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Journal Microbiology Science
Published by Universitas Muslim Indonesia
ISSN : 2808392X     EISSN : 28083911     DOI : https://doi.org/10.56711/jms
Core Subject : Health, Science,
Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology
Journal Microbiology Sciences (J. Mic. Sciences) receives writing in the form of research results, scientific studies and brief descriptions relating to Microbiology, such as Molecular and Microbial Epidemiology Clinical Microbiology Medical Microbiology Pharmaceutical Biotechnology and others related to Microbiology Sciencee
Arjuna Subject : Imunologi dan Mikrobiologi (semua kategori) - Mikrobiologi dan Bioteknologi Terapan
Articles 75 Documents
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Volume 5(1) 2025 Science, Journal Microbiology
Journal Microbiology Science Vol 5, No 1 (2025)
Publisher : Fakultas Farmasi, Universitas Muslim Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56711/jms.v5i1.1339

Abstract

Antibacterial Activity of Etanol Extract of Pinang (Areca catechu L.) Clup against Streptococcus mutans and Porphyromonas gingivalis Nuryanti, Siska; Herwin, Herwin; Rahmayanti, Hani Rahmayanti
Journal Microbiology Science Vol 5, No 1 (2025)
Publisher : Fakultas Farmasi, Universitas Muslim Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56711/jms.v5i1.1178

Abstract

Areca nut (Areca catechu L.) is a plant that has many uses and can be used for traditional medicine. Areca nut skin can be used to treat indigestion (dyspepsia), edema and beriberi due to low urine output. This study aims to determine the antibacterial activity of ethanol extract of areca nut peel against the test bacteria Streptococcus mutans and Porphyromonas gingivalis. In this research, the areca nut peel simplicia was extracted using the maceration method with 96% ethanol solvent, after which a liquid ethanol extract was obtained and then evaporated using a rotary vacuum evaporator to obtain a thick extract. After the extract is thick, a Minimum Inhibitor Concentration (MIC) test and a Minimum Bactericide Concentration (MBC) test are carried out. The results obtained in the Minimum Inhibitor Concentration (MIC) test and Minimum Bactericide Concentration (MBC) test on Streptococcus mutans and Porphyromonas gingivalis bacteria were 0.5%. Antibacterial activity test using the agar diffusion method was obtained, for Streptococcus mutans bacteria, the largest inhibitory zone diameter at a concentration of 16% was 12.58 mm and for Porphyromonas gingivalis bacteria at a concentration of 16%, namely 10.93 mm. Based on research, ethanol extract of areca nut peel has antibacterial potential.
Antimicrobial Activity of Ethanol Extract of Pinang Seed (Areca catechu L.) Against the Growth of Candida albicans and Malassezia furfur Fungi Using the Agar Diffusion Method Naid, Tadjuddin; Saputri, Febrianti Ayu; Nuryanti, Siska
Journal Microbiology Science Vol 5, No 1 (2025)
Publisher : Fakultas Farmasi, Universitas Muslim Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56711/jms.v5i1.1229

Abstract

Pinang seeds (Areca catechu L.) are known for their various benefits, including antimicrobial properties. This study aims to evaluate the antimicrobial activity of ethanol extract from pinang seed against the growth of Candida albicans and Malassezia furfur fungi using the agar diffusion method. The research involved extracting the pinang seed using maceration with 96% ethanol, followed by evaporation using a rotary vacuum evaporator to obtain a concentrated extract. Antimicrobial activity was tested across concentrations of 32%, 16%, 8%, 4%, 2%, 1%, 0.5%, and 0.1%. The results showed that at a concentration of 32%, the ethanol extract produced the largest inhibition zones: 27.78 mm against Candida albicans and 15.51 mm against Malassezia furfur, both categorized as very strong activity. These findings suggest that ethanol extract of pinang seed has significant potential as an antimicrobial agent, particularly against fungal pathogens.
Antibacterial Activity of Ethanol Extract of Cocoa Leaves (Theobroma cacao L.) Against Gastrointestinal Infection Bacteria Tract Infection by Agar Diffusion Method Sya’baniyah Herman, Nur Fajriah; Fitriana, Fitriana; Maryam, St.
Journal Microbiology Science Vol 5, No 1 (2025)
Publisher : Fakultas Farmasi, Universitas Muslim Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56711/jms.v5i1.1218

