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Trioso Purnawarman
Departemen Ilmu Penyakit Hewan Dan Kesehatan Masyarakat Veteriner Fakultas Kedokteran Hewan Institut Pertanian Bogor
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Computer Science & IT Decision Sciences, Operations Research & Management Economics, Econometrics & Finance Immunology & microbiology Medicine & Pharmacology Public Health Social Sciences Veterinary Other

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KEBERADAAN KOLIFORM PADA DAGING AYAM SUWIR BUBUR AYAM YANG DIJUAL DRAMAGA BOGOR Zelpina, Engki; Purnawarman, Trioso; Lukman, Denny Widaya
Jurnal Teknologi Pangan dan Gizi Vol 19, No 1 (2020)
Publisher : Widya Mandala Surabaya Catholic University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (448.4 KB) | DOI: 10.33508/jtpg.v19i1.2447

Abstract

Penelitian ini bertujuan untuk mengetahui cemaran bakteri koliform pada daging ayam suwir bubur ayam yang dijual Dramaga Bogor. Sampel daging ayam suwir bubur ayam yang digunakan berasal dari pedagang bubur ayam. Sejumlah 15 sampel daging ayam suwir bubur ayam di isolasi terhadap bakteri koliform. Metode yang digunakan adalah cara tuang (pour plate method). Data yang diperoleh dianalisis secara deskriptif. Hasil penelitian menunjukkan bahwa jumlah cemaran koliform pada daging ayam suwir bubur ayam yang dijual di Dramaga Bogor adalah 4,25 x 105 ± 2,74 x 105. Cfu/g. Jumlah koliform pada daging ayam suwir bubur ayam melebihi SNI sehingga dapat menimbulkan berbagai gangguan kesehatan masyarakat.
Deteksi Salmonella spp. pada Telur Ayam Konsumsi yang Dilalulintaskan melalui Pelabuhan Tenau Kupang Susanto Nugroho; Trioso Purnawarman; Agustin Indrawati
Acta VETERINARIA Indonesiana Vol. 3 No. 1 (2015): Januari 2015
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (226.83 KB) | DOI: 10.29244/avi.3.1.16-22

Abstract

Salmonelosis adalah salah satu penyakit food-borne bakterial zoonotik yang paling penting di seluruh dunia. Salmonella spp. adalah penyebab salmonelosis akibat konsumsi makanan berbahan dasar unggas dan produk unggas yang terkontaminasi. Unggas dan telur ayam dianggap merupakan salah satu reservoir Salmonella spp. yang paling penting. Salmonella spp. ditularkan melalui rantai makanan dan akhirnya menular ke manusia. Meningkatkan keamanan produk unggas dengan cara deteksi dini terhadap food-borne patogen merupakan komponen penting untuk membatasi kontaminasi Salmonella spp.. Metode deteksi dan identifikasi Salmonella spp. merupakan strategi yang dirancang untuk mencegah kontaminasi unggas dan produk unggas. Tujuan dari penelitian ini adalah untuk mendeteksi Salmonella spp. dari telur ayam yang berasal dari 4 pengirim telur menggunakan metode konvensional. Jumlah sampel dihitung dengan menggunakan rumus menduga prevalensi dan diambil menggunakan metode acak berlapis. Analisis data hasil positif Salmonella spp. dilakukan secara deskriptif. Berdasarkan hasil pengujian dari 270 sampel, 5 pengujian dengan metode konvensional positif Salmonella spp.. Berdasarkan hasil pengujian positif kontaminasi Salmonella spp. pada telur ayam maka diperlukan evaluasi terhadap pengiriman telur ayam konsumsi antar pulau.Kata kunci: kontaminasi, Salmonella spp., metode konvensional, telur ayam. (Detection of Salmonella spp. in Commercial Hen Eggs Entering through Tenau Port Kupang)Salmonellosis is one of the most important food-borne bacterial zoonotic diseases worldwide. Salmonella spp. are causative agent of salmonellosis associated with contaminated commercial poultry and poultry product. Poultry and eggs are considered one of the most important Salmonella spp. reservoirs. Salmonella spp. were able to pass through the food chain and ultimately transmitted to humans. Improving safety of poultry products by early detection of food-borne pathogens would be considered an important component for limiting exposure to Salmonella contamination. Detection and identification method for Salmonella spp. are considered to be an important component of strategies designed to prevent poultry and poultry product. The aims of the study were to detect Salmonella spp. from hen eggs collected from 4 exporters using conventional method. Samples size were calculated using estimates prevalence formula and selected by stratified random sampling. Data regarding the proportion of Salmonella spp. positive samples were analyzed descriptively. 270 samples, 5 test by conventional method were positive Salmonella spp.. According of positive test results Salmonella spp. contamination in hen eggs was necessary to evaluate the delivery of commercial hen eggs between islands.Keywords: conventional methods, hen eggs, Salmonella spp. contamination.
Trichinellosis pada Babi di Kota Manado Provinsi Sulawesi Utara Syahdu Pramono; Fadjar Satrija; Trioso Purnawarman
Acta VETERINARIA Indonesiana Vol. 4 No. 1 (2016): Januari 2016
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (530.403 KB) | DOI: 10.29244/avi.4.1.27-34

