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Journal of Biodiversity and Biotechnology
Published by Universitas Sebelas Maret
ISSN : 27755223     EISSN : 28083229     DOI : https://doi.org/10.20961/jbb
Core Subject : Agriculture,
Agriculture, Biological Sciences & Forestry
Journal of Biodiversity and Biotechnology is a scientific journal focused on biodiversity and biotechnology published twice a year (June and December) managed and published by Universitas Sebelas Maret. This journal covers original research in the following areas: biodiversity and environment, biotechnology, agriculture biotechnology, food biotechnology, medical biotechnology, bioinformatics, molecular biology, biochemistry, bioinformatics, phyto-pharmacy, herbal medicine, and animal science. Other topics are welcome if it discusses about biodiversity or biotechnology.
Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Agronomi dan Ilmu Tanaman
Articles 31 Documents
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ANALYSIS OF CHARACTERISTICS AND LEVEL OF EFFICIENCY ON THE DEVELOPMENT OF ORGANIC RICE (Oryza Sativa, L.) FARMING BUSINESS IN BOYOLALI REGENCY suswadi suswadi; Agung Prasetyo
Journal of Biodiversity and Biotechnology Vol 1, No 2 (2021)
Publisher : Pusat Penelitian dan Pengembangan Bioteknologi dan Biodiversitas (P3BB) LPPM UNS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/jbb.v1i2.56424

Abstract

Certified organic agriculture is a product that has competitiveness and opportunities for export. This study aims to determine and analyze the characteristics of farmers, the total cost and revenue and income of organic rice, ze the level of efficiency, and BEP (Break-Even Point) of certified organic rice farming. The sampling method used in this study was simple random sampling with a sample of 40 farmers calculated using the Slovin formula. The analysis used is: (1) the analysis of the mean score for the characteristics of the farmers, (2) the method of analyzing costs and revenues and farm income, and (3) the analysis of the efficiency of the farming business using the R/C (Return Cost Ratio) analysis method. The research location was determined purposively, namely Dlingo Village, Mojosongo District, Boyolali Regency. The results of the study concluded that: (1) The characteristics of organic farmers were that men are more involved than women, the age of organic farming business practicioners were above 50 years old, most of the farmers in Dlingo village have lived for more than 30 years, the main livelihood farmers in the agricultural sector. (2) Based on the total average cost for organic farming, the certified group has an average of Rp.20,495,000,- and The average revenue was Rp. 35,000,000,-. The income per planting season is Rp. 14.505.000,-. Organic certified farmer groups get an R/C ratio value of 1.7;  the R/C > 1 means that farming with certified organic systems is efficient. Rice farming with a certified organic system is profitable because the BEP value of production volume was 2,927 < farmers' production, while the BEP value for production price was 4,099 < selling price at the farmer level.Keywords: organic rice; income; efficiency;
Risk factors for H9N2 contamination in live bird markets of the Greater Jakarta, Indonesia Rahma Hani Aisah; Bambang Sumiarto; Hendra Wibawa; Widagdo Sri Nugroho; Aprizal Panus; Sodirun Sodirun
Journal of Biodiversity and Biotechnology Vol 3, No 1 (2023)
Publisher : Pusat Penelitian dan Pengembangan Bioteknologi dan Biodiversitas (P3BB) LPPM UNS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/jbb.v3i1.74263

Abstract

Low Pathogenicity Avian Influenza virus subtype H9N2 causes decreased egg production and two of the four defined H9N2 poultry lineages (G1 and Y280) have been associated with human infections. The high positive test results for the H9N2 virus in the LBMs of the Greater Jakarta area in 2021 can be a source of transmission for the surrounding area. This study aims to determine the prevalence and risk factors that play a role in the H9N2 contamination in the LBMs to obtain a multivariate analysis model. A cross-sectional study was conducted on 87 vendors and 124 broiler chickens from 44 LBMs randomly selected in Tangerang City, North Jakarta, and Bekasi City. Samples were taken as equipment swabs at the stall of poultry carcass vendors, tracheal swabs, and cloacal swabs of broiler chicken. The test used was Real-Time Reverse Transcription Polymerase Chain Reaction. Data analysis was performed using univariate, bivariate, and multivariate analysis with logistic regression. The prevalence of H9N2 virus contamination at the LBM level in Greater Jakarta in 2022 was 77.27% (95% CI, α 7%), while the prevalence at the vendor level was 54.02% (95% CI, α 5%). The risk factors for H9N2 virus contamination with a significant p-value<0.05 include the positive results on equipment swab samples, positive results on both equipment and broiler swabs, and the use of carcass sink.  The multivariate analysis model of H9N2 virus contamination at the vendor level was Logit P (H9N2=1 | x) = 0.29924 - 2.42176PPE + 1.58691carcass sink. The risk factor contributing to increase H9N2 contamination is using carcass sinks, while PPE is the one that reduces H9N2 contamination.
Root Growth Response of Soybean Under Water Deficit Achmad Fatchul Aziez; Agung Prasetyo; Paiman Paiman
Journal of Biodiversity and Biotechnology Vol 2, No 2 (2022)
Publisher : Pusat Penelitian dan Pengembangan Bioteknologi dan Biodiversitas (P3BB) LPPM UNS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/jbb.v2i2.66465

