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Manilkara: Journal of Bioscience
Published by Universitas Terbuka
ISSN : -     EISSN : 29640164     DOI : https://doi.org/10.33830/manilkara
Core Subject : Health, Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology Neuroscience Veterinary
MANILKARA: Journal of Bioscience is designed to share research articles from academics, researchers, practitioners, and students on research in various aspects of biology and its applications. Papers for publication in this journal are selected through proper peer-review to ensure quality, originality, suitability, and legibility. This journal covers various disciplines in biology (microbiology, genetics, biochemistry, physiology, ecology, taxonomy, structure and development of animals/plants, and evolution); Biotechnology; and applied science in solving problems in the fields of biology (biomedical, agricultural, industrial, ethnobiological, and environmental). The journal is published biannually on February and August.
Arjuna Subject : Umum - Umum
Articles 4 Documents
 1 
Search results for , issue "Vol 3 No 1 (2024): Agustus" : 4 Documents clear
Analisis Karakteristik Biokimia serta Pertumbuhan Spirulina platensis pada Campuran Limbah Cair Keju dan Media Zarrouk’s dengan Konsentrasi Berbeda Apriastini, Marsiti; Agustini, Ni Wayan Sri
MANILKARA: Journal of Bioscience Vol 3 No 1 (2024): Agustus
Publisher : LPPM Universitas Terbuka

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33830/manilkara.v3i1.9664.2024

Abstract

Spirulina platensis is a filamentous, photosynthetic, multicellular blue-green microalga that thrives in various ecosystems. In addition to using Zarrouk's medium for the cultivation of S. platensis, wastewater can also serve as an alternative medium, as it contains nutrients such as nitrogen, phosphorus, and essential micronutrients for microalgal growth. The growth conditions of S. platensis are influenced by light, oxygen, and the pH of the medium. The aim of this study was to observe the growth and nutrient content of Spirulina platensis when cultivated in alternative growth media containing cheese liquid waste. This study utilized 15% cheese liquid waste in each treatment. The research involved several stages, including the cultivation of S. platensis to determine the growth pattern by measuring optical density (OD), protein content analysis using the Biuret method, and carbohydrate content analysis using the phenol-sulfuric acid method. The results showed that the lag phase of S. platensis growth occurred on day 2, during which the cells were still adapting to the wastewater. The exponential phase was observed on day 4, characterized by rapid and significant cell growth and development. In this phase, nutrients were sufficient to meet the needs of S. platensis cells, supported by favorable environmental factors. The highest protein and phycocyanin contents were observed in the mixture of cheese liquid waste and Zarrouk's medium at 100% concentration, while the highest carbohydrate and fat contents were observed in the mixture of cheese liquid waste and Zarrouk's medium at 50% concentration. In conclusion, the growth of S. platensis was most optimal in the mixture of cheese liquid waste and Zarrouk's medium at a 50% concentration.
Perbandingan Morfometrik Sistem Pencernaan Ikan Lele (Clarias batrachus), Ikan Nila (Oreochromis niloticus), dan Ikan Kembung (Rastrelliger sp.) Malini, Desak Made; Putri, Deviana Aulia; Setiawati, Tia
MANILKARA: Journal of Bioscience Vol 3 No 1 (2024): Agustus
Publisher : LPPM Universitas Terbuka

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33830/manilkara.v3i1.9811.2024