Abstract

Trichinellosis adalah penyakit zoonnotik yang berasal dari makanan yang disebabkan oleh cacing nematoda Trichinella spp. Penyakit ini masih kurang mendapatkan perhatian di negara maju dan negara berkembang. Parasit ini mempunyai distribusi yang sangat luas hampir di seluruh dunia. Tujuan dari penelitian ini adalah untuk mengkaji keberadaan trichinellosis pada daging babi di Manado. Penelitian ini menggunakan lintas sektional dengan total sampel otot maseter babi (n=139) dan otot diafragma babi (n=139) berasal dari 4 rumah potong hewan babi di Manado. Pengujian laboratorium terhadap Trichinella spp. menggunakan uji pool digesti terdeteksi larva yang diduga Trichinella spp. dalam satu pool yang terdiri dari 9 sampel otot diafragma babi. Pool yang terdeteksi positif kemudian secara individu diuji dengan menggunakan uji kompresi dan dilakukan pembuatan preparat dengan metode pengecatan Hemaktosili Eosin. Hasil dari pengujian individual tidak terdeteksinya Trichinella spp. pada sampel yang diuji. Berdasarkan hasil pengujian tersebut diatas daging babi yang diperiksa tidak terinfeksi oleh Trichinella spp.Kata kunci: digesti, kompresi, Trichinella spp. (Trichinellosis in Pig in Manado North Sulawesi Province)Trichinellosis is a food-borne zoonotic disease caused by the nematode Trichinella spp. However it is still a neglected disease in development and developing country. This parasite has worldwide distribution in a worldwide. The aims of this study were to observe the occurance Trichinella spp. in pork in Manado. The research was conducted using cross sectional study. A total of pig masseter muscle (n=139) and pig diaprhagmatic muscle (n=139) came from 4 slaughter house in Manado. Laboratory examination of Trichinella larvae using pooled sample digestion method was detected one larva in a pooled batch of 9 pig diaprhagmatic muscle samples whereas the suspected positive findings were individually subjected to the compression method and Hematoxilin Eosin staining method. The result showed that the infected  could not be identified. Based on these findings, the absence of Trichinella infection in pigs slaughtered pigs indicates that not infected by Trichinella spp.Keywords: compression, digestion, Trichinella spp.
Pengetahuan, Sikap, dan Praktik Peternak dalam Penggunaan Antibiotik pada Ayam Broiler di Kabupaten Subang Trioso Purnawarman; Rusman Efendi
Acta VETERINARIA Indonesiana Vol. 8 No. 3 (2020): November 2020
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/avi.8.3.48-55

