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Journal of Biotechnology and Natural Science

JBNS
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Published by Universitas Ahmad Dahlan

ISSN : - EISSN : 28082508 DOI : -

Core Subject : Science, Agriculture,

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology

Aims • The journal provides published articles devoted to all aspects of science in the field of biology and biotechnology advancement. Scopes • Biotechnology • Genetics • Microbiology • Botany • Zoology • Ecology • Environmental Biology • Molecular Biology

Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Pertanian dan Biologi (Lain-Lain)

Articles 52 Documents

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Ethanolic Extracts of Shallot Leaves (Allium ascalonicum L.) as Botanical Pesticide for Controlling Fall Armyworms (Spodoptera fugiperda J.E. Smith) Putri, Diah Asta; Nur Aini, Pradipta
Journal of Biotechnology and Natural Science Vol. 1 No. 2 (2021): December
Publisher : Universitas Ahmad Dahlan

The application of botanical pesticide is a positive long term approach to control S. frugiperda. Shallot leaves contain acetogenin compound, hence it has the potential to become botanical pesticide. This study is aimed at finding out the effects of ethanolic extract of shallot leaves on S. frugiperda larvae in vitro. Extract is prepared using maceration method with ethanol solvent. The method of treatment is Leaf Dipping. The study applies Completely Randomized Design with concentration of shallot leaf extract as treatment at 0 ppm, 250 ppm, 500 ppm, 750 ppm dan 1000 ppm. Each treatment consists of four repetitions. Examination is conducted for 24 hours. Examination parameter is mortality of larvae and LC50. Data are analyzed with One Way ANOVA (proceeded with LSD) and Probit analysis. The results suggest that shallot leaf extract at the concentration of 1000 ppm appears to be the most effective on the mortality of larvae. The obtained LC50 value is 263,02 ppm. The study indicates that shallot leaf extract can be applied as an alternative synthetic insecticide to control ini S. frugiperda.

The Meiofaunal Diversity in Progo and Opak River Estuaries Suwartiningsih, Nurul; Muhammad Ardhi, Fauzan
Journal of Biotechnology and Natural Science Vol. 1 No. 2 (2021): December
Publisher : Universitas Ahmad Dahlan

The Estuaries of Progo and Opak rivers, being the terminus of all rivers flowing through the Special Region of Yogyakarta, are highly susceptible to pollution which results in decreased water quality. The use of meiofauna as a bioindicator to assess water quality in the estuaries of Progo and Opak rivers has never been made. This study aims to determine the diversity of meiofauna at the estuaries of Progo and Opak rivers. Stations and sampling points are determined using purposive sampling method. Extraction is conducted using water ice treatment method. The meiofauna obtained from the sediment screening are then identified, calculated and analyzed to find out the index values of abundance, dominance, diversity, and evenness. The results showed that the highest meiofauna abundance index was 247,333 ind. m-2 at the estuary of Progo river station 1 at the time of the first pick-up. While the lowest meiofauna abundance was 13,333 ind. m-2 at station 2 of Opak river estuary during the first take. The dominance at the estuaries of Progo and Opak rivers is in the moderate category. The meiofauna diversity in both river estuaries is categorized as low to moderate. Evenness in both estuaries is uneven at several stations. The meiofauna diversity at the estuaries of Progo and Opak rivers is low to moderate, although the water quality still meets the quality standards.

Environmental Quality of Prambanan Temple and Ratu Boko Temple’s Green Open Space based on Soundscape and Guild of Bird Utami, Inggita; Yahya, Eva Nurulia; Afriandini, Wida; Dwipayana, Ajeng Silvi; Utami, Salsa Aprilia; Meilina Arliani, Baiq Dara; Ramadhani, Khairunnisrina
Journal of Biotechnology and Natural Science Vol. 2 No. 1 (2022): June
Publisher : Universitas Ahmad Dahlan

