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Stigma : Jurnal Matematika dan Ilmu Pengetahuan Alam Unipa
Published by Universitas PGRI Adi Buana Surabaya
ISSN : 14121840     EISSN : 26219093     DOI : https://doi.org/10.36456/stigma.15.01
Core Subject : Education,
Mathematics
Jurnal STIGMA adalah jurnal ilmiah Biologi dan Biologi Terapan yang memuat artikel-artikel ilmiah. Jurnal STIGMA diterbitkan oleh Program Studi Biologi-Fakultas Matematika dan Ilmu Pengetahuan Alam - Universitas PGRI Adi Buana Surabaya. Terbit dua kali dalam satu tahun (April-Juli dan September-Desember), Jurnal ini memfasilitasi penelitian dengan tema Biologi Umum, Biologi Terapan, Bioteknologi, Aplikasi Ilmu Biologi.
Arjuna Subject : Umum - Umum
Articles 10 Documents
 1 
Search results for , issue "Vol 10 No 02 (2017)" : 10 Documents clear
Pemberian Ekstrak Biji Alpukat (Persea americana Mill.) dan Biji Sirsak (Annona muricata L.) terhadap Mortalitas Larva Nyamuk Culex Sp S. Nombe; Diah Karunia Binawati
STIGMA: Jurnal Matematika dan Ilmu Pengetahuan Alam Unipa Vol 10 No 02 (2017)
Publisher : FMIPA : Universitas PGRI Adi Buana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36456/stigma.vol10.no2.a1027

Abstract

Vegetable insecticide is a natural material that is environmentally friendly. This material can be used in the prevention and control of mosquito larvae, especially Culex sp. Culex sp is the main vector of filariasis disease and dengue hemorrhagic fever (DHF). Use of natural materials is very good and environment friendly as insectcide compared with chemicals. Avocado seed extract (Persea americana Mill) is known to have secondary metabolite content of alkaloids, saponins, triterpenoids while soursop extract (Annona muricata L) in the form of acetogenin, squamocin and annonacin. This research needs to use of avocado seed extract (P. americana Mill) and soursop seed extract (A. muricata L) as larvacide of Culex sp. The aim of this research is to know the effect of avocado seed extract (P. americana Mill) and soursop seed extract (A. muricata L) to mortality of Culex sp Instar III mosquito larvae and LC50 for 24 hours. This study was an experimental study using a complete randomized design (RAL) with five treatment concentrations (0, 2, 4, 6, and 8%) avocado seed extract (P. americana Mill) and soursop extracts extract (A. muricata L) as much as five repetitions. The data obtained were analyzed by using One Way ANOVA to show the effect on the treatment. The results showed that giving of avocado seed extract (P. americana Mill) with 8% concentration (23,8 ± 0,45 larvae) was optimal in killing Culex sp mosquito larvae. While giving soursop seed extract (A. muricata L) with 4% concentration (25 ± 0,0 larva) optimal in killing Culex sp mosquito larvae. Avocado seed extract (P. americana Mill) has an effect as a larvacide against Culex sp. instar III with LC50 at concentration 2,486% while soursop extract (A. muricata L) with LC50 value at concentration 1,605%. Keywords: Larvacide, soursop seed extract, avocado seed extract, Culex sp
UJI BANDING LIMBAH CAIR IKAN, AZOLLA DAN LIMBAH KULIT PISANG SEBAGAI NUTRISI TAMBAHAN UNTUK KUALITAS PRODUKSI TANAMAN CABAI RAWIT (Capsicum frutencens L.) N. F. Rochmana; Ngadiani Ngadiani
STIGMA: Jurnal Matematika dan Ilmu Pengetahuan Alam Unipa Vol 10 No 02 (2017)
Publisher : FMIPA : Universitas PGRI Adi Buana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36456/stigma.vol10.no2.a1028

