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Ahmad Shafwan S. Pulungan
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Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Negeri Medan Jl. Willem Iskandar Psr V Medan Estate, Sumatera Utara
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JBIO: jurnal biosains (the journal of biosciences)
Published by Universitas Negeri Medan
ISSN : 24431230     EISSN : 24606804     DOI : https://doi.org/10.24114/jbio.v6i1
Core Subject : Health, Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology
Jurnal Biosains (JBIO) features works of exceptional significance, originality, and relevance in all areas of biological science, from molecules to ecosystems, (ie genetic, microbiology, ecology, biosystematic, biostatistic) including works at the interface of other disciplines, such as chemistry, medicine,physic and mathematics. We also welcome data-driven meta-research articles that evaluate and aim to improve the standards of research in the life sciences and beyond. Our audience is the international scientific community as well as educators, policy makers, patient advocacy groups, and interested members of the public around the world.
Arjuna Subject : Biokimia, Genetika dan Biologi Molekuler - Biokimia, Genetika dan Biologi Molekuler (Lain-Lain)
Articles 5 Documents
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Search results for , issue "Vol. 11 No. 3 (2025): JURNAL BIOSAINS" : 5 Documents clear
Comparison Cytological Staining Results of Pleural Effusion with Delayed Time Without Alcohol Fixative and With Alcohol Fixative: PERBANDINGAN HASIL PEWARNAAN SITOLOGI EFUSI PLEURA DENGAN WAKTU TUNDA TANPA FIKSATIF ALKOHOL DAN DENGAN FIKSATIF ALKOHOL Anggraini, Fransiska; Yohani Mahtuti, Erni
JURNAL BIOSAINS Vol. 11 No. 3 (2025): JURNAL BIOSAINS
Publisher : Universitas Negeri Medan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24114/jbio.v11i3.68026

Abstract

The delay in the examination of pleural effusion fluid may lead to damage to the specimens. To minimize this damage, the addition of fixative solutions is often employed, one of which is 50% alcohol. Therefore, this study aims to determine the effectiveness of using 50% alcohol in fixing smears of pleural effusion fluid that are delayed in examination, as well as to assess the morphological quality of cells in effusion smears stored with and without fixative solutions. The research method is experimental, utilizing a pretest-posttest design. Sampling was conducted using accidental sampling, with specimens categorized into four treatment groups (immediate examination, immediate examination with 50% alcohol fixative, delayed examination without fixative, and delayed examination with 50% alcohol fixative). Smears were stained using the Diff-Quik method. The assessment results were analyzed descriptively and with a paired T-test. In the comparison between immediate without and delayed without, the T-test results showed a significant difference (p = 0.002, <0.05). Conversely, in the comparison between immediate and delayed with fixative, no significant difference was found (p = 0.707, >0.05). The comparison between immediate without and immediate with fixative showed a significant change (p = 0.005, <0.05), while the comparison between delayed without fixative and delayed with fixative was not significant (p = 0.775, >0.05). The alternative hypothesis was rejected based on the paired T-test, as no significant difference was found in the delayed samples compared to the samples that were delayed with the addition of 50% alcohol fixative.
The Effect Of Liquid Organic Fertilizer Application On The Growth Of Biograss (Pennisetum purpureum cv. Biograss) Muhammad, farhan; Media Agus Kurniawan
JURNAL BIOSAINS Vol. 11 No. 3 (2025): JURNAL BIOSAINS
Publisher : Universitas Negeri Medan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24114/jbio.v11i3.68113

Abstract

One of the superior varieties developed by the Agricultural Biotechnology and Genetic Resources Research and Development Center (BBPSI Biogen) is the Biograss Agrinak elephant grass. This grass was released as a superior forage crop variety in 2021 and has an impressive yield potential of 319 tons/hectare/year. This study aims to analyze the effect of liquid organic fertilizer application on the growth and productivity of Biograss elephant grass (Pennisetum purpureum cv. biograss) as livestock feed. The research method used a Completely Randomized Design (CRD) with a non-factorial arrangement consisting of 4 treatments and 5 replications. The parameters observed in this study included leaf width, leaf length, plant height, and fresh yield of odot grass. The results showed that treatment P3 resulted in the highest increases in leaf width (3.44 cm), leaf length (62.10 cm), plant height (90.19 cm), and fresh yield of odot grass (687.5 grams), with significant differences compared to other treatments. Application of liquid organic fertilizer at a dose of 15 ml per 1 liter of water gave the best results and has the potential to be applied on a larger scale.
Efectiveness Of Carica papaya Seeds Ethanol Extract With Metronidazole Against Porphyromonas gingivalis In Antibacterial And Antibiofilm Activity Thebez, Vanessa Angela; Soesanto, Sheila; Natassya, Priska
JURNAL BIOSAINS Vol. 11 No. 3 (2025): JURNAL BIOSAINS
Publisher : Universitas Negeri Medan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24114/jbio.v11i3.68420

