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Journal of Biodiversity and Biotechnology
Published by Universitas Sebelas Maret
ISSN : 27755223     EISSN : 28083229     DOI : https://doi.org/10.20961/jbb
Core Subject : Agriculture,
Agriculture, Biological Sciences & Forestry
Journal of Biodiversity and Biotechnology is a scientific journal focused on biodiversity and biotechnology published twice a year (June and December) managed and published by Universitas Sebelas Maret. This journal covers original research in the following areas: biodiversity and environment, biotechnology, agriculture biotechnology, food biotechnology, medical biotechnology, bioinformatics, molecular biology, biochemistry, bioinformatics, phyto-pharmacy, herbal medicine, and animal science. Other topics are welcome if it discusses about biodiversity or biotechnology.
Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Agronomi dan Ilmu Tanaman
Articles 60 Documents
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The Effect of Dosage and Frequency of Leri Water Spraying on Phalaenopsis Fuller's Pink Stripe Orchid Growth of Aclimatisation Sri Hartati; Ongko Cahyono
Journal of Biodiversity and Biotechnology Vol 2, No 1 (2022)
Publisher : Pusat Penelitian dan Pengembangan Bioteknologi dan Biodiversitas (P3BB) LPPM UNS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/jbb.v2i1.61198

Abstract

The phalaenopsis hybrid has become an economically important flower in the horticultural sector. Phalaenopsis Fuller's Pink Stripe Orchid is a hybrid orchid resulting from a cross between Phalaenopsis Fuller's Pink Swallow and Phalaenopsis Fullers Black Stripe. Rice washing water (leri) is a residue that accumulates when washing rice. This residue is usually thrown away when it is actually rich in various organic and mineral compounds. Waste that is wasted in vain can be used as nutrients for the growth of orchid plants. This research was conducted to study the effect of dose and frequency of leri water spraying on the growth of Phalaenopsis Fuller's Pink Stripe orchid at the vegetative stage. This study consisted of 10 treatments, namely giving 5 ml of water every 2 days (L1), 10 ml every 2 days (L2), 15 ml every 2 days (L3), 5 ml every 4 days (L4). a dose of 10 ml every 4 days (L5), a dose of 15 ml every 4 days (L6), a dose of 5 ml every 6 days (L7), a dose of 10 ml every 6 days (L8), a dose of 15 ml every 6 days (L9), and control treatment (L0). Each treatment was repeated 3 times. Based on the results of the study, it are often all over that there's no important interaction between the dose and also the frequency of administration of Leri water on the growth and performance of the Phalaenopsis Fuller's Pink Stripe.Keywords: Rice Wash Water, Phalaenopsis Fuller's Pink Stripe, vegetative growth
Yield of the Mutant (M6) Short Stem of Mentik Wangi Rice Varieties Resulting from Gamma Ray Irradiation 300 Gray Ahmad Yunus; Rizky Mulyawati; Parjanto Parjanto
Journal of Biodiversity and Biotechnology Vol 1, No 2 (2021)
Publisher : Pusat Penelitian dan Pengembangan Bioteknologi dan Biodiversitas (P3BB) LPPM UNS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/jbb.v1i2.56437

Abstract

Mentik Wangi is one of Indonesia's most popular rice germplasm sources due to its delicate, fluffy texture and arom aroma. Farmers switch to better varieties with high yields due to field challenges in cultivating fragrant rice. The plants are quite tall, harvest time is long, and yield is low; therefore farmers switch to superior varieties with high yields. The purpose of this research was to obtain information on how much yield of 14 mutant rice lines produce and to find mutant lines with short stems and high yields that could be developed to new varieties. The research was conducted at the Tegalgondo Rice Seed Garden Agricultural Land between June to October 2020. This study used a Completely Randomized Block Design (CRBD) with a single factor. Each treatment was repeated three times. The data obtained analyzed with analysis of variance and followed with Duncan Multiple Range Test at 5% level if any significant influences were obtained. The results of this research showed that 14 Mentik Wangi rice lines yields were ranging from 6.70 to  8.21 tons ha-1.Stems from 9 lines with high yields were M6-MW3-G10-14-2, M6-MW3- G12-1-17,  M6-MW3-G5-21-3,  M6-MW3-G5-21-14,  M6-MW3-G6-17-14-4,  M5-MW3-G6-20-9-16,  M6-MW3-G1-1-2  M5-MW3-G1-5-47,  M6-MW3-G6-10-9-26.  These lines were potential candidates for new high-yielding varieties, which would then considered to be evaluated for higher yields or multilocation tests before becoming an official new varieties.Keywords: mentik wangi; mutations; short stem; yield potential;
Isolation and Identification of Arbuscula Mycorrhiza (Ma) in Plant Rhizosphere in Greenbelt Area of PT. Semen Indonesia Tbk Tuban Avivi Nur Aina; Supiana Dian Nurtjahyani; Sriwulan Sriwulan; Eko Purnomo
Journal of Biodiversity and Biotechnology Vol 3, No 1 (2023)
Publisher : Pusat Penelitian dan Pengembangan Bioteknologi dan Biodiversitas (P3BB) LPPM UNS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/jbb.v3i1.72038

