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Journal of Biodiversity and Biotechnology
Published by Universitas Sebelas Maret
ISSN : 27755223     EISSN : 28083229     DOI : https://doi.org/10.20961/jbb
Core Subject : Agriculture,
Agriculture, Biological Sciences & Forestry
Journal of Biodiversity and Biotechnology is a scientific journal focused on biodiversity and biotechnology published twice a year (June and December) managed and published by Universitas Sebelas Maret. This journal covers original research in the following areas: biodiversity and environment, biotechnology, agriculture biotechnology, food biotechnology, medical biotechnology, bioinformatics, molecular biology, biochemistry, bioinformatics, phyto-pharmacy, herbal medicine, and animal science. Other topics are welcome if it discusses about biodiversity or biotechnology.
Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Agronomi dan Ilmu Tanaman
Articles 60 Documents
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Epidemiology Study of Brucellosis in Polewali Mandar District, Indonesia Fitri Amaliah; Bambang Sumiarto; Widagdo Sri Nugroho; Tri Untari; Dinar Hadi Wahyu Hartawan; Agustia Agustia
Journal of Biodiversity and Biotechnology Vol 3, No 1 (2023)
Publisher : Pusat Penelitian dan Pengembangan Bioteknologi dan Biodiversitas (P3BB) LPPM UNS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/jbb.v3i1.74264

Abstract

This research aims to determine the prevalence rate of brucellosis in the Polman District, examine the risk factors associated with seropositive diagnoses of brucellosis, and also to determine the brucellosis case model at the farm level. This research was carried out through a cross-sectional study and obtained a sample size of 395 serum. Samples were tested through the RBT and CFT. Identification of risk factors is based on questionnaire data conducted on around 113 breeders spread across Polman Regency. The data were analyzed using univariate and bivariate descriptive statistics, chi-square, and odds ratio (OR). The brucellosis model at the farm level was analyzed by multivariate logistic regression. The results of this study indicate the prevalence of brucellosis in Polman Regency is 27.1%. Risk factors associated with diagnosis of brucellosis in Polman Regency included the history of abortion (p=0.000; OR=128), handling of aborted animal (p=0.000; OR=94.667), abortion at the end of pregnancy (p=0.000; OR=62.333), knowledge about brucellosis (p=0.000; OR=9.841), handling of residual abortions (p=0.003; OR=8,471), high grazing density (p=0.000; OR=6.861), introduction of new livestock (p=0.006; OR=5.833), dirty cage (p=0.003; OR=3.600), and grazing method (p=0.005; OR=3.167). Models of diagnosis brucellosis in Polman Regency is = -2,48923 + 3,38734 history of abortion + 2,06312 abortions at the end of pregnancy. The model shows that the history of abortion (β=+3.387; OR=29.588) and the incidence of abortion at the end of pregnancy (β=+2.205; OR = 7.871) can increase the seropositive diagnosis of brucellosis at the farm level.
The Use of Organic and Anorganic and Mulch on Zucchini (Cucurbita pepo L) Plant with Elephant Manure from Borobudur Tample Pramono Hadi; Firda Rizkian; Tri Rahayu
Journal of Biodiversity and Biotechnology Vol 1, No 2 (2021)
Publisher : Pusat Penelitian dan Pengembangan Bioteknologi dan Biodiversitas (P3BB) LPPM UNS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/jbb.v%vi%i.56292

Abstract

This study aims at identifying the use of organic and anorganic mulch of the zucchini plants appropriate elephant manure inyorder to obtain optimal growth and yield. The study has been on, Boyolali Regency with an altitude of 170 m above sea level.Thishresearch method is an experiment using a factorial treatment Randomized Completed Block Design (RCBD) with 3 times. There are two kinds of treatment under the study that is dose of elephant manure and kind of  mulch, elephant manure dose treatment factor (G), there are 3 levels of treatment, namely (G1) Elephant manure dose 5 tons / ha, (G2) Elephant manure dose 10 tons / ha, (G3) The dose of elephant manure is 15 tons / ha, the treatment factor is mulch (M), there are 3 types of treatment, namely (M0) without mulch (M1) Silver mulch (M2) mulch straw.Therresults of this study show that treatment for the dose of elephant manure (G) showed no significant different to all observed parameters, the mulch type treatment (M) show highly significant to fruituweightoperlplantmand fruit diameter, show significant to fruit length, and biomassyfreshpweight per plant, while biomasydry weight per plant showed no significant effect. The interaction between elephant manure dose and mulch type (GxM) showed no significant to all observed parameters. The highest fruit weight yield was (478.84 g) in the treatment combination and the lowest was obtained in the treatment combination (253.05 g).
Antibacterial Activity and Tlc-Biotography Profile of the Ethyl Acetat Fraction of Asian Pigeonwings Flower (Clitoria ternatea) against Escherichia coli Ari Susilowati; Rizkya Suhardo Putri; Widya Mudyantini
Journal of Biodiversity and Biotechnology Vol 2, No 2 (2022)
Publisher : Pusat Penelitian dan Pengembangan Bioteknologi dan Biodiversitas (P3BB) LPPM UNS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/jbb.v2i2.66769

