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Journal of Global Pharma Technology
Published by Universitas Udayana
ISSN : 09758542     EISSN : -     DOI : -
Core Subject : Health,
Medicine & Pharmacology
ournal of Global Pharma Technology is a monthly, open access, Peer review journal of Pharmacy published by JGPT Journal publishes peer-reviewed original research papers, case reports and systematic reviews. The journal allows free access to its contents, which is likely to attract more readers and citations to articles published in JGPT. JGPT publishes original research work that contributes significantly to the scientific knowledge in pharmacy and pharmaceutical sciences- Pharmaceutics, Novel Drug Delivery, Pharmaceutical Technology, Cosmeticology, Biopharmaceutics and Pharmacokinetics, Pharmacognosy, Natural Product Research, Medicinal Chemistry, Computational Chemistry and Molecular Drug Design, Pharmaceutical Analysis, Pharmacology, Pharmacy Practice, Clinical and Hospital Pharmacy, Cell Biology, Genomics and Proteomics, Pharmacogenomics, Bioinformatics, Biotechnology and Applied Computer Technology. For this purpose we would like to ask you to contribute your excellent papers in pharmaceutical sciences.
Arjuna Subject : Kedokteran - Onkologi
Articles 2,438 Documents
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Study the Correlation between Chlamydial Infection and Some Novel Biomarkers in Fertile Women Basma Salman
Journal of Global Pharma Technology Volume 09 Issue 12
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Abstract

Objective: The aim of present study was designed to detect of chlamydia trachomatis bacteria in infertile women, and its correlation with some novel biomarkers includes (AMH and MFG-E8).Methods: The current study include 60 infertile subjects with age range 15-45 years and 30 healthy control subjects, divided into four groups depending on diagnosis with chlamydia trachoma is IgG. Results: The present study had been shown a high significant differences between four groups were [P ˂ 0. 001] according to chlamydia trachomatis Ig-G infection and MFG-E8 marker. There are highly significant differences among percentage of AMH levels (high, low& normal), in fertile and infertile subjects. The present study showed no significant statistical difference were [p>0.05] among the effect of AMH on age, FSH, LH, PRL, Testo, and MFG-E8 in infertile patients. According to the results of present study that has been shown a highly significant difference [p ˂ 0.006 and 0.003, respectively] in FSH and prolactin levels between fertile and infertile patients, while the serum levels of LH, Testosterone and MFG-E8 show no significant differences [p˃0.05] in both fertile and infertile patients. Conclusion: The results of the present study showed a significant correlation between chlamydial infection andMFG-E8 marker while no significant correlation between chlamydial infection and AMH marker.Keywords: female infertility, chlamydia trachomatis, AMH, MFG-E8.
The Effect of the Method of Practical Presentations to Develop Mental Toughness and Learn Some of Basic Skills for Students in Volleyball Ayad Nasser Hussein
Journal of Global Pharma Technology .
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Abstract

The successful teaching helps the learner and the teacher to achieve the planned goals, and depends largely on the good selection of teaching methods that regulate student learning and facilitate them to reach a high degree performing goals of perfection, the teaching methods play an important and fundamental role in the educational situation, and its mastery saves the economy in the time and effort spent, not only that role on the information only, but includes skills, tendencies, trends, thinking methods and others. Through the follow-up of field researchers to teach volleyball in the lesson of physical education, he noted that many of the learners have difficulty in learning basic skills as a result of their teaching methods, and that their teaching needs other methods that lead to the best possible learning in accordance with the development of the game And as appropriate to stimulate the learners' motivation towards learning, and thus reflected on the upgrading of this game and the objective of research to:The preparation of a program in a way practical presentations in the development of mental toughness and learn the basic skills for the students in volleyball.To know the impact of the educational program presentations practical way to develop mental toughness and learn the basic skills for the students in volleyball.The researchers used the experimental program and the research community of the second stage students in the Faculty of Physical Education and Sports Sciences at the University of Babylon for the academic year 2016-2017 (86) students representing the people (B - C - D). The sample of the research was chosen by the random way and represented (24) students after the exclusion of the students and the players of the game and divided into two groups control and experimental, the conclusions were:· There is the impact of the educational program presentations practical way to develop mental toughness basic skills for the students in volleyball.· There is the evolution of the control group in the development of mental toughness basic skills for the students in volleyball.· There is an advantage in the experimental group to develop mental toughness basic skills for the students in volleyball.· The method of practical presentations took into account the individual differences between the students, so the evolution was evident in all the variables.Keywords: Practical presentations, Mental toughness and basic skills.
