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Ivandini Tribidasari A.
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Makara Journal of Science
Published by Universitas Indonesia
ISSN : 23391995     EISSN : 23560851     DOI : https://doi.org/10.7454/mss
Core Subject :
Makara Journal of Science publishes original research or theoretical papers, notes, and minireviews on new knowledge and research or research applications on current issues in basic sciences, namely: Material Sciences (including: physics, biology, and chemistry); Biochemistry, Genetics, and Molecular Biology (including: microbiology, physiology, ecology, taxonomy and evolution); and Biotechnology.
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Articles 880 Documents
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Hepatitis B Surface Antigen and Viral DNA Detection and Prevalence in Nige-ria Desmond, Abazuh Uchenna; Tola, Ohiengbomwan Onaiwu; Isaac, Adeolu; Mercy, Yusuf
Makara Journal of Science Vol. 24, No. 3
Publisher : UI Scholars Hub

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Abstract

Hepatitis B infection is a major public health issue with a high mortality rate. It is caused by hepatitis B virus (HBV), a small incomplete hepadnavirus with several open reading frames (ORF). High mutation rates of the HBV ORFs due to the virus’ replicating strategy are associated with the development and emergence of mutant strains, which may result in occult infections that are undetectable by conventional diagnostic assays. In this study, we first investigated the presence and prevalence of hepatitis B surface antigen (HBsAg) and HBV DNA among 204 patients visiting the specialist hospital in Osogbo, Nigeria, by using the Acumen diagnostic test strip kit and PCR amplification of the surface antigen gene. Then, we compared the positive results detected by both assays. The mean age of the participants was 30.5 years. Twenty-two (10.78%) cases were positive in the HBsAg test, and 69 (33.82%) cases were positive for HBV DNA. Fifty-four false-negatives (26.47%) and four false-positives (1.96%) were detected by HBsAg. The rapid diagnostic test kit is less sensitivity and has lower detection capability compared with the PCR-based assay, which indicates the presence of mutant HBV strain(s). The results of this work demonstrate that HBsAg is endemic in Nigeria and that HBV DNA is highly prevalent among the population. Our findings highlight the need for immediate action to prevent the further spread of infection.
Malonic Acid as A Green and Efficient Catalyst for the Mass-scale Synthesis of Pyrrole Medicinal Drugs Mohamadpour, Farzaneh
Makara Journal of Science Vol. 24, No. 3
Publisher : UI Scholars Hub

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Abstract

A green and naturally biodegradable malonic acid synthesis of highly substituted dihydro-2-oxopyrrole derivatives has been accomplished via one-pot four-condensation of amines (aromatic or aliphatic), dialkyl acetylenedicarboxylate, and formaldehyde under mild reaction conditions. The notable advantages of the present procedure are a green, low cost, and efficient catalyst; operational simplicity; no need for chromatographic purification steps; short reaction times; and good to high yields.
Ambient Noise-Based Mapping of Bedrock Morphology and Potential Fissure Zone in East Tanjung Karang, Bandar Lampung, Lampung, Indonesia Ipmawan, Vico Luthfi; Permanasari, Ikah Ning Prasetiowati; Suhendi, Cahli
Makara Journal of Science Vol. 25, No. 2
Publisher : UI Scholars Hub

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Abstract

As a business center and the most populous subdistrict, East Tanjung Karang in Bandar Lampung, Lampung, Indonesia, is considered an area with excessive groundwater exploitation. This activity can trigger ground fissures that can consequently cause damage to buildings and roads. In this study, microtremor recordings from 17 sites were collected and analyzed by using the horizontal to vertical spectral ratio and ellipticity curve method. Results showed that the ground profiles of shear wave velocity from 17 sites ranged from 143.5 m/s to 1752.46 m/s, and they could be used to determine sediment layer and its thickness based on the SNI 1726-2012 criteria. The thickness of the bedrock varied from 8.18 m to 117.18 m. Bedrock morphology was obtained by subtracting the sediment thickness from the altitude value. The bedrock morphology and slope were then used to construct a potential fissure map of the area between Y16 and Y17 and between Y26 and Y27, which had high bedrock slopes (more than 45°). The ground fissure potential in these areas was higher than that in other areas. Such areas also had a geological hazard potential from ground fissures caused by excessive groundwater exploitation. Our study could be used by authorities as a basis for preventing subsidence-related disasters in this subdistrict.
Cloning of pe11 (LipX, Rv1169c) gene of Mycobacterium tuberculosis Beijing strain to pcDNA3.1 plasmid vector Supardi, Lulut Azmi; Rukmana, Andriansjah; Sjatha, Fithriyah
Makara Journal of Science Vol. 25, No. 1
Publisher : UI Scholars Hub

