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INDONESIA
Hemera Zoa
Published by Institut Pertanian Bogor
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Core Subject : Science,
Biochemistry, Genetics & Molecular Biology
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Articles 391 Documents
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Kajian morfologi spermatozoa sapi simmetal di beberapa Balai Inseminasi buatan Indonesia M.Riyadhi .; R.I. Arifiantini .; B.Purwantara .
Hemera Zoa Vol. 1 No. 2 (2010): Jurnal Hemera Zoa
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Abstract

Morphological abnormality of sperm in semen of males have been associated with sub fertility and sterility for many years. This study to evaluated spermatozoa morphology especially primary abnormality of Simmental bull spermatozoa at several Artificial Insemination centre in Indonesia. One ejaculated were examined from each bull. Total of 70 bulls were tested in this study. One drop of semen was placed on each of 3-4 glass slides, and smears were prepared and air-dried. The spermatozoa were stained with carbolfluchsin-eosin (Williams stained). The types of morphological abnormalities were counted from 500 cells. Result demonstrated that from 70 bulls samples, 48 bulls (68.6%) were indicated low sperm abnormality (10%) were found in 6 bulls (8.57%). The highest type of abnormality found in all samples was pearshape (2.81±0.36%) and lowest were macrocephalus and double head (0.01±0.01%)
An evaluation of grati bull fertility in Pujon East Java A. Winanta
Hemera Zoa Vol. 74 No. 1 (1991): Jurnal Hemera Zoa
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A total of 258 measurements of scrotal circumference (SC) was made on 53 Grati bulls in Pujon, East Java, from June 1981 unti June 1985. Body weight (BW) and age of bulls ranged from 110 to 393 kg and from 12 to 65 months, respectively. The cummulative BW increased rapidly until about 32 months of age and there after slowly to a maximum of 393 kg at 54 monts age. The curviliear regression to fit BW data was Y = -25.83+14.27x - 0.13X2 where Y=BW in kg and X=age in months (r2=0.91).
Tingkat pertumbuhan dan analisa protein sel-sel fibroblas fetal tikus hasil kultur in vitro I.Djuwita .; Harlystiarini .; T. Widyaputri .; A. Efendi .; E.M Kaiin .; Nurhidayat .
Hemera Zoa Vol. 1 No. 2 (2010): Jurnal Hemera Zoa
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Research has been conducted on in vitro growth of rat fetal fibroblast cells in Dulbecco S Modzfied Eagle Medium (DMEM) containing 10% Fetal Bovine Serum (FBS) and 50 µg/ml gentamycin. Culture was done in 5% CO2 incubator at 37°C for 10 days. Evaluation was done on the proliferation rate and the protein production after several passages. The growth of the fibroblast was confirmed by their morphology. The proliferation rate was done based on the population doubling time (PDT) by counting the number of cells in each passage by using Improved Neubauer hemocytometer. Protein secreted into the culture medium without FBS, designed as conditioned medium of rat fetal fibroblast (CM-RFF) was analysized using sodium dodecyl sulfatepolyacrilamidegel electrophoresis (SDS-PAGE) and biological assay on its potency to inhibit differentiation of bone marrow mesenchymal stem cells (BMSCs). Quantitative data were analyzed using statistical T-test on Minitab program. The results showed that based on the population doubling time, the proliferation rate was increased in line with the number of passages and basedon the SDP-PAGE analysis, the fibroblast cells secreted several protein included in estimation a 34 kDa protein that can be maintained until 9 passages. Addition of 25% CM-RFF into mDMEM (without FBS) did not increase the percentage of bone marrow mesenchymal stem cells-like, but signiJicantly increased the percentage of fibroblast. The presenced of LIF in the medium significantly increased the percentage of BMSCs-like and fibroblast. It can be concluded that thegrowth and protein secretion of rat fetal fibroblast cells can be maintain until 9 passages; but the secreted protein could not increased the percentage of the bone marrow mesenchymal stem cells like.
Bursitis infeksiosa pada ayam ras dan bukan ras R R Jajuk Achdijati
Hemera Zoa Vol. 74 No. 1 (1991): Jurnal Hemera Zoa
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Selama periode Juli sampai Oktober 1987, lebih dari duaribu ayam ras dan bukan ras (buras) berumur 14-30 hari dari berbagai lokasi peternakan ayam di kabupaten Jayapura dilaporkan mati. Gejala klinik yang dilaporkan berupa depresi, tidak mau makan dan mencret. Pemeriksaan patologik dilakukan terhadap 12 spesimen, berupa 9 ekor ayam sakit dan 3 kadever dan ditemukan gejala utama berupa perbarahan dan pembengkakan oedematous dari bursa Fabricius. Disamping itu, karkas ayam memperlihatkan dehidrasi, ptechie pada kaki dan sayap serta pembengkakan ginjal dengan bercak-bercak putih. Penyakit ini diduga sebagai Bursitis Infeksiosa yang disebabkan  oleh virus RNA dan umumnya menyerang anak ayam berumur 3-6 minggu dengan angka kematian sektar 40%.