Abstract

The cocoa plant (Theobroma cacao L.) leaves contain secondary metabolites, including flavonoids, saponins, and tannins, known for their potential antibacterial properties. This study aims to evaluate the antibacterial activity of the ethanol extract of cocoa leaves (Theobroma cacao L.) against bacteria that cause gastrointestinal infections using the agar diffusion method. The study assessed the Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) of the extract. The results showed that the ethanol extract exhibited significant antibacterial activity, particularly at concentrations of 2.5%, 5%, and 10%, with the highest inhibition zone observed at 10% concentration. The extract was most effective against Escherichia coli, Salmonella typhi, Shigella dysenteriae, and Vibrio cholerae, with inhibition zones of 15.34 mm, 15.60 mm, 13.81 mm, and 16.43 mm, respectively. These findings suggest that cocoa leaf extract has the potential to be developed as an antibacterial agent against gastrointestinal pathogens.
Characterization of Cellulose-Producing Bacterial Isolates from the Areca Nut (Areca catechu L.) Nuryanti, Siska; Herwin, Herwin; hikma, Nurhikma
Journal Microbiology Science Vol 5, No 1 (2025)
Publisher : Fakultas Farmasi, Universitas Muslim Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56711/jms.v5i1.1179

Abstract

Areca nut peel (Arecha catechu L.), a byproduct of areca nut processing, holds potential as a raw material for bioplastics through cellulose utilization. This study aimed to identify bacterial species in areca nut peel capable of producing cellulose. Employing bacterial isolation and identification methods, including morphological and biochemical tests, four bacterial isolates were obtained. Two isolates, identified as gram-negative, were classified under the genus Acetobacter, and two gram-positive isolates were identified as belonging to the genus Lactobacillus. These findings demonstrate the potential use of cellulose from areca nut peel in bioplastic production.
Antibacterial Activity of the Ethyl Acetate Fraction of Qust Al-Hindi (Saussurea lappa) in Inhibiting Escherichia coli Growth Using the Agar Diffusion Method Mulya, Esti Lestari; Sukmawati, Sukmawati; Fitriana, Fitriana
Journal Microbiology Science Vol 5, No 1 (2025)
Publisher : Fakultas Farmasi, Universitas Muslim Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56711/jms.v5i1.1215

Abstract

The root of the Qust al Hindi plant (Saussurea lappa) has been recognized for its potential in treating infectious diseases. This research aims to evaluate the antibacterial activity of the ethyl acetate fraction of Qust al Hindi roots against digestive tract bacterial infections, specifically targeting Escherichia coli, using the agar diffusion method. The study commenced with an initial screening test at a concentration of 1%, followed by Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) tests at concentrations of 0.25%, 0.5%, 1%, 2%, 4%, 8%, and 16%. The MIC was determined to be 1%, while the MBC was observed at 8%. Further antibacterial activity testing was conducted at concentrations of 32%, 16%, and 8%, with the optimal activity noted at 32%
Antioxidant Activity Analysis of Endophytic Fungal Isolates IFBZ5, IFBZ6, and IFBZ7 from the Stem of Bidara (Ziziphus mauritiana Lam.) Fitriana, Fitriana; Tahir, Masdiana; Hamdhan, Aulia Nurul Insani; Sumardi, Farah Hikmah Nur; Putri, Adinda
Journal Microbiology Science Vol 5, No 1 (2025)
Publisher : Fakultas Farmasi, Universitas Muslim Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56711/jms.v5i1.1193

Abstract

Endophytic fungal isolates coded IFBZ5, IFBZ6, and IFBZ7 derived from the stem of the bidara plant (Ziziphus mauritiana Lam.) have shown potential antioxidant properties. This study aims to test the antioxidant activity of endophytic fungal isolates on the bidara plant stem (Ziziphus mauritiana Lam.) by TLC-Autography method and DPPH (1,1-diphenyl-2-picrylhydrazil) free radical silencing. This study used endophytic fungi isolates code IFBZ-5, IFBZ-6 and IFBZ-7 which were fermented for 21 days using MYB medium which was then liquid-liquid extracted with ethyl acetate (1:1) and evaporated. The fermentate extract obtained was then tested for antioxidant activity qualitatively and quantitatively. Qualitative analysis using TLC plates indicated antioxidant activity in all isolates. Quantitative analysis showed that isolate IFBZ7 exhibited strong antioxidant activity with an IC50 value of 50.120 ppm, while IFBZ5 and IFBZ6 showed very weak activity with IC50 values of 2.482.363 µg/mL and 467.847 µg/mL, respectively. The BHT comparator had an IC50 value of 6.778 µg/mL. This shows that the antioxidant power of endophytic fungal isolates coded IFBZ5, IFBZ6 and IFBZ7 is lower than the antioxidant activity of BHT as a comparison.
Detection of Salmonella Bacteria In Soybean Tempeh Produced In Palekko Village, Takalar Regency Anggraeni, Nur Afni; Naid, Tadjuddin; Nuryanti, Siska
Journal Microbiology Science Vol 5, No 2 (2025): Volume 5(2) 2025
Publisher : Fakultas Farmasi, Universitas Muslim Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56711/jms.v5i2.1231

