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Proceedings of Annual International Conference Syiah Kuala University - Life Sciences & Engineering Chapter
Published by Universitas Syiah Kuala
ISSN : -     EISSN : -     DOI : -
Core Subject : Education, Engineering,
Engineering Other
Arjuna Subject : -
Articles 63 Documents
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Search results for , issue "Vol 3, No 1 (2013): Life Sciences" : 63 Documents clear
Utilization of viable bone marrow derivat stem cells through an adaption in low oxigen tension as an attempt to increase cellular transplantation efficacy for spermatogenesis process Erma Safitri; Suzanita Utama; Candra Bumi; Sri Wigati Mardi; . Mulyani; . Helen; . Purwati; R. Heru Prasetyo; Mas’ud Hariadi; Fedik Abdul Rantam
Proceedings of The Annual International Conference, Syiah Kuala University - Life Sciences & Engineering Chapter Vol 3, No 1 (2013): Life Sciences
Publisher : Syiah Kuala University

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Abstract

Cellular transplantation using stem cells provides very promising solutions in the regeneration and repairment of cells that have experienced degeneration where recovery through medical or surgical intervension is impossible. However, the very low viability of transplanted stem cells limits the transplantation efficacy. The aim of this research was to obtain viable bone marrow derived stem cells by an adaptation treatment in a low oxygen tensioned in vitro culture. Low oxygen tension adaptation was adjusted to the niche of the stem cells in vivo. In this study, in vitro culture of stem cells in 1% oxygen was compared to those of the conventional culture in 21 % oxygen.Results showed that under 1% oxygentension cell proliferation was slower with larger or rounded triangle shaped cells, and senescence or dead cells was low. Meanwhile under 21 % oxygen tension cell proliferation was two fold faster with flattened and slender cells, and senescence or dead cells was higher. In conclusion, conventional in vitro culture under 21% oxygen caused cell aging (senescence) and rapid cell death, therefore the transplanted cells were not viable.
Benthic mollusc assemblages in subtidal coastal waters of Penang National Park, Malaysia Alianie Mustaffa; Omar Ahmad; Vararin Vongpanich; Anita Talib; Khairun Yahya
Proceedings of The Annual International Conference, Syiah Kuala University - Life Sciences & Engineering Chapter Vol 3, No 1 (2013): Life Sciences
Publisher : Syiah Kuala University

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Abstract

Benthic molluscs were sampled bimonthly from October 2010 to September 2011. Four sampling sites were selected (Teluk Bahang, Teluk Aling, Teluk Ketapang and Pantai Acheh) based on difference human activities in surrounding coastal waters of Penang National Park. Sampling activities were done starting at 200 m from the subtidal shoreline until 1200 m offshore. A total of 432 benthos samples were collected using 6’ X 6’ Ponar grab. From this study, 25 species from 21 families and 25 genera were identified. Particle size analysis was done and revealed Penang National Park coastal waters is a muddy area with more than 80% of the sediment samples diagnosed as mud. Whereas, in Teluk Bahang and Teluk Aling, the starting transects (200 m to 400 m) were consisted mixture of particle size but the proportion of particle size gradually changed to mud towards the shore. Based on Pearson’s Correlation, it was found that Nuculanidae, Megastomia sp., and Timoclea sp. showed positive correlations with sandy particles (1000 ;m-250 ;m) with r values ranging 0.88 to 0.96 at p0.01. The density of benthic molluscs was found highest in Teluk Bahang followed by Teluk Aling, Teluk Ketapang and Pantai Acheh with overall mean value 613.6 molluscs/m2. Penang National Park coastal waters are all considered shallow as the depth never exceeding 10 m.
Self regulation of single mother having child with autism in Banda Aceh Sitti Wahyuni
Proceedings of The Annual International Conference, Syiah Kuala University - Life Sciences & Engineering Chapter Vol 3, No 1 (2013): Life Sciences
Publisher : Syiah Kuala University

