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Ayyub Harly Nurung
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Editor's Address: 3rd Floor Laboratory Faculty of Pharmacy, 2nd Campus of UMI: Jl. Urip Sumoharjo km. 5 , Makassar, South Sulawesi, Indonesia
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Journal Microbiology Science
Published by Universitas Muslim Indonesia
ISSN : 2808392X     EISSN : 28083911     DOI : https://doi.org/10.56711/jms
Core Subject : Health, Science,
Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology
Journal Microbiology Sciences (J. Mic. Sciences) receives writing in the form of research results, scientific studies and brief descriptions relating to Microbiology, such as Molecular and Microbial Epidemiology Clinical Microbiology Medical Microbiology Pharmaceutical Biotechnology and others related to Microbiology Sciencee
Arjuna Subject : Imunologi dan Mikrobiologi (semua kategori) - Mikrobiologi dan Bioteknologi Terapan
Articles 67 Documents
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Antibacterial Activity of Endophytic Fungi from Bidara Roots Against Bacteria that Cause Skin Infections Nurdayani, Sitti; Fitriana, Fitriana; Amirah, Sitti
Journal Microbiology Science Vol 4, No 1 (2024)
Publisher : Fakultas Farmasi, Universitas Muslim Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56711/jms.v4i1.1001

Abstract

Endophytic fungi are fungi that grow and colonize plant tissues and are capable of producing bioactive compounds similar to those produced by their host. This study aims to determine the concentration of endophytic fungal isolates from Bidara roots (Ziziphus mauritiana Lam) that have antibacterial activity against bacteria that cause skin infections using the agar diffusion method. The research method begins with the rejuvenation of IFAZ-6 isolates, and microscopic and macroscopic observations are carried out. The isolates were fermented using Maltose Yeast Broth medium at 200 rpm for 21 days. The fermentate was extracted with an ethyl acetate solvent to obtain a thick extract. The tests carried out were minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC), and antibacterial activity tests carried out using the agar diffusion method. The results of the antibacterial activity test showed that the IFAZ-6 isolate extract at a concentration of 800 ppm showed antibacterial activity against Staphylococcus aureus, Staphylococcus epidermidis, Pseudomonas aeruginosa, and Propionibacterium acnes. The statistical analysis results showed that the extract of isolate IFAZ-6 against Staphylococcus aureus and Staphylococcus epidermidis at concentrations of 800-1600 ppm and 1600–3200 ppm exhibited the same antibacterial activity. The bacterium Pseudomonas aeruginosa showed the same antibacterial activity at all tested concentrations, and the bacterium Propionibacterium acnes at concentrations of 1600–3200 ppm also exhibited the same antibacterial activity.
Optimization Time for Antibacterial Production of Endophytic Fungi Isolated from Bidara Root (Ziziphus mauritiana Lam.) Annisa, Khusnul; Fitriana, Fitriana; Amirah, Sitti
Journal Microbiology Science Vol 4, No 1 (2024)
Publisher : Fakultas Farmasi, Universitas Muslim Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56711/jms.v4i1.997

Abstract

Research on optimization time for antibacterial production of endophytic fungal isolated from bidara root (Ziziphus mauritiana Lam.) has been conducted, with the aim of determining the optimum production time for antibacterial compounds. Endophytic fungi are a group of fungi that live within plant tissues without causing harm to their host plants. In this research, the roots of bidara were isolated and purified using PDA media, isolates were followed by screening endophytic fungi isolates against test bacteria. The results of the endophytic fungal isolation from the bidara roots yielded 10 endophytic fungal isolates. The screening test against the test bacteria showed the largest inhibitory zone for IFAZ-6. Subsequently, the growth curve of these isolates was determined through fermentation using MYB media using a shaker speed of 200 rpm at a temperature of 25-28°C for 27 days. Then, mycelia and supernatant were collected on days 11, 13, 15, 17, 19, 21, 23, 25, and 27 with a 48-hour interval. The mycelia were weighed until a constant weight was obtained, while the supernatant was used to test the activity against the test bacteria using MHA media. The growth curve results indicated a stationary phase on day 21. The antibacterial activity test showed that Isolate IFAZ-6 reached optimum activity on day 27 with an inhibitory zone of 12.33 mm against Salmonella thypi.
Isolation and Identification Endophyte Fungi from Daun Nona Makan Sirih (Clerodendrum thomsoniae) as Antibacteril Against Bacteria Causing Skin Infection Using TLC-Bioautography Hadjar, Aflinda; Fitriana, Fitriana; Asmaliani, Ira
Journal Microbiology Science Vol 4, No 1 (2024)
Publisher : Fakultas Farmasi, Universitas Muslim Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56711/jms.v4i1.1034