Abstract

Household waste in the form of fish waste, azolla pinnata, and banana peel waste is a waste that has not been utilized optimally. The potential waste can be utilized as a raw material of organic nutrients with macro elements more complete than an-organic nutrients. Giving various variations of organic liquid nutrients can be used to chili (Capsicum frutencens L.). This study aims to determine the effect of different variations of organic liquid nutrient on the quality of chili production (Capsicum frutencens L.). The type of this research is quantitative research with experimental method using Completely Randomized Design consisting of 3 treatments 6 replications, so that there are 18 experimental units adn placed randomly was fish waste 20% (N1), 20% Azolla Pinnata (N2), and banana peel waste 20% (N3). Observations were made on three parameters of production quality is colour, taste, and wet weight. Technical of data analysis using One-way ANOVA with significance level of 0,05. One-way ANOVA test results on the quality of chili production is P>0,05. One-way ANOVA test results showed no signification on the quality of chili production (Capsicum frutencens L.). Keywords: Organic Liquid Nutrition, Liquid Waste Fish, Azolla Pinnata, Banana Peel Waste, Quality Productions of Plants, Chili.
PENGARUH ELISITOR Al3+ TERHADAP AKUMULASI DAIDZEIN DAN GENISTEIN PADA KALUS Phaseolus vulgaris L. Dan Phaseolus aureus Roxb. S. K. Dewi; W. Widoretno; Warsito Warsito
STIGMA: Jurnal Matematika dan Ilmu Pengetahuan Alam Unipa Vol 10 No 02 (2017)
Publisher : FMIPA : Universitas PGRI Adi Buana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36456/stigma.vol10.no2.a1029

Abstract

The Estrogen was very beneficial hormone for men and women. Isoflavon compound was natural estrogen source which contain three main compound in chemical form, the aglycones, daidzein and genistein. They were found only 0,25% on plant, exclusively on leguminous plant. An alternative method have been required to overcome these problem, for example, through plant cell culture technic with heavy metal elicitor. The aims of this research were to study the effect of various concentration of Al3+ elicitor and age of culture for callus growth and accumulation of daidzein and genistein from in vitro Phaseolus vulgaris L. And Phaseolus aureus Roxb callus culture. Stable plant callus culture were established and subculture for three times approximately 4 weeks. The calli were elicitated by Al3+ elicitor with 0, 50, 150, 300 µM concentrations and incubated on 4, 6, and 8 weeks. Qualitative data were determined based in changed calli colour, TLC plate and value of Rf. Quantitative data were determined based in fresh and dry weight of the calli and the content of daidzein and genistein compound. Quantitative data were analized using Anova and continued with DMRT 95%. The results showed that the callus growth more influenced by age of culture. The sixth weeks led to higher fresh and dry weight. The Colour of Calli had been changed from white yellowness become brown at the eighth weeks. The texture of Calli have no difference between control and treatment of elicitor, that was friable until the end of treatment. The Calli with addition of elicitor Al3+ with 150 µM concentrations had significantly highest of fresh weight than control, they were over 0,463 g. The Content of daidzein and genistein on Phaseolus vulgaris L. had significantly increased in addition of Al3+ elicitor with 150 µM concentration. But, the content of daidzein and genistein on Phaseolus aureus Roxb. had significantly increased in addition of Al3+ elicitor with 300 µM concentration. Keywords: Phaseolus vulgaris L., Phaseolus aureus Roxb., elicitor Al3+, Daidzein, Genistein, Callus Growth.
KONSENTRASI HAMBAT MINIMUM EKSTRAK ETHANOL DAUN BELUNTAS (Pluchea indicha) DAN DAUN KEMANGI (Ocimum sanctum L) SERTA CAMPURAN DAUN BELUNTAS DAN DAUN KEMANGI TERHADAP PERTUMBUHAN BAKTERI (Escherichia coli, Staphylococcus aureus) PADA PENDERITA PROSTATITIS Isnainy Hidayati; Sukarjati Sukarjati
STIGMA: Jurnal Matematika dan Ilmu Pengetahuan Alam Unipa Vol 10 No 02 (2017)
Publisher : FMIPA : Universitas PGRI Adi Buana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36456/stigma.vol10.no2.a1030

