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Journal of Global Pharma Technology
Published by Universitas Udayana
ISSN : 09758542     EISSN : -     DOI : -
Core Subject : Health,
Medicine & Pharmacology
ournal of Global Pharma Technology is a monthly, open access, Peer review journal of Pharmacy published by JGPT Journal publishes peer-reviewed original research papers, case reports and systematic reviews. The journal allows free access to its contents, which is likely to attract more readers and citations to articles published in JGPT. JGPT publishes original research work that contributes significantly to the scientific knowledge in pharmacy and pharmaceutical sciences- Pharmaceutics, Novel Drug Delivery, Pharmaceutical Technology, Cosmeticology, Biopharmaceutics and Pharmacokinetics, Pharmacognosy, Natural Product Research, Medicinal Chemistry, Computational Chemistry and Molecular Drug Design, Pharmaceutical Analysis, Pharmacology, Pharmacy Practice, Clinical and Hospital Pharmacy, Cell Biology, Genomics and Proteomics, Pharmacogenomics, Bioinformatics, Biotechnology and Applied Computer Technology. For this purpose we would like to ask you to contribute your excellent papers in pharmaceutical sciences.
Arjuna Subject : Kedokteran - Onkologi
Articles 2,439 Documents
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Evaluation of Nucleated Red Blood Cell Morphology Flag Screening with ADVIA® 2120 R. Mustarichie
Journal of Global Pharma Technology Volume 12 Issue 06 (2020) June 2020
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Abstract

The evaluation of nucleated red blood cell morphology flag screening with ADVIA® 2120 on behalf of blood smear has been investigated. The accurate and timely reporting of nucleated red blood cells (NRBC) is an important function of the clinical hematology laboratory because the presence of NRBC in human peripheral blood can show a special clinical status. It has been used 40 samples to evaluate an NRBC screening for the ADVIA® 2120 Hematology System with peripheral blood smears. The positive samples have a criteria % NRBC in range 0 – 15%. (in 100 leucocytes). The sensitivity and specificity for the presence of NRBC for all samples analyzed was 90 % and 63,33 %, respectively. There is a correlation on NRBC result screening between ADVIA® 2120 and peripheral blood smears with α 0,003 of spearman correlation. Keywords: Nucleated red blood cell (NRBC, flag, Clinical test, ADVIA® 2120.
The Impact of Training Pattern According to the Function Exercises of Play to Develop the Some of Physical Capabilities and the Performance Accuracy of the Fundamental Skills in Soccer Adnan Fadous Omar
Journal of Global Pharma Technology .
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Abstract

The research problem indicates a weakness and a decrease in the level of some special physical abilities as well as a decrease in the accuracy of the performance of some basic skills of the research sample and this is clearly evidenced by the slow movement and frequent maneuvers or cases and the failure of most cases scoring. The main objective of the research was to develop training pattern according to the training exercises in the training curriculum and to identify the effect of these training pattern on the research variables under consideration. The research hypotheses were statistically significant differences in the results of pre and posttests between the control and experimental research groups, statistical significance between the posttests between the control and experimental research groups and for the benefit of the experimental group. The researchers were used the experimental method, the sample of research consisted of (20) players representing the club (Ararat) the experimental group and (20) players representing the Club (Shaklawa) total control, the most important conclusions reached by researchers that the training models according to the exercise exercises used in The research has developed in some special physical abilities and accuracy of the performance of the basic skills used in the research. The recommendations are the adoption of training models according to the training exercises in the preparation stage and the two repetitive training methods in the training curricula of trainers.Keywords: Training pattern, Exercises, Physical capabilities, Fundamental skills and soccer.
Person’s Health Creation Potential as a Resource of Integrated Health Anatoly G Madzhuga
Journal of Global Pharma Technology Volume 08 Issue 08
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Abstract

