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INDONESIA
Jurnal Serambi Biologi
Published by Universitas Negeri Padang
ISSN : -     EISSN : 27222829     DOI : -
Core Subject : Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology
Artikel ilmiah yang dipublikasi adalah artikel dalam bidang biologi (biodiversitas, biosistematika, ekologi, fisiologi, genetika dan bioteknologi, biokimia) yang meliputi semua bentuk mahluk hidup mulai dari mikroba, fungi, tumbuhan, hewan, manusia dan virus
Arjuna Subject : Biokimia, Genetika dan Biologi Molekuler - Biologi Perkembangan
Articles 187 Documents
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The Effect of Cat Whisker Leaf Extract (Orthosiphon aristatus) on Estrus Cycle Recovery of Mice (Mus musculus L.) Musaddad; Ramadhan Sumarmin
Jurnal Serambi Biologi Vol. 6 No. 2 (2021): Jurnal Serambi Biologi
Publisher : Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Padang

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Abstract

This study aims to determine the effect of Kumis Kucing (Orthosiphon aristatus) leaf extract on the restoration of the estrous cycle of mice (Mus musculus) which has been induced by extending the estrous cycle with angsana stem bark extract (Pterocarpus indicus Wild). This study used a vaginal smear method or vaginal smear which was observed at 10.00 a.m to determine the phase of the estrous cycle for 24 days. Mice were divided into 4 treatment groups with 6 repetitions. The treatments were T1 (control), T2 (0.035 mg / head / day), T3 (0.135 mg / head / day), and T4 (0.175 mg / head / day). The data analiyzed using Analysis of Variance (ANOVA) and the DNMRT at p<0.05. The results showed O. aritatus the length of the estrous cycle were at T1 5.26; T2 6.5; T3 5.74; and T4 4.54 days. At T1, T3, and T4 have found that the estrous cycle was normal. But, the T2 is still in an extended state. It can be concluded that O. aristatus leaf extract can restore the estrous cycle of mice.
Effect of Papaya Leaf Extract (Carica papaya L.) on Colony Diameter and Percentage of Growth Inhibition of Fusarium oxysporum Meriza Fatma; Moralita Chatri; Mades Fifendy; Dezi Handayani
Jurnal Serambi Biologi Vol. 6 No. 2 (2021): Jurnal Serambi Biologi
Publisher : Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Padang

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Abstract

This study aimed to determine the effect of leaf extract concentration of C. papaya on the diameter of colonies F. oxysporum and the percentage of inhibition of growth of F. oxysporum. This research was conducted from February to April 2021 at the Integrated Research Laboratory, Department of Biology, Faculty of Mathematics and Natural Sciences, Padang State University. This study was an experimental study consisting of 5 treatments and 3 replications with the administration ofleaf extract at a C. papaya concentration of 0% (control), 10%, 20%, 30% and 40%. The data obtained were analyzed by means of variance (ANOVA) with Duncan's New Multiple Range Test (further testDNMRT)and the percentage of inhibition was analyzed descriptively. The results showed that leaf extract C. papaya at concentrations of 20%, 30% and 40% significantly affected the diameter of thecolonies F. oxysporum. The smallest colony diameter was at a concentration of 40% was 5.35 cm and the largest colony at a concentration of 10% was 6.61 cm. While the smallest percentage of inhibition at a concentration of 10% was 5% and the largest percentage of inhibition is at a concentration of 40% was 23%.
Comparison of Fenol-Kloroform Method and Mini-Prep CTAB Method for Chili (Capsicum annum L.) Plant DNA Isolation Sri Okta Handayani; Dwi Hilda Putri
Jurnal Serambi Biologi Vol. 6 No. 2 (2021): Jurnal Serambi Biologi
Publisher : Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Padang

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Abstract

Chili (Capsicum annum L.) is the most popular horticultural commodity in Indonesia. This high level of interest must be accompanied by an increase in the quality and quantity of chili production. One way to increase the quality and quantity of chilies is through plant breeding. Genetic variation analysis is one of the success factors in plant breeding. DNA isolation is the first step in analyzing genetic variation. There are various methods of plant DNA isolation, but DNA isolation methods using the fenol-kloroform method and CTAB mini-prep have not been widely reported. In this study, a comparison of the DNA isolation method of chili plants was carried out with the least DNA acquisition and contamination. This study used accessions of Capsicum annum L. (Cibinong03). The results of the qualitative evaluation study on confirmation of DNA samples were that there were no DNA bands found in the 0.8% agaorasa gel electrophoresis results, this result was inversely proportional to the results of the CTAB mini-prep method which produced DNA band bands in chilli accessions.
Analysis of Composition and Structure of Riparian Vegetation In The Batang Arau River Flow Region, Padang City, West Sumatera Yuni Selfia; Vauzia
Jurnal Serambi Biologi Vol. 6 No. 2 (2021): Jurnal Serambi Biologi
Publisher : Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Padang

