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SAINS MEDIKA : JURNAL KEDOKTERAN DAN KESEHATAN

sainsmedika
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Published by Universitas Islam Sultan Agung

ISSN : 20851545 EISSN : 2339093X DOI : -

Core Subject : Health,

Health Professions Medicine & Pharmacology

Sains Medika is journal of medicine and health, is a peer-reviewed and open access journal that focuses on promoting medical sciences generated from Biomedical Sciences, Public Health, Clinical Sciences, and Medical Education to integrate researches in all aspects of human health. This journal publishes original articles, reviews article, and also interesting case reports.

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Articles 7 Documents

Search results for , issue "Vol 11, No 2 (2020): December 2020" : 7 Documents clear

CMCE Propolis Extract Improve Caspase 3 Expressions of The Hepatocytes and IL-6 Levels in Rats Exposed with CCl4 Firdaus, Chalid; Chodidjah, Chodidjah; Nasihun, Taufiqurrachman
Sains Medika: Jurnal Kedokteran dan Kesehatan Vol 11, No 2 (2020): December 2020
Publisher : Faculty of Medicine, Universitas Islam Sultan Agung (UNISSULA), Indonesia

Introduction: Liver plays an important role in the metabolic processes possibly exposed to the toxic materials. One of those hepatotoxic substances is carbon tetrachloride. The propolis extract contains the balsamic active substances, CAPE compounds and flavonoids. Flavonoids can prevent from the apoptosis and reduce the inflammation.Objective: To validate propolis extractâ€™s (CMCE method) ability in improving caspase 3 expressions of hepatocytes and IL-6 levels in Wistar male rats induced with carbon tetrachloride.Methods: An experimental study with a post-test only control group design applied to the study. The research subjects were 28 male Wistar rats, divided into four groups. The negative control group (CN-G) was only injected with CCl4, while the experimental groups were administered with the CMCE propolis at the dosages of 3.6 mg/200g (CM3-G), 7.2 mg/200g (CM7-G), and 14.4 mg/200g (CM14-G). The CMCE propolis extract was administered for 14 days and on day 14, the CCl4 was then given. The caspase 3 expression of hepatocytes was measured using HE liver cell preparations, while the IL-6 levels were measured using ELISA method.Results: The results of Mann-Whitney U statistical analysis showed that the hepatocytesâ€™ caspase 3 expressions in CM14-G (2.77 Â± 0.531), CM7-G (3.14 Â± 0.378), and CM3-G (4.22 Â± 0.690) were lower than those in CN-G (5.43 Â± 0.535), p<0.05. Meanwhile, the Post Hoc LSD analysis Results showed that IL-6 levels in CM14-G (55.032 Â± 9.336), CM7-G (78.362 Â± 8.313), and CM3-G (114.975 Â± 10.359) were lower those in CN-G (180.301 Â± 5.428), p<0.05.Conclusion: the administration of propolis extract (CMCE method) improve the caspase 3 expressions of hepatocytes and Il-6 levels in Wistar male rats induced with carbon tetrachloride.

Purslane (Portulaca oleracea) Ethanol Extract decreases hs-CRP levels and Total Score of Renal Tubular Degeneration in Rat Model of Gentamicine-Induced Renal Damage Saptaningtyas, Ragil; Chasani, Shofa; Nasihun, Taufiqurrachman
Sains Medika: Jurnal Kedokteran dan Kesehatan Vol 11, No 2 (2020): December 2020
Publisher : Faculty of Medicine, Universitas Islam Sultan Agung (UNISSULA), Indonesia

Introduction: Injection of intraperitoneal gentamicin at a dose of 60 mg/kg BW intra-peritoneal in rats for 7 days has been shown to trigger renal tubular degeneration and increase in high sensitivity C-reactive protein (hs-CRP) level. The use of purslane (Portulaca oleracea) to reduce hs-CRP levels and total score of renal tubular degeneration has not been reported.Objective: to determine the effect of purslane ethanol extract on hs-CRP levels and renal tubular degeneration score in gentamicin induced-renal damage in rats.Methods: This was a study using posttest only control group design. Twentyfive male Wistar rats aged 8-12 weeks, weighed 150-200 grams were allocated into 5 groups: normal (Nor-G), without treatment; negative (Neg-G), gentamicin (60 mg/kg BW), intraperitoneally; P-200; P-300 ; P-400 (gentamicin plus purslane extract at a dose of 200, 300, and 400 respectively) for 7 days. The hs-CRP was evaluated using the ELISA method. Total score of renal tubular degeneration was evaluated using modified score of Sarjadi.Results: there was a significant difference in mean hsCRP level and total score of renal tubular degeneration between groups (p <0.05). Posthoc analysis showed that hsCRP level and total score group of P-200, P-300 and P-400 were significantly lower than those of Neg-G, p <0.05. Meanwhile, the levels of hsCRP and total score of renal tubular degeneration in P-200, P-300, P-400, and Nor-G groups were not significantly different (p> 0.05).Conclusion: the administration of purslane ethanol extract at doses of 200, 300, and 400 mg/kg BW for 7 days improve hs-CRP level and total score of tubular degeneration similar to normal.Â