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Abstract

Self-regulation is needed in every individual, especially the single mothers who have child with autism. Good self-regulation of the single mother who have child with autism will have an important impact in helping children with autism to growing well. The research objective is obtaining the dynamics of selfregulationin the single mothers who have child with autism in Banda Aceh, by used qualitative research methods to the two respondents of research. Collect data by interviewing the respondents for ± 1.5 months. Analyze the data using the analysis case of per-respondent in narrative description. The research showed each respondent had different self-regulation, it is caused by differences in the chronology of case that has happened, differences in feelings of each respondent to accept this case, and differences in interpretation by each respondent in this case.
The impact of differences planting date against morphological characters of some wheat genotype in Berastagi of Karo District Dafni Mawar Tarigan; . Rosmayati; Chairani Hanum; Lollie A.P. Putri
Proceedings of The Annual International Conference, Syiah Kuala University - Life Sciences & Engineering Chapter Vol 3, No 1 (2013): Life Sciences
Publisher : Syiah Kuala University

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Abstract

Optimal planting date is one of the important factors affecting the wheat crop. Improper planting date can lead to a drastic decrease in wheat yield. Therefore, information about the adaptation of wheat plants at planting date at a particular location will benefit farmers in determining how best to utilize the wheat plants in each production system through morphological characters. Then conducted research The Impact of Differences Planting date against Morphology Character of some Wheat Genotypes in Berastagi of Karo district to determine the most appropriate wheat genotypes grown in Berastagi at certain times growing season through morphological characters. This research was conducted at Berastagi with two planting date (planting date I = late February to early June 2012 and planting date II = late October 2012 to early February 2013), using a Randomized Block Design (RBD) non factorial using 12 wheat plant,namely 2 varieties (Selayar / K and Dewata / L) and 10 genotypes that OASIS / SKAUZ / / 4 * BCN (A); HP1744 (B); LAJ3302 / 2 * MO88 (C); RABE / 2 * MO88 (D), H-21 (E), G-21 (F), G-18 (G); MENEMEN (H); BASRIBEY (I); ALIBEY (J). Observational data were tested with analysis of variance and combined analysis.The observed morphological characters were plant height, number of spikelet spike-1, number of grainspike-1 and grain weight spike-1. The results of analysis of variance and combined analysis showed that all parameters were observed give significantly different results for each planting date.
The relationship between maternal anemia of a term pregnancy and neonatal asphyxia in Banda Aceh . Nurjannah; . Munawar; Arie Prasetyowati
Proceedings of The Annual International Conference, Syiah Kuala University - Life Sciences & Engineering Chapter Vol 3, No 1 (2013): Life Sciences
Publisher : Syiah Kuala University

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Abstract

Maternal anemia is a common problem in pregnancy which causes maternal and fetal oxygen transport disrupted. The disorder may lead to hypoxia of the fetus inside the womb resulting in neonatal asphyxia which is the main cause of neonatal mortality on a term pregnancy. The study aimed to investigatethe relationship between maternal anemia in a term pregnancy with occurrence of neonatal asphyxia Banda Aceh. This is an analytic study using cross sectional design. The study population was all a term pregnant women who gave birth in Zainoel Abidin Hospital and Mother Child Hospital, Banda Aceh. Data werecollected from medical records of mothers and babies delivered from January to February 2012. Samples were taken by purposive sampling with 91 samples. Maternal (gravidarum) anemia is defined as the condition in which level of Haemoglobin (Hb) 11gr/dl and neonatal asphyxia defined as Apgar score 7 at the first or the fifth minutes. The data analyzed using chi square test (==0.05). The results showed that mother who had anemia and having baby with neonatal asphyxia was 10 (20.8%). It also showed that mothers who suffered from maternal anemia are 4.47 times more likely to have baby with neonatal asphyxia (p value= 0,023). In conclusion, pregnant women with anemia have a higher risk to deliver baby with neonatal asphyxia than those of not anemia.
Utilization of thermophilic cellulolytic bacteria isolates and Trichoderma harzianum fungi on rice strow composting Dini Mufriah; Rini Sulistiani
Proceedings of The Annual International Conference, Syiah Kuala University - Life Sciences & Engineering Chapter Vol 3, No 1 (2013): Life Sciences
Publisher : Syiah Kuala University