Abstract

Nona makan sirih (Clerodendrum thomsoniae) contains secondary metabolites that have the potential as antibacterials. The TLC-Bioautography method will determine the antibacterial activity and chemical components of endophytic fungal isolates of nona makan sirih. This research involved the isolation of endophytic fungi from leaves of nona makan sirih using PDAC media, followed by purification and macroscopic and microscopic tests. In addition, a screening test its effect againts pathogenic bacteria. The chosen isolates were fermented for 21 days. Then supernatant and mycelia were seperated. The supernatant was extracted by dissolving ethyl acetate using the liquid-liquid ectraction method. Then extract obtained was processed by TLC with the mobile phase of chloroform:methanol (8:1) and TLC-Bioautography was carried out on bacterial tests and chemical components. The results of isolation and purification obtained as many as 13 isolates. Screening test results with IFDNMS codes 6, 8 and 11 showed activity against the test bacteria. TLC-Bioautography result isolate 6 obtained 5 spots with an Rf value of 0.89; 0.65; 0.45; 0.23; 0.05 was active against all test bacteria. Isolate 8 obtained 5 spots with an Rf value of 0.89; 0.65; 0.05 was active against all tested bacteria, while 0.45 was active against P.acnes, S.aureus, and S.epidermidis bacteria and 0.23 against S.aureus bacteria. Isolate 11 obtained 5 spots with an Rf value of 0.89; 0.61; 0.45; 0.23; 0.05 against all test bacteria. The findings indicate that flavonoids and phenolics are chemical components with antibacterial activity.
Production of Antibiotics from Endophyte Fungi Isolates of Bidara Plants (Ziziphus mauritina L.) Isolates Codes IFAZ-6, IFBZ-6, IFDZ-8 With Variations in Carbon Sources Fitriana, Fitriana; Nurung, Ayyub Harly; Adnan, Afdina Putri
Journal Microbiology Science Vol 4, No 2 (2024)
Publisher : Fakultas Farmasi, Universitas Muslim Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56711/jms.v4i2.1149

Abstract

Research has been carried out on antibiotic production from endophytic fungal isolates coded IFAZ-6, IFBZ-6, and IFDZ-8 from bidara plants (Ziziphus mauritina L.) with variations in carbon sources. This research aims to determine antibiotic production with variations in carbon sources. The first stage was the purification of each selected endophytic fungal isolate. The endophytic fungal isolate was inoculated into the fermentation medium using a variety of carbon sources, namely glucose, fructose, and galactose for 21 days. The antibiotic activity was carried out using the agar diffusion method against Staphylococcus aureus, Pseudomonas aeruginosa, and Escherichia coli bacteria. The results obtained showed that the glucose carbon source was better in producing antibiotic compounds than the galactose carbon source in endophytic fungal isolates coded IFAZ-6, the glucose carbon source was better in producing antibiotic compounds than the fructose and galactose sources in endophytic fungal isolates coded IFBZ-6, and the fructose carbon source is better at producing antibiotic compounds than the galactose source in endophytic fungal isolates coded IFDZ-8.
Exploring The Antibacterial Potential of Aqueous and Ethanol Extracts from Bawang Dayak (Eleutherine palmifolia (L.) Merr) Against Skin Infectious Bacteria Through KLT-Bioautography Rusli, Rusli; Nuryanti, Siska; Putria, Yashifa Anandita
Journal Microbiology Science Vol 4, No 2 (2024)
Publisher : Fakultas Farmasi, Universitas Muslim Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56711/jms.v4i2.1131