Abstract

Masalah global yang dihadapi saat ini adalah tingginya penggunaan antibiotik yang tidak tepat. Penggunaan antibiotik yang tidak rasional pada manusia dan hewan mendorong pada kejadian resistansi antibiotik. Penelitian ini bertujuan untuk menganalisis pengetahuan, sikap, serta hubungannya dengan praktik penggunaan antibiotik pada peternak broiler. Metode penelitian menggunakan desain cross sectional. Sampel dalam penelitian ini sebanyak 74 peternak dari 126 peternak di Kabupaten Subang. Kriteria sampel peternak yang diambil memiliki broiler 5000-10000 ekor. Teknik pengambilan sampel dilakukan dengan cara stratified random sampling berdasarkan pola manajemen peternakan, rincian besar sampel peternakan ayam broiler adalah 71 peternak dengan pola manajemen inti kemitraan dan pola menajemen mandiri sebanyak 3 peternak. Pengambilan dari dua kelompok tersebut dilakukan secara acak. Data diambil menggunakan kuesioner dengan cara wawancara, kemudian dianalisis dengan uji chi-square dengan tingkat kemaknaan α ≤ 0.05. Hasil penelitian menemukan sebagian besar peternak memiliki pengetahuan yang sedang tentang penggunaan antibiotik pada broiler. Peternak yang memiliki sikap baik dalam penggunaan antibiotik berjumlah berjumlah 7%, paling sedikit bila dibandingkan dengan yang sikapnya sedang sebanyak 76% dan kurang sebanyak 17%. Praktik peternak dalam menggunakan antibiotik pada broiler sebagian besar termasuk dalam kategori sedang. Terdapat hubungan yang bermakna antara pengetahuan dengan sikap peternak dalam penggunaan antibiotik pada broiler. Terdapat hubungan yang bermakna antara pengetahuan dengan praktik peternak dalam menggunakan antibiotik pada broiler dengan nilai p=0.028 ≤ 0.05. Sikap tidak dengan praktik penggunaan antibiotik pada broiler. Kesimpulannya adalah pengetahuan berhubungan dengan praktik peternak dalam penggunaan antibiotik pada broiler.
AQ-9 Identification of Sumateran Wild Boar Meat (Sus scrofa vittatus) by Restriction Fragment Length Polymorphism (RFLP) Analysis of Cytochrome b Gene Melani Wahyu Adiningsih; Retno Damayanti Soejoedono; Trioso Purnawarman; Hadri Latif; Rahmat Setya Adji; Okti Nadia Poetri; Dwi Desmiyeni Putri
Hemera Zoa Proceedings of the 20th FAVA & the 15th KIVNAS PDHI 2018
Publisher : Hemera Zoa

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (561.42 KB)

