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Ichsan Luqmana Indra Putra
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INDONESIA
Journal of Biotechnology and Natural Science
Published by Universitas Ahmad Dahlan
ISSN : -     EISSN : 28082508     DOI : -
Core Subject : Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology
Aims • The journal provides published articles devoted to all aspects of science in the field of biology and biotechnology advancement. Scopes • Biotechnology • Genetics • Microbiology • Botany • Zoology • Ecology • Environmental Biology • Molecular Biology
Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Pertanian dan Biologi (Lain-Lain)
Articles 47 Documents
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Morphometric Variations of Chalcorana chalconota (Schlegel, 1837) in Four Populations in Jatimulyo Kulon Progo Hanifuddin, Afnan Saud; Aini, Khuratul; Suwartiningsih, Nurul
Journal of Biotechnology and Natural Science Vol. 2 No. 2 (2022): December
Publisher : Universitas Ahmad Dahlan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (872.459 KB) | DOI: 10.12928/jbns.v2i2.6965

Abstract

Jatimulyo is an ecotourism area consisting of several areas, each of which has a different height and abiotic factors. This can affect the morphometric character of frogs including Chalcorana chalconota. Research on specific morphometric variations of Chalcorana chalconota in Jatimulyo ecotourism has never been conducted. The purpose of this study was to analyze the distinguishing character of four frog populations as well as analyze the morphometric variation of the Chalcorana chalconota frog in Jatimulyo ecotourism. The research samples were taken using the visual encounter survey method and direct collection in the field in all four populations. Morphometric measurements use 32 characters. The measurement data obtained were analyzed using the Kruskall-Wallis test followed by UPGMA to see dissimilarity using the MVSP 3.1 program. The results showed that of the 32 characters observed and analyzed, there were nine characters that had a p<0.05 value which means it is significant to distinguish the four populations. The nine characters are (HW), (TD), (MFE), (MBE), (HAL), (LAL), (FOL), (IN), (MTL). LK, DT, JMMD, JMMB, JIN, PMD, PBR, PTJ4, PTM. The frog Chalcorana chalconota population of Kembang Soka has a high morphometric similarity with the population of Kedung Pedut. The Mudal River Population and the Bull Kedung Population have a considerable morphometric similarity with the Soka Flower and Pedut Flower Populations. This study can be concluded that the morphometric variation of Chalcorana chalconota in Jatimulyo ecotourism is influenced by altitude factors.
Effect of An Additional Amount of Fish and Fermentation Time on The Sensory Properties of The Lemea Aziz, Abdul; Aji, Oktira Roka
Journal of Biotechnology and Natural Science Vol. 3 No. 1 (2023): June
Publisher : Universitas Ahmad Dahlan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.12928/jbns.v3i1.8261

Abstract

Lemea, a traditional fermented food of the Rejang people in Bengkulu, is made from young bamboo and fish. The fish commonly used in the making Lemea is Mozambique tilapia or Mujair (Oreochromis mossambicus). The fermentation process typically lasts between 3 to 7 days. This study aimed to assess the sensory characteristics, including taste, color, texture, and aroma, of Lemea, based on the preferences of panelists. The variations examined were fermentation duration, and the amount of Mujair fish added. The research followed a completely randomized design, incorporating three fermentation durations (24, 96, and 144 hours) and three fish quantities (250 grams, 500 grams, and 750 grams). Sensory evaluations were conducted on the fermented samples, encompassing taste, aroma, texture, and color. The data revealed that the most favored taste and texture of Lemea were achieved with 500 grams of fish and a fermentation duration of 144 hours (J2W3). Likewise, the preferred aroma and color were observed in the sample with 750 grams of fish and 24 hours of fermentation (J3W1). The data analysis indicated that the sample with 500 grams of fish and a fermentation duration of 144 hours (J2W3) exhibited the most favorable sensory characteristics (taste, aroma, texture, and color). Thus, the quantity of fish added and the duration of fermentation influenced the sensory attributes of Lemea.
Effect of Banana Bark and Cow's Blood Meal Compost on the Green Lettuce Plants (Lactuca sativa L.) Growth Pratiwi, Ambar; Wulandari, Ayu
Journal of Biotechnology and Natural Science Vol. 3 No. 1 (2023): June
Publisher : Universitas Ahmad Dahlan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.12928/jbns.v3i1.8365

