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Ayyub Harly Nurung
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Editor's Address: 3rd Floor Laboratory Faculty of Pharmacy, 2nd Campus of UMI: Jl. Urip Sumoharjo km. 5 , Makassar, South Sulawesi, Indonesia
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Journal Microbiology Science
Published by Universitas Muslim Indonesia
ISSN : 2808392X     EISSN : 28083911     DOI : https://doi.org/10.56711/jms
Core Subject : Health, Science,
Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology
Journal Microbiology Sciences (J. Mic. Sciences) receives writing in the form of research results, scientific studies and brief descriptions relating to Microbiology, such as Molecular and Microbial Epidemiology Clinical Microbiology Medical Microbiology Pharmaceutical Biotechnology and others related to Microbiology Sciencee
Arjuna Subject : Imunologi dan Mikrobiologi (semua kategori) - Mikrobiologi dan Bioteknologi Terapan
Articles 75 Documents
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Antibacterial Activity of Keji Beling (Strobilanthes crispa) Ethanol Extract Using TLC – Bioautography Junardin, Nurul Fadhillah; Nuryanti, Siska; Asmaliani, Ira
Journal Microbiology Science Vol 4, No 1 (2024)
Publisher : Fakultas Farmasi, Universitas Muslim Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56711/jms.v4i1.1037

Abstract

Antibacterials are compounds that inhibit the growth of bacteria that are harm humans. Keji Beling (Strobilanthes crispa) contains secondary metabolites such as saponins, flavonoids, terpenoids, polyphenols, and potassium. One part used in traditional medicine is the leaves which can treat various diseases. In this study, the ethanol extract of Keji Beling leaves was tested using TLC-bioautography This study tested the ethanol extract of Keji Beling leaves using TLC-bioautography. The examination begins with a screening for antibacterial activity against several pathogen bacteria, with results that inhibit the growth of Bacillus subtilis, Escherichia coli, Staphylococcus aureus, Staphylococcus epidermis, Pseudomonas aeruginosa, Streptococcus mutans, Salmonella typhii, Shygella dysenteriae, and Vibrio cholera. Test results using the TLC-bioautography method using n-hexane eluent: ethyl acetate (1: 2) found that the spots were active against bacteria Pseudomonas aeruginosa and Staphylococcus aureus Rf value 0.74 and bacteria Bacillus subtilis, Escherichia coli, Salmonella thypii, Staphylococcus epidermidis, Staphylococcus mutans, Shygella dysenteriae, Vibrio cholera with an Rf value of 0.83 The results of the identification of chemical components showed the presence of positive compounds belonging to the class of flavonoids and anthraquinones. The ethanol extract of keji beling leaves contains a class of flavonoid compounds that have the potential as antibacterial agents with an Rf value of 0.83
Evaluation of Ethanol Extract Tapak Dara Leaf (Catharanthus roseus L. ) for Antibacterial Activity against Skin Pathogens Alhijrah, Yayu; Naid, Tadjuddin; Nuryanti, Siska
Journal Microbiology Science Vol 4, No 1 (2024)
Publisher : Fakultas Farmasi, Universitas Muslim Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56711/jms.v4i1.1006

Abstract

Tapak dara leaves (Catharanthus roseus L.) are known to be efficacious for relieving muscle pain, antidepressants, as well as medicine for various diseases such as relieving swelling from wasp stings, nosebleeds, and sore throats, antidotes, antibacterials, and lowering blood pressure in humans because they contain chemical compounds such as essential oils, phenolic acids, flavonoids, tannins, and alkaloids. This study aims to determine the antibacterial activity of the ethanol extract of tapak dara leaves against bacteria that cause skin infections. This type of research is done in an experimental laboratory using the agar diffusion method, which is based on inhibiting the growth of bacteria that cause skin infections. The stages of this research began with sample preparation, screening for antibacterial extract activity, and testing the antibacterial extract activity at several concentrations against the test bacteria that cause skin infections. This study obtained the results that the ethanol extract of tapak dara leaves tested positive for inhibition of the growth of Staphyloccus aureus, Staphylococcus epidermidis, Pseudomonas aeroginosa, and Propionibacterium acne based on the extract screening results. The results of this study also showed that the ethanol extract of tapak dara leaves at a concentration of 40% had the greatest inhibition using the agar diffusion method based on the diameter of the inhibition zone, with a value of 8.37 mm (Propionibacterium acne); 8.47 mm (Pseudomonas aeruginosa); 9.19 mm (Staphylococcus epidermidis); and 11.68 mm (Staphylococcus aureus)
Molecular Identification of Endophytic Fungi Isolated from Bidara Root (Ziziphus mauritiana Lam.) Using Polymerase Chain Reaction (Pcr) Jamaluddin, Siti Nurlillah; Fitriana, Fitriana; Amirah, Sitti
Journal Microbiology Science Vol 4, No 1 (2024)
Publisher : Fakultas Farmasi, Universitas Muslim Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56711/jms.v4i1.994