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Abstract

Abstract. In the watershed ecosystem, riparian vegetation is one component that plays a role in influencing the ability of the soil to absorb and maintain water quality. The Batang Arau watershed is the most polluted river in the city of Padang, due to changes in land use which cause damage to the vegetation ecosystem. So that the impact on the loss of composition, structure and ecological function. Based on this, it is necessary to conduct research to determine the composition and structure of riparian vegetation in the Batang Arau watershed. The research was conducted from 26 December 2020 - 2 February 2021, in the Batang Arau watershed which includes station 1 (factory area), Station 2 (plantation area), Station 3 (unspoiled area). The method used is Linear Systematic Sampling. The results showed that in the factory area there were 64 individuals with 21 species belonging to 12 families. In the field area, 80 individuals were found with 38 species in 27 families. Unspoiled areas obtained 89 individuals with 58 species belonging to 26 families. INP results show that the area is dominated by Homalanthus papulneus plants. Factory area diversity index (H') = 0.69. Field area (H') = 1.34. Unspoiled area (H') = 1.20.
Inventory of Medicinal Plants that have the Potential to Treat Diabetes Mellitus in Sijunjung District Mardiana Ikhwan; Syamsurizal; Des M.; Moralita Chatri
Jurnal Serambi Biologi Vol. 6 No. 2 (2021): Jurnal Serambi Biologi
Publisher : Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Padang

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Abstract

Diabetes mellitus is a condition in which the pancreas no longer produces insulin properly, and results in an increase in glucose in the blood. Treatment using synthetic drugs is expensive, therefore some people use plants as medicine. One of them is the people of Sijunjung District, in this area traditional medicine is used to treat diabetes mellitus. This research was conducted from April to June 2021 using direct enumeration and interviews. Data was collected by means of in-depth interviews with key informants, namely traditional healers in every village and people who suffer from diabetes mellitus. The results showed that 43 types of plants belonging to 32 families have the potential as drugs for diabetes mellitus. The families that are often used as diabetes mellitus drugs by the people of Sijunjung District are the Asteraceae and Lamiaceae families. The plant organ that is most often used as a medicine for diabetes mellitus is the leaf. The way of processing the plant organs is boiled, pounded, squeezed, roasted and dried. How to use plants there are eaten and drunk. Most of the status of medicinal plants used are cultivated plants.
Sperm Quality of Bull Limousin (Bos taurus) in the Area of Ibuh, Payakumbuh Muthia Oktavia; Ramadhan Sumarmin
Jurnal Serambi Biologi Vol. 6 No. 2 (2021): Jurnal Serambi Biologi
Publisher : Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Padang

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Abstract

Beef cattle are cows that are specially raised for fattening because of their fast growth system and good quality aof meat. Limousine are one of the cows that have superior genetic quality. The Ibuh community began to like limousine cows because the grew rapidly and could be developed in an artificial insemination program. This cow cement can be used to produce frozen semen and distributed to other areas. The purposes of this study to determine the quality of male limousine bull sperm in the Ibuh area, Payakumbuh. The method used collection and identification at 3 bulls aged 3-4 years. Ejaculate sperm was taken once a week for 4 months to each male. There are two data analysis in this research. First parametric data were analyzed using ANOVA, and the second non-parametric data were presented in tabular form. The results showed that the mean macroscopic and microscopic sperm quality was normal, so that for parametric data (volume, pH, motility and concentration) analyzed by ANOVA, Fcount <Ftable was obtained so that it was not significantly higher at the p <0.05. It was concluded that the quality of ejaculated sperm from the limousine bull have good. Quality.
Optimasi Kondisi Fermentasi Cendawan Endofit Andalas (Morus Macroura Miq.) Isolat CED 3 Untuk Menghasilkan Senyawa Antibakteri Siti Nurayni; Dezi Handayani
Jurnal Serambi Biologi Vol. 6 No. 2 (2021): Jurnal Serambi Biologi
Publisher : Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Padang

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Andalas plant is medicinal plants with high potential in producing antimicrobial compounds. The direct use of antibacterial compound from Andalas plants can destroy its existence. Endophytic fungi are known to be able produce the same bioactive compounds as their host. Production antibacterial compounds can be done through the fermentation. The optimum conditions fermentation influenced by factors medium, time, temperature and pH. This reseach aiming to determine the optimum time fermentation, the optimum pH fermentation medium and the optimum temperature fermentation the endophytic fungus isolate CED 3 isolated from the leaves Andalas plant (Morus macroura Miq.) in producing antibacterial compounds. Antibacterial activity test by disc diffusion method. This research is a descriptive, held on January 2021 to June 2021 in the research laboratory of the Biology Department, FMIPA UNP. The results showed the optimum fermentation time for endophytic fungi isolate CED 3 was on day 6 against S. aureus with an average inhibition zone of 9.6 mm and 9,4 against E. coli. Optimizing pH optimum medium on pH 6 against S. aureus and pH 7 against E.coli. Meanwhile, optimum temperature optimization of isolate CED 3 was at 28°C-30°C against S. aureus and E.coli optimum at 26°C.
Kadar MDA Hati Mencit (Mus musculus L.) Setelah Diinduksi Ektrak Kulit Batang Kayu Manis (Cinnamomum burmannii) Ratna Nila Sari; Yuni Ahda; Siska Alicia Farma
Jurnal Serambi Biologi Vol. 6 No. 2 (2021): Jurnal Serambi Biologi
Publisher : Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Padang