Abstract

Dayak onion plants (Eleutherine palmifolia (L.) Merr) have chemical compounds that have potential as antibacterials such as alkaloids, glycosides, flavonoids, phenolics, steroids and tannins. This study aims to determine the antibacterial activity of water extracts and ethanol extracts of dayak onion bulbs (Eleutherine palmifolia (L.) Merr). This research was conducted with the stages of sample extraction, screening of test bacteria, bioautogram profile testing and antibacterial activity by KLT-Bioautography method. Screening test results at a concentration of 1% against test bacteria Propionibacterium acnes, Pseudomonas aeruginosa, Staphylococcus aureus and Staphylococcus epidermidis were able to inhibit the growth of test bacteria. Then continued with KLT-Bioautography testing using n-hexan: ethyl acetate (2: 1) eluent. The KLT-Bioautography test results showed that there was 1 active spot with an Rf value of 0.63 in the water extract and an Rf value of 0.60 in the ethanol extract against Pseudomonas aeruginosa, Propionibacterium acnes, Staphylococcus aureus, and Staphylococcus epidermidis bacteria. Based on the research, water extract and ethanol extract of dayak onion bulb (Eleutherine palmifolia (L.) Merr) have potential as antibacterial.Keywords: Antibacterial, KLT-Bioautography, Dayak Onion Bulbs
Antibacterial Activity of Endophytic Fungi Isolated from Parang Romang (Boehmeria virgata (G.Forst) Guill) Leaves Against Gastrointestinal Pathogens Rusli, Rusli; Nurung, Ayyub Harly; Sandyna, Muh. Ivan
Journal Microbiology Science Vol 4, No 2 (2024)
Publisher : Fakultas Farmasi, Universitas Muslim Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56711/jms.v4i2.1081

Abstract

Parang romang (Boehmeria virgata (G.Forst) Guill) is a plant traditionally used for treating various digestive tract infections. However, obtaining bioactive compounds directly from the plant requires significant biomass. Therefore, endophytic fungi, sourced from specific plant parts, are explored for their ability to produce similar bioactive compounds. The research examined the potential antibacterial activity of endophytic fungi isolated from parang romang leaves against common gastrointestinal pathogens. The methods used in was, characterization of endophytic fungal isolates, and antagonistic activity was evaluated using the agar diffusion method. The results showed that the endophytic fungal strains, namely isolates IFBV 6, IFBV 7, and IFBV 10 could inhibit the bacteria Escherichia coli, Salmonella typhi, Shigella dysenteriae, and Vibrio cholera. With an average zone of inhibition for each strains isolate against Esherichia coli respectively 11.53 mm, 10.49 mm, and 14.85 mm; Salmonella typhi 14.47 mm, 8.66 mm, and 15.51 mm; Shigella dysenteriae 22.28 mm, 13.76 mm, and 15.33 mm; and Vibrio cholera 16.91mm, 11.94mm, 15.16mm. In conclusion, isolated endophytic fungal strains hold promise as potential as antibacterialn agents.
Synergistic Antibacterial Effects of Areca Nut Seed (Areca catechu L.) and Binahong leaf (Anredera cordifolia (Ten.) Steenis) Extracts Against Propionibacterium acnes Nuryanti, Siska; Nurung, Ayyub Harly; Saputra, Muh. Satrio Surya; Musfatir, Harvanita; Umaiyah, Ummi Putri
Journal Microbiology Science Vol 4, No 2 (2024)
Publisher : Fakultas Farmasi, Universitas Muslim Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56711/jms.v4i2.1150

Abstract

Areca nut (Areca catechu L.) seeds has antibacterial properties due to their alkaloid, flavonoid, tannin, saponin, and polyphenol compounds. Binahong leaves (Anredera cordifolia (Ten.) Steenis) contain flavonoids, polyphenols, saponins, and alkaloids that also act as antibacterial agents. This study aimed to assess the antibacterial activity and synergistic effects of combining extracts from areca nut and binahong leaves against Propionibacterium acnes. The Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) of P. acnes were evaluated for the ethanol extracts of areca nut and binahong leaves. The MIC and MBC values 400 ppm and 1.600 for extract ethanol single areca nut and 50.000 ppm and 100.000 ppm for binahong leaves. The MIC and MBC values for the combination 400/12.500 ppm and 1.600/781,25 ppm for areca nut and 50,000/800 ppm and 100,000/100 ppm for binahong leaves, respectively. The synergy between the two extracts was determined using the Fractional Inhibitory Concentration Index (FICI). The FICI value of 1. P. acnes indicates an indifferent effect (1 < FICI ≤ 4).
Antibacterial Activity of Miana Leaf Ethanol Extract (Coleus scutellarioides (L) Against Some Pathogenic Bacteria Using The TLC Method - Bioautography Fitriana, Fitriana; Harmunanto, Kartika Hatari; Zulkarnain, Iskandar
Journal Microbiology Science Vol 4, No 2 (2024)
Publisher : Fakultas Farmasi, Universitas Muslim Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56711/jms.v4i2.1137

