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Journal of Biodiversity and Biotechnology
Published by Universitas Sebelas Maret
ISSN : 27755223     EISSN : 28083229     DOI : https://doi.org/10.20961/jbb
Core Subject : Agriculture,
Agriculture, Biological Sciences & Forestry
Journal of Biodiversity and Biotechnology is a scientific journal focused on biodiversity and biotechnology published twice a year (June and December) managed and published by Universitas Sebelas Maret. This journal covers original research in the following areas: biodiversity and environment, biotechnology, agriculture biotechnology, food biotechnology, medical biotechnology, bioinformatics, molecular biology, biochemistry, bioinformatics, phyto-pharmacy, herbal medicine, and animal science. Other topics are welcome if it discusses about biodiversity or biotechnology.
Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Agronomi dan Ilmu Tanaman
Articles 31 Documents
 1   2   3   4 
Effect of gamma irradiation on total phenolic contents and antioxidant activities of methanolic extract of java plum seeds Rohadi Rohadi; Iswoyo Iswoyo; Dewi Larasati; Sugili Putra
Journal of Biodiversity and Biotechnology Vol 3, No 1 (2023)
Publisher : Pusat Penelitian dan Pengembangan Bioteknologi dan Biodiversitas (P3BB) LPPM UNS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/jbb.v3i1.71721

Abstract

The synthetic antioxidant butylated hydroxyanisole (BHA) has been widely added to lipids and food products as a preservative due to inhibiting lipid oxidation. However, concerns have arisen about the potential health impacts of this synthetic antioxidant on consumers. Therefore, there is a need for an alternative substitute for BHA that is safe for human health. The methanolic extract of Java Plum seeds (MEJS) contains a diverse group of phenolic compounds and has the potential to serve as a natural antioxidant. The antioxidant must remain stable during food processing, such as irradiation. This research aimed to determine the effect of γ-irradiation on the total phenolic content and antioxidant activity of MEJS. When γ-irradiation was applied to MEJS at doses up to 12.5 kGy, there was a slight decrease in total phenolic and tannin content but a significant increase in total flavonoid content. This increase in total flavonoids led to a rise in both free radical scavenging and reducing power activities
Vitamin E and Antioxidants Content of Portulaca oleracea L. Form Various Altitudes in East Java, Indonesia F Deru Dewanti; Bambang Pujiasmanto; Sukendah Sukendah; Ahmad Yunus
Journal of Biodiversity and Biotechnology Vol 1, No 1 (2021)
Publisher : Pusat Penelitian dan Pengembangan Bioteknologi dan Biodiversitas (P3BB) LPPM UNS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/jbb.v1i1.50384

Abstract

This study aims to obtain purslane plants as a source of local food and a quality bioactive component from various altitudes in East Java, Indonesia. Purslane planting materials were collected from the lowlands <200 m asl in Surabaya, medium plains 200-800 m asl in Malang DAU, and highlands> 800 m asl in Batu, Malang. Analysis of vitamin E was as total tocopherol. The results of the analysis of the content of Portulaca oleraceae L. of vitamin E were as followed: in the highlands 0.1056%, medium lands 0.1253%, and lowlands 1.162%. The analysis showed that Portulaca oleraceae L. could be a source of high quality local food and bioactive components because it contains vitamin E and antioxidants.Keywords: bioactive components; local food; lowland. 
Application of Livestock Waste Liquid Fertilizer and Mychorriza Biofertilizer on The Growth of Biduri (Calotropis gigantea) Muhammad Naufal Adani; Samanhudi Samanhudi; Amalia Tetrani Sakya; Sri Hartati; Muji Rahayu; Anne Sukmawati
Journal of Biodiversity and Biotechnology Vol 2, No 2 (2022)
Publisher : Pusat Penelitian dan Pengembangan Bioteknologi dan Biodiversitas (P3BB) LPPM UNS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/jbb.v2i2.63262