Abstract

Green lettuce (Lactuca sativa L.) is a vegetable crop that is in great demand by the public, but its production is relatively low so that it cannot meet the high market demand. One of the efforts to increase the production of green lettuce plants is by applying organic fertilizer. The purpose of this study was to determine the content of C, N, P, K elements, the effect of compost application for growth and optimal dosage. The research method used a complete randomized design (CRD) four treatments namely Control, A (dose of 60 g), B (dose of 120 g) and C (dose of 180 g) with 5 replications. Data from the study were analyzed using the Anova test, significant data continued with the DMRT 5% further test. Testing the effect with simple liner regression analysis. The results showed that compost gave a blackish brown color after 35 days and had no odor and obtained nutrient values of C (41,77%), N (6,92%), P (0,09%) and K (0,12%). The treatment of compost variations produced plant height of 26,80 cm, number of leaves of 10,60 leaf, plant wet weight of 22,40 gram, root weight of 0,57 gram, root length of 12,94 cm leaf area of 130,06 cm² with significant results, while chlorophyll A (0,19 mg/L) and total (0,616 mg/L) levels had insignificant results. The conclusion of the research is that the compost of banana stalks and cow blood meal contains C by 41,77%, Total N by 6,92%, Total P by 0,09% and Total K by 0,12%. Banana pith compost and cow blood flour had a significant effect on all parameters of green lettuce plant growth except leaf chlorophyll content at the most optimal dose was treatment A (dose of 60 g).
Spodoptera frugiperda J.E SMITH Attack on Non-Maize Crops in Bantul Regency Putri, Tasya Triana; Putra, Ichsan Luqmana Indra
Journal of Biotechnology and Natural Science Vol. 3 No. 1 (2023): June
Publisher : Universitas Ahmad Dahlan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.12928/jbns.v3i1.9287

Abstract

Spodoptera frugiperda is a polyphagous insect that has a main host of corn plants and attacks corn plants in the vegetative to generative phase. Besides corn, S. frugiperda has been reported to have other alternative host plants. The purpose of this study was to analyze the type of S. frugiperda attack most often found on alternative host plants and analyze the types of plants other than corn that are most often used as alternative hosts by S. frugiperda in Bantul Regency. This research was conducted in Bantul Regency in January-April 2023. Samples were taken from two villages from each sub-district in Bantul Regency. The research was conducted using survey method to determine the village as the research location and purposive method for sampling the alternative host plants. Samples taken were plants around corn fields with symptoms of S. frugiperda attack in the form of perforated leaves or traces of feces from feeding activities or S. frugiperda egg packages. The results of this study showed that 29 plant species from 13 families were attacked by S. frugiperda in Bantul Regency. The most common type of S. frugiperda attack was bite marks (60%). The most commonly attacked plant species came from the Poaceae family, namely elephant grass (Pennisetum purpureum). The conclusion of this study is that S. frugiperda has attacked plants other than corn in 17 sub-districts in Bantul Regency.
Morphological Structure of the Body Setae as Mechanoreceptor on the Mesonotum of Telenomus remus Nixon (Hymenoptera, Platygastridae) Wikantyoso, Bramantyo; Putra, Ichsan Luqmana Indra
Journal of Biotechnology and Natural Science Vol. 3 No. 1 (2023): June
Publisher : Universitas Ahmad Dahlan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.12928/jbns.v3i1.9424

