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INDONESIA
Jurnal Serambi Biologi
Published by Universitas Negeri Padang
ISSN : -     EISSN : 27222829     DOI : -
Core Subject : Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology
Artikel ilmiah yang dipublikasi adalah artikel dalam bidang biologi (biodiversitas, biosistematika, ekologi, fisiologi, genetika dan bioteknologi, biokimia) yang meliputi semua bentuk mahluk hidup mulai dari mikroba, fungi, tumbuhan, hewan, manusia dan virus
Arjuna Subject : Biokimia, Genetika dan Biologi Molekuler - Biologi Perkembangan
Articles 187 Documents
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Analysis of Genetic Variations in PHT1 Gene Sequences in Rice (Oryza sativa) NCBI Popset 240028097 Using In-Silico RFLP Oliv Nurul Kanaya Nurul Kanaya; Afifatul Achyar
Jurnal Serambi Biologi Vol. 8 No. 1 (2023): Jurnal Serambi Biologi
Publisher : Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Padang

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Abstract

Abstract Rice is a plant that has high economic value. According to FAO, Indonesia is the third largest rice consuming country in the world. However, rice will become stunted and its growth will be stunted if there is a lack of phosphorus. The PHT1 gene plays an important role in plant growth and development, because this gene plays a role in taking phosphate from the soil. The PHT1 gene is transcribed when there is drought, salt stress, and nutrition in plants. Genetic variation in a population will affect the survival of an individual. This study used the restriction enzyme ScaI. This study aims to analyze genetic variation in the PHT1 gene sequence in NCBI Popset 2400280979 rice using RFLP in silico. The results showed that there was genetic variation in the rice PHT1 gene sequence and two allele variations present in 24 rice gene sequences using the restriction enzyme ScaI.
Dermatoglyphic Analysis of Astigmatism Sufferers in Student Population of Faculty of Mathematics and Natural Sciences, Universitas Negeri Padang Nafisa Arini
Jurnal Serambi Biologi Vol. 8 No. 1 (2023): Jurnal Serambi Biologi
Publisher : Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Padang

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Abstract

Gangguan mata silinder atau astigmatisme adalah kondisi dimana penglihatan penderita kabur, kondisi ini juga dapat dialami bersamaan dengan rabun jauh atau rabun dekat. Penelitian terdahulu banyak memberikan gambaran mengenai analisis dermatoglifi dan hubungannya dengan beberapa penyakit genetik namun masih belum banyak literatur yang memberikan gambaran analisis dermatoglifi pada penderita astigmatisme. Penelitian ini bertujuan untuk melihat jenis pola sidik jari apa yang paling banyak dimiliki oleh penderita Astigmatisme. Jenis penelitian yang dilakukan adalah penelitian deskriptif dengan metode analisis sampel Cross-Sectional. Subjek penelitian adalah mahasiswa Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Negeri Padang yang memiliki mata normal dan mata silindris. Penelitian dilakukan dengan melakukan analisis pada sampel pola sidik jari. Hasil penelitian menunjukkan bahwa dari total 100 responden didapatkan 18 responden penderita astigmatisme yang memiliki pola sidik jari arch, sedangkan responden dengan mata normal hanya didapatkan 13 orang dengan pola sidik jari arch. Hasil tersebut menunjukkan kemungkinan adanya hubungan antara pola sidik jari arch dengan astigmatisme.
Effect of Antimicrobial Activity 0f Starfruit Leaf Extract (Averrhoa bilimbi L.) on the Growth of Staphylococcus aureus Bacteria in Vitro Efliani Efliani; Dwi Hilda Putri
Jurnal Serambi Biologi Vol. 8 No. 1 (2023): Jurnal Serambi Biologi
Publisher : Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Padang

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Abstract

This study aims to determine the effect of the antimicrobial activity of star fruit leaf extract on the growth of S.aureus bacteria. This study was conducted using the complete randomized design (RAL) method with 5 treatments and 3 repetitions. The treatment carried out is: the concentration of star fruit leaf extract is 10%, 20%, 30%, 40%, and 50%. The data obtained were analyzed using ANOVA with a confidence level of α= 0.05 and further tested with DNMRT. Antibacterial testing is carried out by the disc diffusion method to determine the formed inhibitory zone. The results showed the average diameter of the formation of the inhibitory zone at a concentration of 10% of 10.10 mm, 20% of 12.56 mm, 30% of 13.08 mm, 40% of 14.57 mm, and 50% of 15.97 mm. The optimum concentration of star fruit leaf extract in inhibiting the growth of S.aureus bacteria at a concentration of 50% is 15.97 mm. Key words: Staphylococcus aureus, antimicrobial, star fruit, inhibitory zone.
Compatibility Test of the Biculture Consortium of Thermophilic Bacteria from Mudiak Sapan Hot Fahra Fahra; Irdawati Irdawati
Jurnal Serambi Biologi Vol. 8 No. 1 (2023): Jurnal Serambi Biologi
Publisher : Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Padang