Introduction: The paper deals with the genesis of the notion of “potential”, offers a vector-contextual model of person’s health creation potential, and reveals psychological essence of the specified phenomenon. Basing on the results of empirical study, the authors prove that person’s health creation potential is a multidimensional structure integrating three factors – valeological mindset, metacognitive abilities, and affective self-attribution. For the first time, the paper represents the characteristic of resource provision of human health in the context of a holistic approach. Besides, it shows and scientifically justifies the structure of health creation potential, which acts as a mechanism of human self-cognition, self-evaluation, and self-development.Method: The authors use a method of vector modelling, which enables to create a vector-contextual model of person’s health creation potential. This model allows us to define milestones for elaborating and approving special impacts from pedagogues, parent, and administration, which provide the formation of the culture of learners’ health and their personal position concerning health creation.Results:A vector-contextual model of person’s health creation elaborated and conceptualized by the authors enables a pedagogue to forecast sustainable qualities of learners’ self-realizing creative personality and levels of its readiness to health creation activities in constantly changing conditions of society and environment. It plays a significant role in the development of human intelligence and creation and has an impact on the protection, formation, and strengthening of human health.Discussion or Conclusion: Basing on theoretical analysis of literature detoted to the topic of study, the authors represent a refined definition of “health creation potential” notion, design its vector-contextual model, and justify main components-vectors: valeological mindset, metacognitive abilities, and affective self-attribution in the structure of person’s health creation potential. In the context of the topic under study, the authors define conceptual-theoretical models of health-creation potential, which create perspectives for applied research in the methodology of theory of individual health and optimization of the process of actualization of health creation potential at various periods of ontogenesis. Keywords: Health, Vector-contextual model, Valeological mindset, Metacognitive abilities, Affective self-attribution, Health creation competence.
Effect of Anticoagulant on Acetylsalicylic Acid and Salicylic Acid Analysis in Plasma In Vitro with High Performance Liquid Chromatography Yahdiana Harahap
Journal of Global Pharma Technology Volume 11 Issue 01.
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Abstract

Background: Evaluation of the anticoagulant effect and analysis of acetylsalicylic acid (ASA) and salicylic acid (SA) is important. Full validation of in vitro methods using citrate anticoagulant in the form of CPD-A has previously been done, whereas in the implementation of in vivo studies, EDTA and heparin were used as anticoagulants. Based on EMEA 2011, if there are anticoagulant changes in validated analysis methods then partial validation should be performed. Objective: This study aims to evaluate the effect of different types of anticoagulants on the analysis of ASA and SA in plasma after being preceded by partial validation. Materials and Method: Analysis was performed using high pressure liquid chromatography column C18 (Waters, ReliantTM 5μm; 250 x 4.6 mm); mobile phase acetonitrile - phosphate buffer 20 mM (35:65) with pH 2.5; 1.0 mL/min flow rate; column temperature 35˚C; 14 minutes of time analysis with furosemide as internal standard. Results: Accuracy and precision in plasma citrate, heparin, and EDTA analysis fulfilled linear calibration curves in ASA and SA. Stability and recovery of ASA and SA in plasma with different anticoagulants showed no significant difference, however the peak area response ratio for the three types of anticoagulants between plasma with anticoagulant heparin-citrate and EDTA-citrate showed significant difference and plasma with heparin anticoagulant provides a larger area than plasma with EDTA anticoagulants. Conclusion: From this experiment, the analytical methods obtained were considered to meet the validation requirements for use of citrate, heparin, or EDTA anticoagulants based on EMEA 2011.
Molecular Detection of hlyA Gene from Escherichia coli hemolytic isolated from Intestinal and Urinary Tract infections Raid Razzaq Ojaimi
Journal of Global Pharma Technology Volume 11 Issue 07
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Abstract