Abstract

Miana (Coleus scutellarioides (L.). is one of the plants that grows in areas with rather moist or slightly watery soil conditions, this plant is often consumed by people in Indonesia and is believed to be a medicinal plant. Empirically, miana leaves are used as antibiotics because they are known to have antibacterial activity. The purpose of this study was to determine the bioautogram profile of miana leaf ethanol extract against several pathogenic bacteria using the TLC-Bioautography method. As for the results of this study, ethanol extract of miana leaves (Coleus scutellarioides (L.)) has the potential as antibacterial against bacteria Staphylococcus aureus, Escherichia, Pseudomonas aeurigosa, and Streptococcus mutans, where for Staphylococcus aureus bacteria there are 2 active spots with Rf values of 0, 98, 0.32 and Streptococcus mutans bacteria there are 3 active spots with Rf values of 0.98, 0.76, and 0.69 respectively. As for Escherichia bacteria, there is 1 active spot with an Rf value of 0.81 and Pseudomonas aeurigosa bacteria there are 3 active spots, namely 0.87, 0.49, and 0.29, respectively.
Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) of Water and Ethanol Extractsof Eleutherine palmifolia (L.) Merr. Against Staphylococcus aureus and Psedomonas aureginosa Rusli, Rusli; Nurung, Ayyub Harly; Erwing, Erwing
Journal Microbiology Science Vol 4, No 2 (2024)
Publisher : Fakultas Farmasi, Universitas Muslim Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56711/jms.v4i2.1086

Abstract

Dayak onion bulbs (Eleutherine palmifolia (L.) Merr.) are indigenous herbal plants found in Indonesia, rich in phytochemical compounds such as alkaloids, glycosides, flavonoids, phenolics, steroids, and tannins. This study investigates the Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) of water and ethanol extracts of Dayak onion bulbs against the bacteria Staphylococcus aureus and Pseudomonas aeruginosa. The water extract of Dayak onion bulbs was obtained by boiling using water, while the ethanol extract was obtained by maceration using 96% ethanol solvent. MIC and KBM were determined using the agar diffusion method. The results showed that water extract exhibited an MIC of 0.4% and MBC of 1.6% against both Staphylococcus aureus and Pseudomonas aeruginosa. Meanwhile, the ethanol extract demonstrated an MIC of 0.4% against Staphylococcus aureus and Pseudomonas aeruginosa bacteria with an MBC of 0.8% against Staphylococcus aureus bacteria and 1.6% against Pseudomonas aeruginosa bacteria. These findings suggest the potential of Dayak onion bulb extracts as antimicrobial agents against these pathogenic bacteria.
Antibacterial Activity in Ethanol Extract of Kersen Flowers (Muntingia calabura L.) Against Bacteria Causing Gastrointestinal Tract Infections Using the Agar Diffusion Method Maryam, St.; Fitriana, Fitriana; Annisa, Nurfiqah
Journal Microbiology Science Vol 4, No 2 (2024)
Publisher : Fakultas Farmasi, Universitas Muslim Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56711/jms.v4i2.1157

Abstract

The increasing prevalence of antibiotic resistance necessitates the exploration of alternative treatments such as medicinal plants. This study evaluates the antibacterial potential of Kersen flowers (Muntingia calabura L.) against pathogens causing gastrointestinal infections. Aimed at determining the antibacterial effectiveness of ethanol extracts from Kersen flowers, the research tests the activity against Escherichia coli, Salmonella typhi, Vibrio cholerae, and Shigella dysenteriae. Employing the agar diffusion method, various concentrations of the extract (0.05% to 10%) were analyzed. Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) were also assessed to identify the lowest effective concentrations. The ethanol extract demonstrated significant antibacterial activity; notably, the largest inhibition zone measured 30.74 mm at a concentration of 10%. Effective MICs were as low as 0.1% for all tested bacteria, while MBCs ranged from 0.1% to 0.4%. These results suggest that ethanol extracts of Kersen flowers exhibit potent antibacterial properties against major gastrointestinal pathogens, supporting further exploration of these extracts as a natural antibacterial treatment and offering a potential alternative to conventional antibiotics.

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