Performance Training for the Development of the Harmonic Ability and its Impact on the Rapid Force and the Achievement of (50) Meters of Breast for Junior Wadad Kadhim Majid
Journal of Global Pharma Technology Volume 11 Issue 6.
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Abstract

In the introduction and importance of the research, the importance of the muscular ability to harmonize the formation and formulation of specific physical characteristics of many games with motor performance, including swimming, which need to link the strength and speed together to the muscles working in the performance, and the importance of research in the use of training exercises to perform muscle training Harmonic characteristic of the speed of the muscles of the arms and legs to promote the players, which in turn is working on the development of the completion of (50) meters breast. The problem of research is that many players are characterized by good physical and physical ability, but cannot perform any movements that require a certain amount of coherence and compatibility at the same time, the chest swell is far from the normal movements performed by the player in his public life, as well as it needs to High level of motor timing and muscle compatibility, which is the blows of the legs and the blows of the arms and the process of linking them to the breathing process, which are not familiar to the swimmer, especially in the younger ages of them. The aim of the study is to prepare a performance training to develop the musculoskeletal capacity of the two legs and arms of the young swimmers. 2 - Identify the effect of special exercises in the development of the rapid strength of the muscles of the legs and arms and the completion of the 50-meter breaststroke ages (11-13) years. The research hypothesis is that there are statistically significant differences in the effect of adaptive ability exercises in the results of the pretests on the dimension in the development of the rapid strength of the muscles of the legs and arms and the completion of (50) m. The researchers identified the research society and its sample with the specialized school for boys (11-13 years old) and officially registered in the Central Swimming Federation who continue in training and competition in the sports season (2017-2018) of (8) swimmers. Their number (4) players. Four of them were excluded due to repeated absence, thus representing 50% of the research community. The special exercises were carried out within the training curriculum for the sample which lasted eight weeks and three days a week for 24 training units. The researchers found several conclusions that the special compatibility ability exercises led to the development of muscular strength of the arms and legs in the junior swimming pool. Special compatibility resulted in the development of muscular capacity for both arms and legs, which had a good effect in the development of the digital achievement of (50) meters in the sample junior swim.Keywords: Performance training, harmonic ability and rapid force.
Estimation and Comparison of the Levels of Elastase in Saliva, GCF & Serum of Smokers and Non-smokers with Chronic Periodontitis -A Clinico-biochemical Study S. Srinivasan
Journal of Global Pharma Technology Volume 12 Issue 10 (2020) Oct. 2020
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Objective: This study was done to estimate and compare the levels of Elastase in Saliva, GCF & Serum of smokers and non-smokers with chronic periodontitis. Materials and Method:125 male subjects in the age group of 21-50 years were enrolled and categorized as Group 1, Non-smoking subjects with healthy periodontium Group 2, Smoking subjects with healthy periodontium Group 3, Non-smoking subjects with Chronic periodontitis Group 4, Smoking subjects (10 Cigarettes/day) with Chronic periodontitis. Plaque Index, Gingival Bleeding Index, Probing Pocket Depth, Clinical Attachment Level, Salivary, GCF and Serum Elastase levels were recorded and evaluated using sandwich ELISA method. Results: The clinical parameters expressed a significant difference between diseased group and healthy group. Elastase levels (Saliva, GCF and Serum) showed significant difference between diseased group and healthy group. In between the diseased group comparison, GCF and Saliva showed Lower Elastase levels in smoking periodontitis group. Whereas, Serum showed higher Elastase levels. Conclusion: Elastase can be used as a reliable biomarker for smokers and non-smokers with chronic periodontitis to denote disease activity. Keywords: Periodontitis; Smoking; Elastase; Saliva; Gingival crevicular fluid.