Abstract

Introduction: Porphyromonas gingivalis promotes the growth of periodontal biofilm. Metronidazole is one of the adjunctive therapies for periodontitis. The combination of metronidazole with natural products such as Carica papaya seeds ethanolic extract, which has proven to have antibacterial effects is expected to reduce the side effects caused by metronidazole. Objective: To determine the antibacterial and antibiofilm effects of C. papaya seeds ethanolic extract combined with metronidazole against P. gingivalis. Methods: In vitro laboratory experiment using post-test only control group design. The sample used were 100% C. papaya seeds ethanolic extract, combination of 1000 µg/mL metronidazole with extract 3.125%, 6.25%, 12.5%, 25%, 50%, and 100%, 1000 µg/mL and 2000 µg/mL metronidazole as positive control, and distilled water as negative control. Antibacterial test was performed using plate count method and antibiofilm using microtiter plate biofilm assay method. Results: Carica papaya seeds ethanolic extract alone showed effective antibacterial and antibiofilm effects against P. gingivalis. The combination of 1000 µg/mL metronidazole with extract 6.25% to 100% showed an antibacterial effect equivalent to 2000 µg/mL metronidazole. The combination of 1000 µg/mL metronidazole with 100% extract showed antibiofilm effects in 1, 3, and 24 hours time incubation. The combination of extract and 1000 µg/mL metronidazole demonstrated the best antibiofilm effect during the biofilm maturation phase. Conclusion: The combination of 1000 µg/mL metronidazole with ethanol extract of C. papaya seeds exhibited antibacterial and antibiofilm effects against P. gingivalis.
Investigation of Melaleuca leucadendron L. from South Sumatra: Phytochemicals and IC50-Based Antioxidant Potential Ernia, Rima; Sitindaon, Rina SE; Antara, Nyoman Yudi
JURNAL BIOSAINS Vol. 11 No. 3 (2025): JURNAL BIOSAINS
Publisher : Universitas Negeri Medan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24114/jbio.v11i3.69899

Abstract

Melaleuca leucadendron L. (commonly known as Gelam wood) is a plant that thrives in the swampy regions of South Sumatra. The utilization of this plant has historically been limited to its wood, leaving the potential of its leaves and seeds largely unexplored. Therefore, this study aimed to identify the species of Gelam wood and investigate the phytochemical content and antioxidant potential of its leaves and seeds.The methodology involved methanol extraction of the plant material, followed by comprehensive phytochemical screening and an antioxidant activity assay using the DPPH method.The phytochemical analysis confirmed the presence of several key compounds, with the Gelam extracts testing positive for Alkaloids, Flavonoids, Steroids, Saponins, Tannins, and Triterpenoids. Furthermore, the extract from the leaves and seeds demonstrated potent antioxidant activity, evidenced by an IC50​ value of 47.56 ppm, which classifies it as a strong antioxidant (<50 ppm).This research highlights the significant therapeutic potential of Melaleuca leucadendron leaves and seeds, suggesting they are valuable sources of natural antioxidants
Total Plate Count (Tpc) Test And Contamination Of Coliform Escherichia Coli And Salmonella Sp. Bacteria In Cucumulawak Jamu In Traditional Markets In Medan City Adma, Tarisa
JURNAL BIOSAINS Vol. 11 No. 3 (2025): JURNAL BIOSAINS
Publisher : Universitas Negeri Medan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24114/jbio.v11i3.70036

Abstract

Jamu is a traditional Indonesian drink derived from plants that are efficacious for use in efforts to maintain health and cure diseases. However, because the manufacturing process is still simple, jamu is at high risk of contamination by microorganisms such as fungi and pathogenic bacteria such as Escherichia coli and Salmonella sp. This study aims to determine the presence of Escherichia coli and Salmonella sp. Coliform contamination in Javanese turmeric herbal medicine sold in traditional markets. This study is based on the simple process of making jamu using hands, unclean clothes from jamu makers, and unclean processing areas, this will increase the risk of contamination and result in jamu being contaminated by microorganisms that can endanger consumer health. Data collection techniques use testing and counting of bacterial colonies with the TPC (Total Plate Count) method and Escherichia coli bacteria with the MPN (Most Probable Number) method which consists of Presumptive Test, Confirmatory Test and Complementary Test. The results of the analysis showed that in the TPC (Total Plate Count) test, three of the five samples, namely Sp 1, Sp 3 and Sp 4, showed results that were suitable for consumption because the TPC (Total Plate Count) value did not exceed the maximum limit of BPOM 2019 and two of the five samples, namely Sp 2 and Sp 5, showed results that were not suitable for consumption because they exceeded the maximum limit of BPOM 2019.

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