaran menunjukkan bahwa Perairan Kaligarangberada dalam kondisi tercemar sedang sampai dengan cukup berat.Kaligarang river is the river which is very important to the life of residents in the city of Semarang. In additionto the community, industry was-industry along the river is also used Kaligarang River water for the productionprocess, as well as the disposal of waste at the end of the production process. Fish as one of the aquatic biota isoften used as an indicator of the level of contamination that occurred in the waters. This study used exploratorydesign as seen from the level of heavy metal pollution using fish as indicators. Sampling was taken every 2-3weeks for a period of one month in November-December 2012. The data in this study was qualitative data in theform of the damage fish gills mikroantomi indicated heavy metals were then linked to environmental factorsinclude temperature, pH, BOD, COD.Based on observations of fish gills mikroanatomi indicate mikroanatomidamage to the level of necrosis. Based on these criteria indicated that the level of pollution in a state Kaligarangriver polluted with moderate to heavy.
INDUKSI PERBANYAKAN TUNAS Rosa damascena Mill. DENGAN PENAMBAHAN AUKSIN DAN SITOKININ
Life Science Vol 2 No 1 (2013)
Publisher : Universitas Negeri Semarang

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Penelitian ini bertujuan untuk mengetahui optimasi auksin dan sitokinin pada induksi perbanyakan tunas mawar damaskus. Penelitian perbanyakan tunas menggunakan rancangan acak lengkap faktorial terdiri dari dua faktor yaitu variasi konsentrasi BA (1, 2, 3, dan 4 ppm) dan konsentrasi NAA (0,1; 0,3; 0,5 dan 0,7 ppm). Tunas lateral diinduksi pada media Murashige & Skoog dengan penambahan kombinasi BA dan NAA (16 kombinasi) selama enam minggu, yang diukur berdasarkan dua parameter yaitu rentang waktu muncul tunas pertama dan jumlah tunas. Data dianalisis menggunakan anava dua jalan dan uji Duncan. Hasil penelitian menunjukkan BA, NAA dan kombinasinya berpengaruh terhadap rentang waktu muncul tunas pertama, tetapi hanya BA yang berpengaruh terhadap jumlah tunas. Hasil analisis data menunjukkan bahwa kombinasi BA 3 ppm+NAA 0,3 ppm optimal untuk induksi rentang waktu muncul tunas pertama yakni 4 hari MST, sedangkan jumlah tunas terbanyak dihasilkan oleh BA 1 ppm dengan 7 tunas.This research aimed to know the optimation of auxin and cytokinin on damask rose multiple shoot induction. Shoot multiplication did by fledged random design factorial consist of two factors those were various BA concentration (1, 2, 3, and 4 ppm) and NAA concentration (0.1; 0.3; 0.5 and 0.7 ppm). Axillary shoots were induced on Murashige & Skoog medium supplemented with various BA and NAA combinations (16 combinations) for six weeks, measured based on two parameters, shoot initial timing and shoot numbers. Data was analized by two way anova and DMRT. The result showed that BA, NAA and it’s combination were significantly influenced on shoot initial timing induction, but only BA was significantly influenced on shoot numbers. Based on data analysis, 3 ppm+NAA 0,3 ppm BA combination was optimal for shoot initial timing induction in 4 days after plant and 1 ppm BA gave the highest shoot numbers with 7 shoots per nodal.
IDENTIFIKASI EKTOPARASIT PADA IKAN KOI (Cyprinus carpio L) DI PASAR IKAN HIAS JURNATAN SEMARANG
Life Science Vol 2 No 2 (2013)
Publisher : Universitas Negeri Semarang

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Pasar ikan identik dengan tempat penampungan ikan yang terbatas, kepadatan ikan yang tinggi serta kurangnya perhatian mengenai pemeriksaan ikan yang dijual mengakibatkan ikan mudah terkena parasit. Serangan ektoparasit dapat mengakibatkan ikan kehilangan nafsu makan, kemudian perlahan-lahan lemas dan berujung kematian. Tujuan penelitian ini adalah untuk mengidentifikasi jenis ektoparasit pada ikan koi yang terdapat di Pasar Ikan Jurnatan Semarang. Ikan koi (Cyprinus carpio L) sebanyak 54 ekor diambil dari 6 stasiun (pedagang ikan). Pengambilan ikan tiap stasiun sebanyak sembilan ekor berukuran 7-14 cm diambil secara acak. Pemeriksaan ikan dilakukan dengan mengerok bagian kulit kepala sampai ekor menggunakan scalpel hingga mendapatkan lendir. Lendir diletakkan pada gelas benda dan diamati dibawah mikroskop. Identifikasi ektoparasit pada bagian insang ikan dilakukan dengan memotong bagian insang menggunakan gunting kemudian diletakkan pada gelas benda dan diamati di bawah mikroskop. Hasil pemeriksaan ditemukan ektoparasit yang menginfeksi ikan koi adalah Trichodina sp, Dactylogyrus sp, Gyrodactylus sp, Argulus sp dan Myxobolus sp. The fish market is identical to the limited fish shelters, high fish density and lack of attention on checking the fish sold resulted susceptible fish parasites. Ectoparasites attack can result in fish loss of appetite, then slowly limp and lead to death. The purpose of this study was to identify the types of fish koi ectoparasites in Jurnatan Semarang fish market. 54 Koi Fish (Cyprinus carpio L) were taken from 6 stations (fish traders). Nine fishes were taken randomly from each fishing station with 7-14 cm in lenght. Ectoparasite checked by scraping the fish skin from head to the tail section using a scalpel to get the mucus. Mucus was placed on a glass object and observed under a microscope. Identification of ectoparasites on the gills of the fish was done by cutting the gills with scissors and then placed on a glass object and observed under a microscope. The results found ectoparasites that infect koi fish was Trichodina sp, sp Dactylogyrus, Gyrodactylus sp, Argulus sp and Myxobolus sp.
UJI EFEKTIVITAS EKSTRAK BAWANG PUTIH (Allium sativum Linn.) SEBAGAI ANTIBAKTERI PADA IKAN BANDENG (Chanos chanos Forsk.) SEGAR
Life Science Vol 2 No 2 (2013)
Publisher : Universitas Negeri Semarang