Abstract

Telenomus remus is parasitoid on many Lepidpoteran. Morphological analysis on T. remus is important to understand their behavior and assest the quality of parasitoid comes from the mass rearing process. However, the information of T. remus body setae cuticle characteristics were limited. This study aimed to explore the body setae on the mesonotum of of T. remus by using electron microscopy analysis and characterize their cuticle characterisics to infer the putative function of the body setae. The results showed structures that support the body setae on the mesonotum as mechanoreceptor. The structure included the long and dangling seta peg, a socket, and a gap between a socket and the base of the seta peg. The putative function related to the biology and behavior of T. remus were discussed.
Microplastic Contamination of Rainwater on the Highway with Different Elevations in Yogyakarta Province Indonesia Gunawan, Almaida Khansa; Utami, Inggita; Andini, Maydiana Ayu; Zahra, Safa Auli
Journal of Biotechnology and Natural Science Vol. 3 No. 2 (2023): December
Publisher : Universitas Ahmad Dahlan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.12928/jbns.v3i2.9502

Abstract

Abrasion of vehicle tires produced on highways on the imaginary line of Yogyakarta from Bantul up to Sleman Regency with different elevations has the potential to be a source of microplastics in the air and can contaminate rainwater. This study aims to determine the abundance and characteristics of microplastics in rainwater that falls on highways with different elevations in Yogyakarta Province. The study began with determining the sampling point and taking rainwater samples on the highway around Bantul Market, Tugu Monument, and Kaliurang km 14. Eight samples on different days were taken at each sampling point, as much as 250 mL/sample. Rainwater samples were filtered, microplastic abundance was calculated (particles/L), and grouped based on the shape, color, size, and polymer type. The content of microplastics on highways at different elevations in Yogyakarta Province was not significantly different, with an abundance range ranging from 200 to 484 particles/L. Vehicle density, light intensity, air temperature, and wind speed correlated with the abundance of microplastics in rainwater. The dominant characteristic is black fiber, 101-500 µm, and polyisoprene. Microplastics in rainwater that fell on the highways of Yogyakarta Province with varying elevations did not have different abundances but had the same characteristics.
The Effectiveness of Solid Waste Extract from Lemongrass (Cymbopogon citratus) as a Bioinsecticide for Controlling Whitefly (Bemisia tabaci) Pests on Pomelo Plants Tristiyanti, Alvia Sefie; Hayani, Alfitra; Ardianto, Sandi Dimas; Kurniasari, Laeli
Journal of Biotechnology and Natural Science Vol. 3 No. 2 (2023): December
Publisher : Universitas Ahmad Dahlan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.12928/jbns.v3i2.9669

Abstract

This research aims to analyze the effect of lemongrass waste extract on the mortality of whitefly and determine the optimal concentration of lemongrass waste extract to eradicate whiteflies within 24 hours. Lemongrass waste extract (leaves and stems) can be obtained through extraction using maceration. The waste is dried and ground to 60 mesh, then dissolved in 96% ethanol and ethyl acetate solvents at a ratio of 1:8 (w/v). Extraction is carried out over variable times of 2, 4, and 6 days. The optimal results are then used to create concentrations by dissolving 250 ml of distilled water in the mother solution, resulting in test solutions of 0, 200, 400, 800, and 1200 mg/L. Observations on the treatments are conducted after 24 hours, and the number of dead whiteflies is counted. Subsequently, the mortality of the whiteflies is analyzed. Based on the research findings, the highest mortality percentage after 24 hours is observed in the ethanol extract, with a dose of 1200 mg/L at 81.81%. After determining the mortality rates, probit analysis is conducted to determine the LC50 (Lethal Concentration 50) value. The optimal concentration for ethanol extract is found to be 1200 mg/L, with an LC50 of 885.102 mg/L. This indicates that lemongrass waste extract is moderately toxic to whiteflies.
Antagonistic Activities of Endophytic Fungi from Chili Pepper (Capsicum frutescens L.) Stems and Leaves against Colletotrichum sp. Puspitarini, Sofia; Aji, Oktira Roka
Journal of Biotechnology and Natural Science Vol. 3 No. 2 (2023): December
Publisher : Universitas Ahmad Dahlan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.12928/jbns.v3i2.9736

