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Anneke Pesik
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Rumphius Pattimura Biological Journal
Published by Universitas Pattimura
ISSN : -     EISSN : 2684804X     DOI : https://doi.org/10.30598/rumphiusv5i1y2023
Core Subject : Science, Education, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Immunology & microbiology
Rumphius Pattimura Biological Journal membuka kesempatan untuk mempublikasikan tulisan ilmiah dalam bentuk artikel penelitian terbaru, short communication, maupun review terhadap artikel dari berbagai bidang biologi, dan cabang ilmunya seperti biodiversitas, mikrobiologi, ekologi, zoologi, botani, genetika, molekuler, bioteknologi, biosistematika, parasitologi, perilaku, pertanian, dan kehutanan. RPBJ diterbitkan oleh Jurusan Biologi û Fakultas MIPA, Universitas Pattimura. Dalam 1 tahun RPBJ memiliki 1 Volume dan 2 Issue. Dewan editor kami berasal dari berbagai rumpun ilmu biologi yang aktif dalam publikasi ilmiah skala Nasional dan Internasional, sehingga mampu melakukan proses review dengan adil dan profesional. Artikel yang diterima akan diterbitkan sesuai dengan waktu yang ditentukan yaitu di bulan Maret dan bulan September, dan akan tersedia gratis bagi pembaca
Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Pertanian dan Biologi (Lain-Lain)
Articles 6 Documents
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Search results for , issue "Vol 6 No 2 (2024): RUMPHIUS Pattimura Biological Journal" : 6 Documents clear
RELATIONSHIPS OF FERNS IN THE POLYPODIACEAE FAMILY IN THE BUKIT KUJAU AREA, TEMPUNAK DISTRICT, SINTANG REGENCY, BASED ON MORPHOLOGICAL CHARACTERISTICS Juniarty, Syffa; Rafdinal, Rafdinal; Ifadatin, Siti
RUMPHIUS Vol 6 No 2 (2024): RUMPHIUS Pattimura Biological Journal
Publisher : Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/rumphiusv6i2p062-068

Abstract

Ferns belonging to the Polypodiaceae family are widely distributed across various regions of the world, particularly in forested areas and regions characterized by high humidity levels. The analysis of relationships among ferns can be conducted based on their morphological characteristics. The aim of this study was to determine the relationships among ferns of the Polypodiaceae family in the Bukit Kujau area of Tempunak District, Sintang Regency, utilizing morphological traits. This research was conducted from October to December 2023, during which 35 samples were collected in the Bukit Kujau area through purposive sampling. The relationships were analyzed using the UPGMA method in the NTSys program. The study identified seven species of ferns from the Polypodiaceae family: Platycerium bifurcatum (Cav.), Dynaria querfolia (L.), Davallia denticulatum (Burm.f.), Phymatodes scolopendria (Burm.), Diplazium esculentum (Rezt.), Pyrrosia numularifolia (Sw.), and Drymoglossum piloselloides (Linn.). Based on the grouping analysis, the seven species of ferns exhibited a similarity coefficient ranging from 0.37 to 1.00. At a similarity coefficient of 0.47, the species were grouped into three clusters, each distinguished by morphological characteristics such as sorus shape, sorus type, and sorus color.
OPTIMIZATION OF PROTOPLAST ISOLATION FROM DIFFERENT TYPES OF SAMPLES Mose, Windi; Wael, Syahran
RUMPHIUS Vol 6 No 2 (2024): RUMPHIUS Pattimura Biological Journal
Publisher : Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/rumphiusv6i2p056-061

