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Hemera Zoa
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Biochemistry, Genetics & Molecular Biology
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Articles 250 Documents
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Search results for , issue "Proceedings of the 20th FAVA " : 250 Documents clear
VPH-6 Semi Quantitative Analysis of Rabies Entrance into Pisang Island from Pesisir Barat District of Lampung Tri Guntoro; . Putri; . Ferro
Hemera Zoa Proceedings of the 20th FAVA & the 15th KIVNAS PDHI 2018
Publisher : Hemera Zoa

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Abstract

Rabies is a zoonotic infectious animal disease. Genesis of rabies is feared among people because it almost ends in death. The disease is caused by the virusrabies, the genus Lyssavirus of the family Rhabdoviridae (Muleyaetal., 2012). The case of rabies / lyssa has long been recognized by the public and has been widespread in several countries of the world. Human deaths from rabies in Africa and Asia are estimated at 55,000 people per year (Knobel et al., 2005). The presence of rabies in Indonesia was first reported in the iPhones of West Java in 1884. To date, rabies in Indonesia still poses a major problem from the public health aspect with an average reported death rate of 125 people per year (Sedyaningsih, 2011). Therefore, rabies is grouped into strategic diseases and is given priority in its prevention, control and eradication.Pisang Island is located inPesisir Barat District which is one of the foreign tourist destinations. It is located very close to the west coastal district which has a high bite case (attached). It is therefore necessary to analyze the risk of rabies entry from the Pesisir Barat district to Pisang Island.
AQ-9 Identification of Sumateran Wild Boar Meat (Sus scrofa vittatus) by Restriction Fragment Length Polymorphism (RFLP) Analysis of Cytochrome b Gene Melani Wahyu Adiningsih; Retno Damayanti Soejoedono; Trioso Purnawarman; Hadri Latif; Rahmat Setya Adji; Okti Nadia Poetri; Dwi Desmiyeni Putri
Hemera Zoa Proceedings of the 20th FAVA & the 15th KIVNAS PDHI 2018
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Abstract

Sumateran wild boars have been super abundant in Sumateran forest. In Indonesia, this wildlife condition has led to the exploitation for commercial purpose. The high number of Sumateran wild boars population increases wild boar hunting resulting in an abundant availability of wild boar meat in the food market with extremely cheap price. The macroscopic similarity of wild boar meat and beef has prompted the local people to abuse this situation by selling wild boar meat in traditional market as beef. Based on annual record from Cilegon Class II Quarantine Office in 2014, there were nine smuggling cases or a total of 21.556 kg of wild boar meat smuggling effort that were prevented by Cilegon Quarantine officers. The number of food safety concerns related to smuggling of wild boar or counterfeiting beef with wild boar is a very detrimental condition for consumers, especially consumers in traditional markets.The checking of genuineness or validity of food products is an important effort to protect people from consuming unhealthy food and to indicate whether the food is halal or not. Studies of meat detection should be continuously developed as an effort to protect consumers. Genetic method is the most specific and sensitive method to check food ingredients authenticity by detecting the presence of genetic material or deoxyribonucleic acid (DNA). It results from the specific character of the structure of DNA particles and the possibility of using the information included in them. The most frequent loci used for species identificationin phylogenetics and biodiversity studies are mitochondrial cytochrome b (cyt b).Genetic method is the most specific and sensitive tool for analyzing the authenticity of food ingredients in a molecular level by means of detecting the presence of genetic material or deoxyribonucleic acid (DNA). One of the various methods could be used to detect genetic material is polymerase chain reaction (PCR). Specifically, one of such method frequently used in food industry to observe animal derived product fabrication is PCR restriction fragment length polymorphism (RFLP). PCR-RFLP is based on the comparison of the bands profile generated after certain enzymes digest the DNA target. PCR-RFLP is appropriate for meat testing due its ability in exploiting sequence variation in designated DNA region that allows species differentiation even from closely related species through DNA fragment restrictions selected by suitable restriction enzyme. PCR-RFLP is advantageous since it is simple, cheaper, and easier to be adjusted for routine big-scale studies such as surveillance program.
VPH-7 Study of Inappropriate Antibiotic Usage in Cattle Farm in East Nusa Tenggara Annytha Detha; Diana A Wuri; Filomena Ramos; Desi Biru; Margie Meha; Anita Lakapu
Hemera Zoa Proceedings of the 20th FAVA & the 15th KIVNAS PDHI 2018
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Abstract

