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Jurnal Sain Veteriner

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Published by Universitas Gadjah Mada

ISSN : 012660421 EISSN : 24073733 DOI : -

Core Subject : Health,

Veterinary

Arjuna Subject : -

Articles 824 Documents

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Comparison of Toxoplasma gondii Cysts Predilection in the Heart and Diaphragm of Pig in Bali I Wayan Wirata
Jurnal Sain Veteriner Vol 32, No 2 (2014): DESEMBER
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

A study was conducted on Toxoplasma gondii in the heart and diaphragm of pigs in Bali. The study was aimed to compare the predilection of Toxoplasma gondii cysts in the hearts and diaphragms of pigs and compared to the number of T. gondii cysts per gr from the hearts and diaphragm sof infected pigs. Pigs were examined as many as 171. Each organ was examined by digestion method in order to identify the presence of T. gondii cysts in these organs. The parameters measured were the percentages and numbers of T. gondii cysts which werefound in each organs. The results showed that 9 of pigs were infected with T. gondii (5,26%) which in the hearts and diaphragms were 4.09% and 1.17%, respectively. The average number of T. gondii cysts per gr of the heart and diaphragm were 30.86 ± 21.38 and 24, respectively.

Chemistry and Microbiology Qualities of Hard Candy-Green-Sirih Leaves (Piper betle L.) As Cattle Feed Additive Anika Prastyowati; Lorensia Maria Ekawati Purwijantiningsih; Fransiskus Sinung Pranata
Jurnal Sain Veteriner Vol 32, No 2 (2014): DESEMBER
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Cattle diseases caused by bacterial infection and unqualified feed can decrease production of beef which people consume more in Indonesia. This research objective is to know quality of hard candy as cattle feed additive including chemistry and microbiology characteristics. Research steps consist of material preparation test, making sirih leaf extract, making hard candy, chemistry tests (The levels of water, ashes, reduction sugar, sacarose and atsiri oil, respectively) and microbiology test (Total count test, and yeast and Fungi Test). Theresults showed that hard candy made fulfill SNI (Indonesia National Standar) considered of water level, ashes level, sacarose level, total count, and yeast fungi level. Atsiri oil level before sirih leaves are made into hard candy is 0,405% and it still exists in hard candy with increasing atsiri oil level, 0,1333%.

Production Of Crude Aspergillus Fermentation Extract To Enhance The Quality Of Feed As A Productivity Booster Of Super Chicken Aris Munandar; Vembriarto Jati Pramono
Jurnal Sain Veteriner Vol 32, No 2 (2014): DESEMBER
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Chicken meat is one of the most popular products that is really interesting for the consumers.The aim of this study was to produce feed supplement that can be used to improve food digestibility and productivity as well in local super chicken. In this research, Group I (control group) was given feed and drink. ad libitum , Group II was given feed and drink ad libitum with the addition of fermented Aspergillus niger a dose of 4 ml / L of drinkingwater. Group III was given feed and drink ad libitum with the addition of fermented Aspergillus oryzae a dose of 4 ml / L of drinking water for 60 days, and then weight gain, food conversion rate as well as calcium and phosphate levels in the blood were observed. The results were analyzed using analysis of variance and showed that there were weight gain differences between the control and treatment groups of A. niger. There is no weight gain difference between the control and feed supplement groups . The blood calcium and phosphate levelsbetween the control and feed supplement groups were also not different.

The Effect of Sinbiotic Supplement as Replacement for Antibiotic Growth Promoter on Growth and Size of Intestinal Villi in Broiler Chicken Muhammad Arifin; Vembriarto Jati Pramono
Jurnal Sain Veteriner Vol 32, No 2 (2014): DESEMBER
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Over the past few decades, antibiotics have been used as growth promoters in poultry diet to improve animal performance and to obtain economic benefits. The aim of this study was to know the effect of sinbiotic derived from Bacillus subtilis as probiotic and Saccharomyces cerevisiae cell wall combination as one of antibiotic growth promoters (AGP) in broilers. Group 1 (control), group 2 (AGP), and group 3 (sinbiotic). Broilers from each group were necropsied for histological preparation and the length and width of intestinal villus were measured histologically. The data were taken from feed, body weight, Feed Conversion Ratio (FCR), and the length and width of intestinal villi. Analysis of Variance were used to analize the data. The results of the length and width of the duodenum, jejunum, and colon villi in group 3 and group 1 were showed significant differences (P <0.05) as well as a comparison between group 3 and group 2. Statistical analysis of weight gain during 5 weeks was showed significant differences (P <0.05) between groups except at week 2 and 3. Analysis of FCR was showed that there were significant differences (P <0.05) between groups on a weekly basis. It was concluded that sinbiotic increase the length and width of intestinal villi, increase the body weight gain ofchicken, and reduce the value of FCR, therefore sinbiotic can be used as an alternative replacement for Antibiotic Growth Promoter (AGP).