Abstract

Roots are plant organs that function to absorb water and nutrients from the rhizosphere. If the soil is dry, the roots will be affected first. This study aims to know the response of soybean root to drought stress. This research was arranged in a randomized completely block design (RCBD) with two factors and three replications. The first factor was soil moisture content, which consisted of four levels, e.i., 100, 75, 50, and 25% field capacity. The second factor was the growth stage, which consisted of three kinds, e.i., the vegetative active, flowering time, and seed filling period. The results showed that the soil water content in below 75% field capacity decreased root length, root fresh weight, root dry weight, root volume, and increased shoot root ratio. The seed filling period was more sensitive to water deficiency than the active vegetative and flowering time. The study findings that soybean plants can grow well at 100% field capacity. The practical implication of planting soybeans use a soil moisture content of 100% field capacity.
Genetic of Salak Pondoh, Gading Varieties and Its Hybrids Based on RAPD Markers Nandariyah Nandariyah; Parjanto Parjanto; Pinaka Pinasti Ratu
Journal of Biodiversity and Biotechnology Vol 1, No 1 (2021)
Publisher : Pusat Penelitian dan Pengembangan Bioteknologi dan Biodiversitas (P3BB) LPPM UNS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/jbb.v1i1.50396

Abstract

A molecular marker of parent and offspring is used to find fast and accurate markers influenced by DNA isolation and amplification. This research aims to find the most suitable DNA isolation and DNA  amplification methods. This study used four DNA isolation methods; namely IM01, IM02, IM03, and IM04. DNA amplification used ten protocols (AP01, AP02, AP03, AP04, AP05, AP06, AP07, AP08, AP09, and AP010). The results of the research showed that the most suitable DNA isolation method for salak was  IM0, and the most suitable DNA amplification for salak was AP04 that produces the highest value of DNA bands. Keywords: DNA isolation; DNA amplification; hybrids.
DIVERSITY OF SAPODILLA (ACHRAS ZAPOTA L.) GROWTH FROM WONOGIRI AND BOJONEGORO IN DIFFERENT AGE OF TRANSPLANTATION SEED Endang Yuniastuti; Muhammad Agung; Parjanto Parjanto
Journal of Biodiversity and Biotechnology Vol 2, No 1 (2022)
Publisher : Pusat Penelitian dan Pengembangan Bioteknologi dan Biodiversitas (P3BB) LPPM UNS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/jbb.v2i1.61170

Abstract

Most farmers determine the cutting of air layer seed in a corp through the amount of the growing roots from air layer itself. The roots can be an indicator of air layer cutting method in a corp. The roots, which are too old or too young, grow less optimal in the air layer seed after being transferred into another cultivated medium. It is necessary to have knowledge about the precise age of air layer cutting so that the vegetative propagation by using air layer can be performed accurately and efficiently. The treatment that is executed is the age of air layer and the corps accession. The result of this research indicates that the effect of the difference air layer age on plant growth is at the age of emerging buds, aged 180 Day is treated with the age of the fastest emerging shoots. The growth of Bojonegoro air layer plants results that does not differ with Wonogiri air layer plants. There is no interaction between air layer age with sapodilla accession on plant growth.
MORPHOLOGICAL CHARACTERS OF EARLY HARVEST AGE MUTANT SELECTION M3 GENERATION OF BLACK RICE (ORYZA SATIVA L.) IRRADIATED BY GAMMA RAY Nandariyah - Nandariyah; Parjanto Parjanto; Sutarno Sutarno; Riyatun Riyatun; Rifqi Syarif Muhammad
Journal of Biodiversity and Biotechnology Vol 1, No 2 (2021)
Publisher : Pusat Penelitian dan Pengembangan Bioteknologi dan Biodiversitas (P3BB) LPPM UNS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/jbb.v%vi%i.56382