An Intestinal and Urinary tract infection is a common clinical problem worldwide. In this study 76 isolates Escherichia coli were isolated from midstream urine specimens only 30 isolates show ability to hemolysis, and 80 isolated Escherichia coli from diarrhea specimens only 10 isolates show ability to hemolysis. In this study, hlyA gene responsible for hemolysin production were detected, from fifteen clinical isolate distributed on 10 isolates of urine and 5 isolates of diarrhea of E.coli collected from 40 isolates for hemolysin produced by using PCR techniques. hlyA gene was present in all isolates. Keywords: Hlya, Escherichia coli, Hemolysin.
Effect of Two Species of Ticks and Lice on Some Blood and Biochemical Parameters and their Role in the Transmission of Toxoplasma Gondii to Turkey Meleagris Gallopavo during Spring and Summer Seasons in AL-Diwaniyah Province – Iraq Khalid Thamir Mattar AL-Shaibani
Journal of Global Pharma Technology Volume 10 Issue 06: (2018) June 2018
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Abstract

AL-Diwaniyah governorate was the research place, where the study was conducted in during spring and summer seasons from the start of March 2017 to the end of October 2018. 120 turkey birds were selected from some districts and areas of AL-Diwaniyah governorate such as AL-Diwaniyah, AL-Shamiya, AL-Daghara,AL- Sanniyah, AL-Hamza and Afak, With 20 birds per region (10 per season). Two species of ticks: Argus persicus and Dermacentor variabilis were diagnosed with 15% and 18.3% respectively, and two species of lice: Goniocotes gallinae and Menacanthus straminus with 37.5% and 27.5% sequentially. The results showed a significant increase in the Infection of ticks and lice in spring compared to summer, where the rate of infection of tick and lice was 51.66% and 85%, respectively, while infection rate in summer was 23.33% and 35%, respectively. Infection by lice was high during spring in the areas of AL-Dagara, AL- Saniyah,and AL- Hamza . It was observed that there increasing in the numbers of isolated lice species in these areas in comparison with the number of tick, whereas the lowest rates of infection by ticks and lice were in the province center.  The results revealed a significant decreasing ( P ˂ 0.05 )in the concentration of Hb, the volume of PCV and the total number of RBC of infected birds compared with non-infected, while a high significant increasing was observed in the total number of WBC of infected birds in comparison with non-infected ones. The results of biochemical parameters of serum showed a significant decrease P (0.05) in the values of total protein concentrations, cholesterol and glucose of the infected birds' serum. The lowest values were for infected birds with lice, followed by tick compared to control. Eye examination of the skin and feathers of infected birds illustrated the presence of important changes such as the fall; fracture and lack of feathers completely in some areas of skin as well as wounds, scratches, and bleeding were clearly observed.  The results of polymerase chain reaction (PCR) of tick A. persicus and lice M. stramineus tissues showed that diagnostic gene B1 (399bp) of T.gondii was present in 20 samples of the total number. This indicates the presence of this parasite in its tissues and ability to transmit it.Keywords: Turkey, Tick, Lice, PCR,
Novel Mutations Related with Von Willebrand Disease in Iraqi Patients Ali H. AL-Musawi
Journal of Global Pharma Technology Volume 9 Issue 10
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Abstract

Von Willebrand Disease (VWD) is the most common hereditary bleeding disorder in humans, with an estimated prevalence ranging upward to 1% of the general population. Males and females are both approximately equally affected. VWD arises from a deficiency or dysfunction of von Willebrand factor (VWF). The aim of this study was to detection of mutations of VWF gene in 15 unrelated patients. Polymerase chain reaction (PCR) and direct sequencing was performed for two functionally regions of the gene (exon 18 and exon 19). This study showed many mutations occurred in males and females patients that is impact  for the change  arrangement the amino acid  causing change VWF protein (as a carrier and protection of F8 protein responsibility for hemophilia A)function, and leading to Von willebrand disease. This study showed several mutations within the VWF gene in fifteen Iraqi patients by the molecular analysis revealed 35 point mutations in this study. These mutations included 15 missense variant and 9 silent mutations, while the rest were intrinsic mutations .These mutations may be related with   Von willebrand disease.Keywords: Von willebrand, Mutation, PCR.
Effects of Purified Bauhinia Variegate Leaves and Methanolic Extract on Diabetic Type-2 Alloxan-Induced in Male Albino Rats Jameelah Kadhim Taher
Journal of Global Pharma Technology Volume 10 Issue 10: 2018
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Abstract