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Abstract

Thermophilic bacteria are bacteria that are resistant to hot temperatures and have several other advantages in producing industrial fields. Bacteria also exist in the form of a mixture (consortium) where the bacteria work together to produce enzymes. This study aims to examine the compatibility of thermophilic bacterial isolates. This research is descriptive in nature using the Spread Plate Method. The results showed that the thermophilic bacterial isolates tested (MS 9, MS 12, MS 18 and MS 17) and all their compatible partners were seen with no inhibition zones. Keywords : Biofuel, thermophilic bacteria, compatibility test.
The Analysis of The Level Of Water Pollution in Lake Tambau Nagari Kampung Batu Dalam Danau Kembar District Solok Regency Through a Histopathological Study of Fish Kidneys Keiko Kasy Billah Keiko Kasy Billah; Abdul Razak; Yuni Ahda; Yusni Atifah
Jurnal Serambi Biologi Vol. 8 No. 2 (2023): Jurnal Serambi Biologi
Publisher : Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Padang

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Abstract

Water pollution is an indicator of deviations that occur in the properties of water from normal conditions. Lake Tambau, which is located in Jorong Air Tawa Utara, Nagari Kampung Batu Dalam, Danau Kembar District, Solok Regency, is one of the sources of fisheries used by the community to cultivate fish. The decline in water quality in Lake Tambau can be seen through physical observations such as the lack of water clarity, which indicates pollution in the lake water. Changes in environmental conditions that occur in Lake Tambau will be closely related to changes in the structure of kidney function in fish. Therefore research on kidney histopathology in fish is important to do in order to analyze changes in the histological structure of fish kidneys and analyze the level of water pollution that occurs in Lake Tambau. From the results of histopathological analysis of fish kidneys that have been obtained in Lake Tambau, it was found that there was hypertrophy, congestion, and necrosis. The results of a simple water analysis show that Lake Tambau water has a cloudy color, the temperature is above the normal threshold, has a pH of 9, there is sediment, and the color changes to dark when mixed with tea water which can be concluded that Lake Tambau water is heavily polluted. Key words water pollution,histopatology of fish kidneys
Test of Antimicrobial Activity of Rimbang Leaf (Solanum torvum) Ethanol Extract on Escherichia coli and Candida albicans Aura Iga Maharani; Rahmatul Huda Asra; Aulia Yunita; Resti Desmayanti; Husnul Khatimah; Dwi Hilda Putri
Jurnal Serambi Biologi Vol. 8 No. 1 (2023): Jurnal Serambi Biologi
Publisher : Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Padang

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Abstract

Aims: One of the types of traditional medicinal plants is rimbang (Solanus frowned). Rimbang is often used as an alternative medicine by rural communities because it is useful as a medicine for coughs, wounds, fever, boils and scabs. Rimbang leaf extract is known to contain flavonoids and tannins (polyphenolic) which have antimicrobial properties. The purpose of this study was to prove rimbang leaf extract was able to inhibit growth E.coli and C.albicans. Methods: Rimbang leaf extraction was carried out by maceration for three days with 96% ethanol solvent. Then evaporate using rotaryevaporator until thick extract is formed. The antimicrobial activity test was carried out by the agar diffusion method using disc paper by measuring the diameter of the inhibition zone for bacterial growth. The test concentrations used varied from 50%, 25%, 12.5%, 6.25% and 3.125%. Result: The results showed that rimbang leaf extract had antimicrobial activity against E.coli and C.albicans Main Conclusion: The antimicrobial activity test with strong inhibition was found in E. coli with a concentration of 6.25% an inhibition zone diameter of 12.15 mm, moderate inhibition at a concentration of 3.125% with an inhibition zone diameter of 9.5 mm, and moderate inhibition on C. albicans concentration of 50% with an inhibition zone diameter
The Prediction of the Interaction Genistein and Daidzein Compounds on ESR2 Expression by Molecular Docking Quratul Akyuni; Dwi Hilda Putri; Yuni Ahda
Jurnal Serambi Biologi Vol. 8 No. 1 (2023): Jurnal Serambi Biologi
Publisher : Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Padang