Sleman Regency has the most temple cultural heritage in Yogyakarta, supported by Green Open Space (GOS). The area is a habitat for urban birds and can be used as an indicator of environmental quality. This study aims to analyze the environmental quality based on the soundscape and character of the bird community in the GOS of Prambanan Temple and Ratu Boko Temple. Sound recordings were taken with a smartphone, and the recording time was set using the Arbimon touch in the morning, afternoon, and evening. The sound recordings are used to calculate acoustic indexes. The sound recordings and spectrograms are processed to identify bird species and calculate the Bird Community Index. The number of bird species was higher in the Ratu Boko area. According to the NDSI value, the sound of biophony at Ratu Boko Temple is more dominant than anthrophony and geophony. The environmental quality in the Prambanan Temple area is in the low category than in the Ratu Boko Temple area, which is classified as a medium category. The area's topography, the arrangement of vegetation, and the noise of vehicles due to the proximity to the highway affect the environment quality in temple areas.

Vinca alkaloids effects on the morphological characters of Canavalia ensiformis Putri, Diah Asta; Rahyuni, Mita; Sarwati, Mega
Journal of Biotechnology and Natural Science Vol. 2 No. 1 (2022): June
Publisher : Universitas Ahmad Dahlan

Productivity and quality of crops can be increased through polyploidy plants induced by antimitotic compounds such as vinka alkaloids.The use of vinka alkaloids has never been applied to Sword bean (Canavalia ensiformis), a tropical legume belonging to the Fabaceae family. This study aimed to determine the effects of vinka alkaloids treatment on the morphological characters of Sword bean. The treatment consist of four different vinka alkaloids concentrations (0.5%, 1.00%, 1.50%, 2.0%, and untreated (0.0%) act as control. The plants were arranged in a completely randomized design. Treated plants were characterized based on morphological traits such as plant height, leaf area and dry seed weight. The results obtained revealed significant difference (p≤0.05) in the morphological traits of the treated plants when compared with the control. Overall, 2.0% vinka alkaloids concentration resulted in higher average plant height by 193.8 ± 1.86, leaf area increments by 88.6 ± 5.1 (left leaf), 115.1 ± 4.7 (middle leaf), 86.2 ± 3.8 (right leaf) and the dry seed weight by 1.7 ± 3.6. The improvement of the traits is concentration dependent and increases with increment in vinka alkaloids concentration. Thus, we suggested that 2.0% concentration should be employed in improving Sword bean growth and yield.

Morphological Variation of “Jamur So” (Scleroderma sp.) from Purworejo Regency, Central Java Pratiwi, Ambar; Putri, Diah Asta; Gultom, Putri Syifa; Indah, Rosiana; Sasongko, Hadi
Journal of Biotechnology and Natural Science Vol. 2 No. 1 (2022): June
Publisher : Universitas Ahmad Dahlan

Fungal diversity is high in Indonesia, one of which is Scleroderma sp. Scleroderma forms a symbiotic relationship with Gnetum gnemon. Its fruiting body is puff-ball shaped, and its gleba can be consumed because of its taste and nutritious. Study on the Scleroderma’s diversity in Purworejo Regency has not been done. In this study, morphological characteristics were used as main identification character. Scleroderma samples were collected from Grabag, Kemiri, and Kaligesing and then observed their morphology and spore characteristics. Morphological traits observed on fruit body shape (globose and kidney-shaped); and ornamentation on peridium layer (cracked, nodule and peeling). Chemical content, temperature, and humidity of the soil were also observed as supporting data.

Growth Response and Biochemistry of Red Spinach (Amaranthus tricolor L.) with the Application of Liquid Organic Fertilizer Lemna sp. Pratiwi, Ambar; Roka Aji, Oktira; Sumbudi, Milliniawati
Journal of Biotechnology and Natural Science Vol. 2 No. 2 (2022): December
Publisher : Universitas Ahmad Dahlan