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Abstract

The thermophilic cellulolytic bacteria are potential organisms for applications such as in decomposition of organic materials and enzyme technology. One potential organic materials which is very abundant in Indonesia is rice straw, which have not been optimally utilized. The problem is, the compostingprocess of straw in nature is used to take along time, so it needs to add effective decomposer microoroganisms which can adapt on compost termperature rise. The aims of this research were to isolated and selected thermophilic cellulolytic bacteria and Trichoderma harzianum and tested the isolates on rice straw composting. This research is executed in laboratory in faculty of agriculture, University of Alwashliyah Medan. Twenty thermophilic cellulolytic bacteria were isolated from several process composting of rice strawand corn waste at high temperature, from peat land and sidebu-debu hot spring, while Trichoderma harzianum fungi that used in this research was already got from previous research. Cellulolytic activity was determined by ability of bacteria and fungi to degrade carboxymethyl cellulose (CMC) substrate. Cellulolytic microorganisms was selected based on clear zone which form surrounding the colonies. Cellulase activity was measured by dinitrosalisilic acid (DNS) method. Results showed that there were 5 isolates (JG12, JG9,JG7 JR2, SD1) showing the best ability to degrade CMC. JG12 isolate had the higest diameter ratio of clear zone and enzyme activity ( 2,6 and 5,07 x 10-3 U/mL), while T. Harzianum had ratio of clear zone 1,22 and enzyme activity 4,02 x 10-3 U/mL).Then the selected bacteria and T. Harzianum were test on rice straw composting. This selection was used complete block Random Design Factorial, which consist of bacteria isolates as the first factor (S0=control, S1= Straw+JG7, S2=Straw+JR2, S3=Straw+JG9, S4=Straw+JG12, S5=Straw+SD1) and T. Harzianum as the second factor( T0=control, T1=Straw+T. harzianum). The result of this research are the highest decreasing of ratio C/N is caused by the treatment combination S4T1 which is consist of JG12 bacteria isolate that isolated from corn waste composting and Trichoderma harzianum (C/N = 17,16).
Growth and proline content of potato Solanum tuberosum L. in vitro candidate tolerant to drought of origin callus Bilter A. Sirait; Iovie P. Roselyna; . Charloq
Proceedings of The Annual International Conference, Syiah Kuala University - Life Sciences & Engineering Chapter Vol 3, No 1 (2013): Life Sciences
Publisher : Syiah Kuala University

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Abstract

Screening of candidate tolerant potato (Solanum tuberosum L.) to drought in vitro from callus was aimed to determine the growth and proline content of potato plants. This research was conducted at the Laboratory of Tissue Culture of Dinas Pertanian of North Sumatra Province, Jl Karya Jasa No 6 Gedung Johor Medan, in May 2012 until May 2013. The initial research was callus formation followed byorganogenesis. Then, was treated by PEG, 1000, 2000 and 3000 ppm. The result indicated that increased concentration of PEG decreased the amount of leaves and number of internodes. The higher the PEG concentration, the higher the proline content of potato in vitro.
Near-real-time biomonitoring of heavy metals using the xenoassay® system M.Y. Shukor; N.A. Masdor; M.I.E. Halmi; K Kamaruddin; M.A. Syed
Proceedings of The Annual International Conference, Syiah Kuala University - Life Sciences & Engineering Chapter Vol 3, No 1 (2013): Life Sciences
Publisher : Syiah Kuala University