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Abstract

Aims: Estrogen is known to have various roles in the body. ESR2 is an estrogen receptor expressing estrogen receptor beta (ERβ). ERβ is expressed in normal breast epithelial cells as well as in various other tissues such as the uterus, ovary, prostate and brain, and breast cancer cells. One plant that is thought to have the potential to increase ERβ expression is soybean (Glycine max). Soybean plants contain very important phytochemicals, so they are widely used in the food industry as supplements and included in drug formulations. Methods: This type of research is descriptive research conducted using in silico method through the molecular docking approach. The metabolites used were genistein and daidzein for the target protein ESR 2 (PDB ID: 1QKM). Result: The docking results showed that the binding affinity value of the metabolites genistein, daidzein, and native ligand was -9.7 kcal/mol. The RMSD value obtained from LigRMSD was 0.40 with very similar visualization between the ligand test before and after docking. Main Conclusion: The conclusion of this study is that the metabolites of genistein and daidzein are predicted to have the ability to increase ESR2 expression in the human body.
The Differences Erythrocyte and Hemoglobin Levels of Biology Students and Sports Students Universitas Negeri Padang Nurulliza Dwi Aridya; Elsa Yuniarti
Jurnal Serambi Biologi Vol. 8 No. 1 (2023): Jurnal Serambi Biologi
Publisher : Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Padang

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Abstract

Darah adalah jaringan berbentuk cair yang terdiri dari dua bagian, yaitu plasma darah dan korpuskuli. Korpuskuli terdiri dari eritrosit, leukosit dan trombosit. Eritrosit atau sel darah merah mengandung haemoglobin yaitu protein yang mengandung besi, berperan dalam transport oksigen dan karbondioksida didalam tubuh. Penelitian ini bertujuan mengetahui perbedaan kadar eritrosit dan haemoglobin mahasiswa biologi dengan olahraga Universitas Negeri Padang. Metode penelitian yaitu analisis deskriptif, melakukan pengambilan sampel darah dan diperiksa dengan Hematology analyzer, selanjutnya dilakukan analisis dengan uji-t. Hasil pemeriksaan hemoglobin diperoleh p<0,0003 dengan rata-rata 16,30g/dL pada mahasiswa biologi dan 15,08g/dL pada mahasiswa olahraga, sedangkan data pemeriksaan eritrosit diperoleh p<0,1158 dengan rata-rata 5,615 10 ˆ6/uL pada mahasiswa biologi dan 5,193 10 ˆ6/uL pada mahasiswa olahraga. Kesimpulan penelitian ini yaitu terdapat perbedaan nyata pada kadar hemoglobin dan tidak terdapat perbedaan nyata pada kadar eritrosit mahasiswa biologi dengan olahraga Kata kunci Darah, Eritrosit, Hemoglobin, Mahasiswa, Olahraga
The Differences Hematocrit and Platelet Levels of Biology Students and Sports Students Universitas Negeri Padang Anika Fadila Sari; Elsa Yuniarti
Jurnal Serambi Biologi Vol. 8 No. 1 (2023): Jurnal Serambi Biologi
Publisher : Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Padang

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Abstract

Blood is 90% of the fluids that make up the human body. Blood cells are divided into erythrocytes, leukocytes, and platelets. To determine blood levels in a person's body can be done by measuring the hematocrit level. The hematocrit value in the body can be influenced by external factors such as routine physical exercise while internal factors such as fulfilling a balanced nutritional intake. This study aims to determine differences in hematocrit and platelet levels of biology students and sports students. This research is a quantitative descriptive. Subjects in this study were individuals who met the inclusion and exclusion criteria. The samples used were 15 biology students and 15 sports students. Analysis of the data obtained from the hematology analyzer examination was then carried out using the RStudio program for further statistical tests, namely the t-test with α = 0.05 with a 95% confidence interval. Based on the results of data analysis on hematocrit levels of biology and sports students, it was found that p<0.0002. While the platelet levels of biology students with sports students obtained p<0.0097. So it can be concluded that there are significant differences in the hematocrit and platelet levels of biology students and sports students. Keywords: Blood, Hematocrit, Hematology, Platelets, t-test
Isolation and Identification of Bacteria from Weaver Ants (Oecophylla smaragdina) Livia Juniati
Jurnal Serambi Biologi Vol. 8 No. 1 (2023): Jurnal Serambi Biologi
Publisher : Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Padang

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Abstract

Purpose: Weaver ants (O.smaragdina) are the most frequently encountered ants in the surrounding environment and certainly have an important role, one of which is as a producer of antimicrobial compounds. Microbes are found naturally in mixed populations and to obtain pure cultures, microbial isolation can be carried out. This study aims to isolate bacteria from O.smaragdina from the head, body and tail. Methods: Bacterial isolation from O.smaragdina was carried out macroscopically and microscopically. Results: The results showed that macroscopically there were 6 bacterial isolates. Then microscopically the 6 isolates belong to the gram-positive bacteria. Also obtained 6 isolates with bacillus form.

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