Abstract

Infectious diseases are diseases caused by the presence of microorganisms that enter and develop in the body, causing several clinical symptoms and signs. One of the infectious diseases is diarrhea caused by Escherichia coli. There are many potential herbal plants as alternative antibacterial antibiotics, one of which comes from the Asian pigeonwings flower (Clitoria ternatea). This study aims to determine the antibacterial activity of the ethyl acetate fraction of pigeonwings flower, phytochemical compounds and its TLC-bioautography profile. The antibacterial compound of pigeonwings flower was extracted by maceration using ethanol and then fractionated by ethyl acetate and made solutions with concentrations of 5, 10, 20, and 40%, respectively. The experiment was carried out with 3 replications using the well diffusion method and followed by TLC-bioautography to determine the TLC-bioautography profile. Phytochemical compounds of pigeonwings flower were determined by thin layer chromatography (TLC). Antibacterial activity data were analyzed using the One Way ANOVA and continued with the Tukey HSD 5% through SPSS. The ethyl acetate fraction of pigeonwings flower had the best antibacterial activity at a concentration of 40% with an inhibition zone diameter of 11.54 mm. The diameter of the inhibition zone showed significantly different in each concentration and it can be said that the ethyl acetate fraction of pigeonwings flower was able to inhibit the growth of E. coli. The phytochemical compounds contained in the ethyl acetate fraction of pigeonwings flower are flavonoids and alkaloids with flavonoids being the most active compounds in inhibiting the growth of E. coli.
Antibacterial Activity of Chayote (Sechium edule Swartz) Squash Extracts and Their Phytochemical Constituents Nur Indah Sulistiyani; Ahmad Ainurrofiq; Venty Suryanti
Journal of Biodiversity and Biotechnology Vol 2, No 1 (2022)
Publisher : Pusat Penelitian dan Pengembangan Bioteknologi dan Biodiversitas (P3BB) LPPM UNS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/jbb.v2i1.61330

Abstract

Antibacterial activity of chayote (Sechium edule Swartz) squash extracts against pathogenic bacteria has been evaluated. Dried chayote squash powder was extracted using methanol which was then extracted successively using hexane, chloroform, ethyl acetate, and butanol. The antibacterial activities of the extracts were tested by the hole diffusion method. Methanol extract of chayote squash had antibacterial activity against Staphylococcus aureus, Bacillus subtilis, Pseudomonas aeruginosa, and Escherichia coli but did not have antibacterial activity against Enterobacter aerogenes, Shigella dysenteriae, and Salmonella typhi. Ethyl acetate extract had the highest antibacterial activity against P. aeruginosa and E. coli. The ethyl acetate extract contains phenolics, condensed tannins, flavonoids, and terpenoids. The ethyl acetate extract had a MIC of 50 mg/ml for S. aureus, B. subtilis, P. aeruginosa, and E. coli. Different bioactivities of tested extracts were found due to various chemical compositions of extracts as a result of different polarities of the solvent.
Effect of Auxin and Cytokinin on Orchid Subculture Resulting from Coelogyne Crosses Sri Hartati; Ahmad Yunus; Iistikharoh Iistikharoh
Journal of Biodiversity and Biotechnology Vol 3, No 2 (2023)
Publisher : Pusat Penelitian dan Pengembangan Bioteknologi dan Biodiversitas (P3BB) LPPM UNS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/jbb.v3i2.80626

