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Contact Name
Rijal Satria
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rijalsatria@fmipa.unp.ac.id
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+6282284574790
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INDONESIA
Jurnal Serambi Biologi
ISSN : -     EISSN : 27222829     DOI : -
Artikel ilmiah yang dipublikasi adalah artikel dalam bidang biologi (biodiversitas, biosistematika, ekologi, fisiologi, genetika dan bioteknologi, biokimia) yang meliputi semua bentuk mahluk hidup mulai dari mikroba, fungi, tumbuhan, hewan, manusia dan virus
Articles 187 Documents
Isolation of Endophite Bacteria From The Stem Of Taxus (Taxus sumatrana) And Testing Its Potential As an Antimicrobial Compound Produce Nanda Salma Rihadatul Aisy
Jurnal Serambi Biologi Vol. 8 No. 1 (2023): Jurnal Serambi Biologi
Publisher : Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Padang

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Abstract

Aims: Indonesia as a country that has an abundance of flora. Most of the flora that grows is used as a medicinal plant. Types of drugs that are being developed are antimicrobial drugs. A plant that is currently being widely studied to produce antimicrobial compounds is Taxus sumatrana. Taxus plants have phenolic compounds, lignans, and flavonoids. One way to obtain these compounds is by isolating endophytic bacteria. The aim of this study was to isolate endophytic bacteria from T. sumatrana stems and test their potential as antimicrobial compounds. Methods: Taxus stems were surface sterilized using 1% and 2% hypochlorite. The sterilized tissues were grown in NA medium for 48 hours at room temperature. The growing bacteria were identified by macroscopic and microscopic methods. Antimicrobial activity test was carried out using the inoculation point diffusion method. Result: The results obtained were 2 isolates that could inhibit the growth of E. coli. There were 22 isolates of bacteria that were successfully isolated, 2 of which were BKB3 (2) and BBB1 (1) which could inhibit the growth of E. coli with a low inhibition zone.
The Effect of Micro Radiation on Microbial DNA Nurul Pratiwi; Cindy Pramila; Fira Safitri; Syifa Kamila Namidya; Dwi Hilda Putri
Jurnal Serambi Biologi Vol. 8 No. 1 (2023): Jurnal Serambi Biologi
Publisher : Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Padang

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Aim One of the important steps in molecular studies is DNA extraction. Many methods have been developed for bacterial DNA extraction, using either kits or boiling techniques. For the boiling technique, heating can be done using a water bath, heatblock, and microwave. Microwave is a tool that uses micro radiation rays. This study aims to determine the effect of microwave irradiation on bacterial DNA. Methods The isolates used in this study were B.J.T.A.2.1 isolates which were inoculated into NB medium. Microwave exposure was carried out for 0, 30, and 90 seconds. Cultures were isolated using the QIAamp DNA Mini Kit. The quality of DNA after exposure to the microwave was analyzed from the electrophoretic DNA bands of PCR RAPD products. Results Microwave exposure of bacteria causes changes to DNA. PCR RAPD reaction using isolated DNA from bacteria exposed to microwaves produces new bands on electrophoresis results. The longer the bacteria are exposed to the microwave, the brighter and thicker the new DNA banding pattern will be. Main conclusion Microwave exposure to bacterial culture affects the isolated DNA. The longer the culture is exposed to the microwave, the brighter and thicker the new DNA banding pattern will be.
Seed Biopriming Using Plant Growth Promoting Rhizobacteria (PGPR) Milka Saputri; Linda Advinda; Azwir Anhar; Violita; Moralita Chatri
Jurnal Serambi Biologi Vol. 8 No. 1 (2023): Jurnal Serambi Biologi
Publisher : Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Padang

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Rhizobacteria are bacteria found on the surface of plant roots and are able to increase plant growth known as Plant Growth Promoting Rhizobacteria (PGPR). Rhizobakteri can be used for plant germination by biopriming. Biopriming is hydration of seeds in a controlled manner using biological compounds. Several species of plant growth promoting rhizobacteria that can be used for biopriming are Pseudomonas fluorescens, P. putida and Bacillus subtilis. Biopriming is an alternative method for controlling seeds from soil-borne pathogens and can help farmers use microbes to apply the crops they want. From several research results, biopriming has been proven to increase plant productivity.
Formulation of Pseudomonad Fluorescent as Plant Growth Promoting Rhizobacteria Haqil Triyatdipa; Linda Advinda; Azwir Anhar; Dezi Handayani; Irdawati
Jurnal Serambi Biologi Vol. 8 No. 1 (2023): Jurnal Serambi Biologi
Publisher : Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Padang