Abstract

Prostatitis is a disease caused by bacteria, especially (E. coli, S. aureus). Treatment of diseases using antibiotics cause negative effects on the environment, pathogenic bacterial resistance, and antibiotic residues. Therefore, it is necessary to develop an antibacterial alternative from natural ingredients for the treatment of this disease. One of the natural ingredients that are antibacterial is basil leaf extract and beluntas leaves that have antibacterial inhibitory power. This research aims to prove the antibacterial activity of basil leaf extract and beluntas leaves in vitro. The treatments used were P0 (control), P1 (1.56%), P2 (3.125%), P3 (6.25%), P4 (12.5%), P5 (25%), P6 (50% ), P7 (100% ).. The parameters observed were bacterial growth on culture medium. The result of MIC (the lowest concentration of dilution that can inhibit bacterial growth) is seen from the treatment media turbidity and MBC result (the lowest concentration of dilution that can kill bacteria) seen from bacterial colony growth on NAP media. Data were analyzed descriptively. The results showed that basil leaf extract and beluntas leaves can inhibit growth or killing (E. coli, S. aureus) in prostatitis patients. Keywords: Prostatitis, antibacterial activity, microbial inhibition
AKTIVITAS ANTI FUNGI PIGMEN MERAH Penicillium purpurogenum TERHADAP Fusarium oxysporum S. Widayati; Tatang Sopandi
STIGMA: Jurnal Matematika dan Ilmu Pengetahuan Alam Unipa Vol 10 No 02 (2017)
Publisher : FMIPA : Universitas PGRI Adi Buana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36456/stigma.vol10.no2.a1031

Abstract

Penicillium purpurogenum is known to be producing red pigment, pigment produced microbial activity, this research aims to demonstrate the activity of red pigment Penicillium purpurogenum antifungi against diameter and number of Fusarium oxysporum colonies, research done in the laboratory of Microbiology Adi Buana Surabaya University. This study used a randomized complete design (RAL) and Penicillium purpurogenum red pigment treatment concentrations of 0%, 0.5%, 1.0%, 1.5% and Ciprofloxacin 1.0%. Each treatment was repeated 4 (four) times, the research was carried out in vitro on medium PDA. The results showed that the red pigment resistance power P. purpurogenum 1.5% a significant effect (P < 0.05) against growth of fungi F. oxysporum and the number of colonies of fungis F. oxysporum compared red pigment resistance power P. purpurogenum 0% and 0.5%. growth of fungi F. oxysporum on PDA media containing pigment red P. purpurogenum 1.5% significant (P < 0.05) smaller than fungi growth F. oxysporum on PDA media containing pigment red P. purpurogenum 0% and 0.5%. Research results can be concluded that use of red pigment of P. purpurogenum can inhibit growth and the number of colonies of fungis F. oxysporum. Keyword: Red pigment, Penicilliumpur purogenum, Antifungi, Fusarium oxysporum.
Pertumbuhan Dan Kadar Klorofil Tanaman Pakcoy (Brassica rapa L.) Terhadap Cekaman NaCl V. Andriani
STIGMA: Jurnal Matematika dan Ilmu Pengetahuan Alam Unipa Vol 10 No 02 (2017)
Publisher : FMIPA : Universitas PGRI Adi Buana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36456/stigma.vol10.no2.a1032

