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Ahmad Shafwan S. Pulungan
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Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Negeri Medan Jl. Willem Iskandar Psr V Medan Estate, Sumatera Utara
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JBIO: jurnal biosains (the journal of biosciences)
Published by Universitas Negeri Medan
ISSN : 24431230     EISSN : 24606804     DOI : https://doi.org/10.24114/jbio.v6i1
Core Subject : Health, Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology
Jurnal Biosains (JBIO) features works of exceptional significance, originality, and relevance in all areas of biological science, from molecules to ecosystems, (ie genetic, microbiology, ecology, biosystematic, biostatistic) including works at the interface of other disciplines, such as chemistry, medicine,physic and mathematics. We also welcome data-driven meta-research articles that evaluate and aim to improve the standards of research in the life sciences and beyond. Our audience is the international scientific community as well as educators, policy makers, patient advocacy groups, and interested members of the public around the world.
Arjuna Subject : Biokimia, Genetika dan Biologi Molekuler - Biokimia, Genetika dan Biologi Molekuler (Lain-Lain)
Articles 5 Documents
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Search results for , issue "Vol. 11 No. 1 (2025): JURNAL BIOSAINS" : 5 Documents clear
Identification of Secondary Metabolites of Kirinyuh Leaves (Chromolaena odorata L.) as Antibacterial Using GS-MS grasella, martha; Sulistyarini Gultom, Endang
JURNAL BIOSAINS Vol. 11 No. 1 (2025): JURNAL BIOSAINS
Publisher : Universitas Negeri Medan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24114/jbio.v11i1.64589

Abstract

This study aims to determine the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) against pathogenic bacteria and to determine secondary metabolites in kirinyuh leaves (Chromolaena odorata) using the GC-MS (Gas Chromatography-Mass Spectroscopy) method. The test bacteria used were Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa. The extraction method was carried out by maceration using 96% ethanol solvent. The crude ethanol extract of kirinyuh leaves in this study used concentrations of 30%, 40%, 50%, 60% and 70%. Using a UV-VIS spectrophotometer, the most effective concentration in inhibiting and killing the growth of Escherichia coli, Staphylococcus aureus and Pseudomonas aeruginosa bacteria was a concentration of 70%, with an average OD (absorbance) value of -0.2029, -0.055, and -0.0523. Then secondary metabolite identification was carried out using GC-MS (Gas Chromatography-Mass Spectroscopy) ethanol-water (9:1). The test results showed that a concentration of 70% was the most effective in inhibiting and killing Escherichia coli, Staphylococcus aureus and Pseudomonas aeruginosa bacteria. The content of secondary metabolites in the ethanol extract of kirinyuh leaves contains 33 secondary metabolite compounds, 6 of which are dominant compounds as antibacterials.
Temperature Effects on Jembrana Superficial Unit (JSU) Recombinant Protein Expression in Escherichia coli BL21 Maelani, Imelda; Indriawati, Indriawati; Ismail, Yulia Sari; Mulyani, Riska; Syahfitri, Widya
JURNAL BIOSAINS Vol. 11 No. 1 (2025): JURNAL BIOSAINS
Publisher : Universitas Negeri Medan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24114/jbio.v11i1.64590

Abstract

The efficiency of expression of recombinant proteins in host cells depends on achieving proper protein folding, solubility, and biological activity. Critical environmental factors, including temperature, pH, nutrient availability, and inducer concentration, significantly influence protein yield, stability, and functionality. Optimizing these conditions is essential to minimize protein misfolding and aggregation, which can adversely affect expression efficiency and reduce bioactivity. This study aims to measure the effect of temperature on the growth of Escherichia coli BL21 harboring the recombinant JSU protein, derived from the ENV-SU gene of the Jembrana virus. A controlled laboratory experiment was conducted in which bacterial cultures were incubated at 25°C and 37°C under identical conditions, except for temperature. The results indicate a statistically significant difference in bacterial growth between the two temperature conditions (p = 0.08), suggesting that E. coli BL21 exhibits enhanced growth at 37°C compared to 25°C. The mean bacterial growth rate at 37°C was 1.756, whereas at 25°C, the average growth was 1.425, demonstrating that higher temperatures facilitate bacterial proliferation. However, due to the relatively small sample size, further validation with a larger dataset is required to confirm these findings. These findings highlight the critical role of temperature optimization in enhancing bacterial growth and improving recombinant protein production efficiency, emphasizing the need for further investigation with a larger dataset to validate the results. Keywords: recombinant protein; temperature; IPTG; Escherichia coli BL21; JSU construct.
Antibacterial Activity Of Nutmeg Seed (Myristica fragrans Houtt.) Methanol Extract Against The Growth Of Staphylococcus aureus and Eschericia coli Dewi, Resmila; Kurniaty, Rina; Marniza, Erda
JURNAL BIOSAINS Vol. 11 No. 1 (2025): JURNAL BIOSAINS
Publisher : Universitas Negeri Medan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24114/jbio.v11i1.64594