Growth and sexual development of javanese thin-tailed ewe lambs as affected by moderate internal parasite burden I. K. Sutama
Hemera Zoa Vol. 74 No. 1 (1991): Jurnal Hemera Zoa
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The effect of a moderate burden of internal parasites on growth and sexual development of Javanese thin-tailed (JIT) ewe lambs was studied from weaning at 13 weeks of age to puberty (first oestrus group (T) was infected with third stage mixed larvae comprised of 840 Haemonchus spp., 150 Cooperia spp., 255 Oesophagostomum spp., 240 Tri chostrongylus spp., and 15 Strongyloides spp. The lambs were fed elephant grass and concentrate (Beef0kwik, Cargill) ad lib. throught the experiment period. The result showed that a moderate parasite burden did not significantly influence growth and sexual development of JTT ewe lambs reared under research station conditions. However, group T ewe lambs consumed 8% more feed than group C. The possibility that JTT sheep may have a high resistance to internal parasite warrants further investigation.
Identifikasi infeksi Koi Herpes Virus (KHV) pada ikan Koi (Cyprinus carpio) dengan metode Polymerase Chain Reaction (PCR), imunositokimia dan imunohistokimia S. Edi .; O. Surfianti .; N. Christy .; R. Wiis .; Laminem .; E.R. Ekoputri .; M. Fathoni .; A. D. Koswara .; Nurhaidin .; U. Yanuhar .
Hemera Zoa Vol. 1 No. 2 (2010): Jurnal Hemera Zoa
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Abstract

Koi herpes vivus (KHV) disease has been diagnosed in koi and goldfish, and KHV is believed to remain in the body of its host to survive so that goldfish have the potential us a carrier virus. The purpose of this test is to look for another alternative method in identzfiing a particular fish disease Koi Herpes Virus (KHV) that is more simple and practical, rapid, precise and accurate as well as the examination results are expected to match the other examination techniques. Sample testing activities are positively infected with KHV derivedfrom Blitar marked by the characteristics of the most visible of the lesions (injuries) is congestion, bleeding onjns or body, or hemorrhagic on the basis of dorsal fins, pectoral fin, and anal Jins and the operculum, necrosis and nodules (nodule) in the gills white. The result of the testing activities identijcation of KHV by PCR, IHC and ICC were by using PCR samples obtained 7 positive KHV with DNA quality between 1.83 to 1.98 and DNA quantity between 31 7.89 to 492.08 at 290 bp DNA bands. It can be concluded that the alternative examination or identification of infections of Koi Herpes Virus (KHV) in addition to the koifish using PCR, immunohistochemistry, also can be developed using immunocytochemistry method (Streptavidin Biotin) due to simpler procedures, work more practical, lower cost, faster time, precise and accurate target.  
Isolation, serotyping, antimicrobial minimum inhibitory concentration and phatogeneticity determination of erysipelothrixrhusiopathiae from tonsils of apparently healthy slaughter pigs. Kamaluddin Zarkasie; Toshio Takahashi; Siti Mariana; Sumadi .
Hemera Zoa Vol. 74 No. 1 (1991): Jurnal Hemera Zoa
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Erysipolothri rhusiopathiae was isolated from tonsils of 245 (37.5%) of 687 appparently healthy slaughter pigs. Serotyping was performed by an agar gel double diffusion test. Of these 245 isoates, 95 (38.8%) were serotype N, 58 (23.7%) serotype 2, 18 (7.3%) serotype 11, 13 (5.3%) serotype 12, and the remaining 61 isolates were sterotypes 1a, 3, 4, 5, 6, 8, 9, 10, 13, 19 and 22. Antimicrobial Minimum Inhibitiory Concentration (MIC) was determined by two-fold agar dilution test. All isolates were highly suspectible to penicillin G, ampicillin and crythromycin and moderately susceptible to olean domycin, oxytetracyline, chloramphenicol and dihydrostreptomycin and moderately suspectible to oleandomicin, oxytetracyline, chloramphenicol and dihydrostreptomycin. Sulfadimethoxine showed no activity against the isolates. Pathogenicity test showed highly virulent for mica (LD5 0 < 10 3-0 CFU) whereas serotypes 19 and 22 were non-virulent.