Abstract

Isolation of protoplasts in several plant species still requires optimization to produce protoplasts that are viable and can be used for further analysis. This research aims to optimize protoplast isolation protocols in several plants: Orchid Dendrobium macrocarpus, potato Solanum tuberosum, and microalgae Phaeodactylum tricornutum, Botryococcus braunii, and Spirulina sp. The results showed that in vitro explant selection had higher sample uniformity, and a combination of enzyme solutions could be used to increase the effectiveness of protoplast isolation on D. macrocarpus, S. tuberosum, and Spirulina sp. This study provides information about protoplast isolation techniques and testing their viability as an attempt for plant breeding through micropropagation.
Three Behaviors of Apis cerana Fabricius in Response to Supplementary Feed of Pulp and Paste of Tongka Langit Banana Hartanto, Muchmin; Lamerkabel, Jacobus Suruka Aifaman; Mustapeng, Andi Maryani A; Riupassa, Pieter Agusthinus
RUMPHIUS Vol 6 No 2 (2024): RUMPHIUS Pattimura Biological Journal
Publisher : Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/rumphiusv6i2p069-077

Abstract

Apis cerana Fabricius is a honeybee commonly cultivated in Indonesia to produce honey. Additional feed can acts as a controller of honeybee colonies to protect them from migrate, in uncertain local weather conditions. Providing additional feed based on ‘tongka langit’ banana, Musa troglodytarum L. which have a high β-carotene nutritional content influences the adaptation, eating, and resting behaviors of Apis cerana. This study describes and analyses those three behaviors of Apis cerana to the provision of the feed. The study was conducted for 10 days with the provision of three treatment feeds, namely K+ (sugar water), P1 (fresh banana pulp), and P2 (banana paste) on the first day, and data collection was carried out the next day with a total of 5 times data collection on the number of individuals. Data represents for three types of behavior parameters (adaptation, eating, and resting) will be subjected to a two-way analysis of variance and continued with Duncan's test. Of the three treatments, result was found that P2 has the largest number of individuals in adaptation behavior, while in eating behavior it showed that K+ (as control) has the largest. Treatment K+ and P2 were also not significantly different, after Duncan’ comparison test. The number of individuals of resting behavior from P2 treatment observed on ninth day slightly increased to that of K+ treatment. This is reflected that P2 has potential as additional natural feed for Apis cerana, with high β-carotene content, then might inclusively turn to the honey, even in low levels.
EVALUATION OF TEA HERBAL BASED INGREDIENTS CLOVE LEAVES (Syzygium aromaticum) OF MALUKU INDONESIA Letlora, Marsya Elsina; Baharuddin, Alifia Audy; Wael, Syahran; Jaber, Zaid Mohamad; Rehena, Johanis Fritzgal
RUMPHIUS Vol 6 No 2 (2024): RUMPHIUS Pattimura Biological Journal
Publisher : Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/rumphiusv6i2p078-083

Abstract

Clove (Syzygium aromaticum) is one of the famous ancient spices and was used before christ. This plant belongs to the Myrtaceae family which is believed to be able to treat a variety of diseases. Cloves grow in tropical areas and range in height from 10-20 meters and are native plants that originated in Maluku. Clove that can be processed into a drink such as one is to make herbal tea from clove leaves. The type of research used is the experimental type, which is a study conducted to determine the results of organoleptic tests and compunds on clove leaf herbal tea. This research was conducted from July 29 to August 9, 2024 at the Basic Biology laboratory of Pattimura University. The results of the study showed that clove leaf herbal tea has a unique color, aroma and taste and meets quality standards because it has been tested at the level of color, aroma and taste preference. In the color of clove leaf herbal tea, it can be seen that the one ( very like) consists of 7 panelists, the result (like) consists of 3 panelists. Aroma it can be seen that for those who answered (very liked) consisting of 5 panelists, the result of the score (like) consisted of 1 panelist and the result (moderately) consisted of 4 panelists. For the results of the taste score, it can be seen that (very liked) consisted of 1 panelist, the result of the score (liked) consisted of 4 panelists, and the result of the score (moderate) consisted of 4 panelists and for the result of the score (disliked) consisted of 1 panelist. The results of the study showed that clove leaf herbal tea has a unique color, aroma and taste and meets quality standards because it has been tested at the level of color, aroma and taste preference. In the color of clove leaf herbal tea, it can be seen that the one ( very like) consists of 7 panelists, the result (like) consists of 3 panelists. Aroma it can be seen that for those who answered (very liked) consisting of 5 panelists, the result of the score (like) consisted of 1 panelist and the result (moderately) consisted of 4 panelists. For the results of the taste score, it can be seen that (very liked) consisted of 1 panelist, the result of the score (liked) consisted of 4 panelists, and the result of the score (moderate) consisted of 4 panelists and for the result of the score (disliked) consisted of 1 panelist.
EFFECTIVENESS OF Sargassum sp EXTRACT IN REDUCE BLOOD SUGAR LEVELS AND ACCELERATE WOUND HEALING ON THE SKIN OF DIABETES MELLITUS MICE (Rattus novergicus) Kaihena, Martha; Umagapi, Muhammad Cesar; Kaliky, Arief Rahman Shabri; Tarangi, Filep Marfil; Ukratalo, Abdul Mahid
RUMPHIUS Vol 6 No 2 (2024): RUMPHIUS Pattimura Biological Journal
Publisher : Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/rumphiusv6i2p093-100