Abstract

The roots of the bidara plant have the potential as antibacterial agents. The antibacterial compounds in the roots of the bidara plant originate from secondary metabolites produced by endophytic compounds known as endophytic fungi. The purpose of this research is to identify the types of endophytic fungi in bidara roots. The identification of microorganisms can be done through morphology or molecular methods. However, morphological identification alone is unable to depict the morphospecies to the phylogenetic level of a microorganism, thus molecular identification using the Polymerase Chain Reaction (PCR) method is required. This study utilized root isolates from the bidara plant with the sample code IFAZ-6, which, after sequencing, yielded a pair of base pairs measuring 558bp. The results of BLAST and phylogenetic analysis revealed that the IFAZ-6 isolate has a close relationship with the species Clonostachys rosea., which has been recorded in the database with a 100% identity level.
Antibacterial Activity of Tunicata Ethanol Extract Polycarpa Aurata gainst Escherichia Coli and Salmonella Typhi by TLC-Bioautography and Agar Diffusion Selviati, Ratmi; Herwin, Herwin; Nuryanti, Siska
Journal Microbiology Science Vol 4, No 1 (2024)
Publisher : Fakultas Farmasi, Universitas Muslim Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56711/jms.v4i1.1040

Abstract

Tunicata Polycarpa aurata contains chemical compounds in the form of peptides and alkaloids  that are cytotoxic and have the ability as antibacterial against several pathogenic bacteria. The aims of this study was to determine the antibacterial activity of the ethanol extract of Tunicata Polycarpa aurata using the TLC-bioautography and diffusion method. Tunicata Polycarpa aurata was extracted by maceration and then evaporated to obtain a thick extract. The results of the antibacterial screening test showed the active extract at a concentration of 0.1% against Escherichia coli and Salmonella typhi. The results of testing for antibacterial activity using the TLC-bioautography with using ethanol: ethyl acetate (1:4) eluent showed results of Salmonella typhi bacteria with an Rf value = 0.27 and Escherichia coli bacteria with an Rf value = 0.27. While the results of testing the antibacterial activity the using the agar diffusion method showed the largest inhibition zona diameter at a concentration of 4% for Escherichia coli bacteria with an inhibition zone diameter of 12.55 mm, and Salmonella typhi with an inhibition zone diameter of 12.3 mm. Phytochemical screening tests on the ethanol extract of Tunicata Polycarpa aurata revealed  the presence of flavonoids, tannins, steroids/terpenoids, alkaloids and saponins.
Antibacterial Activity Test of Ethanol Extract of Basil Leaves (Ocimum basilicum) Against Streptococcus mutans and Porphyromonas gingivalis Soamole, Nurul Fatimah; Nuryanti, Siska
Journal Microbiology Science Vol 4, No 1 (2024)
Publisher : Fakultas Farmasi, Universitas Muslim Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56711/jms.v4i1.1024

Abstract

Basil leaves (Ocimum basilicum) is a type of plant used traditionally that has antibacterial potential. Antibacterial compounds are compounds that can inhibit the growth of bacteria. This study aims to obtain ethanol extract of basil leaves (Ocimum basilicum) to determine the activity and diameter of the largest inhibition zone against Streptococcus mutans and Porphyromonas gingivalis bacteria. This research was started by preparing a thick extract of basil leaves which was obtained by extracting basil leaves by maceration. Antibacterial screening test with concentrations of 0.1% and 0.5% against Streptococcus mutans and Porphyromonas gingivalis. The method used was agar diffusion with 6 concentrations in each treatment group of ethanol extract of basil leaves with concentrations of 0.5%, 1%, 5%, 10%, 15% and 30%. The results of the screening test gave activity at a concentration of 0.5% against the two test bacteria. The results of the antibacterial activity test of the ethanol extract of basil leaves against the test bacteria obtained the largest diameter of the inhibition zone at a concentration of 30%. Based on research, the ethanol extract of basil leaves has activity as an antibacterial.
Potential of Endophyctic Fungi Isolates IFAZ-6 from Bidara Root (Ziziphus mauritiana Lam.) as Antibacterials Againts Digestive Tract Infection janna, Nur; Fitriana, Fitriana; Amirah, Sitti
Journal Microbiology Science Vol 4, No 1 (2024)
Publisher : Fakultas Farmasi, Universitas Muslim Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56711/jms.v4i1.995