Abstract

Sumateran wild boars have been super abundant in Sumateran forest. In Indonesia, this wildlife condition has led to the exploitation for commercial purpose. The high number of Sumateran wild boars population increases wild boar hunting resulting in an abundant availability of wild boar meat in the food market with extremely cheap price. The macroscopic similarity of wild boar meat and beef has prompted the local people to abuse this situation by selling wild boar meat in traditional market as beef. Based on annual record from Cilegon Class II Quarantine Office in 2014, there were nine smuggling cases or a total of 21.556 kg of wild boar meat smuggling effort that were prevented by Cilegon Quarantine officers. The number of food safety concerns related to smuggling of wild boar or counterfeiting beef with wild boar is a very detrimental condition for consumers, especially consumers in traditional markets.The checking of genuineness or validity of food products is an important effort to protect people from consuming unhealthy food and to indicate whether the food is halal or not. Studies of meat detection should be continuously developed as an effort to protect consumers. Genetic method is the most specific and sensitive method to check food ingredients authenticity by detecting the presence of genetic material or deoxyribonucleic acid (DNA). It results from the specific character of the structure of DNA particles and the possibility of using the information included in them. The most frequent loci used for species identificationin phylogenetics and biodiversity studies are mitochondrial cytochrome b (cyt b).Genetic method is the most specific and sensitive tool for analyzing the authenticity of food ingredients in a molecular level by means of detecting the presence of genetic material or deoxyribonucleic acid (DNA). One of the various methods could be used to detect genetic material is polymerase chain reaction (PCR). Specifically, one of such method frequently used in food industry to observe animal derived product fabrication is PCR restriction fragment length polymorphism (RFLP). PCR-RFLP is based on the comparison of the bands profile generated after certain enzymes digest the DNA target. PCR-RFLP is appropriate for meat testing due its ability in exploiting sequence variation in designated DNA region that allows species differentiation even from closely related species through DNA fragment restrictions selected by suitable restriction enzyme. PCR-RFLP is advantageous since it is simple, cheaper, and easier to be adjusted for routine big-scale studies such as surveillance program.
KEBERADAAN Salmonella sp. PADA DAGING AYAM SUWIR BUBUR AYAM YANG DIJUAL DI LINGKAR KAMPUS INSTITUT PERTANIAN BOGOR DRAMAGA BOGOR Engki Zelpina; Trioso Purnawarman; Denny Widaya Lukman
Jurnal Penelitian Pascapanen Pertanian Vol 15, No 2 (2018): Jurnal Penelitian Pascapanen Pertanian
Publisher : Balai Besar Penelitian dan Pengembangan Pascapanen Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jpasca.v15n2.2018.73-79

Abstract

Penelitian ini bertujuan untuk melakukan studi mengetahui keberadaan Salmonella sp. pada daging ayam suwir bubur serta rekomendasi agar aman dan layak untuk dikonsumsi. Penelitian ini merupakan kajian lapang cross sectional study. Populasi dalam penelitian ini adalah seluruh pedagang bubur ayam yang berada (radius 100 meter) di lingkar kampus Institut Pertanian Bogor, Dramaga, Bogor. Penelitian dilakukan melalui wawancara, observasi, dan pengujian laboratorium. Sampel pada penelitian ini diambil dari 15 pedagang bubur ayam, setiap pedagang diambil sampel sebanyak 3 kali ulangan, total sampel adalah 45. Analisis data dilakukan secara univariat dan bivariat (fisher exact test). Hasil penelitian menunjukkan bahwa ditemukan keberadaan Salmonella enteretica serovars Enteritidis dalam daging ayam suwir bubur ayam sebanyak 6,66% (3/45) dan terdapat hubungan antara asal daging ayam dan keberadaan Salmonella Enteritidis. (p value=0,022 dan CC=0,577). This study was aims to conducted studies to determine the presence of Salmonella sp. in shredded chicken meat of chicken porridge and recommendations for safe for consumption. A cross sectional study approach was used in this study. The population in this study was all chicken porridge seller which located in radius of 100 m around of the campus area Bogor Agricultural University, Dramaga, Bogor. The research was done through interview, observation and laboratory examination. The samples in this study were 15 chicken porridge seller with repeated for three times and total number of samples were 45. The data were analyzed by univariat and bivariate (fisher exact test). The results of this study showed the presence of Salmonella Enteritidis in 6.66% (3/45) of shredded chicken meat chicken porridge and there was a relationship between the origin of chicken meat and the presence of Salmonella Enteritidis (p value = 0.022 and CC = 0.577).
Identifikasi Listeria monocytogenes pada Susu Kambing di Kabupaten Purworejo Jawa Tengah Monika Danaparamitha Andriani; Trioso Purnawarman; Retno Damayanti; Syafril Daulay
Jurnal Sain Veteriner Vol 34, No 1 (2016): Juni
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (989.878 KB) | DOI: 10.22146/jsv.22809