Effect of Four Antioxidants Combination on Number of Sertoli and Leydig Cells, Sperm Quality and Caspase-3 Expression in Rat Exposed to Cigarette Smoke Yuniarifa, Conita; Wibowo, Joko Wahyu; Nasihun, Taufiqurrachman
Sains Medika: Jurnal Kedokteran dan Kesehatan Vol 11, No 2 (2020): December 2020
Publisher : Faculty of Medicine, Universitas Islam Sultan Agung (UNISSULA), Indonesia

Introduction: The administration of antioxidant combination has been shown to be more effective than that of individual antioxidant in male infertility.Objective: to determine the effect of different combination of antioxidants (vitamin/vit C, vit E, glutathione and zinc) on the number of Sertoli and Leydig cells, sperm quality and texpression of caspase-3 in rats exposed to cigarette smoke.Methods: This was an experimental study using a post test only control group design. Thirty six rats were randomly divided into 6 groups to receive one of the following combinations of antioxidants: vit C and E, glutathione, zinc (T4-G); vit C and E, glutathione (T3-G); vit C and vit E (T2-G); Glutathione, zinc (T1-G); control positive (CP-G) cigarette smoke exposed only, normal (CN-G). The dose of vit C, vit E, glutathion and zinc was 9 mg/day, 1.8 IU/day, 1.8 mg/day, 0.2 mg/day respectively. All groups were exposed to cigarette smoke (3 cigarettes/day for 21 days), except for the CN-G group. On day 22, sperm samples were taken. Testicular tissue was taken for measurements of sperm quality, sertoli and leydig cell.numbers.Results: There was a significant difference in mean number of Leydig cells, sperm motility, sperm count and caspase-3 between groups (p<0.05). Post hoc LSD showed that the administration of combination of four antioxidant agent increasing the number of Sertoli cells and sperm morphology. The results also showed that group treated with 4 antioxidants combination had the highest number of Leydig cells, sperm motility, sperm counts and the lowest expression of caspase-3.Conclusion:The combination of vitC, vit E, glutathione and zinc affects the number of Sertoli and Leydig cells, sperm quality, expression of caspase-3 in rats exposed to cigarette smoke .

The Effect of Antlion (Myrmeleon sp.) Extract Towards Histopathology Image of Pancreas in Diabetic Mice Widyatmaka, Muhammad Naufal; Ismail, Akhmad
Sains Medika: Jurnal Kedokteran dan Kesehatan Vol 11, No 2 (2020): December 2020
Publisher : Faculty of Medicine, Universitas Islam Sultan Agung (UNISSULA), Indonesia

Introduction: Some studies indicated that antlions (Myrmeleon sp.) is traditionally used by many people in Indonesia as a diabetes treatmenter. However. reseach has not been done to find the effect of antlion extract in pancreas, an important organ in diabetes mellitus pathophysiology. Objective: To prove the effect of antlion extract towards histopathological feature of pancreas in diabetic mice.Methods: In the post test only control group design, 25 Swiss mice were divided into five groups. Normal group (Nor-G), Negative control group (Neg-G), and the other 3 groups were intraperitoneally injected with Streptozotocine 200 mg/kg to induce diabetic state. In Neg-G had no antlion treatment, whereas in At-14, At-28, and At-56 were treated with antlion extract at the dose of 14, 28, and 56 mg/kg BW respectively for 24 days. Pancreas then collected and prepared to be examined under microscope with 400x magnification. The score of pancreatic islet injury was determined by assessing structural damage and inflammatory cells infiltration. Kruskal-Wallis and Mann-Whitney were adopted as statistical analysis.Results: Mann-Whitney analysis indicated that the score of pancreatic islets injury in At-56 was significant lower compared to that of Neg-G, p<0.05. The score of pancreatic islets injury in At-56 also significant lower compared to that of At-14 and At-28, p<0.05. In contrary, when compared to that of Nor-G, the pancreatic islets injury in At-56 was not signicantly lower, p>0.05.Conclusion: Antlion extract treatment at the dose of 56 mg/kg BW was capable of reducing pancreatic islet injury in diabetic mice induced by streptozotocine.

Senam Sehat Anak Indonesia Improve Peak Expiratory Flow Rate in Children with Obesity Ramadhania, Farah Alya; Asmara Putri, Rahmi Isma; Setiawati, Erna; Nency, Yetty Movieta
Sains Medika: Jurnal Kedokteran dan Kesehatan Vol 11, No 2 (2020): December 2020
Publisher : Faculty of Medicine, Universitas Islam Sultan Agung (UNISSULA), Indonesia