Abstract

Biduri (Calotropis gigantea) was a plant with several benefits, namely as a medicinal plant and textile material. Biduri was a wild plant that could grow in various types of soil conditions. The existence of biduri was still not fully utilized by many people. So it was necessary to develop the cultivation technology of Biduri to take advantage of this plant. This research was conducted at the field laboratory of Jumantono, Faculty of Agriculture, Universitas Sebelas Maret, Karanganyar, and used a factorial Randomized Complete Block Design (RCBD), which consisted of 2 factors, namely organic liquid fertilizer from livestock waste and mycorrhizae. Livestock waste liquid fertilizer consists of 40ml/kg soil cow urine, 40ml/kg soil goat urine, 40ml/kg soil rabbit urine, and 40ml/kg soil fish waste. Mycorrhizae consisted of 0 grams/plant, 5 grams/plant, 10gram/plant, and 15gram/plant. There were 20 treatment combinations repeated 3 times, so the total was 60 treatment combinations. The interaction between livestock waste liquid fertilizer and mycorrhizae did not significantly affect all the physiological observation variables. The results showed that the application of livestock waste liquid fertilizer had no significant effect on all observations of physiological variables. Giving mycorrhizae shows that it can increase the number of stomata and the width of stomata aperture.
Antibacterial Activity Test of Ethanol Extract and SAP of Betung Bamboo Shoot (Dendrocalamus asper) Against Klebsiella pneumoniae and Pseudomonas aeruginosa Bacteria Anif Nur Artanti; Farikatul Sufi Mujahidah
Journal of Biodiversity and Biotechnology Vol 1, No 1 (2021)
Publisher : Pusat Penelitian dan Pengembangan Bioteknologi dan Biodiversitas (P3BB) LPPM UNS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/jbb.v1i1.50519

Abstract

Boiled water from bamboo shoots is often used for pneumonia and a cleanser for the wound. It is known that bamboo can be an antibacterial agent because it has saponin, alkaloid, and flavonoid. This research aims to study whether ethanol extract and sap from bamboo betung shoot (Dendrocalamus asper) has secondary metabolism and antibacterial activity against Klebsiella pneumoniae and Pseudomonas aeruginosa. The extract was taken by maceration method using ethanol 96%, and the sap was taken from the water of bamboo shoot. Antibacterial activity test was conducted using disc diffusion method to measure the magnitude of inhibitory power at six concentration ratios of b/v solutions (10%; 20%; 30%; 40%; 50%; 60%). The result showed that ethanol extract and sap of bamboo betung shoot contained saponin, quercetin, and quinine which were supposed to have antibacterial activity against Klebsiella pneumoniae and Pseudomonas aeruginosa. The largest inhibitory zone diameter was found in ethanol extract 60% with the category of moderate inhibition of 9.05 ± 0.12 mm against Kliebsiella pneumoniae and 5.07 ± 0.13 mm against Pseudomonas aeruginosa. While the sap with a concentration of 60% was included in the weak inhibitory category, which was 5.65 ± 0.05 mm in the Kliebsiella pneumoniae and 4.81 ± 0.22 mm in Pseudomonas aeruginosa. Keywords: antibacterial; bamboo shoot; betung bamboo.
The Effect of Dosage and Frequency of Leri Water Spraying on Phalaenopsis Fuller's Pink Stripe Orchid Growth of Aclimatisation Sri Hartati; Ongko Cahyono
Journal of Biodiversity and Biotechnology Vol 2, No 1 (2022)
Publisher : Pusat Penelitian dan Pengembangan Bioteknologi dan Biodiversitas (P3BB) LPPM UNS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/jbb.v2i1.61198