Abstract

Colletotrichum sp. is one of the type pathogenic fumgi that can cause antachnose diseases. One of the plants that can be attacked by antrachnose is cayenne pepper (Capsicum frutescens L.). This study attempts to determine the ability of endophytic fungi from the stems and leaves cayenne pepper (Capsicum frutescens L.). Methods: This study used dual culture method to determine the percentage of resistence to patogen Colletotrichum sp. and the area of growth endophytic fungi planted on PDA (Potato Dextrose Agar). Results:. There are 3 isolates from the stems and leaves of cayenne pepper that is JE-B4-C, JE-D5-C, dan JE-B1-C. Among all the isolates, the largest percentage of inhibition and growth area endophytic fungi were found in isolate JE-B4-C with each of 29,41% dan 58,04 cm2. Conclusions: Expected isolates JE-B4-C derived from the genus eupenicillium, while isolates JE-D5-C and JE-B1-C respectively derived from the genus Aspergillus and Neoscytalidium.
The Potential of Salvinia molesta as a Copper Phytoremediation Agent based on Gene Expression Analysis Astuti, Sutan Nur Chamida Tri; Solihah, Jumailatus; Aisah, Siti
Journal of Biotechnology and Natural Science Vol. 3 No. 1 (2023): June
Publisher : Universitas Ahmad Dahlan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.12928/jbns.v3i1.9739

Abstract

This research explores the rapid environmental impact of the batik industry, particularly concerning batik waste pollution, especially from the heavy metal copper (Cu). Untreated batik waste can have adverse effects on the environment. The study highlights the potential of the water fern plant (Salvinia molesta) as a heavy metal phytoremediator, specifically for Cu, by activating phytochelatin synthase. The research aims to determine the most effective waste concentration for Cu absorption by water ferns, measure the reduction in Cu levels after phytoremediation treatment, evaluate post-phytoremediation water quality, and observe PCS gene expression in the roots and leaves of water ferns. A static method is employed with variations in waste concentrations. Data analysis utilizes one-way ANOVA for Cu level reduction and changes in water quality. The results indicate that the most effective wastewater concentration for absorbing heavy metal copper (Cu) using water fern (Salvinia molesta) is 2%. The highest reduction in heavy metal copper (Cu) concentration after phytoremediation treatment with water fern (Salvinia molesta) is 41.48%. Water quality post-phytoremediation treatment using water fern (Salvinia molesta) at all concentrations exhibits improvement with an increase in dissolved oxygen (DO) levels. The expression of the phytochelatin synthase (PCS) gene in the leaves and roots of water fern (Salvinia molesta) confirms the role of the PCS gene in binding heavy metal copper (Cu) to the plant's vacuole.
Macrofungi Diversity in Wisdom Park UGM, Yogyakarta Zahra, Safa Auli; Safitri, Salsabila; Nugraha, Farhan Satya; Hidayah, Hidayah; Aji, Oktira Roka
Journal of Biotechnology and Natural Science Vol. 3 No. 2 (2023): December
Publisher : Universitas Ahmad Dahlan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.12928/jbns.v3i2.9743

Abstract

Indonesia's warm and humid tropical climate provides an ideal setting for the flourishing of fungi. Fungi are pivotal in the intricate balance of nutrient cycles and soil fertility within forest ecosystems. They can be broadly categorized into two types: microscopic fungi, which are minuscule and require observation under a microscope, and macroscopic fungi, which can be identified based on visible morphological features without additional tools. Forests, with their limited sunlight and high humidity due to the dense canopy of trees, serve as fertile grounds for a myriad of fungal species. This research aims to analyze fungal diversity using the exploratory survey method, involving direct search and observation of macrofungi. The sampling was conducted in May 2023 within the Wisdom Park UGM area, covering an area of 3,762.12 m² with coordinates 7° 46'09"S 110°22'59"E. The findings revealed a commendable diversity in terms of shape and size. However, the color palette of fungi in the area exhibited limited variation, primarily consisting of subdued white and brown hues. Some identified fungal species include Ganoderma sp., Mycena sp., Trametes sp., Lepiota cristata, and Panaeolus fimicola. These fungi predominantly thrived on substrates such as trees, soil, leaf litter, and decaying wood.

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