Introduction: Excessive fat accumulation in the obese child causes complex problems in the respiratory system. Respiratory system function can be improved by exercise. One exercise model that is suitable for children is Senam Sehat Anak Indonesia (SSAI). The quality of the respiratory function system is measured using Peak Expiratory Flow Rate (PEFR).Objective: to validate the PEFRâ€™s differences in obese children before and after performing SSAI.Methods: This research using one group pre-posttest experimental design with total sample of 20 obese children using purposive sampling method. The research instrument uses weight scale, height scale, and peak flow meter. SSAI was given to all subjects twice a week for six weeks. Paired T Test statistical analysis was used to examine the PEFRâ€™s differences before and after performing SSAI, considered significant if p<0.05.Results: The PEFRâ€™s mean of all subjects before performing SSAI was 258.25 Â± 39.51 Lâˆ•minute, while PEFRâ€™s mean after performing SSAI twice a week for 6 weeks was 282.50 Â± 43.03 Lâˆ•minute, (p=0,000).Conclusion: The PEFRâ€™s mean of obese children was increase significantly after performing SSAI twice a week for 6 weeks

Comparison between Insulin-like Growth Factor Binding Protein-1 Level and Bishop Score as Predictor of Successful Labor Induction in Full Term Pregnancy with Preeclampsia Setiyorini, Nurul; Cahyanti, Ratnasari Dwi
Sains Medika: Jurnal Kedokteran dan Kesehatan Vol 11, No 2 (2020): December 2020
Publisher : Faculty of Medicine, Universitas Islam Sultan Agung (UNISSULA), Indonesia

Introduction: preeclampsia/eclampsia contributed to 30% of maternal mortality in RSUP Dr. Kariadi. Pre-induction bishop score assessment is a standard for cervical maturity estimation. Other predictors currently being developed, including insulin-like growth factor binding protein-1 (IGFBP-1). However, there is no cut-off point for IGFBP-1 examination in full term pregnancy, especially with preeclampsia and its complications. Objective: to find IGFBP-1 levels and Bishop Score differences in preeclampsia women with induction labor.Methods: this prospective observational cohort study was conducted in preeclampsia women with gestational age â‰¥37 weeks planned labor induction at Obstetrics and Gynecology Ward RSUP Dr. Kariadi Semarang and networking hospital during study period (n=66). History taking, physical examination, proteinuria, cervical mucus IGFBP-1, and bishop score calculation was performed. Statistical analysis was performed with paired-t test, followed by ROC for cut-off value of successful induction prediction, and logistic regression to determine confounding variables effect on IGFBP-1 levels.Results: The average Bishop score of 66 subjects was 2.5Â±1.81 and 2.6Â±1.8 for women who deliver â‰¤24 labor and in labor for â‰¤12 hours, respectively. Although the bishop score was higher in the successful labor group, there was no significant difference between groups. Mean IGFBP-1 value was 8.29Â±5.033 mcg/L with median value of 10.8 mcg/L. Successful induction had significant higher IGFBP-1 levels with area-under-curve (AUC) 0.76 and 8.145 cutoff value (p=0.002, RR=5.1).Conclusion: IGFBP-1 level with cutoff point 8.145 can be used as predictor of successful labor induction in term pregnancy with preeclampsia.

The Administration of Topical Aloe vera Extract Reduce the Number of Sunburn Cells and Expression of Caspase-3 on Post UVB-light-exposure Epidermis Soetrisno, Kusumawati; Subchan, Prasetyowati; Hussana, Atina
Sains Medika: Jurnal Kedokteran dan Kesehatan Vol 11, No 2 (2020): December 2020
Publisher : Faculty of Medicine, Universitas Islam Sultan Agung (UNISSULA), Indonesia

Introduction: Ultraviolet B (UVB) radiation triggers the formation of free radicals that cause apoptosis and sunburn cells (SBC) formation. Aloe vera contains anti-inflammatory and antioxidant compounds that can potentially inhibit this process. Objective: to assess the effect of topical Aloe vera extract administration on the decrease of SBC number and Caspase-3 expression on the epidermis after UVB light exposure.Methods: In a post-test only group design study, a single dose of 3 doses of Erythema (DEM) was performed on two groups of 6 week old BALB/c female rats. Group A (control) did not receive any topical treatment, and group B (treatment) were smeared with 75% Aloe vera extract before irradiation. Each group was divided into 4 sub-groups based on post-exposure time of 6 hours (A1 and B1), 12 hours (A2 and B2), 24 hours (A3 and B3), and 48 hours (A4 and B4). The expression of caspase-3 was assessed by immunohistochemical staining while the SBC number was measured using a microscope. The expression of caspase-3 was analyzed using the Kruskal-Wallis and Mann-Whitney statistical tests, while the number of SBC was analyzed using the one-way ANOVA statistical test and the post-hoc LSD test.Results: The results of the Kruskal-Wallis analysis showed a significant difference in Caspase-3 expression between groups, p <0.05. The results of Mann-Whitney analysis showed a significant difference in Caspase-3 expression between the A1-A3 and B1-B3 subgroups (p <0.05). One-way ANOVA analysis showed a significant difference in the number of SBC between groups (p <0.05). Post-hoc LSD analysis showed significant differences in SBC counts between groups A and B across all subgroups, p <0.05.Conclusion: Topical application of Aloe Vera extract decreased Caspase-3 expression and the number of SBC in UVB light-exposed skin.

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