Abstract

Gravity salt is a soil condition with excessive amounts of soluble salt and adversely affecting plant growth. A number of agricultural crops are able to survive in areas with high salt. This study aims to determine the growth and content of packed chlorophyll packed NaCl at the beginning of the vegetative phase, and determine how optimal concentration in the growth of pakcoy. The research was conducted in experimental garden and basic laboratory of Faculty of Mathematics and Natural Sciences UNIPA Surabaya. The research design was using Random Random Complete with NaCl stress treatment variation. Variation of low NaCl stress treatment consisted of control watered with water (N0), initial NaCl 50 mM + continued 150 mM (N1), initial NaCl 75 mM + continued 150 mM (N2), initial NaCl 100 mM + continued 150 mM (N3), initial NaCl 125 mM + continued 150 mM (N4), and initial NaCl 150 mM + continued 150 mM (N5). Parameters observed were plant growth (plant height, leaf number, leaf width, and root length and biochemistry (leaf chlorophyll) .The quantitative data obtained were analyzed using ANOVA and followed by DMRT test at 95% confidence level.The results showed that the NaCl stress treatment low at the beginning of the vegetative phase raising the height of the plant, the number of leaves, leaf width, root length and the highest leaf chlorophyll content in N1. Keywords: NaCl stress, pakcoy, growth, chlorophyll, vegetative phase
PENGARUH LAMA PENYIMPANAN INFUSED WATER LEMON (Citrus limon) dan MENTIMUN (Cucumissativus L) TERHADAP PERTUMBUHAN BAKTERI M. I. Chandra; Susie Amilah
STIGMA: Jurnal Matematika dan Ilmu Pengetahuan Alam Unipa Vol 10 No 02 (2017)
Publisher : FMIPA : Universitas PGRI Adi Buana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36456/stigma.vol10.no2.a1033

Abstract

This study aims to determine the effect of long storage of infused water lemon (Citrus limon) and cucumber (Cucumissativus L) on bacterial growth. Infused water will be stored for 0 hours, 12 hours, 24 hours, 36 hours, and 48 hours at room temperature. This research uses experimental method. The sample used in this research is infused water lemon and infused water cucumber. The research was conducted in Bacteriology laboratory of Surabaya Health Analyst Department in January 2017 - March 2017. Data analysis was done by observing bacterial colony using total plate number by comparing the two sample treatments. Then proceed with statistical test Independent sample t-test about colony calculation results on infused water lemon and infused water cucumber. The final results show that there is an increase in the number of bacteria in the infused water lemon and infused water cucumber every hour. This increase in the number of bacteria can occur due to improper infusion of water storage, and several factors affecting infused water damage. Keywords : Infused water lemon, infused water cucumber, storage duration, bacterial growth, Total Plate Number.
PENGARUH PEMBERIAN KONSENTRASI BIOINSEKTISIDA DAUN DAN BIJI MIMBA (Azadirachta indica) TERHADAP KEMATIAN ULAT GRAYAK (Spodoptera litura) P. S. Ajiningrum; I. A. K. Pramushinta
STIGMA: Jurnal Matematika dan Ilmu Pengetahuan Alam Unipa Vol 10 No 02 (2017)
Publisher : FMIPA : Universitas PGRI Adi Buana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36456/stigma.vol10.no2.a1034

Abstract

Red spinach (Alternanthera amoena) is one of the vegetable commodities that many people in demand for consumption because of its high nutritional content. Efforts to increase the production of red spinach often experience various obstacles. The main obstacle that limits the productivity of red spinach in the tropics is the number of Plant Disturbing Organisms in the form of pests and potential diseases that can attack and decrease the production of spinach. One of the pests that often attack spinach is the existence of army worm (Spodoptera litura F.). Therefore, farmers need a way to eradicate the pest. This research is experimental using Random Complete Group. The data obtained were analyzed with variant analysis (ANOVA) in one direction and continued with LSD (Least Significant Different) test. Provision of botanical insecticide done by spraying the extract of leaves and seeds mimba with concentration dose 0%, 5%, 10% and 15%. In the final analysis of mortality data of army worm (Spodoptera litura F.) treated with mimba leaf extract obtained significant value of 76.263 at a significant level of 0.05 so it can be concluded that there is influence of mimba leaf extract on mortality of army worm. In the final analysis of mortality data of army worm (Spodoptera litura F.) treated with mimba seed extract obtained significant value of 78.625 at a significant level of 0.05 so it can be concluded that there is an effect of mimba seeds extract on mortality army worm. Keywords: mimba leaf extract, mimba seed extract, red spinach (Alternanthera amoena), army worm (Spodoptera litura F.), botanical insecticide
INHIBITOR PERTUMBUHAN Pyricularia oryzae DENGAN EKSTRAK CAIR DAUN PRONOJIWO (Euchresta horsfeildii) SECARA IN VITRO Astini Astini; Tatang Sopandi
STIGMA: Jurnal Matematika dan Ilmu Pengetahuan Alam Unipa Vol 10 No 02 (2017)
Publisher : FMIPA : Universitas PGRI Adi Buana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36456/stigma.vol10.no2.a1035