Abstract

Listeria monocytogenes is a pathogenic Listeria species, especially for high-risk groups and it that can be transmitted through contaminated food. Goat milk produced by traditional milking process and storage has a high risk of contamination. The aim of this study was to identify the presence of L. monocytogenes in fresh goat milk in Puworejo regency, Central Java. This study used 60 samples of raw goat milk that were obtained from seven farms by disease detection sampling method. All of the used method in this research refer to Indonesian National Standard (SNI) ISO 11290-1: 2012 about Microbiology of food and feed for detection and enumeration of Listeria monocytogenes. A total of 60 samples of raw goat milk that used in this study were not contaminatedwith L. monocytogenes. Based on the results of this study, it can be concluded that all the samples of raw goat milk were free from L. monocytogenes and have fulfilled the Indonesian National Standard (SNI) ISO No. 7388: 2009 about Limit of Microbial Contamination in Food.
Prevalence of Salmonella Spp. Bacteria Antibiotic Resistance in Digestion Tract in the Broiler Farms of Subang District Septa Walyani; Trioso Purnawarman; Etih Sudarnika
Buletin Peternakan Vol 43, No 1 (2019): BULETIN PETERNAKAN VOL. 43 (1) FEBRUARY 2019
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v43i1.41010

Abstract

This study is aimed to  estimate the prevalence of resistant Salmonella spp., determine the spread of bacterial resistance and investigate the serotypes of bacteria in the chicken’s digestion tract in the broiler farms in Subang District. As many as 74 farms were chosen, five poled caeca samples were taken from each farm and tested for isolation and identification of Salmonella spp. Salmonella isolates obtained were tested antimicrobial susceptibility against 8 antibiotics using the agar dilution method. The antibiotics were gentamycin, tetracycline, ciprofloxacin, nalidixic acid, ampicillin, chloramphenicol, trimethoprim, and sulfamethoxazole. The result showed that 8 out of 74 samples were positive for Salmonella. The prevalence of Salmonella spp. in the digestion tract was 10.8%; 95% confidence interval 3.7%-17.9%. Based on the serological test eight serotypes obtained were Salmonella enteritidis, Salmonella oslo, Salmonella narashino, Salmonella nakuru, and Salmonella nordufer. The result of antibiotic resistance test showed that from 8 Salmonella isolates obtained, 12.5% were found to be sensitive, 75% isolates were resistant to one or two antibiotics, and the remaining 12.5% isolates were resistant to more than two antibiotics; 95% confidence interval (0%-35.4%). The prevalence of resistant Salmonella spp. bacteria in chicken digestion tract in broiler farms in Subang District was high, so integrated control program to reduce antimicrobial resistance problem in broiler farm are greatly needed.
ANALISIS PENDUKUNG KEPUTUSAN PENILAIAN PRESTASI KERJA BERDASARKAN SASARAN KERJA DAN PERILAKU KERJA TENAGA KEPENDIDIKAN Maulita Lutfiani; Mohamad Syamsul Maarif; Trioso Purnawarman
MIX: JURNAL ILMIAH MANAJEMEN Vol 9, No 1 (2019): MIX: Jurnal Ilmiah Manajemen
Publisher : Universitas Mercu Buana

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (490.289 KB) | DOI: 10.22441/mix.2019.v9i1.002