Abstract

Diabetes Mellitus is a metabolic disorder characterised by elevated blood sugar levels beyond normal ranges, resulting from reduced secretion of the hormone insulin by pancreatic β cells and/or impaired insulin action. Diabetes therapy is often overseen by regulating blood glucose levels regularly and preventing or reducing the risk of complications. Sargassum sp is a variety of brown seaweed native to Indonesia with possible antioxidant and antidiabetic properties. This study seeks to evaluate the efficacy of Sargassum sp extract in lowering blood sugar levels and speeding up wound healing on the skin of diabetic mice. This is an experimental study that uses mice as experimental subjects. The mice were categorised into 5 groups (K-, K+, P1, P2, and P3). Their initial blood sugar levels were recorded, a skin incision was performed, and STZ was administered. When blood sugar levels rise, the K+ group receives metformin; on the other hand, P1, P2, and P3 are administered Sargassum sp extract at a specified dosage. The data were examined using ANOVA and SPSS. The analysis revealed that the water content of Sargassum sp was 4.32%, and the yield value of the concentrated extract was 8.75%. The ethanol extract of Sargassum sp has been revealed to lower blood sugar levels and speed up the wound healing process in mice with diabetes mellitus. The effects observed include decreased blood sugar levels and enhanced wound healing percentage, which are dose-dependent.
ANTIBACTERIAL ACTIVITY OF SEAGRASS EXTRACT Enhalus acoroides WITH DIFFERENT SOLVENTS AGAINST Streptococcus mutans BACTERIA CAUSE DENTAL CARIES Firman, Firman; Rieuwpassa, Fredrik; Mailoa, Meigy Nelce
RUMPHIUS Vol 6 No 2 (2024): RUMPHIUS Pattimura Biological Journal
Publisher : Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/rumphiusv6i2p084-092

Abstract

Treatment of Streptococcus mutans infection is generally done using antibiotics or chemical mouthwash, but long-term use can cause bacterial resistance and side effects for health. Therefore, it is important to find alternative treatments that are safer and more effective, one of which is through the use of medicinal plants. One plant that has potential as a source of antibacterial compounds is seagrass (Enhalus acoroides), which has been recognized in various studies to contain bioactive compounds such as flavonoids, saponins, and tannins, which can function as antibacterial agents. The purpose of this study was to determine the antibacterial activity of E. acoides extracts extracted with different solvents against S. mutans bacteria. The design used in this study is a completely randomised design (CRD) model. Testing the inhibition zone of E. acoroides using piper disc diffusion method with different concentrations of 25%, 50%, 75% and 100% with three replicates. Inhibitory zone diameter data were analyzed used ANOVA significant test and differences between treatments, and test of Real Honest Differences (BNJ) was conducted. Methanol and ethyl acetate extracts of E. acoroides can inhibit the growth of S. mutans bacteria that cause dental caries, and ethyl acetate has a stronger effect than methanol at higher concentrations.

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