Antibiotics have an inhibitory effect on the growth of microorganisms [1]. The inappropriate use of antibiotics has an impact on human health, animals and the environment [2,3]. Therefore prescription Antibiotic use should be given by veterinarian. In fact, a number of breeders are practicing antibiotic beef cattle without a veterinary prescription. The purpose of this study was to determine the percentage of antibiotics used without prescription from veterinarians by farmers in Kupang City, East Nusa Tenggara.
AQ-10 Potential Animal Carriers Rabies (HPR) Suspect Arriving from Java to Sumatera Basid Sastradiawan
Hemera Zoa Proceedings of the 20th FAVA & the 15th KIVNAS PDHI 2018
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Abstract

Rabies is zoonotic disease that is still haunting in severel in Indonesia. Management of rabies, both eradcation and vaccination continues. However, cultural constraints and lack of understanding and concern of all stakeholders are their own obstacles in handling and controlling this disease.The number of 102 titers of antibodies obtained in the HPR that were transversed from Java to Sumatera became an early warning, that the rabies control and eradication program on the island of Sumatera will experience difficulties.Support from all stakeholders to realize Sumatera free of rabies is no longer negotiable. The local government, police, Quarantine and community are the pioneers of success of the Sumatera-free rabies program. Without the synergy of the parties involved, the Sumatera-free Rabies program is just a dream.
AQ-11 Analysis Total Plate Count (TPC) Escherichia coli and Salmonella sp. on Frozen Beef Imported through Tanjung Priok Port G A Hanum; A Kurniawati; R Normaliska
Hemera Zoa Proceedings of the 20th FAVA & the 15th KIVNAS PDHI 2018
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Abstract

Beef is the third most consumed protein in Indonesia after chicken and fish. Beef is the most important source of protein used widely in a variety of traditional dishes, such as “bakso” (meatball) and “rendang” (meat dish cooked with coconut milk). Fresh meat products are been marketed either as freshly cooled or frozen.Foodborne pathogens such as bacteria or toxins, viruses or parasites may lead to human disease when contaminated food is eaten. The source of contamination may vary but harmful bacteria are mostly responsible for causing gastrointestinal infections. The sources could be the animal, the environment or contamination during food processing [1].                This study was carried out to evaluate the microbiological condition and status of frozen beef samples imported through Tanjung Priok port (Figure 1). The studied microbiological parameters were Total Plate Count (TPC), Escherichia coli,  and Salmonella, to determine it’s risk for public health.The amount of coliform bacteria in meat ex. E. coli is important. This microorganisms are very good indicators concerning whether the food has been processed in hygienic conditions. Salmonella bacteria is not exist in food.  The Salmonella on meat can be emphasized to be spread by the animals whose intestine, skin are cut and because of the equipment used in cutting and the unhygienic and careless working [4].
JSPS-1 Overview of Foot and Mouth Disease Control in Thailand and Southeast Asia Sith Premashthira
Hemera Zoa Proceedings of the 20th FAVA & the 15th KIVNAS PDHI 2018
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Abstract

Foot and mouth disease (FMD) is endemic in many countries of Africa, the Middle East and Asia, and FMD-free countries face greater risk of incursions due to increased global movement and trade of livestock and animal [1]. In the Southeast Asia and China (SEACFMD) roadmap, the framework supports member countries to consider and improve their Progressive Control Pathway (PCP-FMD) stages (0-5) of FMD control and applies the FMD Official Control Programme recognized by OIE (World Organisation for Animal Health).
AQ-12 Application of a Multiplex PCR Assay to Detect Campylobacter fetus subspecies venerealis from Imported Bovine Preputial Samples Mazdani Daulay; Adi Komara; Novera Nirmalasanti; Siti Khadijah; . Marjono; Melyna Sandra; Muhamad Taopik; . Mukromin; . Mustamil; Rahmat Setya Adji
Hemera Zoa Proceedings of the 20th FAVA & the 15th KIVNAS PDHI 2018
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Abstract