The anti-diabetic effects of purified Bauhinia variegate leaves and methanolic extract were Compared with standard drug Insulin. In vivo studyreveals that animal treated with methanolic extract of Bauhinia variegate show reduction in blood glucose sigificantly, body weight, triglycerides, HDL, cholesterol in Alloxan induced diabetic rats. In Bauhinia variegate treated group, there are no significant increase in urea and creatinine level when compared to the control group which reveals the protective nature of the plant source. It concludes that In vivo antidiabetic activity of whole plant extracts of Bauhinia variegate on normal and Alloxan induced diabetic rats was evaluated and concluded that it possesses anti-diabetic activity. From the above results, it is conducted that purified leaves and methanolic extract of Bauhinia variegate whole plant possess significant hypoglycemic and hypolipidemic effects in alloxan induced diabetic rats.Keywords: Bauhinia variegates, Hypoglycemic, Hypolipidemic, Methanilic extract.
Evaluation of the Bacterial Contamination of Well Waters and the Effects of Leaf Plant Extract of Melia Azedarach on Such Contamination Saba Riad Khudhaier Al-Taei
Journal of Global Pharma Technology Volume 11 Issue 04.
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Abstract

This work has been conducted to examine bioactivity of certain species of bacteria in well water samples collected from eight wells in Yusufiyah area southern Baghdad and to assess the effects of plant leaf extraction of Melia azedarach on these bacteria. Bacteriological properties such as total plate count cfu/ml, MPN/1.ml, Fecal Coliform, Fecal Streptococci, E. coli and Pseudomonas aeruginosa of well water samples have been evaluated in July and January 2017. Total numbers of bacteria in July was varied from 210 to 6070 CFU / ml while the total numbers of bacteria in January was ranged from 115 to 1300 CFU / ml. The local and international specifications MPN reached the upper limit for the preparation of the total coliform count in a manner most likely CFU / ml> 1600 (MPN) and have a minimum CFU / 900ml in July but in January, it has reached the upper limit of the number of total coliform manner that count the most likely CFU / 100 ml> 1550 (MPN) and minimum CFU / 100 ml 100 hence conclude that 87.5% of water wells in July and January. Well waters indicators fecal coliform bacteria and E. coli and fecal Streptococci contamination. However, the contamination with fecal coliform bacteria was considered as an acceptable because they are not exposed to any type of chlorination before consumption by users. The dried plant leaves of the Melia azedarach were extracted by using methanol alcohol and the bioactivity of this extract was evaluated with isolated bacteria. The results have showed that the effects of the extract depend on bacteria species and the extraction concentration used. However, it was found that the extract had significant bioactivity on bacterial contaminated water and such ability of methanol alcoholic extract may be due to the content of steroidal, phenols and tannins compounds in the examined plant leaves being very effective against microbes.Keywords: Bioactivity,   Melia azedarach, Extraction, Bacterial contamination, Well water.
Effect of Ozone on Antagonistic Activity of Lactobacilli Anzor A. Ismailov
Journal of Global Pharma Technology Volume 12 Issue 02 (2020) Feb. 2020
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Abstract

This article describes study results that confirm the effect of ozone on the antagonistic effect of Lactobacillus plantarum 8P-A3 against Bacillus subtilis, Escherichia coli and Candida albicans test cultures. A stable (within 48 hours) dose-dependent effect on lactobacilli antagonistic activity was proven; it was maintained in all used ozone concentrations (from ˂0.29 mg/L, 0.29 mg/L, 0.38 mg/L). It was found that the intensity of lactic acid formation which is the most important criterion for lactobacilli antagonistic activity is significantly higher at the ozone concentration of ˂0.29 mg/L than in control samples; herewith the bacterium retains the properties of a strong antagonist. Level of lactic acid at the doses of 0.29 mg/L and 0.38 mg/L is reduced in comparison to control samples, and lactobacilli can be characterized as weak antagonists.Keywords: Antagonistic activity of lactobacilli, Antagonistic factors, Ozone, Effect of ozone on microorganisms, Lactic acid.

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