Abstract

Has been done research that aims to test the inhibitory power of pronojiwo (Euchresta horsfieldii) leaf extract to the fungus P.oryzae. the experimental study was conducted using a completely randomized design with 9 concentrations of liquid extract of pronojiwo leaf and 1 control. Ie the concentration of each pepeated 4 times and in duplo. Application is done by the method of well by means of liquid extract dripped on the diffusion well, dilusion method by means of liquid extract mixed on the media PDA, method of calculating the amount of fungus conidia by means of liquid extract mixed with media PDB, to determine the inhibition zone of the fungus, the growth of the diameter of the fungus, the number of living conidia. Observations were made after incubation for 7 days. The results showed that liquid extract pronojiwo leaves can inhibit and reduce the growth of fungus P.oryzae, at a concentration of 6,25% with a resistor zone result of 0,9 mm, a growth diameter percentage of 71,2%, and may reduce by 2,9 conidia/ml. Keywords : pronojiwo leaves, growth, reduce , fungus P.oryzae
Uji Aktivitas Sel Kanker dengan menggunakan senyawa Flavonoid dari Lengkuas (Alpinia Galanga) I. A. K. Pramushinta; P. S. Ajiningrum
STIGMA: Jurnal Matematika dan Ilmu Pengetahuan Alam Unipa Vol 10 No 02 (2017)
Publisher : FMIPA : Universitas PGRI Adi Buana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36456/stigma.vol10.no2.a1036

Abstract

Tanaman ini sudah cukup dikenal masyarakat Indonesia dan biasanya digunakan sebagai bumbu campuran pada masakan. Bagian tanaman ini yang biasa digunakan adalah rimpangnya. Rimpang lengkuas ini bisa digunakan sebagai obat tradisional untuk diare, disentri. Pada rimpang lengkuas terdapat senyawa-senyawa flavonoid, glikosida dan diarilheptanoids. Kerangka flavonoid terdiri atas satu cincin aromatik A, satu cincin aromatik B, dan cincin tengah berupa heterosiklik yang mengandung oksigen dan bentuk teroksidasi cincin ini dijadikan dasar pembagian flavonoid ke dalam sub-sub kelompoknya. Penggunaan sel murin leukemia P388 dapat menghambat pertumbuhan sel kanker, karena pada gugus hidroksil (-OH), gugus amida (-CONH) dan gugus dilakton merupakan gugus yang menunjukkan aktivitas menghambat pertumbuhan bakteri dan sel kanker. Ekstraksi senyawa metabolit sekunder dilakukan melalui tahapan ekstraksi dengan methanol kemudian dilanjutkan dengan uji skrining senyawa dan uji antikanker. Analisis ekstrak senyawa rimpang lengkuas dengan perbandingan 1:3 hasil lebih jernih dan baik, analisis uji skrining serbuk rimpang lengkuas didapatkan hasil positif adanya senyawa flavonoid pada uji Bate Smith & Mertcalf dan Uji Wilstater Sianidin, penghambatan sel kanker leukimia P388 50% dari ekstrak metanol lengkuas 16,76 µg/mL Kata kunci: Lengkuas; anti kanker

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