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Abstract

Heavy metals have widespread industrial uses and have been found in increasing quantities as contaminants in all components of the biosphere. Water and sediment of rivers near industrial areas such as the Juru River in Penang and Langat River in Selangor are polluted with heavy metals. Thus, rapid and fast methods to detect the presence of heavy metals in the environment are necessary. Existing instrumental methods such as atomic absorption and emission spectrometry are very sensitive but the sole use of these instruments for heavy metal detection is extremely expensive, needs a skillful person to operate and is not amenable to near-real-time analysis. The best scenario for routine biomonitoring of heavy metals is the marriage between instrument- and bioassays. Currently, the USEPA has recognized whole cell-based bioassays such as as PolytoxTM and Microtox® for the detection of heavy metals. Unfortunately these cell-based assays cannot be used as real-time or near real-time assays in the field as they require bulky incubators. Near-real-time monitoring of heavy metals giving results in less than one hour is very useful in environmental CSI (Criminal Scene Investigation) or ECSI where temporal and spatial concentrations of heavy metals in running waters are a challenge to environmentalists to pinpoint heavy metals POS (point of source) for legal purposes. Enzyme-based inhibitive assays are simple, rapid and fast and could be developed for near real-time assays. We have developed an inhibitive assay system –Xenoassay® based on proteases for the assay of heavy metals. The system could detect the heavy metals mercury, cadmium, lead, copper, zinc and silver at the sub parts per million level. Field trial near-real-time assay capability shows promising results.
Asses humoral immunity of wistar strains rats (Rattus norvegicus L.) which given ethanol extractbuas buas (Premna pubescens Blume) leaves Martina Restuati; Restianna Eliawaty Simanjuntak
Proceedings of The Annual International Conference, Syiah Kuala University - Life Sciences & Engineering Chapter Vol 3, No 1 (2013): Life Sciences
Publisher : Syiah Kuala University

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Abstract

The aim of the research was to to assess the effect of ethanol extract Premna pubescens leaves on humoral immunity and the body weight of rats (Rattus norvegicus L.). The white rats use 24 tails and devided into 4 groups with each group replicates 6 times. There are A0 as control which give with aquadest, A1 give with ethanol extract of Premna pubescens leaves, A2 give with ethanol extract of Premna pubescens leaves and Sheep Red Blood Cells (SRBC), and A3 give with SRBC only. White Wistar rats aged 3 months with body weight 100-300 grams. Aquadest and ethanol extracts of Premna pubescens given orally every day and SRBC given by injections on the 8th and 15th day . The rats blood taken by decapitation on 31th day. Measurements antibody titer of the rat as humoral immunity used hemagglutination method using a V microplate 96. The results were analyzed statistically with ANOVA. The result showed that the antibody titer at A2 increased significantly , where t (65,78) t table (4,94). This suggests that the ethanol extract of Premna pubescens leaves influence the humoral imunnity of Wistar strains rats using SRBC, The result of body weight statistically, tcount 0.23 ttable 3.10. It means that ethanol extract of Premna pubescens leaves did not influence the body weigh.
Optimum allocation unit catching small pelagic fish in the water in Kei Island Southeast Maluku J. Tahapary; Bruri B. Tumiwa
Proceedings of The Annual International Conference, Syiah Kuala University - Life Sciences & Engineering Chapter Vol 3, No 1 (2013): Life Sciences
Publisher : Syiah Kuala University

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Abstract

Small pelagic fish is one of the fish are potential resources and high compared to other kinds of fish in Kei Island Southeast Maluku. The aim of this research is to determine the optimum allocation unit arrest in the utilization of small pelagic fish in the Kei Island waters. An instrument grap used for catching small pelagic fish in the waters Kei Island is a lift net, purse seine, drift gill net and encircling gill net. A method of collecting data covering data resources fish, fuel, perational cost labor, the number of units arrest, and tracing of references pertaining to an object research. Analysis of data using linear goal programming ( LGP ) to provide information is important in allocating a unit of catching small pelagic fishoptimally, and that is how allocation grap, optimal an instrument used how big achievement the purpose of which is willed according to the target, and just how much resources be used in achieving its goal. A kind of small pelagic fish widely distributed in Kei Island waters is Decapterus sp., Rastrelliger sp., Selaroides spp., Sardinela sp., Clupea s.p and Stolephorus sp. The result of this research is optimum allocation unit catching small pelagic fish in the Kei Island waters for the implementation of allocation technology usually in the utilization of resources fish

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