Abstract

The black orchid or Coelogyne is one of the native orchids of Kalimantan, widely cultivated in Indonesia. Several species of Coelogyne orchids have been crossed to obtain new superior-quality plants. Modifying the media, such as giving PGR, is necessary for good plant multiplication results. This research was conducted by changing MS growing media with ZPT BA and NAA. This experiment aimed to determine the effect of ZPT BA and NAA on the growth of orchid subcultures from a cross between Coelogyne pandhurata and Coelogyne rumphii. The study used a completely randomized design (RAL) with 2 factors, namely ZPT BA and NAA, each having a concentration of 0 ppm, 0.2 ppm; 0.4 ppm, and 0.6 ppm with 16 treatment combinations and 4 replications. They were implemented at the Laboratory Plant Physiology and Biotechnology, Faculty of Agriculture, UNS Surakarta, from November 2020 to March 2021. The variables observed included the number of shoots, plant length, plant weight, number of leaves, number of roots, root length, when roots appeared, and when shoots appeared. The results showed that BA 0.6 ppm gives the best output on the growth of the number of shoots, and NAA concentration of 0,2 ppm gives the best results on the variable number of roots. The combination of BA 0.6 ppm and NAA 0.2 ppm gave the best results on plant weight growth.
Morphological Character of The Natural Orchid Phalaenopsis Spp. Results of Colchicine Polyploidy Induction Sri Hartati; Samanhudi Samanhudi; Endang Suprapti
Journal of Biodiversity and Biotechnology Vol 4, No 2 (2024)
Publisher : Pusat Penelitian dan Pengembangan Bioteknologi dan Biodiversitas (P3BB) LPPM UNS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/jbb.v4i2.108518

Abstract

Induction of polyploidy in orchids can cause morphological changes such as larger leaf and flower sizes, longer bloom durations, and sharper flower colors. Morphological characterization needs to be done to determine the morphological changes that occur after colchicine treatment. This study aims to determine the morphological characters of natural orchids Phalaenopsis spp. polyploidy induction using colchicine. The research was carried out from November 2021-February 2022 at the Screen House, Faculty of Agriculture, UNS. The plant materials used included P. violacea from Sulawesi, P. violacea from Java, and P. Ambilis from Ambon. The treatment used was colchicine 1,250 ppm which was applied to flower buds using the cotton method with 2 replications. The collected data were analyzed by descriptive analysis and paired T-test using IBM SPSS Statistics 26 to compare the average performance of mutant plants with control plants. Induction of polyploidy using colchicine with a concentration of 1,250 ppm could increase the size of leaves, flower stalks, flowers, dorsal and lateral sepals, and petals in P. violacea from Java, P. violacea from Sulawesi, and P. amabilis from Ambon.
Morphological Characterization of Matoa the “Permata Papua” Fruit (Pometia pinnata) in Central Java Endang Yuniastuti; ML Samodra; Muji Rahayu; Iswahyudi Iswahyudi
Journal of Biodiversity and Biotechnology Vol 4, No 1 (2024)
Publisher : Pusat Penelitian dan Pengembangan Bioteknologi dan Biodiversitas (P3BB) LPPM UNS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/jbb.v4i1.107921

Abstract

This research aims to obtain morphological character of Matoa (Pometia pinnata JR Forst & G Forst) plant. This research had been conducted from April to October 2021, in 5 Regency (Karanganyar, Klaten, Pati, Sukoharjo, and Surakarta) Central Java. The morphological characther based on direct observation through primary and secondary data recording, and documenting parts of an adult matoa plant with purposive random sampling. Data of morphological characterization of matoa was analyzed using descriptive analysis method with assistance of Ntsys v2.02i program. The results showed a morphological diversity of matoa plant accessions. Fifty (50) matoa accessions exhibit morphological diversity in plant height, canopy diameter, stem circumference, branching type, leaf colour, number of leaflets, compound leaf length, compound leaf petiole length, leaflet width, fruit density, fruit length, fruit weight, seed weight, and fruit skin colour.
Phenology Dynamics: Flowering and Fruit Development of Prabu Bestari Grape Variety in Denpasar Ni Ketut Karina Sari; Dewa Ayu Ari Ari Febriayanti; Ni Nyoman Ari Putri Murtiyah; Dicky Marsadi
Journal of Biodiversity and Biotechnology Vol 5, No 2 (2025)
Publisher : Pusat Penelitian dan Pengembangan Bioteknologi dan Biodiversitas (P3BB) LPPM UNS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/jbb.v5i2.109980