Pertumbuhan histologik rumen dan retikulum domba Lubis Darmin
Hemera Zoa Vol. 70 No. 1 (1978): Jurnal Hemera Zoa
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Empat spesimen prenatal dan dua spesimen pascanatal berupa rumen dan retikulum domba dengan kisaran panjang c-r dan umur dari 3.5 em (atau kira-kira umur kandungan 39 hari) sampai umur 3 bulan telah diperiksa secara histologik dan histokimia. Pengamatan terperinci dilakukan terhadap perkembangan epitelnya. Epitel rumen dan retikulum amat tebal pada stadium prenatal dan mencapai ketebalan maksimal pada akhir masa bunting. Tetapi pada stadium awal pascanatal epitel menjadi lebih tipis. Epitel kolumnar berlapis banyak pada awal masa bunting berubah menjadi tipe pipih berlapis banyak dalam stadium bunting lebih tua. Ciri-ciri utama dari lapisan-lapisan epitel umur dan retikulum telah ada pada waktu lahir dan struktur dewasa yang normal dicapai antara waktu lahir dan umur 3 bulan. Epitel dan muskularis eksterna rumen dan retikulum tumbuh sendiri-sendiri. Permukaan selaput lendir (mukosa) rumen dan retikulum mempunyai banyak lipatan atau papila-papila termasuk papila-papila kerucut dan badan papila (papillary bodies). Perkembangan lipatan-lipatan terjadi sebagai suatu proses evaginasi epitel oleh pusat-pusat tenunan pengikat yang disusul dengan pembentukan invaginasi-invaginasi pada permukaan paling luar epitel. Penyimpanan dan penyebaran glikogen di dalam epitel rumen dan retikulum yang sangat menonjol pada awal stadium pranatal, menjadi sangat berkurang pada awal stadium pascanatal, dan hampir hilang dari epitel pada waktu lahir. Dapat diduga bahwa glikogen epitel ikut serta dalam metabolisme sel lokal untuk menyediakan tenaga bagi pertumbuhan dan pemeliharaan sel-sel epitel
Preparasi antigen KHV untuk pencegahan infeksi KHV pada ikan koi (Cyprinus carpio) E. Sulistiyowati .; St.S. Yasin .; W. Suharni .; S.R. Setyaningsih .; U. S Kuba .; Saribanong .; Hasmi .; St. Narwiyani .; Suriati .; Widodo .
Hemera Zoa Vol. 1 No. 2 (2010): Jurnal Hemera Zoa
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Abstract

Infeksi Koi Herpes Virus merupakan penyakit sangat serius yang menyerang ikan mas dan koi. Penyakit ini menyebabkan kematian massal sebesar 80-95% dari total populasi dan menimbulkan kerugian ekonomi yang sangat besar. Tujuan dari studi ini adalah untuk mendapatkan antigen HlV yang dapat digunakan untuk mencegah infeksi KHV. Metode preparasi antigen dilalakukan dengan membuat suspensi organ 10 % dari seluruh organ target ikan yang positif terinfeksi KHV, kemudian dipekatkan sehingga menghasilkan konsentrat organ (antigen). Sebagian dari konsentrat organ (antigen) yang dihasilkan, di-inaktif-kan dengan formaldehyde 0,3%, Kemudian disuntikkan pada ikan koi sehat (vaksinasi). Penyuntikan dilakukan sebanyak 3 kali dalam kurun waktu 4 minggu. Satu minggu setelah penyuntikan ke-3 dilakukan uji tantang. uji tantang dilakukan dengan menyuntikan virus aktif KHV dengan dosis 0,1 ml secara intraperitoneal pada koi yang telah divaksin. Sebagai kontrol, dilakukan juga penyuntikan pada koi yang tidak diberi perlakuan. Hasil uji tantang menunjukkan bahwa semua ikan yang divaksinasi dengan ketiga metode preparasi antigen tidak menunjukkan gejala klinis terinvaksi KHV.
Restriction Fragment Length Polymorphism (RFLP) gen sitokrom b dari delapan species burung D.A. Wibowo .; W.E. Prasetyaningtyas .; I. Djuwita .
Hemera Zoa Vol. 1 No. 2 (2010): Jurnal Hemera Zoa
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The aim of this research is to study the Restriction Fragment Length Polyorphism (RFLP) of eight species local birds of the cytochrome b mitochondrial DNA fiagment. The results gainedfiom this study is expected to be used as basic information for tissue birds species recognition. Eight bird I I tissues collected from Bogor area were preserved in 4,5 M NaCI containing 25% DMSO. DNA were isolated using ammonium acetat precipitation. Universal oligonucleotide cytochrome b primers L14841/H15149 were used for amplification of the mitochondrial cytochrome b DNA fragment using Polymerase Chain Reaction (PCR) method, followed by determination of the PCR product by electrophoresis. The amplicon were digested using restriction enzymes Hinf I (2,5 unit) and Rsa I (20 unit) for 2 and 6 hours, respectively. The calculation of the DNA amplicon and restriction fragment is done by measuring the distance between sample DNA migration with the DNA ladder in 2% agarose gel. The results showed that eight local birds species have 359 bp amplicon, and have different Hinf I restriction fragment size, except between scaly-breasted munia and streaked weaver, and also have difflerent Rsa I restriction fragment size except streaked weaver and javan turtle dove, and also chicken and yellow-vented bulbul. In conclusion, universal oligonucleotide cytochrome b primer followed by digestion with Hinf I and Rsa I restriction enzymes can be used for the eight birds species recognition.  

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