Abstract

The phalaenopsis hybrid has become an economically important flower in the horticultural sector. Phalaenopsis Fuller's Pink Stripe Orchid is a hybrid orchid resulting from a cross between Phalaenopsis Fuller's Pink Swallow and Phalaenopsis Fullers Black Stripe. Rice washing water (leri) is a residue that accumulates when washing rice. This residue is usually thrown away when it is actually rich in various organic and mineral compounds. Waste that is wasted in vain can be used as nutrients for the growth of orchid plants. This research was conducted to study the effect of dose and frequency of leri water spraying on the growth of Phalaenopsis Fuller's Pink Stripe orchid at the vegetative stage. This study consisted of 10 treatments, namely giving 5 ml of water every 2 days (L1), 10 ml every 2 days (L2), 15 ml every 2 days (L3), 5 ml every 4 days (L4). a dose of 10 ml every 4 days (L5), a dose of 15 ml every 4 days (L6), a dose of 5 ml every 6 days (L7), a dose of 10 ml every 6 days (L8), a dose of 15 ml every 6 days (L9), and control treatment (L0). Each treatment was repeated 3 times. Based on the results of the study, it are often all over that there's no important interaction between the dose and also the frequency of administration of Leri water on the growth and performance of the Phalaenopsis Fuller's Pink Stripe.Keywords: Rice Wash Water, Phalaenopsis Fuller's Pink Stripe, vegetative growth
Yield of the Mutant (M6) Short Stem of Mentik Wangi Rice Varieties Resulting from Gamma Ray Irradiation 300 Gray Ahmad Yunus; Rizky Mulyawati; Parjanto Parjanto
Journal of Biodiversity and Biotechnology Vol 1, No 2 (2021)
Publisher : Pusat Penelitian dan Pengembangan Bioteknologi dan Biodiversitas (P3BB) LPPM UNS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/jbb.v1i2.56437

Abstract

Mentik Wangi is one of Indonesia's most popular rice germplasm sources due to its delicate, fluffy texture and arom aroma. Farmers switch to better varieties with high yields due to field challenges in cultivating fragrant rice. The plants are quite tall, harvest time is long, and yield is low; therefore farmers switch to superior varieties with high yields. The purpose of this research was to obtain information on how much yield of 14 mutant rice lines produce and to find mutant lines with short stems and high yields that could be developed to new varieties. The research was conducted at the Tegalgondo Rice Seed Garden Agricultural Land between June to October 2020. This study used a Completely Randomized Block Design (CRBD) with a single factor. Each treatment was repeated three times. The data obtained analyzed with analysis of variance and followed with Duncan Multiple Range Test at 5% level if any significant influences were obtained. The results of this research showed that 14 Mentik Wangi rice lines yields were ranging from 6.70 to  8.21 tons ha-1.Stems from 9 lines with high yields were M6-MW3-G10-14-2, M6-MW3- G12-1-17,  M6-MW3-G5-21-3,  M6-MW3-G5-21-14,  M6-MW3-G6-17-14-4,  M5-MW3-G6-20-9-16,  M6-MW3-G1-1-2  M5-MW3-G1-5-47,  M6-MW3-G6-10-9-26.  These lines were potential candidates for new high-yielding varieties, which would then considered to be evaluated for higher yields or multilocation tests before becoming an official new varieties.Keywords: mentik wangi; mutations; short stem; yield potential;
Isolation And Identification of Arbuscula Mycorrhiza (Ma) In Plant Rhizosphere in Greenbelt Area of PT. Semen Indonesia Tbk Tuban Avivi Nur Aina; Supiana Dian Nurtjahyani; Sriwulan Sriwulan; Eko Purnomo
Journal of Biodiversity and Biotechnology Vol 3, No 1 (2023)
Publisher : Pusat Penelitian dan Pengembangan Bioteknologi dan Biodiversitas (P3BB) LPPM UNS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/jbb.v3i1.72038

Abstract

The Green Belt area is a buffer area in an industrial area that functions as a barrier or separates the mining area from other regions, such as residential areas or community agriculture. The Green belt area consists of various plant vegetation that absorbs carbon dioxide and air conditioning, preventing sedimentation and erosion in the mining area. Arbuscular mycorrhiza has the potential to live in the green belt area by forming a mutualism symbiosis with plants or vegetation in the area. This study aimed to determine arbuscular mycorrhizae found in the Greenbelt area of PT Semen Indonesia Tbk Tuban. Sampling used purposive sampling techniques at three sampling stations, namely rhizosphere eucalyptus (Malaleuca cajuputi), trembesi (Samanea saman), and sawo (Manilkara zapota). The study was conducted in November 2021 through three stages: sampling, sample isolation, and species identification. The results showed seven types of arbuscular mycorrhiza from five different genera, namely the genus Glomus, Acaulospora, Gigaspora, Racocetra, and Archaeospora. The most found species is the genus Glomus. This research contributes as a source of literacy and information about the types of AMF found in PT Semen Indonesia's Greenbelt area, which can be used as biological fertilizer, support plant growth, help reclaim mining areas, and protect plants from drought pressure in mining areas.
Genetic Variability of Eggplant (Solanum Melongena L. 'Nasubi') Based on ISSR Markers and Phenotypic Characters Nabila Shafura; Annas Rabbani; Budi Setiadi Daryono
Journal of Biodiversity and Biotechnology Vol 2, No 2 (2022)
Publisher : Pusat Penelitian dan Pengembangan Bioteknologi dan Biodiversitas (P3BB) LPPM UNS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/jbb.v2i2.65874