Abstract

Grape (Vitis vinifera L.) var. Prabu Bestari is an important fruit commodity, but information regarding its reproductive phenology is still minimal. This qualitative descriptive study aimed to determine the time periods required for key phenological stages, namely bud burst, bloom, fruit set, and fruit ripening (harvest), which are crucial for breeding and optimizing production. The research was conducted at the Faculty of Agriculture's Experimental Garden (0–9 masl). The observations revealed a rapid reproductive growth cycle. The average time from pruning to shoot emergence (bud burst) was 7 days after pruning (dap). The phase from bud burst to flowering lasted 18–19 days. Full bloom was very brief, lasting only 1 day, and the phase from flowering to fruit set required 1–3 days. The longest period was from fruit set to harvest, lasting 48 days. Overall, the cycle from pruning to harvest for the Prabu Bestari variety required an average of 120 dap. This phenological data provides an essential basis for effective cultivation management.
Valuing And Characterization of Smallholder’s Forest In West Java Endang Hernawan; Tien Lastini; Mia Rosmiati
Journal of Biodiversity and Biotechnology Vol 4, No 1 (2024)
Publisher : Pusat Penelitian dan Pengembangan Bioteknologi dan Biodiversitas (P3BB) LPPM UNS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/jbb.v4i1.81489

Abstract

Today, smallholder forests in West Java - Indonesia play an important role as suppliers of domestic and industrial wood needs. However, with forest managers who are not yet professional and the easy conversion of land use into non-forest, smallholder forests cannot guarantee the sustainability of wood supply. In supporting the implementation of sustainable forest management in smallholder forests, understanding is needed regarding the characteristics of smallholder forests. The purpose of this study is to evaluate the characteristics of smallholder forest stands as a basis for assessing the sustainability of wood raw material supply. The research location is smallholder forest in Sumedang district. Data collection method is a purposive sampling method with moving plot. The results indicated that the density of smallholder forests per ha was 342.5 trees per ha, with 14 species of trees. The distribution of tree diameters ranges from 1-49 cm, with a predominant class diameter of 10-15 cm. The potential stands for smallholder forests are 32.68 m3ha-1. The cutting felling cycle is at the age of 6.5 years with a selective logging system at a diameter size of more than 15 cm, so it is not optimal and there is a tendency that it will not continue. Therefore, in increasing its sustainability, it is necessary to increase the cutting life. So, there is a tendency for community forest areas to be converted into other land uses.
Role of Organic and Phosphate Fertilizer on Growth and Yield of Groundnut Supriyono Supriyono; Cholis Laila Aryani; Pardono Pardono; Maria Theresia Sri Budiastuti
Journal of Biodiversity and Biotechnology Vol 5, No 1 (2025)
Publisher : Pusat Penelitian dan Pengembangan Bioteknologi dan Biodiversitas (P3BB) LPPM UNS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/jbb.v5i1.111404

Abstract

Efforts to increase groundnut productivity can be achieved by fertilizing with organic and phosphate fertilizers. This research aims to examine the role of organic and phosphate fertilizers on growth and yield of groundnut. The research was conducted at the Faculty of Agriculture UNS Jumantono, EMPT (Ecology and Plant Production Management) Laboratory, Soil Chemistry and Fertility Laboratory from December 2024 to March 2025. The research used a Randomized Complete Block Design (RCBD) with two factors and each repeated three times. The first factor is dose of organic cow manure fertilizer (0, 10, 20, 30 ton.ha-1) and second factor is dose of phosphate fertilizer (0, 75, 150, 225 kg.ha-1). The observed variables include initial soil analysis, growth, yield, plant tissue analysis, and nutrient uptake analysis. Observational data were analyzed using analysis of variance (ANOVA) at a 95% confidence significant, Duncan Multiple Range Test (DMRT) at a 95% confidence significant, regression tests, and Pearson correlation tests. The results showed that cow manure fertilizer with an optimum dose of 25.67 ton.ha-1 combined with 225 kg.ha-1 of phosphate fertilizer resulted in the early flowering age, namely 24.97 days after planting. Organic cow manure fertilizer 10 ton.ha-1 was able to increase the number of leaves, leaf area index, fresh weight of straw, number of pods, dry weight of pods, number of seeds per plant, and seed weight per plant of groundnut compared without organic cow manure fertilizer. Phosphate fertilizer 75 kg.ha-1 was able to increase the number of pods, dry weight of pods, and number of seeds per plant compared without phosphate fertilizer.

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