Abstract

This study aimed to 1) Analyze the implementation of employee performance target (SKP) on the assessment of the performance appraisal of educational personnel in the the Faculty of Veterinary Medicine, Bogor Agricultural University (FKH-IPB). 2) Analyze the application of work behavior to the assessment of the performance appraisal of educational personnel in FKH-IPB. 3) Formulate strategic recommendations to be made by the FKH-IPB to improve assessment of performance appraisal and work productivity of educational personnel. The scope of this research is the assessment of the performance appraisal of education personnel, particularly regarding the application of the SKP and work behavior element as an civil servant (ASN) performance appraisal system. This study used the Analytical Hierarchy Process (AHP) method by distributing questionnaires and interviews to 12 experts. The results showed that 1) Creativity and innovation need to be added in the assessment of SKP in FKH-IPB to increase the productivity of education staff the weight of SKP becomes 65.4%. 2) Improved assessment of work behavior is very good by conducting guidance and supervision of education personnel periodically through the commitment and discipline of education staff. 3) The most influential performance improvement strategy is through education.
Pengaruh Waktu Penyimpanan Ekstrak Rennet Abomasum Domba Lokal terhadap Kualitas Keju Trioso Purnawarman; Chairun Nisa; Karunia Maghfiroh
Jurnal Sains Terapan : Wahana Informasi dan Alih Teknologi Pertanian Vol. 2 No. 1 (2012): Jurnal Sains Terapan, Volume 2, Nomor 1, Tahun 2012
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (433.107 KB) | DOI: 10.29244/jstsv.2.1.30-38

Abstract

The study was aimed to examine the storage effect of rennet extracted from abomasum of local sheep on the texture and organoleptic qualities of cheese including color, flavor, salty taste, and bitter taste. Ten samples of crude rennet extract were divided into two groups which each five samples were stored for 2 weeks and 24 weeks  respectively. The rennet extract was then used as milk clotting agent in cheese making process. Streptococcus thermophilus and Lactobacillus bulgaricus were used as starter in those processes. The fresh cheese was coated with beeswax and ripened for 20 days in refrigerator. The ripened cheese was analyzed for the quality. The texture was analyzed by using warner blatzer shear (INSTRON®) and the data were evaluated with completely randomized design (CRD). The result showed that both time storage of rennet extract were not significantly influence on cheese texture (P<0,05). The organoleptic tests of color, flavor, salty taste, and bitter taste were rated by 25 panelists and then the data were analyzed with Friedman test. The time storage of rennet extract resulted variation on organoleptic quality of cheese.  Descriptive statistical analyses provided information that each panelist has varied preferences on cheese samples. It can be concluded that the storage of rennet extract had no significantly influence on the texture of cheese and provided variation  on organoleptic test. Keywords: storage, rennet extract, cheese, organoleptic test
Co-Authors A.S. Satyaningtijas Afiff , Usamah Agus Setiyono Agustin Indrawati Aji Barbora Niasono Amalo, Gabryella Fransina Anisa Rahma, Anisa annisa, rizka fajri Aulia Andi Mustika Ayunina, Yasmine Qurrota Chaerul Basri Chairun Nisa Connie Januari Debby Fadhilah Pazra Denny Widaya Lukman Dwi Desmiyeni Putri Dwida Agustina Suherman Engki Zelpina Etih Sudarnika Fachriyan Hasmi Pasaribu Farrah Nadhira, Ivania Farrah Nadhira Gabryella Fransina Amalo Hadiyanti, Cresensia Rara Hadri Latif Hasudungan A Sidabalok Hasudungan A Sidabalok, Hasudungan A Herwin Pisestyani I wayan Teguh Wibawan IETJE WIENTARSIH Joko Pamungkas Karunia Maghfiroh Khoirun Nisa Kumala Andri Asari Lina Noviyanti Sutardi Madyastuti, Rini Maulita Lutfiani Melani Wahyu Adiningsih Miftahurridho, Zoelva Mohamad Syamsul Ma’arif Monika Danaparamitha Andriani Muharam Saepulloh Nugroho, Radhitya Aryo Okti Nadia Poetri Rahmat Setya Adji Retno Damayanti Soejoedono Rusman Efendi Santosa, Alfian Sa’diah, Siti Septa Walyani Sudarwanto, Mirnawati Bachrum Surachmi Setiyaningsih Susanto Nugroho Syafril Daulay Syahdu Pramono Tarigan, Ronald Vindriati, Zukhrufa Vista Whardani, Vivien Kusuma Yasmine Qurrota Ayunina