Red spinach is widely cultivated and consumed by the public because it has high antioxidants, vitamins (A, C, E), protein, carbohydrates, fats, minerals, iron, magnesium, manganese, potassium and calcium. P giving POC can increasered spinach biomass. Nutrients in POC have been decomposed so that they are easily absorbed by plants. This study aims to analyze the giving of POC Lemna sp. on growth and biochemical content of red spinach and determine the concentration of POC Lemna sp. effective for red spinach (Amaranthus tricolor L.). The study used a completely randomized design with 3 replications and 3 treatments. The independent variable in this study was the concentration of liquid organic fertilizer Lemna sp. (A=0%, B=5%, and C=10%). Data were analyzed by ANOVA test and DMRT test at 5% level. The results showed that the administration of POC Lemna sp. gave significantly different results on the A (0%) treatment of plant height, stem diameter, and flavonoid parameters. Giving POC Lemna sp. can increase the growth and flavonoid content of red spinach. The concentration of liquid organic fertilizer Lemna sp. 5% effective for the growth of red spinach (Amaranthus tricolor L.).

Inventory of Terrestrial Orchids in the Baturraden Botanical Garden of Central Java Astuti, Sutan Nur Chamida Tri; Aisah, Siti; Kurniawan, Ardyan Pramudya
Journal of Biotechnology and Natural Science Vol. 2 No. 1 (2022): June
Publisher : Universitas Ahmad Dahlan

Baturraden Botanical Garden located on the slopes of Mount Slamet in the south has a high enough humidity so it is ideal for plant growth, especially orchids. As an ex-situ plant conservation area, the Baturraden Botanical Garden plays an important role in preserving the diversity of orchids in it. The purpose of this study was to find out the types and descriptions of terrestrial orchids in the Baturraden Botanical Garden area of Central Java. The method used in this study is a 400 m line transect method with an inter-point distance of 100 m each and data collection is carried out at 10 m each on the right and left of the line transect. Hasil research shows that there are 13 species derived from 10 genus of soil orchids. The types that have been found are Goodyera rubicunda (Blume) Lindl., Calanthe pulchra (Blume) Lindl., Spathoglottis plicata Blume, Cryptostylis javanica J.J.Sm., Appendicula alba Blume, Phaius callosus (Blume) Lindl., Lepidogyne longifolia Blume, Tainia elongata J.J.Sm., Goodyera colorata Blume, Malaxis sp. Soland. ex Sw., Goodyera sp. R. Br., Goodyera procera (Ker-Gawl.) Hook., and Plocoglottis sp. Blume.

Species Diversity and Dominance of the Aranaemorphae Sub-Order in the 90-Day-Old Rice Generative Phase Putra, Ichsan Luqmana Indra; Pertiwi, Pelangi Mayang Sukma
Journal of Biotechnology and Natural Science Vol. 2 No. 2 (2022): December
Publisher : Universitas Ahmad Dahlan

Spiders have an important role in ecosystems, in particular agricultural ecosystems. Spiders play the role of natural enemies capable of helping farmers by eating insect pests. The purpose of this study was to identify the type of Sub-Order Aranaemorphae and calculate the level of diversity and dominance of the type of Sub-Order Aranaemorphae in the generative phase of rice aged 90 days. The study was conducted in April-September 2022 in the rice field area of Tamanan Village. The determination of the research location was carried out using the purposive sampling method with the criteria of a rice field area of 2500 m2 and the age of rice plants ranging from 90 days. Sampling was carried out on 9 plots with a plot size of 21 x 4 m2 each. Sampling was carried out by hand picking and indirect methods with sweep nets, pitfall traps, and yellow pan traps. The species of Sub-Order Aranaemorphae found in this study were 12 species, namely Oxyopes sp., O. lineatipes, O. javanus, Atypena formosana, Tetragnatha vermiformis, Pardosa pseudoannulata, Lycosa sp., L. pseudoannulata, Lycosa sp. 1, Araneus inustus, Clubiona sp. and Thomisus Sp. The conclusion of this study were the diversity index value (H') of the Aranaemorphae Sub-Order obtained in this study was 2.133 with a moderate category, and the dominance index value (D) of the Aranaemorphae Sub-Order obtained was 0.1785 with a low category. The total number of spider individuals obtained was 173 individuals.