Abstract

Bidara root (Ziziphus mauritiana Lam.) has activity as an antibacterial against digestive tract infections. This study aims to determine the antibacterial activity of isolate IFAZ-6 endophytic fungi of bidara root against bacteria that cause digestive tract infections using the TLC-Bioautography method. Purification was conducted on the active isolate IFAZ-6 to obtain a pure endophytic fungi isolate. Subsequently, macroscopic and microscopic observations were carried out. Then, fermentation was performed in MYB (Maltose Yeast Broth) medium for 21 days. Screening and evaporation were conducted to produce an extract. The obtained extract was subjected to TLC analysis using a chloroform: methanol eluent (7:1). The TLC-Bioautography test was conducted against Escherichia coli, Salmonella typhi, Shigella dysentriae, and Vibrio cholerae bacteria. The TLC identification results showed one spot with an Rf value of 0.80 for the IFAZ-6 isolate. The chromatogram profile obtained from the TLC-Bioautography method for the IFAZ-6 isolate demonstrated antibacterial activity against bacteria that cause digestive tract infections.
Antibacterial Activity of Ethanol Extract of Sirih Cina Leaves (Peperomia pellucida) Using TLC-Bioautography and Agar Diffusion Methods Ahsan, Miftahul Khair; Herwin, Herwin; Rusli, Rusli
Journal Microbiology Science Vol 4, No 1 (2024)
Publisher : Fakultas Farmasi, Universitas Muslim Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56711/jms.v4i1.1058

Abstract

Sirih cina leaf (Peperomia pellucida) is a plant that contains active compounds of alkaloids, terpenoids, tannins, saponins, flavonoids and phenols that have potential antibacterial activity. This research aimed to determine the antibacterial activity of ethanol extract of sirih cina leaves (Peperomia pellucida) using the TLC-Bioautography and Agar Diffusion methods. Sirih cina leaf (Peperomia pellucida) was extracted by maceration and evaporated to obtain a thick extract. Screening test result showed that the extract was active at a concentration of 0.1% against the bacteria Propionibacterium acnes, Staphylococcus aureus and Pseudomonas aeruginosa. The results of antibacterial activity testing using the TLC-Bioautography method using the eluent methanol: chloroform (1:4) showed results with Rf values = 0.94, 0.80, 0.65, 0.54, 0.40, 0.30, 0.21, 0.10 against the bacteria Propionibacterium acnes, Staphylococcus aureus, and Pseudomonas aeruginosa. In determining the MIC, the value obtained was the same for the three test bacteria at a concentration of 0.5%, while the KBM value was at a concentration of 2% for Propionibacterium acnes bacteria, a concentration of 0.5% for Staphylococcus aureus bacteria, and a concentration of 1% for Pseudomonas aeruginosa bacteria. The results of antibacterial activity testing using the Agar Diffusion method obtained the largest inhibitory zone diameter at a concentration of 16% against Propionibacterium acnes bacteria with an inhibitory zone diameter of 23.20 mm, Staphylococcus aureus bacteria with an inhibitory zone diameter of 23.63 mm, and Pseudomonas aeruginosa bacteria with an inhibitory zone diameter. 24.48mm
Antibacterial Activity of Endophyte Fungi from Moringa Leaves (Moringa oleifera) in Gastrointestinal Infection Seminarwati, Sri; Nuryanti, Siska; Rusli, Rusli
Journal Microbiology Science Vol 4, No 1 (2024)
Publisher : Fakultas Farmasi, Universitas Muslim Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56711/jms.v4i1.1026