Abstract

Black rice contains higher levels of anthocyanin, fiber, and protein compared to local white rice. The price of black rice is higher compared to local white rice, it is one of prove that black rice has functional and economical values. The main obstacles in the cultivation of black rice are the long harvest age, high plant height, and lack of productivity. One way that can be applied to overcome this dissadvantageis with the utilization of gamma ray irradiation. The purpose of this research is to get the early-season rice, short stem, and higher productivity. This research was conducted in Tempel, Gatak, Sukoharjo District, by planting M3 seed (gamma ray irradiation of 300 Gray dosage on M2). The results of black rice from gamma ray irradiation of 300 gray dosage are M-CI-03-239, M-CI-03-244, M-CI-03-246, M-CI-03-346, M- CI-03-350, M-CI-03-234, M-CI-03-346, M-CI-03-350, M-CI-03-250, M-CI- 03-346, M-CI-03-CI-03-262, M-CI-03-265, M-CI-03-270, M-CI-03-283, and M-CI-03-311 with the agronomical properties of early age, i.e 2 weeks faster (64 days) with control (80 days). There are 125 short-postured plants of gamma ray irradiation result with  99.2% percentage. There are 12 individual plants in the M3 strain (M-CI-03-240, M-CI-03-251, and M-CI-03-261) of gamma ray irradiation with the maturation category, short stems, and high productivity compared with control. The difference of productivity of irradiated crops with control is 57,91%.
Epidemiology Study of Brucellosis in Polewali Mandar District, Indonesia Fitri Amaliah; Bambang Sumiarto; Widagdo Sri Nugroho; Tri Untari; Dinar Hadi Wahyu Hartawan; Agustia Agustia
Journal of Biodiversity and Biotechnology Vol 3, No 1 (2023)
Publisher : Pusat Penelitian dan Pengembangan Bioteknologi dan Biodiversitas (P3BB) LPPM UNS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/jbb.v3i1.74264

Abstract

This research aims to determine the prevalence rate of brucellosis in the Polman District, examine the risk factors associated with seropositive diagnoses of brucellosis, and also to determine the brucellosis case model at the farm level. This research was carried out through a cross-sectional study and obtained a sample size of 395 serum. Samples were tested through the RBT and CFT. Identification of risk factors is based on questionnaire data conducted on around 113 breeders spread across Polman Regency. The data were analyzed using univariate and bivariate descriptive statistics, chi-square, and odds ratio (OR). The brucellosis model at the farm level was analyzed by multivariate logistic regression. The results of this study indicate the prevalence of brucellosis in Polman Regency is 27.1%. Risk factors associated with diagnosis of brucellosis in Polman Regency included the history of abortion (p=0.000; OR=128), handling of aborted animal (p=0.000; OR=94.667), abortion at the end of pregnancy (p=0.000; OR=62.333), knowledge about brucellosis (p=0.000; OR=9.841), handling of residual abortions (p=0.003; OR=8,471), high grazing density (p=0.000; OR=6.861), introduction of new livestock (p=0.006; OR=5.833), dirty cage (p=0.003; OR=3.600), and grazing method (p=0.005; OR=3.167). Models of diagnosis brucellosis in Polman Regency is = -2,48923 + 3,38734 history of abortion + 2,06312 abortions at the end of pregnancy. The model shows that the history of abortion (β=+3.387; OR=29.588) and the incidence of abortion at the end of pregnancy (β=+2.205; OR = 7.871) can increase the seropositive diagnosis of brucellosis at the farm level.
The Use of Organic And Anorganic And Mulch On Zucchini (Cucurbita pepo L) Plant With Elephant Manure From Borobudur Tample Pramono Hadi; Firda Rizkian; Tri Rahayu
Journal of Biodiversity and Biotechnology Vol 1, No 2 (2021)
Publisher : Pusat Penelitian dan Pengembangan Bioteknologi dan Biodiversitas (P3BB) LPPM UNS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/jbb.v%vi%i.56292