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Abstract

Tuberculosis (TB) is an infectious disease caused by Mycobacterium tuberculosis. It is a persistent global health problem with a high mortality rate. Currently, TB is controlled by administering the Bacillus Calmette-Guerin (BCG) vaccine, but the effectiveness of its protection varies among individuals in a population. The pe/ppe gene family comprises a typical group of genes that play a role in avoiding the host immune response and inducing persistent TB infection. Based on in silico analysis, the pe11 gene has estimated immunogenicity and potential as a TB seed vaccine candidate. The pe11 gene from an Indonesian isolate of an M. tuberculosis Beijing strain was amplified by polymerase chain reaction (PCR) and inserted into the mammalian expression vector pcDNA3.1. The recombinant vector pcDNA3.1-pe11 was used to transform Top10 competent Escherichia coli. Clones from the transformation were subjected to colony PCR to confirm the direction of the insert. Sequencing was performed to confirm the correctness of the insert sequence. In this study, the pe11 gene was successfully cloned into the pcDNA3.1 vector in the correct direction to assure PE11 expression. No mutations were found in the pe11 gene insert, compared with the M. tuberculosis H37Rv sequence as the standard. A pcDNA3.1 vector containing the pe11 gene derived from an M. tuberculosis Beijing strain was successfully constructed.
Physical and Chemical Properties of Soil, Water and Air around Ukawu Pb-Zn Mine, Southeastern Nigeria Nwachukwu, Nwachukwu Arthur; Udie, Sikakwe Gregory; Nchedo, Eluwa Ndidiamaka; Ukamaka, Uwa Clementina; Olaosebikan, Oluwatoyin Oluwatosin
Makara Journal of Science Vol. 25, No. 2
Publisher : UI Scholars Hub

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Abstract

In this study, analysis of water, and soil samples, air quality, and noise levels in Ukawu Pb-Zn mine were examined. The pH of the water samples, at 6.7, exceeded permissible levels for potable water established by the National Environmental Standard Regulatory Agency (NESREA), European Union, and World Health Organization (i.e., 7.0-8.5). The soil pH was 5.2, which indicates acidity due to acid mine drainage. Low soil pH is evidence of acid mine drainage. All cations detected in water and soil were below standard limits considered harmful by the NESREA. Measurements of precursor gases, such as O3, NO2, CO and SO2, to determine air quality showed levels below the air quality standards prescribed by the NESREA. Thus, the air quality around the mine is not polluted by these gases. Noise levels around the mine were below the 85dB limit considered deleterious to humans. Comparison of the noise levels detected around the mine with Indian showed that the values obtained in Ukawu mine exceed the permissible daytime values for industrial, commercial, residential, and silence zones. This research could serve as a benchmark for environmental contamination studies at the regional and global scales.
Yeasts Isolated from Traditional Brem Bali Show Stress Tolerance Phenotype against Fermentation-Related Stresses Lenka, Audria Bayu; Astuti, Rika Indri; Listiyowati, Sri
Makara Journal of Science Vol. 25, No. 1
Publisher : UI Scholars Hub

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Abstract

The search for microbes, primarily yeasts with unique characters such as the tolerance against fermentation-related-stresses, is gaining significant interest nowadays. Traditionally made alcoholic beverages can be used as sources for such yeasts, given that during fermentation and storage, microbes may develop stress tolerance responses leading to naturally stress-tolerant yeast strains. In this study, we used an alcoholic beverage, that is, Brem Bali, as the source of potential yeast isolates. We isolated nine yeast isolates from two traditional Brem Bali products. All isolates showed tolerance against high glucose stress (40–50%) and sensitivity against high-temperature stress (37–50 °C). Notably, isolate BT5 showed tolerance phenotype against ethanol stress (up to 12.5%). Notably, the ethanol stress tolerance phenotype shown by isolate BT5 was unlikely correlated to the ability of the isolates in combating other stresses. Based on the internal transcribed spacer sequence, isolates BT2, BT5, and BT6 shared high similarity with Wickerhamomyces anomalus (98%). Further approaches may be needed to clarify the identity of these isolates. Based on our study, isolate BT5 bears potential as a fermentation agent based on its ability to combat high glucose and ethanol stresses.
Efficient and Mild Four-component Process for the Synthesis of Highly Substituted Dihydro-2-oxopyrroles using ZrOCl2∙8H2O as an Environmental-ly Friendly Catalyst Mohamadpour, Farzaneh
Makara Journal of Science Vol. 24, No. 4
Publisher : UI Scholars Hub