Abstract

‘Nasubi’ eggplant seeds have occurred since 2018. In addition, plants that produce fruit phenotypic characters differ from those planted before the planting period in 2018. These three factors cause the decline in harvest rates and the current high increase in production costs. Observation of variations in phenotypic and molecular characters with ISSR molecular markers between seeds before 2018 and after 2018, became the first step for the research team to reveal information on the uniformity of phenotypic and molecular characters in eggplant ‘Nasubi’ from seeds purchased by farmers before 2018 and post-2018. The results of the molecular analysis with ISSR primary molecular markers namely UBC 809, UBC 815, IBC 880, UBC 888, and UBC 892, showed that the six samples had a high similarity index of 90% and resulted in a low polymorphism average of 12%. The results of the phenotypic analysis showed that eggplants from the seeds before 2018 and seeds after 2018 in this study had variations in the character of the leaf tip angle, leaf base, flower crown color, stamen color, young fruit color, fruit curve, and fruit tip shape.
Effects of Gamma Irradiation on Phenotypic Changes in Vanda Hybrid Sri Hartati; Prasetyo Prasetyo; Endang Setia Muliawati
Journal of Biodiversity and Biotechnology Vol 1, No 1 (2021)
Publisher : Pusat Penelitian dan Pengembangan Bioteknologi dan Biodiversitas (P3BB) LPPM UNS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/jbb.v1i1.49298

Abstract

Vanda Orchid is one of the most popular ornamental plants. Orchids have slow growth. One way to overcome this weakness is by using gamma-ray irradiation. This research aimed to study the morphological characters of various radiation doses resulting from the crossing of the Vanda Orchid. This study was carried out by observing the characters of vegetative growth for each plant on 0 Gy, 10 Gy, 20 Gy, 30 Gy, 40 Gy, and 50 Gy doses of gamma irradiation treatments. The observed variables were the increase in plant height, leaf length, root length, number of roots, number of leaves, leaf width, and leaf color. The results showed that the dose of gamma-ray irradiation were able to increase plant height. 10 Gy and 20 Gy, gamma-ray irradiation doses, resulted in discolored yellowing of orchid leaves. The discoloration of orchid leaves to light green occurs at irradiation doses of 30 Gy, 40 Gy, and 50 Gy. The morphological characteristics of Vanda sp. hybrid results gamma irradiation are high at dose 10-40 Gy. Keywords: characteristics; morphological; orchid.
EFFICIENCY OF PRODUCTION FACTORS USED IN CARROT (Daucus carota) FARMING Suswadi Suswadi; Agung Prasetyo; Dewi Gesang Nurlarasati
Journal of Biodiversity and Biotechnology Vol 2, No 1 (2022)
Publisher : Pusat Penelitian dan Pengembangan Bioteknologi dan Biodiversitas (P3BB) LPPM UNS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/jbb.v2i1.61785

Abstract

The farmers' income is uncertain in Boyolali following the unstable weather. This study aims to know the factors that affect the production of carrots and the efficiency level of production factors in carrot farming. The method used in this research was the survey method that was performed in the Argo Ayuningtani Farmer Group, Senden Village, Selo District, Boyolali Regency. The respondent was determined by observation of as many as 30 carrot farmers. The collected data were in the form of primary and secondary data. The primary data were obtained from literature studies and studies of other institutions that related to this research. Data were analyzed using quantitative analysis using the Cobb Douglass model. The results of the study found that several factors that simultaneously affected the productivity of carrot farming were the total area of land (X1), labor (X2), seeds (X3), organic fertilizer (X4), fertilizer Za (X5) used by farmers. The number of seeds is the most influential production factor in the productivity of the Argo Ayuningtani Farmer Group, Senden Village, Selo District, Boyolali Regency.

Page 2 of 4 | Total Record : 31

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