Abstract

The Green Belt area is a buffer area in an industrial area that functions as a barrier or separates the mining area from other regions, such as residential areas or community agriculture. The Green belt area consists of various plant vegetation that absorbs carbon dioxide and air conditioning, preventing sedimentation and erosion in the mining area. Arbuscular mycorrhiza has the potential to live in the green belt area by forming a mutualism symbiosis with plants or vegetation in the area. This study aimed to determine arbuscular mycorrhizae found in the Greenbelt area of PT Semen Indonesia Tbk Tuban. Sampling used purposive sampling techniques at three sampling stations, namely rhizosphere eucalyptus (Malaleuca cajuputi), trembesi (Samanea saman), and sawo (Manilkara zapota). The study was conducted in November 2021 through three stages: sampling, sample isolation, and species identification. The results showed seven types of arbuscular mycorrhiza from five different genera, namely the genus Glomus, Acaulospora, Gigaspora, Racocetra, and Archaeospora. The most found species is the genus Glomus. This research contributes as a source of literacy and information about the types of AMF found in PT Semen Indonesia's Greenbelt area, which can be used as biological fertilizer, support plant growth, help reclaim mining areas, and protect plants from drought pressure in mining areas.
Genetic Variability of Eggplant (Solanum Melongena L. 'Nasubi') Based on ISSR Markers and Phenotypic Characters Nabila Shafura; Annas Rabbani; Budi Setiadi Daryono
Journal of Biodiversity and Biotechnology Vol 2, No 2 (2022)
Publisher : Pusat Penelitian dan Pengembangan Bioteknologi dan Biodiversitas (P3BB) LPPM UNS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/jbb.v2i2.65874

Abstract

‘Nasubi’ eggplant seeds have occurred since 2018. In addition, plants that produce fruit phenotypic characters differ from those planted before the planting period in 2018. These three factors cause the decline in harvest rates and the current high increase in production costs. Observation of variations in phenotypic and molecular characters with ISSR molecular markers between seeds before 2018 and after 2018, became the first step for the research team to reveal information on the uniformity of phenotypic and molecular characters in eggplant ‘Nasubi’ from seeds purchased by farmers before 2018 and post-2018. The results of the molecular analysis with ISSR primary molecular markers namely UBC 809, UBC 815, IBC 880, UBC 888, and UBC 892, showed that the six samples had a high similarity index of 90% and resulted in a low polymorphism average of 12%. The results of the phenotypic analysis showed that eggplants from the seeds before 2018 and seeds after 2018 in this study had variations in the character of the leaf tip angle, leaf base, flower crown color, stamen color, young fruit color, fruit curve, and fruit tip shape.
Efficiency of Production Factors used in Carrot (Daucus carota) Farming Suswadi Suswadi; Agung Prasetyo; Dewi Gesang Nurlarasati
Journal of Biodiversity and Biotechnology Vol 2, No 1 (2022)
Publisher : Pusat Penelitian dan Pengembangan Bioteknologi dan Biodiversitas (P3BB) LPPM UNS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/jbb.v2i1.61785