Abstract

Moringa leaves (Moringa oleifera) have potential as an antibacterial because they contain tannins, steroids, triterpenoids, flavonoids, saponins, alkaloids, where these compounds can damage bacterial cell membranes. This study aims to determine the isolates of leaf endophytic fungi (Moringa oleifera) which have antibacterial activity against digestive tract infection bacteria using the agar diffusion method. The results of the isolation of the endophytic fungi of Moringa leaves (Moringa oleifera) obtained 6 isolates which were continued with purification and macroscopic examination. The pure isolates obtained were subjected to screening tests on the test bacteria. The isolate with the IFDK-05 code as the most active isolate, were then fermented. The fermented products were then filtered to separate the supernatant and mycelia. This supernatant was then tested for antibacterial activity using agar diffusion. The results of testing the antibacterial activity of moringa leaf endophytic fungi isolate using the diffusion method in order to obtain the diameter of the inhibition zone for Escherichia coli bacteria (9,01 mm), Salmonella thypi (10,70 mm), Shigella disentryae (9,13 mm), and Vibrio cholerae (-10,95mm). Based on the results of this study, isolates of endophytic fungi of Moringa leaves (Moringa oleifera) have antibacterial activity
Antibacterial Activity of Endophytic Fungi Isolated from Bidara Roots (Ziziphus mauritiana Lam.) Against Bacteria That Cause Skin Infections Using TLC-Bioutography Sari, Andini Indah Permata; Fitriana, Fitriana; Amirah, Sitti
Journal Microbiology Science Vol 4, No 1 (2024)
Publisher : Fakultas Farmasi, Universitas Muslim Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56711/jms.v4i1.996

Abstract

One common disease found in tropical countries is skin infection. Inappropriate use of antibiotics to treat skin infections can lead to bacterial resistance, making it necessary to explore the source of bioactive compounds in natural materials. The Bidara plant (Ziziphus mauritiana Lam.) is known to have antibacterial properties. This research aimed to determine the antibacterial activity of endophytic fungal isolated from the parts of the Bidara plant against bacteria that cause skin infections using the TLC-Bioautography method. In this research, the isolate with the code IFAZ-6 was purified to obtain a pure endophytic fungal isolate, and macroscopic and microscopic examinations were conducted on that isolate. The obtained isolate was fermented for 21 days at room temperature using MYB (Maltose Yeast Broth) medium. The fermentation products were extracted using ethyl acetate (1:1). The obtained extract was further tested using TLC with a chloroform: methanol eluent (7:1). The TLC-Bioautography results showed Rf values of 0.81 and 0.58 indicating antibacterial activity against Staphylococcus aureus, Pseudomonas aeruginosa, Staphylococcus epidermidis, and Propionibacterium acnes bacteria. The identification of chemical components in the endophytic fungal isolate from the Bidara plant confirmed the presence of flavonoids, alkaloids, and anthraquinones. The extract from the endophytic fungal isolate with the code IFAZ-6 indicated antibacterial activity.
Free Antiradical Activity Test of Endophytic Fungi Isolates of Kasumba Turate (Carthamus tinctorius (L) Leaves from Galesong Takalar with KLT-Autography Method Sari, Dewi Mustika; Kosman, Rachmat; Rusli, Rusli
Journal Microbiology Science Vol 4, No 1 (2024)
Publisher : Fakultas Farmasi, Universitas Muslim Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56711/jms.v4i1.1027

Abstract

The kasumba turate plant (Carthamus tinctorius (L.) has the potential as a free antiradical. Free antiradical compounds in kasumba turate plants come from secondary metabolites produced by endophytic compounds known as endophytic fungi. Kasumba turate contains phenolic compounds and carotenoids that have antioxidant activity. This study aims to obtain isolates of endophytic fungi in kasumba turate (Carthamus tinctorius (L.) plants that can act as free antiradicals by KLT-Autography method. Five isolates IFDK 01, IFDK 02, IFDK 03, IFDK 04, and IFDK 05 were obtained. After testing the antioxidant activity of endophytic fungal isolates, two isolates were obtained that gave the best antioxidant activity, namely isolates IFDK 03 and IFDK 04. The results of KLT-Autography testing by spraying DPPH isolates IFDK 03 and IFDK 04 have the potential as free antiradicals showing yellow spots on a purple background having an Rf1 value, IFDK 03 isolate 0.94 and Rf2 0.55 and Rf1 value, IFDK 04 isolate 0.76 and Rf2 0.43

Page 4 of 8 | Total Record : 75

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