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Abstract

This study investigated the catalytic ability of ZrOCl2∙8H2O as a mild, environmentally benign nature, and economical catalyst for the multi-component efficient synthesis of biologically active highly substituted dihydro-2-oxopyrrole derivatives with excellent yields and short reaction times. This procedure has several advantages, including use of mild, nontoxic, and inexpensive catalysts, one-pot synthesis, environmentally benign nature, simple operational procedure, and highly efficient conditions.
Computational Analysis of Chromophore Tripeptides FollowingFusion of Enhanced Green Fluorescent Protein and Cell-penetrating Peptides Widyaningtyas, Silvia Tri; Pratiwi, Ekawati Betty; Bela, Budiman
Makara Journal of Science Vol. 24, No. 4
Publisher : UI Scholars Hub

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Abstract

Cell-penetrating peptides (CPPs) are small peptides that can transfer other materials into a cellular compartment. In this research, we studied the effect of fusion of new CPPs to the N-terminal of enhanced Green Fluorescent Protein eGFP on the ability of the latter to fluoresce. Results showed that the recombinant protein CPPs-eGFP could be successfully expressed in Escherichia coli. In contrast to E. coli expressing wild-type eGFP, which could fluoresce under ultraviolet (UV) or visible light, E. coli expressing CPPs-eGFP lost their ability to fluoresce. PyMol, a molecular visualization system, revealed that fusion of the new CPPs to the N-terminal of eGFP alters interactions between chromophore-forming tripeptides and the adjacent amino acids of other tripeptides. Disrupting peptide interactions induced structural changes in eGFP that caused it to lose its fluorescence ability. We suggest performing computational analyses to predict the biological function of new fusion proteins prior to starting laboratory work.
Association between Dietary Macronutrient Intake and High-Sensitivity C-Reactive Protein Levels among Obese Women in Kuantan, Malaysia Shahadan, Siti Zuhaidah; Daud, Azlina; Ibrahim, Muhammad; Md. Isa, Muhammad Lokman; Draman, Samsul
Makara Journal of Science Vol. 24, No. 4
Publisher : UI Scholars Hub

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Abstract

Elevated high-sensitivity C-reactive protein (hs-CRP) levels may be associated with an increased risk of cardiovascular disease (CVD). In general, an individual’s dietary intake may influence the hs-CRP level. However, evidence on the influence of dietary macronutrient intake on hs-CRP levels among obese Malaysian women remains fragmented. Therefore, this study aims to investigate the association between the hs-CRP level and dietary macronutrient intake of obese adults living in Kuantan. The assessment of 24-hour dietary recall and venous hs-CRP levels were investigated in 67 women with a body mass index of 27.5 to 39.9 kg/m². The findings revealed that obese women living in Kuantan had elevated hs-CRP levels (median = 7.95 mg/L, IQR = 7.90) and a significant negative correlation between the hs-CRP level and total dietary fiber intake (r = 0.205, p =.014). In conclusion, this study suggests that certain macronutrients, particularly dietary fiber, seem to be associated with elevated hs-CRP in obese women. Hence, this information could help assess and manage low-grade chronic inflammation and underlying obesity-related conditions.
Modeling of Aqueous Root Extract Compounds of Ruellia tuberosa L. for Alpha-Glucosidase Inhibition Through in Silico Study Safitri, Anna; Sari, Dewi Ratih Tirto; Fatchiyah, Fatchiyah; Roosdiana, Anna
Makara Journal of Science Vol. 25, No. 1
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Abstract

This study aims to analyze the inhibitory activities of aqueous root extract compounds of Ruellia tuberosa L. toward alpha-glucosidase protein by computational docking analysis. Three major compounds contained in the extracts (i.e., betaine, daidzein, and hispidulin) were selected as ligands; quercetin and acarbose were used as the reference. Computational docking analysis was performed using the HEX 8.0.0 program and visualized using the Discovery Studio Visualizer v19.1.0.18287 (2019 version) on the basis of the scoring functions. The interactions between ligands and alpha-glucosidase protein showed different binding patterns. The types of bonds involved in the interaction between the enzyme and these ligands were hydrogen and hydrophobic bonds. Energy generated from docking of betaine, daidzein, hispidulin, quercetin, and acarbose to alpha-glucosidase protein were −167.6, −249.5, −251.2, −241.5, and −322.1 cal/mol, respectively. Acarbose had the lowest energy, indicating that it has the strongest interaction with alpha-glucosidase, followed by hispidulin, daidzein, quercetin, and betaine. Amino acid residues that interacted with the ligands were His717, Met363, Arg608, Pro361, Phe362, Leu865, Glu869, Arg594, andAsp356. The current research shows that R. tuberosa L. aqueous root extracts have the potential to be used as an inhibitor for the alpha-glucosidase protein and as an antidiabetic agent. Nonetheless, further studies are needed to support this modeling study.

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