Effect Of Keren Fruit Extract (Muntingia calabura) On Blood Glucose Levels Of Rats (Rattus novergicus) Which Induced By Streptozotocin (STZ) Vembriarto Jati Pramono; Rahmad Santosa
Jurnal Sain Veteriner Vol 32, No 2 (2014): DESEMBER
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Diabetes mellitus is reveal various symptoms that characterized by increasies of blood glucose levels (hyperglycemia) due to insulin deficiency. Kersen fruit (Muntingia calabura) is thought to contain antidiabetic substance such as ascorbic acid, fiber, beta-carotene, riboflavin, thiamine and niacin. The study was conducted to determine the potential of kersen fruit in decreasies blood sugar levels. The group divided into group I (negativecontrol), group II (positive control), group III, IV, and V were given kersen fruit extract 100 mg/kg BW, 200 mg/kg BW, and 400 mg/kg BW respectively. Rats of groups I-V were induced with streptozotocin (STZ). Blood sugar values were analyzed using Analysis of Variance Repeated method (Repated ANOVA). The results showed blood glucose levels before treatment, week-0, and week-2 in the group I (133 mg/dL, 164 mg/dL, 105 mg/dL), group II (136 mg/dL, 362 mg/dL, 431 mg/dL), group III (133 mg/dL, 513 mg/dL, 109 mg/dL), group IV (100 mg/dL, 376 mg/dL, 153 mg/dL), and group V (83 mg/dL, 225 mg/dL, 169 mg/dL). Respectively based on statistical analysis showed a significant decreasies of blood sugar levels of the group III so that kersen extract with the dose of 100 mg / kg has the potential to antidiabetic.

The Effects of Hymeniacidon sp. Sponge Extract to The Level of Parasitemia Trypanosoma evansi in BALB/c Mice (Mus musculus L.) Maya Ekaningtias
Jurnal Sain Veteriner Vol 32, No 2 (2014): DESEMBER
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Trypanosoma evansi is a blood protozoa which has infected many livestock like cow, horse, and buffalo and caused disease so called surra. Disease medication of surra depends on the latest invention of trypanocidal medicine, but must of synthetic medicine has become resistance. Therefore, another alternative has been developed using natural materials. Sponge is a kind of oceanic organism which well known for producing secondary metabolite which has biology activity. One of them is Hymeniacidon sp. which known containingterpenoid bioactive compound. The purpose of this research is to study the effect of Hymeniacidon sp. sponge extract to the level of parasitemia T. evansi in Balb/C mice (Mus musculus L.) and to find out the most optimum Hymeniacidon sp. sponge extract to prevent T. evansi growth. This research has used 40 male Balb/C mice, age 2-3 months, weight ± 23-30 grams. The research subject was divided in to 8 groups (each group contain 5 mice).The classification consists of negative control DMSO, negative control of sponge extract, positive control which is infected by T. evansi and 5 groups treatment T. evansi accompanied with sponge extract given. Sponge extract concentration which was used were 50 mg/kg of BB, 100 mg/kg of BB, 200 mg/kg of BB, 400 mg/kg of BB, and800 mg/kg of BB, respectively. Mice were injected intraperitoneally with 0,2 ml of blood containing 103 of T. evansi. Sponge extract was given orally at 24 hours after T.evansi infection and conducted during 4 days. Then, film blood smear is made, observed by microscope and level of parasitemia T.evansi in blood was calculated.Result of this research indicated that Hymeniacidon sp. kloroform sponge extract concentration variation significantly decrease to the level of parasitemia T. evansi. The most effective concentration is 400 mg/kg of BB with reactivity equal to 98,71% on the fourth days research.

Histopathological Features of Duodenal Coccidiosis in the Probiotic Broiler Chickens Aven B Oematan; Damai Kusumaningrum
Jurnal Sain Veteriner Vol 32, No 2 (2014): DESEMBER
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

This aim of research was to know histopathology lesions of broiler duodenum in chicken coccidiosis with probiotic administrasion. Sixty six DOC of broiler, strain Arbor acress, male sex were divided into 5 groups of twelve each. Chickens in Groups I, II, III, IV and V were given standard food, standard food+probiotic, standard food+1000 oocysts, standard food+probiotic+1000 oocysts and standard food+probiotic+ 3000 oocysts,respectively. Probiotic was given starting at the age of 8 days by sprayed in food with the dose 2 ml/ 250 ml water/ 1 kg food. At the age of 38 days, chickens were infected orally by given 1000 oocysts for group III and IV, and 3000 oocysts for group V. Group I and II were euthanazed at the age of 39 days, group IIIA, IVA, VA at 5 days postinfection, group IIIB, IVB, VB at 6 days post infection, and group IIIC, IVC, VC at 7 days post infection. Histopathologic lesions of the duodenums were analyzed with qualitative analyze. Results showed that infection without probiotic area were found histopathologic changes of duodenum in the form of congestion, hemorrhages, schizonts and macrogametes representing of Eimeria sp stadium which caused coccidiosis. Treatment infection with probiotic were found histopathologic changes in the duodenum, such as congestion,hemorrhages, necrotic epithelial cells, and also schizonts, microgametes and macrogametes representing of Eimeria sp stadium which caused coccidiosis. Probiotic in the present study did not have any effect for histopathologic changes in the duodenum in the broiler chickens infected coccidiosis.