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Penelitian ini bertujuan mengetahui konsentrasi ekstrak bawang putih yang paling efektif sebagai antibakteri dalam menghambat pertumbuhan bakteri yang diisolasi dari ikan bandeng serta lama simpan maksimal ikan bandeng dengan perlakuan pemberian ekstrak bawang putih. Penelitian ini merupakan penelitian eksperimental menggunakan rancangan acak lengkap dua arah, menggunakan kelompok kontrol dan kelompok perlakuan konsentrasi ekstrak bawang putih: 5%,10%,15% dan waktu penyimpanan setelah perendaman selama 6 jam, 12 jam, 24 jam, 48 jam. Data jumlah koloni bakteri dianalisis dengan Anava dan uji lanjut dengan BNT (Beda Nyata Terkecil). Hasil penelitian menunjukkan ekstrak bawang putih efektif menghambat pertumbuhan bakteri yang diisolasi dari daging ikan bandeng. Hasil Anava menunjukkan konsentrasi 10% dan 15% signifikan dalam penghambatan jumlah koloni bakteri serta waktu penyimpanan selama 24 jam merupakan lama waktu maksimal setelah diberi perlakuan dibandingkan dengan kontrol. Berdasarkan hal ini dapat disimpulkan pemberian ekstrak bawang putih efektif menghambat pertumbuhan bakteri dengan konsentrasi paling efektif 10% serta waktu penyimpanan maksimal adalah 24 jam.This study aims to determine the most effective concentration of garlic extract as an antibacterial in inhibiting the growth of bacteria isolated from milkfish and long shelf maximum milkfish treated with garlic extract. This study was an experimental study using a completely two-way randomized design, used a control group and a treatment group of garlic extract concentrations: 5%, 10%, 15% and storage times after soaking for 6 hours, 12 hours, 24 hours, 48 hours. Data on the number of bacterial colonies were analyzed by ANOVA and further tests with BNT (Least Significant Difference). The results showed garlic extracts effectively inhibited the growth of bacteria isolated from milk fish meat. ANOVA results showed concentrations of 10% and 15% significant inhibition of the number of bacterial colonies as well as the storage time for 24 hours is the maximum length of time after treatment were compared with controls. Based on this, it can be concluded garlic extract is effective in inhibiting the growth of bacteria with the most effective concentration of 10% and maximum storage time is given 24 hours.
RESPON PERTUMBUHAN PLANLET ANGGREK Phalaenopsis amabilis L. var. Jawa Candiochid AKIBAT IRADIASI SINAR GAMMA
Life Science Vol 2 No 2 (2013)
Publisher : Universitas Negeri Semarang

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Penelitian ini bertujuan untuk mengetahui pengaruh iradiasi sinar gamma terhadap pertumbuhan planlet anggrek Phalaenopsis amabilis L. var. Jawa Candiochid. Penelitian ini adalah penelitian eksperimen dengan menggunakan Rancangan Acak Lengkap (RAL). Data dianalisis menggunakan anava satu jalan. Jika hasil uji Anava signifikan, maka akan dilakukan uji beda nyata terkecil (BNT). Hasil penelitian menunjukkan bahwa semakin tinggi dosis radiasi maka kecepatan munculnya daun dan akar semakin cepat tetapi pada jumlah daun dan akar semakin tinggi dosis radiasi maka semakin sedikit jumlah daun  dan akar yang tumbuh. Penelitian ini dapat disimpulkan bahwa pemberian iradiasi sinar gamma meyebabkan respon pertumbuhan yang bervariasi pada planlet anggrek Phalaeonopsis amabilis L. var. Jawa CandiochidThe research aimed to identify the influence of gamma-ray irradiation on the growth of Phalaenopsis amabilis L. var. Jawa Candiochid planlets. This was experimental research with completely randomized design. Data were analyzed by one way anova. If the result of anova is significant, it would be tested by Least Significant Difference (LSD). The result shows that addition gamma-ray irradiation with higher dosage ensued on rapidity of leaf appeartance rate and roots appeartancerate more rapidly but on leaves number and roots number less. The result shows that addition gamma-ray irradiation cause the different growth responses of Phalaenopsis amabilis L. var. Jawa Candiochid planlets.

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