Abstract

Fish have a diversity of habitats and the types of food they consume, resulting in differences between species, especially in the morphology and morphometrics of their internal organs. One part of the fish that has significant differences is the digestive system. These differences allow fish to optimize their digestion to suit environmental conditions and the type of food they consume, thus ensuring better survival and reproduction. The aim of the study was to compare morphometrics in the digestive system organs of catfish (Clarias batrachus), tilapia (Oreochromis niloticus), and mackerel (Rastrelliger sp.). The research stages included sampling, anatomical preparation of fish, measurement of weight and length of digestive organs, measurement of Gastro Somatic Index (GaSI), measurement of Intestine Somatic Index (ISI), measurement of Gut Lengths (RGL), measurement of Relative Intestine Lengths (RIL), and measurement of Hepatosomatic Index (HSI). Analysis used one-way variance (oneway Anova) utilizing Statistical Package for the Social Sciences (SPSS) 22 software and Duncan's multiple range test. The results showed that GaSI, RGL, RIL, and HSI values of the three fish species were significantly different, while ISI values were not significantly different.  It was concluded that there were morphometric differences in the digestive system organs of tilapia, mackerel, and catfish.
Analisis Kemampuan Produksi Auksin dari Bakteri Endofit dan Plant Growth Promoting Rhizobacteria (PGPR) dalam Akar Cabai Merah Keriting (Capsicum annum L.) Maulida, Nurdalila; Rahmawati, Arrum; Dewi, Tirta Kumala; Simarmata, Rumella; Widowati, Tiwit; Kartika, Titik; Guswenrivo, Ikhsan; Nugroho, Deddy Triyono; Supriatna, Ateng
MANILKARA: Journal of Bioscience Vol 3 No 1 (2024): Agustus
Publisher : LPPM Universitas Terbuka

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33830/manilkara.v3i1.9817.2024

Abstract

Endophytic bacteria found in the roots, stems, and leaves of plants have potential as biocontrol agents and drivers of plant development, besides that in plant roots there are also plant growth promoting rhizobacteria (PGPR) with the same ability. The consortium of the two bacteria will produce Indole 3 acetic acid (IAA) compounds which are then converted into auxins that are beneficial to plants. The research aims to determine the ability of endophytic bacteria and PGPR from the roots of curly red chili plants in producing auxin hormones. The research work procedure includes rejuvenation of bacterial isolates, macroscopic and microscopic characteristics, measuring auxin levels using HPLC (High Performance Liquid Chromatography). The results showed that the consortium of 6 bacteria (3 endophytes and 3 PGPR) produced an auxin concentration of 46.122 ppm, a consortium of endophytic bacteria of 24.201 ppm, a consortium of PGPR bacteria of 162.723 ppm, and finally single endophytic bacteria and single PGPR bacteria produced auxin of A 158.913 ppm; B 64.882 ppm; C 93.923 ppm; CB1 240.817 ppm; CB2 186.807 ppm; and CMBC 11.689 ppm, while for the control 0 ppm. In conclusion, the highest auxin concentration was obtained from the single result of PGPR bacteria CB1 240.817 ppm.
Pengaruh Ekstrak Etanol Daun Muda Sungkai (Peronema Canescens Jack) Terhadap Jumlah dan Morfologi Normal Spermatozoa Mencit Jantan (Mus Musculus) Ananda, Mira; Rusmiati, Rusmiati
MANILKARA: Journal of Bioscience Vol 3 No 1 (2024): Agustus
Publisher : LPPM Universitas Terbuka

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33830/manilkara.v3i1.9823.2024

Abstract

Peronema canescens Jack is considered by the Dayak Bakumpai Tribe, Central Kalimantan as an enhancer of male fertility. The plant contains bioactive compounds such as tannins, flavonoids, steroids, terpenoids, alkaloids, saponins, and phenols. These compounds have the potential to increase the hormone testosterone which plays a role in increasing the number of spermatozoa. This study aims to study and analyze the effect of Peronema canescens Jack young leaf extract. Against the increase in the number and normal morphology of spermatozoa in male mice. The study used a Complete Randomized Design (RAL) experimental design with 24 male mice, which was divided into 4 treatment groups with 6 replicates. The K1 group (control) was given Na-CMC 0.5%, while the K2, K3, and K4 groups were given Peronema canescens Jack young leaf extract. with a dose of 87.5 mg/kg BB, 175 mg/kg BB, 350 mg/kg BB, respectively. The extract is administered orally as much as 0.5 mL per day for 35 consecutive days. The observed parameters were the normal number and morphology of spermatozoa. Results Statistical analysis using Anova and DMRT tests showed that the administration of ethanol extract of young leaves Peronema canescens Jack. increased the normal morphological number of male mouse spermatozoa.

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