Abstract

Tempeh is a staple food in Indonesia, known for its nutritional benefits, including 25% protein, 5% fat, 4% carbohydrates, vitamin B12, and rich mineral content. Ensuring the safety and hygiene of tempeh is crucial, especially in preventing contamination by harmful bacteria like Salmonella, which poses serious health risks. This study aimed to detect the presence of Salmonella bacteria in tempeh produced in Palekko Village, Takalar Regency, using Salmonella Shigella Agar (SSA) medium. The method employed was experimental, involving the preparation and dilution of tempeh samples followed by incubation on SSA medium. The results is a no samples detected Salmonella met the Salmonella contamination limit requirements, which were negative/25g.
Antibacterial Activity of Ethanol Extract of Porang Tubers (Amorphophallus muelleri Blume) Against the Growth of Helicobacter pylori ATCC 43504, Shigella dysenteriae ATCC 13313 and Vibrio Cholerae ATCC 14035 Bacteria Nuryanti, Siska; Nurung, Ayyub Harly; Putra, Aryadi Surya
Journal Microbiology Science Vol 5, No 2 (2025): Volume 5(2) 2025
Publisher : Fakultas Farmasi, Universitas Muslim Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56711/jms.v5i2.1358

Abstract

Porang tubers (Amorphophallus muelleri Blume) are known as local plants that contain active compounds such as glucomannan, saponins and flavonoids, which are thought to act as antibacterials. This study was conducted to determine the extent of the ability of ethanol extract of porang tubers to inhibit and kill Helicobacter pylori ATCC 43504, Shigella dysenteriae ATCC 13313, and Vibrio cholerae ATCC 14035 bacteria, by looking at the Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) values. The extract was made using the maceration method using 96% ethanol, then tested for antibacterial properties using the liquid dilution method for MIC and the drop plate method for MBC. The results showed the MIC value for the three test bacteria at a concentration of 50,000 ppm. Meanwhile, the MBC value was not achieved even at the highest concentration, which was 100,000 ppm. Based on the results of this study, it can be concluded that the antibacterial effectiveness of ethanol extract of porang tubers is still relatively weak on Helicobacter pylori ATCC 43504, Shigella dysenteriae ATCC 13313, and Vibrio cholerae ATCC 14035 bacteria.
Isolation of Endophytic Fungus from Bay Leaves (Syzygium polyanthum Wight.) as Antibacterials Against Bacteria Causing Digestive Tract Infections by Bioautography-TLC Fahirah, Jihan; Herwin, Herwin; Handayani, Selpida
Journal Microbiology Science Vol 5, No 2 (2025): Volume 5(2) 2025
Publisher : Fakultas Farmasi, Universitas Muslim Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56711/jms.v5i2.1301

Abstract

Salam (Syzygium polyanthum (Wight) Walp), belonging to the Myrtaceae family, are noted for their potential antibacterial properties targeting bacteria linked to gastrointestinal infections. This study aims to identify endophytic fungus isolates from bay leaves that produce antibacterial compounds effective against bacteria responsible for gastrointestinal infections, utilizing TLC-Bioautography as a method of analysis. The endophytic fungus from bay leaves was isolated, yielding 17 pure isolates. The findings from the antagonist test of pure isolates of endophytic fungus revealed two isolates with the most significant inhibitory zone diameters: IFSP-8 and IFSP-15. These isolates demonstrated effectiveness against Escherichia coli, Salmonella typhi, Shigella dysenteriae, and Vibrio cholerae bacteria. The active isolate was then extracted by liquid-liquid extraction to obtain ethyl acetate extract for the IFSP-8 and IFSP-15 isolates. The antibacterial activity results of the ethyl acetate extract, analyzed through TLC-Bioautography with a chloroform: methanol (9:1) eluent, indicated the values of Rf1 0,76; Rf2 0,56; Rf3 0,47 for the IFSP-8 isolate. In contrast, the IFSP-15 isolate exhibited the values of Rf1 0,81; Rf2 0,69; Rf3 0,56, demonstrating inhibition of bacteria responsible for gastrointestinal infections.

Page 7 of 8 | Total Record : 75

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