Abstract

This study aims at identifying the use of organic and anorganic mulch of the zucchini plants appropriate elephant manure inyorder to obtain optimal growth and yield. The study has been on, Boyolali Regency with an altitude of 170 m above sea level.Thishresearch method is an experiment using a factorial treatment Randomized Completed Block Design (RCBD) with 3 times. There are two kinds of treatment under the study that is dose of elephant manure and kind of  mulch, elephant manure dose treatment factor (G), there are 3 levels of treatment, namely (G1) Elephant manure dose 5 tons / ha, (G2) Elephant manure dose 10 tons / ha, (G3) The dose of elephant manure is 15 tons / ha, the treatment factor is mulch (M), there are 3 types of treatment, namely (M0) without mulch (M1) Silver mulch (M2) mulch straw.Therresults of this study show that treatment for the dose of elephant manure (G) showed no significant different to all observed parameters, the mulch type treatment (M) show highly significant to fruituweightoperlplantmand fruit diameter, show significant to fruit length, and biomassyfreshpweight per plant, while biomasydry weight per plant showed no significant effect. The interaction between elephant manure dose and mulch type (GxM) showed no significant to all observed parameters. The highest fruit weight yield was (478.84 g) in the treatment combination and the lowest was obtained in the treatment combination (253.05 g).
ANTIBACTERIAL ACTIVITY AND TLC-BIOTOGRAPHY PROFILE OF THE ETHYL ACETAT FRACTION OF ASIAN PIGEONWINGS FLOWER (Clitoria ternatea) AGAINST Escherichia coli Ari Susilowati; Rizkya Suhardo Putri; Widya Mudyantini
Journal of Biodiversity and Biotechnology Vol 2, No 2 (2022)
Publisher : Pusat Penelitian dan Pengembangan Bioteknologi dan Biodiversitas (P3BB) LPPM UNS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/jbb.v2i2.66769

Abstract

Infectious diseases are diseases caused by the presence of microorganisms that enter and develop in the body, causing several clinical symptoms and signs. One of the infectious diseases is diarrhea caused by Escherichia coli. There are many potential herbal plants as alternative antibacterial antibiotics, one of which comes from the Asian pigeonwings flower (Clitoria ternatea). This study aims to determine the antibacterial activity of the ethyl acetate fraction of pigeonwings flower, phytochemical compounds and its TLC-bioautography profile. The antibacterial compound of pigeonwings flower was extracted by maceration using ethanol and then fractionated by ethyl acetate and made solutions with concentrations of 5, 10, 20, and 40%, respectively. The experiment was carried out with 3 replications using the well diffusion method and followed by TLC-bioautography to determine the TLC-bioautography profile. Phytochemical compounds of pigeonwings flower were determined by thin layer chromatography (TLC). Antibacterial activity data were analyzed using the One Way ANOVA and continued with the Tukey HSD 5% through SPSS. The ethyl acetate fraction of pigeonwings flower had the best antibacterial activity at a concentration of 40% with an inhibition zone diameter of 11.54 mm. The diameter of the inhibition zone showed significantly different in each concentration and it can be said that the ethyl acetate fraction of pigeonwings flower was able to inhibit the growth of E. coli. The phytochemical compounds contained in the ethyl acetate fraction of pigeonwings flower are flavonoids and alkaloids with flavonoids being the most active compounds in inhibiting the growth of E. coli.
Relationship of Personal Hygiene and Nutritional Status to Intestinal Parasitic Infection in Simo, Boyolali Sherlina Rintik Tirta Ayu; Sri Haryati; Yulia Sari; Sutartinah Handayani
Journal of Biodiversity and Biotechnology Vol 1, No 1 (2021)
Publisher : Pusat Penelitian dan Pengembangan Bioteknologi dan Biodiversitas (P3BB) LPPM UNS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/jbb.v1i1.50237

Abstract

Intestinal parasitic infections are caused by protozoa, STH and non-STH worms. Immunity, which can be determined by measuring nutritional status, and personal hygiene can affect the occurrence of intestinal parasite infections. This study aims to determine the relationship between personal hygiene and nutritional status against intestinal parasite infections in elementary school students in Simo Boyolali. This type of analytic observational research with a cross sectional approach. Total sampling was taken at elementary school of Wates, Talakbroto 1, and Kedunglengkong 1 Simo, Boyolali. There were 11 students with worms infections, 16 with protozoa infections, and 4 with worms and protozoa infection. There was a relationship between washing hands before eating (p = 0.004), after eating (0.027), after defecating (p = 0.04), biting nails (p = 0.008), wearing footwear when leaving the house (p = 0.008) , removing shoes while playing during school breaks (p = 0.001), and nutritional status (p = 0.002) with intestinal parasite infection. There was no relationship between nail clipping once a week (p = 0.118) and the availability of a latrine (p = 0.416) with intestinal parasitic infections. So, there is a relationship between personal hygiene (washing hands before eating, after eating, after defecating, biting nails, wearing footwear when leaving the house, removing shoes when playing during school breaks, and nutritional status) and intestinal parasitic infections. Keywords: elementary school; protozoa; worms.

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