Abstract

The farmers' income is uncertain in Boyolali following the unstable weather. This study aims to know the factors that affect the production of carrots and the efficiency level of production factors in carrot farming. The method used in this research was the survey method that was performed in the Argo Ayuningtani Farmer Group, Senden Village, Selo District, Boyolali Regency. The respondent was determined by observation of as many as 30 carrot farmers. The collected data were in the form of primary and secondary data. The primary data were obtained from literature studies and studies of other institutions that related to this research. Data were analyzed using quantitative analysis using the Cobb Douglass model. The results of the study found that several factors that simultaneously affected the productivity of carrot farming were the total area of land (X1), labor (X2), seeds (X3), organic fertilizer (X4), fertilizer Za (X5) used by farmers. The number of seeds is the most influential production factor in the productivity of the Argo Ayuningtani Farmer Group, Senden Village, Selo District, Boyolali Regency.
Analysis of Characteristics and Level of Efficiency on the Development of Organic Rice (Oryza sativa, L.) Farming Business in Boyolali Regency suswadi suswadi; Agung Prasetyo
Journal of Biodiversity and Biotechnology Vol 1, No 2 (2021)
Publisher : Pusat Penelitian dan Pengembangan Bioteknologi dan Biodiversitas (P3BB) LPPM UNS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/jbb.v1i2.56424

Abstract

Certified organic agriculture is a product that has competitiveness and opportunities for export. This study aims to determine and analyze the characteristics of farmers, the total cost and revenue and income of organic rice, ze the level of efficiency, and BEP (Break-Even Point) of certified organic rice farming. The sampling method used in this study was simple random sampling with a sample of 40 farmers calculated using the Slovin formula. The analysis used is: (1) the analysis of the mean score for the characteristics of the farmers, (2) the method of analyzing costs and revenues and farm income, and (3) the analysis of the efficiency of the farming business using the R/C (Return Cost Ratio) analysis method. The research location was determined purposively, namely Dlingo Village, Mojosongo District, Boyolali Regency. The results of the study concluded that: (1) The characteristics of organic farmers were that men are more involved than women, the age of organic farming business practicioners were above 50 years old, most of the farmers in Dlingo village have lived for more than 30 years, the main livelihood farmers in the agricultural sector. (2) Based on the total average cost for organic farming, the certified group has an average of Rp.20,495,000,- and The average revenue was Rp. 35,000,000,-. The income per planting season is Rp. 14.505.000,-. Organic certified farmer groups get an R/C ratio value of 1.7;  the R/C > 1 means that farming with certified organic systems is efficient. Rice farming with a certified organic system is profitable because the BEP value of production volume was 2,927 < farmers' production, while the BEP value for production price was 4,099 < selling price at the farmer level.Keywords: organic rice; income; efficiency;
Risk Factors for H9N2 Contamination in Live Bird Markets of the Greater Jakarta, Indonesia Rahma Hani Aisah; Bambang Sumiarto; Hendra Wibawa; Widagdo Sri Nugroho; Aprizal Panus; Sodirun Sodirun
Journal of Biodiversity and Biotechnology Vol 3, No 1 (2023)
Publisher : Pusat Penelitian dan Pengembangan Bioteknologi dan Biodiversitas (P3BB) LPPM UNS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/jbb.v3i1.74263

Abstract

Low Pathogenicity Avian Influenza virus subtype H9N2 causes decreased egg production and two of the four defined H9N2 poultry lineages (G1 and Y280) have been associated with human infections. The high positive test results for the H9N2 virus in the LBMs of the Greater Jakarta area in 2021 can be a source of transmission for the surrounding area. This study aims to determine the prevalence and risk factors that play a role in the H9N2 contamination in the LBMs to obtain a multivariate analysis model. A cross-sectional study was conducted on 87 vendors and 124 broiler chickens from 44 LBMs randomly selected in Tangerang City, North Jakarta, and Bekasi City. Samples were taken as equipment swabs at the stall of poultry carcass vendors, tracheal swabs, and cloacal swabs of broiler chicken. The test used was Real-Time Reverse Transcription Polymerase Chain Reaction. Data analysis was performed using univariate, bivariate, and multivariate analysis with logistic regression. The prevalence of H9N2 virus contamination at the LBM level in Greater Jakarta in 2022 was 77.27% (95% CI, α 7%), while the prevalence at the vendor level was 54.02% (95% CI, α 5%). The risk factors for H9N2 virus contamination with a significant p-value<0.05 include the positive results on equipment swab samples, positive results on both equipment and broiler swabs, and the use of carcass sink.  The multivariate analysis model of H9N2 virus contamination at the vendor level was Logit P (H9N2=1 | x) = 0.29924 - 2.42176PPE + 1.58691carcass sink. The risk factor contributing to increase H9N2 contamination is using carcass sinks, while PPE is the one that reduces H9N2 contamination.
Root Growth Response of Soybean Under Water Deficit Achmad Fatchul Aziez; Agung Prasetyo; Paiman Paiman
Journal of Biodiversity and Biotechnology Vol 2, No 2 (2022)
Publisher : Pusat Penelitian dan Pengembangan Bioteknologi dan Biodiversitas (P3BB) LPPM UNS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/jbb.v2i2.66465