Toxicity of Copper (Cu) and Chromium (Cr) on the Seed Germination of Mung Bean (Vigna radiata L.) Astuti, Sutan Nur Chamida Tri; Nugroho, Andhika Puspito
Journal of Biotechnology and Natural Science Vol. 2 No. 2 (2022): December
Publisher : Universitas Ahmad Dahlan

Rapid industrial growth may have implications for an increase in cases of pollution of soil ecosystems through untreated waste disposal. Copper and chromium are metals found in some types of industrial wastewater, such as electroplating and silversmithing, that are essential at low concentrations but toxic at high concentrations. Vigna radiata was chosen as the test organism because it has been reported that the yield and production of the crop have decreased worldwide due to heavy metal pollution. This study aims to evaluate individual and mixed Cu and Cr toxicity in the seed germination of V. radiata. The individual and mixed Cu and Cr toxicity test was started with a range-finding and definitive test for 96 h. The toxicity level of the individual metal was expressed as IC50-96 h by probit analysis. For the mixed test, the organism was exposed to concentration ratios of Cu and Cr: 10%:90%; 35%:65%; 50%:50%; 65%:35%; and 90%:10% of individual IC50-96 h, each conducted for 96 h. The results showed that the individual IC50-96 h of Cu was 127.4 mg/L, while for Cr, it was 615.23 mg/L, indicating that Cu is more toxic than Cr. The mixed test showed that the IC50-96 h of Cu was 247.5 mg/L, 579.85 mg/L for Cr. The highest inhibition value at the mixed test was at a Cu and Cr concentration ratio of 90%:10%. Based on the mixed test, the interaction of Cu and Cr resulted in an additive effect (CI=1).

Inventory of Collembola Species Around Ahmad Dahlan University Campus 4 And Its Surroundings Hanifuddin, Afnan Saud; Kamalia, Lulu Atikah
Journal of Biotechnology and Natural Science Vol. 2 No. 2 (2022): December
Publisher : Universitas Ahmad Dahlan

Collembola has an important value for the environment as a bioindicator in maintaining the balance of the ecosystem. Land conversion in the UAD campus 4 area can reduce the diversity of Collembola. This study aims to find out the level of diversity of Collembola species that exist on campus 4 of UAD and its surroundings, and also find out which areas have the highest and lowest number of individuals. The research area for sampling in this study is the UAD 4 campus area, vacant land, housing and rice fields. Sampling was carried out as many as four repetitions. The collembola trapped in the pitfall trap is then moved in a microtube. The collembola that has been obtained is then identified up to the species level. The data obtained were analyzed by descriptive analysis. The types of collembola obtained at campus 4 UAD and its surroundings are Folsomia sp,, Isotoma sp., Isotomurus sp., Entomobrya sp., Sminthurus sp., Hypogatrusa sp, and Lepidocyrtus sp. The collembola species that is most commonly found are from Entomobrya famili with 106 individuals and the least individual from Sminthuridae famiili with 4 individuals.

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Contact Name
Ichsan Luqmana Indra Putra

Contact Email
ichsan.luqmana@bio.uad.ac.id

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+6283869807245

Journal Mail Official
journal.bns@bio.uad.ac.id

Editorial Address
Jl. Ahmad Yani, Ring Road Selatan, Kragilan, Tamanan

Location
Kota yogyakarta,

Daerah istimewa yogyakarta

INDONESIA

Abstract

Abstract

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Abstract

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Abstract

Abstract

Abstract

Home Page

OAI Link

Editorial Team

Contact

Reviewer

Google Scholar

Contact Name Ichsan Luqmana Indra Putra

Contact Email ichsan.luqmana@bio.uad.ac.id

Phone +6283869807245

Journal Mail Official journal.bns@bio.uad.ac.id

Editorial Address Jl. Ahmad Yani, Ring Road Selatan, Kragilan, Tamanan

Location Kota yogyakarta, Daerah istimewa yogyakarta INDONESIA

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Contact Name
Ichsan Luqmana Indra Putra

Contact Email
ichsan.luqmana@bio.uad.ac.id

Phone
+6283869807245

Journal Mail Official
journal.bns@bio.uad.ac.id

Editorial Address
Jl. Ahmad Yani, Ring Road Selatan, Kragilan, Tamanan

Location
Kota yogyakarta,

Daerah istimewa yogyakarta

INDONESIA