Campylobacteriosis, caused by Campylobacter spp., is of considerable economic importance to the cattle industry worldwide. Campylobacter spp. were recognized as etiological agents of abortion in sheep. Campylobacter fetus subsp. fetus (Cff) causes sporadic abortion in sheep, often late in gestation, while subspecies venerealis (Cfv) is a cause of sexually transmitted bovine infertility and sporadic abortion in cattle. Various investigations have been carried out in different countries to assess the prevalence and impact of this disease. Some published results surveys are outlined in Table 1. Table 1. A summary of published data showing the prevalence of C. fetus in different countriesStudyareaSample type(s)Sample sizePrevale nce ofC. fetus (%)Diagnostic methodAustralia(1985-1986) Bulls (preputi al suction)1 008animals41 herds87% herdspositiveSerological(Fluorescentantibody test) California(United States of America)Cows40047Serological(ELISA) New ZealandCows (vaginal mucous) and bulls (preputial wash)1 230 cows(125 herds)54 bulls70% herds positiveCfv : 0Cff/othersSerological(ELISA)Bacteriological culture          Cff: Campylobacter fetus subsp. fetus, Cfv: C. fetus subsp. venerealis. According to [1], Bovine Genital Campylobacteriosis (BGC) disease was classified as 1st Group of animal quarantine disease. It is an exotic disease that was not ever detected in Indonesia. However, large scale cattle importation to Indonesia from the countries which ever reported BGC prevalence in their territories, initiating and spreading BGC will be the major threat for feedlotter or dairy farm in Indonesia. Hence, we should apply diagnostic test to detect Cfv in order to prevent the introducing the BGC to Indonesia. The aim of this study was to verify that multiplex PCR assay applicative to detect Campylobacter fetus subsp. venerealis from field samples.
JSPS-2 Risk of FMD Importation Through Cattle Movement at the Thailand-Myanmar Border: A Preliminary Quantitative Risk Assessment Result Anuwat Wiratsudakul; Phrutsamon Wongnak; Terdsak Yano; Satoshi Sekiguchi
Hemera Zoa Proceedings of the 20th FAVA & the 15th KIVNAS PDHI 2018
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Abstract

Foot-and-mouth disease (FMD) is an important viral infection affecting productivity performance of many livestock species including cattle. Beef cattle are still traditionally raised in Thai households with small number of animals per family. However, a number of cattle are imported from neighboring countries, especially Myanmar. This importation is managed to supply the needs of domestic consumption and also to convey animals to the third countries.Tak, a Thailand-Myanmar border province, is a major gateway importing cattle from Myanmar. The animals imported into the province are then widely distributed to different regions of Thailand [1].  Therefore, FMD virus may move across the border and further disseminate across the country.The present study hence tried to comprehend the cattle importation process and employed risk assessment technique to quantify the risk of FMD importation via transboundary cattle movement.
JSPS-3 Detection of Pathogens Associated with Bovine Respiratory Disease: Clinical Cases in Thai Dairy Herds Kittisak Ajariyakhajorn; Thanasak Boonserm; Sukuma Samngamnim
Hemera Zoa Proceedings of the 20th FAVA & the 15th KIVNAS PDHI 2018
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Abstract

Bovine respiratory disease (BRD) is the major source of economic loss for the cattle industry. The major respiratory pathogens, such as bovine respiratory syncytial virus (BRSV), bovine viral diarrhea virus (BVDV), bovine herpesvirus type1 (IBR), Parainfluenza 3 (PI3) virus impaired the animal’s respiratory defenses in which Pasteurella multocida, Histophilus somni, Mannheimia haemolytica and Mycoplasma bovis caused the complex of respiratory disturbances. The investigation of clinical cases of BRD in dairy farms supports the information for control and treatment strategies. We aim to investigate pathogens that associated with clinical cases of bovine respiratory disease in Thai dairy herds.
AQ-13 A Cross Sectional Survey Seroprevalensi of Antibody Present Against Salmonella Pullorum from Chicken in Banjarmasin Quarantine Services Rima Hasmi Firdiati
Hemera Zoa Proceedings of the 20th FAVA & the 15th KIVNAS PDHI 2018
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Abstract

Chicken is a poultry that is widely maintained in Indonesian society. From the village even up to a small portion of the city's population. Chicken Benefits derived from chicken and others for, household, and meat for other relief. Maintenance of many chickens by the customs and religious activities. Bringing the exploration done by the people here is something that can be done both between regions and between islands. It causes chicken or poultry traffic is very high in The Agricultural Quarantine Service (BKP) Class I of Banjarmasin. Before carrier, chicken or poultry must be healthy from various diseases. Pullorum is one of the most important diseases in chickens. Pullorum is a Quarantine Animal Disease Pest Group II (HPHK Gol. II) Regulation of the Minister of Agriculture [1] on Classification of Types of Quarantine Animal Diseases, Classification and Carrier Media Classification.                Pullorum is a disease in chickens caused by Salmonella pullorum bacteria. Form of S. pullorum bacteria in the form of short stems, gram negative, not spora and chicken as its specific host. Penile transmission can occur vertically through eggs and mothers to their children or horizontally by direct or indirect contact. It can be directly through drinking water, feed, cage equipment and indirectly through vectors or intermediary animals such as insects and rats [2]. Chicks, adult chickens, peacocks, canaries, turkeys and ostriches. Pullorum infection in mammals is very rare despite research reports or natural infection in rabbits, pigs, cats, cattle and mice [3].                Symptoms of pullorum disease include white defecation and in young chickens or poultry causing very high mortality, while in adult chickens act as a career [2]. The purpose of this paper to know the sero prevalences of the   pullorum disease and factors associated with the incidence of S. pullorum in chickens carried in The Agricultural Quarantine Service (BKP) Class I of Banjarmasin.

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