Abstract

Nutmeg seeds (Myristica fragran Houtt.) are natural materials containing active compounds with potential antibacterial properties. This study aims to determine the antibacterial activity of nutmeg seed methanol extract and the effective concentration for inhibiting the growth of S. aureus and E. coli, as well as to compare the inhibition zones produced against both bacteria. The nutmeg seed extract was obtained through maceration extraction using 96% methanol solvent. The antibacterial activity was tested using the disc diffusion method with three different concentrations of the extract: 20%, 30%, and 40%, along with chloramphenicol (positive control) and 10% DMSO (negative control). The inhibition zone data were analyzed using ANOVA with SPSS 26, followed by the DMRT test. Phytochemical testing revealed that the methanol extract of nutmeg seeds positively contains alkaloids, saponins, flavonoids, quinones, and triterpenoids. The results showed that the methanol extract of nutmeg seeds at concentrations of 20%, 30%, and 40% produced inhibition zones against S. aureus of 11.83 mm, 12.56 mm, and 15.06 mm, respectively, and against E. coli, the inhibition zones measured 11.83 mm, 12.56 mm, and 15.06 mm. The study concludes that the methanol extract of nutmeg seeds exhibits strong antibacterial activity against S. aureus and E. coli, with the most effective concentration being 40%. Additionally, the nutmeg seed methanol extract tended to have better antibacterial properties against E. coli than against S. aureus.
Antibacterial Activity Of Ethanol Extract And Methanol Extract Of Guava Leaves (Psidium guajava L.) Againts Staphylococcus aureus Kurniaty, Rina; Dewi , Resmila; Yusup, Muhilal
JURNAL BIOSAINS Vol. 11 No. 1 (2025): JURNAL BIOSAINS
Publisher : Universitas Negeri Medan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24114/jbio.v11i1.64595

Abstract

Guava leaves contain secondary metabolites, consisting of tannins, polyphenolates, flavonoids, monoterpenoids, alcolloids, quinones, and saponins. The main component of guava leaves is tannins, the amount of which reaches 9-12%. Tannins are antibacterial by precipitating proteins. The antimicrobial effect of tannins is through reactions with cell membranes, inactivation of the function of genetic material. alkaloids, flavonoids can inhibit the growth of Staphylococcus aureus. This research aims to find out the activities antibacterial ethanol and methanol extract of guava leaves against Staphylococcus aureus. This study uses the disc diffusion method. Using 5 treatment groups, namely positive control using chlorampenicol antibiotics and for positive control using DMSO. For concentrations of 5%, 10%, 15% use ethanol extract and methanol guava leaves. This study is an experimental study, testing antibacterial activity using Staphylococcus aureus bacteria. The data obtained was analyzed using the One Way ANOVA test and continued with the DMRT/Ducan test to see the difference in each treatment. The average results of the diameter of the inhibition zone for ethanol extracts were 10.47 mm (5%), 11.95 mm (10%), 16.94 mm (15%). For methanol extracts it is 9.7 mm (5%), 12.1 mm (10%), 19.7 mm (15%). One Way ANOVA analysis followed by DMRT/Ducan showed that both shallot extracts have antibacterial potential and do not have significant differences. The conclusion of this study is that guava leaf ethanol and methanol extracts with concentrations of 5%,10% and 15% have potential as antibacterial against Staphylococcus aureus.
Antibacterial Activity Test of Breadfulness Leaf Extract Plaster Preparation ( Artocarpus altilis ) In Vitro and In Vivo In The Treatment of Mice Cut Wounds Simorangkir, Delisma Marsauli
JURNAL BIOSAINS Vol. 11 No. 1 (2025): JURNAL BIOSAINS
Publisher : Universitas Negeri Medan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24114/jbio.v11i1.64636

Abstract

The skin is the largest organ in the human body. One of the problems that often occurs in the skin is wounds. Wounds are damage or loss of tissue in the body caused by a factor, one type of wound is a cut wound. Cuts occur due to friction with objects causing linear tears in the skin and underlying tissue. Cuts can cause infections due to bacteria. Breadfruit leaves (Artocarpus altilis) contain flavonoid compounds that can regenerate the skin by increasing collagen in the skin and have bactericidal properties. This study aims to determine the optimal concentration in the activity of breadfruit leaf extract formulated in the form of plasters against in vitro and in vivo tests in the treatment of cut wounds in mice. This study used the maceration method in making the extract using 96% ethanol solvent. Breadfruit leaf extract was formulated into a plaster preparation with various concentrations F1: EEDS 10%, F2: EEDS 15%, F3: EEDS 20%, and F4: EEDS 25%. The plaster preparation was evaluated to determine the physical properties and compounds of the wound dressing material by conducting thickness tests, humidity tests, pH tests, organoleptic tests, FTIR tests, activity tests of the preparation against in vitro tests and in vivo tests. The results showed that the concentration of EEDS 25% with a value of 24.35 against the in vitro test using Staphylococcus aureus bacteria with a Hansaplast plaster comparator with a value of 16.35. In the in vitro test in the treatment of mouse cuts, it was stated that the concentration of EEDS 25% with a healing day of 10.6 days was close to the healing day of the Hansaplast plaster comparator with a healing day of 9.3 days.

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