Abstract

Roots are plant organs that function to absorb water and nutrients from the rhizosphere. If the soil is dry, the roots will be affected first. This study aims to know the response of soybean root to drought stress. This research was arranged in a randomized completely block design (RCBD) with two factors and three replications. The first factor was soil moisture content, which consisted of four levels, e.i., 100, 75, 50, and 25% field capacity. The second factor was the growth stage, which consisted of three kinds, e.i., the vegetative active, flowering time, and seed filling period. The results showed that the soil water content in below 75% field capacity decreased root length, root fresh weight, root dry weight, root volume, and increased shoot root ratio. The seed filling period was more sensitive to water deficiency than the active vegetative and flowering time. The study findings that soybean plants can grow well at 100% field capacity. The practical implication of planting soybeans use a soil moisture content of 100% field capacity.
Diversity of Sapodilla (Achras zapota L.) Growth from Wonogiri and Bojonegoro in Different Age of Transplantation Seed Endang Yuniastuti; Muhammad Agung; Parjanto Parjanto
Journal of Biodiversity and Biotechnology Vol 2, No 1 (2022)
Publisher : Pusat Penelitian dan Pengembangan Bioteknologi dan Biodiversitas (P3BB) LPPM UNS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/jbb.v2i1.61170

Abstract

Most farmers determine the cutting of air layer seed in a corp through the amount of the growing roots from air layer itself. The roots can be an indicator of air layer cutting method in a corp. The roots, which are too old or too young, grow less optimal in the air layer seed after being transferred into another cultivated medium. It is necessary to have knowledge about the precise age of air layer cutting so that the vegetative propagation by using air layer can be performed accurately and efficiently. The treatment that is executed is the age of air layer and the corps accession. The result of this research indicates that the effect of the difference air layer age on plant growth is at the age of emerging buds, aged 180 Day is treated with the age of the fastest emerging shoots. The growth of Bojonegoro air layer plants results that does not differ with Wonogiri air layer plants. There is no interaction between air layer age with sapodilla accession on plant growth.
Morphological Characters of Early Harvest Age Mutant Selection M3 Generation of Black Rice (Oryza sativa L.) Irradiated by Gamma Ray Nandariyah - Nandariyah; Parjanto Parjanto; Sutarno Sutarno; Riyatun Riyatun; Rifqi Syarif Muhammad
Journal of Biodiversity and Biotechnology Vol 1, No 2 (2021)
Publisher : Pusat Penelitian dan Pengembangan Bioteknologi dan Biodiversitas (P3BB) LPPM UNS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/jbb.v%vi%i.56382

Abstract

Black rice contains higher levels of anthocyanin, fiber, and protein compared to local white rice. The price of black rice is higher compared to local white rice, it is one of prove that black rice has functional and economical values. The main obstacles in the cultivation of black rice are the long harvest age, high plant height, and lack of productivity. One way that can be applied to overcome this dissadvantageis with the utilization of gamma ray irradiation. The purpose of this research is to get the early-season rice, short stem, and higher productivity. This research was conducted in Tempel, Gatak, Sukoharjo District, by planting M3 seed (gamma ray irradiation of 300 Gray dosage on M2). The results of black rice from gamma ray irradiation of 300 gray dosage are M-CI-03-239, M-CI-03-244, M-CI-03-246, M-CI-03-346, M- CI-03-350, M-CI-03-234, M-CI-03-346, M-CI-03-350, M-CI-03-250, M-CI- 03-346, M-CI-03-CI-03-262, M-CI-03-265, M-CI-03-270, M-CI-03-283, and M-CI-03-311 with the agronomical properties of early age, i.e 2 weeks faster (64 days) with control (80 days). There are 125 short-postured plants of gamma ray irradiation result with  99.2% percentage. There are 12 individual plants in the M3 strain (M-CI-03-240, M-CI-03-251, and M-CI-03-261) of gamma ray irradiation with the maturation category, short stems, and high productivity compared with control. The difference of productivity of irradiated crops with control is 57,91%.

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