Total Fat, Calcium (Ca), Iron (Fe) and Zinc (Zn) Contents During Ecdysis process in the Crabs (Scylla serrata, Forsskal) Sri Swasthikawati; Rarastoeti Pratiwi; Trijoko .
Jurnal Sain Veteriner Vol 32, No 2 (2014): DESEMBER
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Crab is one of the most abundant marine animals in Indonesia. Crabs not only rich of nutrient, but also have commercial value. Today, there is an innovation in crab aquaculture use ecdysis mechanism, known as soft shell crab. However, the limited studies about biochemical and nutritional composition of this crab remain to be explored. The aims of this research were to understand total fat, calcium, iron, and zinc contents of crab (Scyllaserrata) during ecdysis process until being soft shell crab comparing to the hard shell crab from the same species. This research was done using crab Scylla serrata, male, weighing ± 150-180 gr, completely randomized design with four variations of treatment, during 8 days, 16 days, soft shell crab, and control, repeated 3 times. Samples from each treatment were grown and were prepared for the chemical analysis. Total fat was measured usingSoxhlet method with chloroform and methanol (1:2, v/v) as solvent. Atomic absorption spectrometer was used to measure calcium, iron, and zinc contents. Then, the result were analyzed using one way Anova and Duncan 5 %. The results showed that the total fat, calcium and zinc contents of soft shell crab Scylla serrata were higher and significantly different with three other treatments. It could be concluded that the ecdysis process, raised total fat, calcium and zinc contents of crab Scylla serrata. The total fat, calcium, and zinc contents of soft shell crab Scylla serrata was higher than that of hard shell crab from the same species.

North Kalimantan Is Free from EMS (Early Mortality Syndrome) in the Shrimps Sab Lestiawan; Nugroho Sasongko Jati; Hermas Wiro
Jurnal Sain Veteriner Vol 32, No 2 (2014): DESEMBER
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

The acute hepatopancreatic necrosis syndrome (AHPNS) or also known worldwide as early mortility syndrome (EMS) is very dangerous for the shrimps industry since it really strikes the shrimps by larvae size. The EMS attacks shrimps at 20 to 30 days of age and at 40 days of age as well. All the shrimps that are being infected will be dead righ away. The EMS has caused the significant economic losses in the shrimps industry due to the mass mortality of the shrimps in Vietnam (in the years of 2010- 2012). It also occurred ad spread around China (2009), Vietnam (2010), East Malaysia (2010), East of Thailand (2012) until approaching the edge of Cambodia (2013). It has been reported that the EMS is likely due to Vibrio parahaemolitycus that are infected by certain virus (phage), and then the bacteria releasing very toxic substances (toxin). Efforts has been being done by the Indonesian government, especially in the Fish Quarantine Office, Kls II, Tarakan in the form of prohibition of entry of live and/or frozen shrimps importing from the EMS-outbreak country. Besides that, monitoring and laboratory works has also been performed routinely and periodically. The results proves thatNorth Kalimantan is free of AHPNS (EMS).

Infestation of Luke Worm (Fasciola sp) and Stomach Worm (Paramphistomum sp) on Adult Bali Cattle in Tenayan Raya Pekanbaru Municipality Fahrur Rozi; Jully Handoko; Rahmi Febriyanti
Jurnal Sain Veteriner Vol 33, No 1 (2015): JUNI
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

This study was conducted at the Laboratory of Parasitology, Veterinary Investigation Center Regional II,Bukittinggi in January until February 2013. Bovine fecal samples of Bali cattle were taken in the District of Tenayan Raya Pekanbaru Municipality. This study was aimed to determine the prevalence of paramphistomiasis and fascioliasis of adult males and females Bali cattle and the number of eggs worm per gram of feces as well. A total of 232 fecal samples were tested by Parfit and Banks, and McMaster. Results showed that the prevalence of Fasciola spp. in males Bali cattle and cows was 49.02% and 60.71%, respectively, whereas the prevalence of Paramphistomum spp. in males Bali cattle and cows was 46.07% and 50.00%,respectively. Result count of the average number of worm eggs using Mcmaster in the fecal samples of Bali cattle in males and females was 205.88 eggs/g and 267.85 eggs/g, respectively. This study indicated that the eggs worm counts per gram feces could be classified into mild infection.

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