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Plant growth promoting rhizobacteria, known as Plant Growth Promoting Rhizobacteria (PGPR), are bacteria found on the surface of plant roots and are beneficial microorganisms. Fluorescent pseudomonads are a group of bacteria belonging to PGPR and have the ability to dissolve phosphates, produce nitrogen, phosphorus, siderophores, and Indole Acetic Acid (IAA) to stimulate plant growth. PGPR must be immobilized in a certain carrier in the form of a formulation so that it is easy to use, store, commercialize, and apply to the field. Due to the increasingly expensive instant media, the development of media formulations continues to be carried out which can be organic or inorganic.
Inventarisasi Semut Arboreal (Hymenoptera: Formicidae) di Kawasan Air Terjun Sarasah Uwak Anggun Rafila .
Jurnal Serambi Biologi Vol. 8 No. 1 (2023): Jurnal Serambi Biologi
Publisher : Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Padang

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Arboreal ants are ants that have dominant activity in trees. Several abiotic and biotic factors in trees affect the species of ants found, such as nesting sites, food sources and insect symbionts. This study aims to inventory arboreal ant species in the Sarawak Uwak waterfall area. The research was conducted on November 27, 2022, using the Beating method for collecting insects. The results found 48 individual arboreal ants, which belong to 10 species and 8 genera. In the Camponotus and Polyrachis genera each found 2 species, while in the genera Myrmicaria, Technomyrmex, Acanthomyrmex, Colobsopsis, Odontomachus and Pheidole each found 1 species.
Potential Of Pseudomonas fluorescens As Plant Growth Promoting Bacteria Claudia Ayesha; Linda Advinda; Violita Violita; Dezi Handayani
Jurnal Serambi Biologi Vol. 8 No. 1 (2023): Jurnal Serambi Biologi
Publisher : Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Padang

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Abstract

Plant growth promoting bacteria or Plant Growth Promoting Rhizobacteria (PGPR) are soil bacteria found in the root area which function to increase plant growth. Most of them came from the Gram-negative group with the highest number of strains from the genus Pseudomonas. Pseudomonas fluorescens is able to produce IAA, siderophores and phosphate solubilizing compounds, fixes nitrogen and exhibits plant growth promoting activity. Therefore, this bacterium is very potential as a PGPR. This study aims to collect and analyze articles related to the potential of Pseudomonas fluorescens as a plant growth promoting bacterium. This type of research is research that uses internet sources from the Google Scholar database. The stages consist of identification, screening, eligibility and acceptance. Based on the articles collected, it was found that Pseudomoas fluorescens is one of the Plant Growth Promoting Rhizobacteria (PGPR). PGPR affects plant health and soil fertility by offering an excellent combination of properties that are useful in promoting plant growth, namely producing IAA and siderophores, dissolving phosphate, fixing nitrogen and exhibiting plant growth promoting activity.
Inventarisasi Jenis Capung (Odonata) SubOrdo Anisoptera Di Kawasan Cagar Alam Maninjau Sumatera Barat Fauzarah Putri Noviza noviza
Jurnal Serambi Biologi Vol. 8 No. 1 (2023): Jurnal Serambi Biologi
Publisher : Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Padang