Investigation of Antioxidant Status in Iraqi Patients with Beta- Thalassemia Major Jamela Jouda
Journal of Global Pharma Technology Vol. 9 Issue 7 : 2017
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Abstract

Background: Oxidative stress is the most common complication in Beta-thalassemia patients due to ironover load that is resulted from long life blood transfusion leading to growth retardation, delay of sexualmaturation, and later involvement of the liver, heart, and endocrine system. Aim of study: Assess of someantioxidant parameter status in serum and saliva of patients with beta-thalassemia major in comparisonwith control subjects. Methodology: Total antioxidant (TAO), glutathione (GSH), uric acid (UA), urea (U)and albumin were investigated in serum and saliva samples of all subjects. Results: Albumin level inserum is significantly (P ˂ 0.00001) decreased in patients group (3.27 ± 0.17 g/dl) from that in controlgroup (3.59 ± 0.13 g/dl). However, TAO level was significantly elevated (P = 0.031) from (3.14 ± 1.38U/ml) in the saliva of control group up to (4.09 ± 1.76 U/ml) in the saliva of patients group as well assignificant difference (P = 0.02) in salivary fraction index (Sf) only for TAO which is elevated from 0.47 incontrol group up to 0.56 in patients group. Conclusion: The findings of this study indicated that theserum level of albumin can be used as a good marker to reflect the liver status in TM patient in additionto other conventional markers. Also this study suggested that saliva can be act as an alternative sampleto assess the antioxidant status in TM patients but need further investigation.Keywords: Beta thalassemia major, Iron overload, Antioxidant, Saliva.
Isolation and Diagnosis of Staphylococcus spp. using VITEK-2 System from Nasal and Ear of Healthy Carriers Sddiq Ghani Al-Muhanna
Journal of Global Pharma Technology Volume 11 Issue 09: (2019) September 2019
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One hundred of nasal and ear swabs were collected from healthy students of Babylon University who not suffering from any upper respiratory tract infection and have not received any antibiotics from the period of two. All they were cultured on suitable media .The positivity of culturing on Mannitol salt agar (selective media for Staphylococci) was 20% distributed as 14% for nasal swabs and 6% for ear swabs. When Biochemical and Serological tests were used for identifying Staphylococcus species, the results showed that 45% of these isolates were Coagulase positive while 55% were Coagulase negative .Staphylococcus species were identified by using VITEK-2: Staphylococcus aureus 45%, S. epidermidis 20%, S.capitis sub.capitis20% and S.cohni. Sub. cohni 10% .Antibiotic sensitivity test for Staphylococcus isolates showed sensitivity for ciprofloxacin to all isolates while appeared resistant to Nalidixic acid, Kanamycin  and Gentamycine, most of Staphylococcus aureus isolates were resisted to Methicillin antibiotic. 
REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION-A REVIEW Sri, K. Bhavya; Banu, Shaheen; Sumakanth, Mogili
Journal of Global Pharma Technology Volume 16 Issue 03 (2024) March 2024
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The reverse transcription polymerase chain response is straightforward, powerful, and exquisite innovation in the discipline of molecular biology. It helps in determining the gene expression and amplifying the gene of interest in numerous fields used to locate and quantify the quantity of a given DNA collection. It entails three steps: reverse transcription, PCR amplification, and electrophoresis. Among PCR, the maximum commonly used PCR technique is reverse transcription polymerase chain reaction abbreviated as RT-PCR. it is way utilized in molecular biology & genetic research that allows the detection & quantification of mRNA. in this, RNA template is first converted into a complementary DNA by use of a reverse transcriptase enzyme and is further used for exponential amplification the use of the PCR method. Many genes are recognized to have their own promoter regions that inform cells where to begin transcribing the unique gene. RT-PCR first starts by using the usage of reverse transcriptase enzyme to make DNA out of RNA that has been extracted from cells or tissue samples. The RNA is in the shape of a polymer. The opposite transcriptase copies the RNA into an open-ended DNA strand, preventing it when it encounters a unique non-coding location known as a "poly-A tail" for each 20 to 23 nucleotides. The PCR method then begins by blending the newly made DNA with known primers to expand or reflect a selected piece of DNA. The pattern is then heated and cooled more than one instance, which causes the DNA to split into exclusive string sizes, brief and long. the quick string represents the newly copied RNA that can be visible as amplification, while the longer strand represents each the amplified RNA and all other non-amplified DNA portions collectively. The difference in the duration of DNA strands is what offers reverse transcriptase PCR its name. RT-PCR approach is performed in a thermocycler for the huge production of genetic fabric. It includes several wells, thermos regulators, a detector, and a gadget hooked up with appropriate software. Keywords: Amplification, Thermocycler, Gene expression, cDNA, Molecular biology, RT-PCR, Template.