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Abstract

Penelitian ini bertujuan untuk mengetahui pengaruh pemberian ekstrak kulit Kayu Manis (Cinnamomum burmannii) terhadap kadar malondialdehyde (MDA) pada hepar mencit (Mus musculus L.). Penelitian ini merupakan penelitian true experimental pada 30 ekor mencit yang diberi perlakuan selama 4 minggu. Mencit dibagi menjadi 3 kelompok perlakuan. Satu kelompok sebagai kontrol dan dua kelompok sebagai kelompok perlakuan dengan ekstrak kulit kayu manis. Data dianalisis dengan uji one way ANOVA. Hasil penelitian menunjukan bahwa kadar MDA (p<0,05) dan ekstrak kayu manis berpengaruh signifikan terhadap penurunan kadar MDA. Kesimpulannya Cinnamomum burmanni berpengaruh menurunkan kadar MDA secara signifikan pada hati Mus musculus.
KUALITAS SPERMA PEJANTAN SAPI SIMMENTAL (Bos taurus L.) DI DAERAH IBUH, PAYAKUMBUH Nindi Maiyora; Ramadhan Sumarmin
Jurnal Serambi Biologi Vol. 6 No. 2 (2021): Jurnal Serambi Biologi
Publisher : Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Padang

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Abstract

Salah satu permasalahan peternakan adalah meningkatnya kebutuhan daging sapi yang di ikuti dengan pertambahan jumlah penduduk. Sehinga, pemerintah melakukan upaya meningkatkan efisiensi ternak dengan melakukan program Inseminasi Buatan. Salah satu faktor keberhasilan inseminasi buatan ini adalah kualitas semen. Penelitian ini bertujuan untuk mengetahui bagaimana kualitas semen pejantan sapi Simmental (Bos taurus L.) secara makroskopis (volume, warna, konsistensi, dan pH) dan secara mikroskopis (gerakan massa, motilitas dan konsentrasi) di daerah Ibuh, Payakumbuh. Metode yang digunakan dalam penelitian ini dengan cara koleksi dan identifikasi. Pejantan yang digunakan berumur 3-5 tahun. Penampungan semen dilakukan 1 x seminggu dalam rentang waktu 4 bulan (September, Oktober, November 2020 dan Januari 2021). Analisis data bersifat deskriptif kualitatif. Data pada penelitian ini ada dua yaitu data parametrik yang disajikan secara kualitatif dalam bentuk persentase sedangkan data non parametrik dianalisis menggunakan Uji lanjut DNMRT p<0,05. Hasil penelitian menunjukkan bahwa kualitas sperma secara makroskopis dan mikroskopis termasuk dalam kisaran normal untuk syarat produksi semen. Berdasarkan hasil data parametrik menunjukkan bahwa tidak terdapat perbedaan yang nyata pada (p<0,05) volume, pH dan motilitas. Namun, berbeda nyata pada p<0,05% untuk konsentrasi spermatozoa pejantan sapi Simmental (Bos taurus L.)
The Ability of Fluorescent Pseudomonad to Produce Indole Acetic Acid (IAA) Maysyarah Ardiana; Linda Advinda
Jurnal Serambi Biologi Vol. 7 No. 1 (2022): Jurnal Serambi Biologi
Publisher : Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Padang

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Fluorescent pseudomonads are a group of bacteria derived from the rhizosphere of plants. Fluorescent pseudomonads have potential as plant growth promoters capable of producing Indole Acetic Acid (IAA). This study aims to obtain fluorescent pseudomonad isolates that can produce IAA. The fluorescent pseudomonad isolates used were isolate Pf31 (derived from the rhizosphere of the longan plant), Pf32 (derived from the rhizosphere of the cassava plant), Pf33 (derived from the rhizosphere of the guava plant), Pf35 (derived from the rhizosphere of the galangal plant), Pf36 (derived from the rhizosphere of the guava plant). red ginger), Pf37 (derived from the rhizosphere of the Alocasia plant), Pf38 (derived from the rhizosphere of the celery plant), Pf39 (derived from the rhizosphere of the turmeric plant), and Pf40 (derived from the rhizosphere of the Oxalis plant). namely the detection of the ability of fluorescent pseudomonads to produce IAA. The results obtained showed that the fluorescent pseudomonads isolates Pf31, Pf32, Pf33, Pf35, Pf36, Pf37, Pf38, Pf39, and Pf40 had the ability to produce IAA with the highest concentration produced by isolate Pf36 which was 9.86 ppm and the lowest concentration was produced by isolate Pf38. ie 3.65 ppm.

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