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Capung merupakan salah satu jenis keanekaragaman hayati yang dimiliki oleh indonesia. Jenis capung yang ada di indonesia sekitar 700 spesies yakni 15% dari 5000 spesies yang ada di dunia. capung Anisoptera sayap belakang lebih lebar dibandingkan sayap depan Capung Anisoptera memiliki ukuran tubuh lebih gemuk dan capung tersebut dikenal memiliki kemampuan terbang dengan cepat. Penelitian ini bertujuan untuk, menginventarisasi spesies capung (Odonata) subordo Anisoptera di Kawasan Cagar Alam Maninjau. Penelitian dilakukan pada bulan Juli 2022, menggunakan metode fotografi. Total 56 individu, empat spesies, tiga genera, satu famili capung yang dikoleksi dari Kawasan Cagar Alam Maninjau (Diplacodes trivialis, Neurothemis fluctuans, Orthetrum sabina, Orthetrum Chrysis). Jenis yang paling banyak ditemukan adalah genus Orthetrum memiliki persebaran paling banyak di Cagar Alam Maninjau capung jenis ini memiliki pemilihan habitat yang cukup luas bahkan di habitat yang tercemar.
Synergism each Isolates of Thermophilic Bacteria SSA2, SSA3, SSA4, and SSAS6 in Xylanase Production Shafa Thalita Azzahra Azzahra
Jurnal Serambi Biologi Vol. 8 No. 2 (2023): Jurnal Serambi Biologi
Publisher : Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Padang

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Enzymes are proteins that act as catalysts to speed up chemical reactions by lowering the activation energy. Enzymes and microorganisms can be utilized in the industrial field. The advantages of the application of enzymes in the industrial field, namely the product becomes easily decomposed, requires less energy, and does not produce hazardous waste. One of the potential enzymes in the industrial field is xylanase. Xylanase enzymes can be produced by thermophilic bacteria. The advantage of thermophilic bacteria is that enzymes are more stable and can be stored longer. There is a consortium of bacteria naturally in nature. Consortium has a better ability than pure culture in utilizing the substrate as an energy source. Thermophilic bacteria from Sapan Sungai Aro isolates SSA 2, SSA 3, SSA 4 and SSAS 6 can produce xylanase enzymes. Prior to the consortium, a synergism test was needed to find out if all isolates were synergistic. Furthermore, the synergism test was carried out using the disk diffusion method. The results of the test did not reveal any inhibition zones around the disc paper, so that all SSA 2, SSA 3, SSA 4 and SSAS 6 isolates were synergistic and further tests could be carried out for consortium research.
Potential of Pseudomonad Fluoresen as Control of Plant Diseases Silviana Okwisan; Linda Advinda; Dezi Handayani; Dwi Hilda Putri; Irma Leilani Eka Putri
Jurnal Serambi Biologi Vol. 8 No. 1 (2023): Jurnal Serambi Biologi
Publisher : Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Padang

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The use of fertilizers and pesticides provides various side effects such as environmental pollution and higher production costs due to the high prices of fertilizers and pesticides. Therefore it is necessary to look for other alternatives to control plant diseases. One of the safe and environmentally friendly control measures is to use biological control agents. Fluorescent pseudomonads are antagonistic bacteria that are widely used as biological agents for fungi, pathogenic bacteria and viruses. Biological control using fluorescent pseudomonads has been studied for its effectiveness in suppressing plant diseases. This study aims to collect and analyze articles related to the potential of fluorescent pseudomonads in controlling plant diseases. This type of research is research that uses the literature review method and collects literature using internet sources from the Google Scholar database. The stages consist of identification, screening, eligibility and acceptance. Based on the articles collected, it was found that fluorescent pseudomonads are potential and effective in controlling various plant diseases.
Fluorescent Pseudomonad Association in Plant Rhizosphere Intan Febriani; Linda Advinda; Dezi Handayani; Siska Alicia Farma; Dwi Hilda Putri
Jurnal Serambi Biologi Vol. 8 No. 2 (2023): Jurnal Serambi Biologi
Publisher : Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Padang

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Plant growth promoting rhizobacteria or known as Plant Growth Promoting Rhizobacteria (PGPR) are bacteria found on the surface of plant roots and are beneficial microorganisms. The rhizosphere is an ideal area for the growth and development of soil microorganisms. This situation is supported by its function, namely as a provider of nutrients and also as a place for the growth and development of microorganisms. One of the bacteria found in the rhizosphere of plants is the fluorescent pseudomonad. Fluorescent pseudomonads are biological agents that can be isolated from the surface area of plant roots. This study aims to collect and analyze articles related to the association of fluorescent pseudomonads from plant rhizosphere. This type of research is research that uses the literature review method and literature collection using internet sources from the Google Scholar database. This stage consists of identification, screening, eligibility and acceptance. Based on the articles collected, it was found that the use of fluorescent pseudomonads as a biological control agent has been widely carried out because of their ability to produce antimicrobial compounds.

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