EXPLORING AMYLASE ACTIVITY IN BACTERIA ISOLATED FROM CEREAL - INFESTING INSECTS: A MICROBIOLOGICAL APPROACH Thakare, Dattabhushan Karvir; Hamde, V.S.; Narsinge, A.P.; Kadam, Aditya S.; Dixit, Ankita M.
Journal of Global Pharma Technology Volume 16 Issue 07 (2024) July 2024
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Amylases are significant industrial enzymes with diverse applications across various industries. This study aimed to isolate and screening of amylase-producing microorganisms from the gut and salivary gland microflora of cereal-spoiling insects. Samples were collected from cereals infested by pests such as the lesser grain borer, Granary weevil, Rice weevil, and Angoumois grain moth in the Beed district. The digestive tracts of the third and fifth instar larvae of Sitophilus granarius were dissected, homogenized, and serially diluted. The dilutions were spread on nutrient and starch agar plates and incubated to isolate gut flora. Bacterial isolates were tested for amylolytic activity using the starch hydrolysis test. Among the isolates, one coded as SG6 demonstrated the highest amylolytic activity with a 3 mm zone of clearance on starch agar plates. Morphological and Gram staining analysis identified the isolate as gram-positive and rod-shaped. The clear zones observed around the colony indicate starch hydrolysis by amylase production. The colony characteristics of isolate SG6 were circular, medium-sized, and white, with smooth edges, raised elevation, no pigmentation, and motile nature. This study confirms the presence of amylase-producing bacteria in the gut microflora of cereal-spoiling insects and highlights the potential of isolating SG6 for industrial applications. Keywords: Amylase production, Cereal-spoiling insects, Gut microflora, Starch hydrolysis, Sitophilus granaries.
ROLE OF NANOTECHNOLOGY IN CURRENT ADVANCEMENT VACCINE DEVELOPMENT FOR COVID-19 Mohanty, R
Journal of Global Pharma Technology Volume 16 Issue 01 (2024) January 2024
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The most recent human coronavirus known as COVID-19(SARS-CoV-2) has already spread around the world. This review describes the accumulated knowledge of the previous SARS-CoV, MERS-CoV infections and aim to help understand the newly emerged SARS-CoV-2 infection. Advances in nanotechnology over the past decades to develop new nanomaterials and step forward in the application of new technology tools. Finally nanotechnology approach describes vaccine development and therapeutics drugs developed nanomedicines which are currently undergoing clinical trial to become innovative alternatives for overcoming COVID-19 vaccine. Nanoparticles are non-toxic antiviral showed great promise to provide nano vaccine against viral infection. Keywords: Nanotechnology, Vaccine Development, COVID-19.
AGE-STRATIFIED PREVALENCE AND ANTIBIOTIC RESISTANCE PATTERNS OF HELICOBACTER PYLORI INFECTION AMONG PATIENTS UNDERGOING UPPER GASTROINTESTINAL ENDOSCOPY IN A TERTIARY CARE HOSPITAL, INDIA Ahmad, Imtiyaz; Hassan, Syed Belal; Mustaqueem, Syed Fiza; Ahmad, Ausaf
Journal of Global Pharma Technology Volume 16 Issue 04 (2024) April 2024
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The infection rate of H. pylori varies significantly between developing and developed nations, with rates approximately four to five times higher in developing countries. Antibiotic misuse has contributed to resistance issues, complicating eradication efforts. Study aimed to assess age-specific H. pylori prevalence and antibiotic resistance among patients undergoing Upper Gastrointestinal Endoscopy at a tertiary care hospital in India. Study included patients aged 14 to 86 presenting with symptoms like dyspepsia and epigastric pain. Demographic data and treatment history were collected, excluding recent antibiotic or proton pump inhibitor users. Statistical analysis was performed using SPSS version 16.0. Results indicated an overall prevalence of 61.1%, with similar rates across genders. Infection rates peaked at 73.7% in patients above 70 years. Positive endoscopic findings correlated significantly with H. pylori infection, underlining the importance of endoscopic evaluations. Notably, symptoms like epigastric pain were linked to H. pylori positivity. The study concluded that H. pylori prevalence is notably high in individuals above 70 years old. Additionally, it shed light on gender-specific prevalence patterns and highlighted metronidazole resistance as the most common, followed by levofloxacin and amoxicillin resistance. Furthermore, the study stressed the importance of endoscopic examinations in diagnosing H. pylori infection. Keywords: Antibiotic Resistance, Dyspepsia, Epigastric pain, H. pylori, Prevalence.

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