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Proceedings of Annual International Conference Syiah Kuala University - Life Sciences & Engineering Chapter
Published by Universitas Syiah Kuala
ISSN : -     EISSN : -     DOI : -
Core Subject : Education, Engineering,
Engineering Other
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Articles 402 Documents
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Antibodies titer of dogs immunized by anti-idiotypic vaccine detected by using enzyme linked immunosorbent assay Sayu Putu Yuni Paryati; Retno D. Soejoedono; Eka Noneng Nawangsih
Proceedings of The Annual International Conference, Syiah Kuala University - Life Sciences & Engineering Chapter Vol 2, No 1 (2012): Life Sciences
Publisher : Syiah Kuala University

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Abstract

Rabies control programs, including extensive vaccination with attenuated or inactivated vaccines. However, such vaccines are not without problems and can have detrimental effects. Indeed attenuated vaccines can revert to a more virulent form, and inactivated vaccines may produce serious side effects. These facts, have led to the creation of a new generation of vaccines: recombinant-DNA vaccines, synthetic peptide vaccines, and anti-idiotypic vaccines. The aim of this study is to study the result of anti-idiotypic immunization methods in dogs detected by using enzyme linked immunosorbent assay (ELISA). Anti-idiotype antibodies against rabies (Ab2) were isolated from chicken blood, separated by means of ammonium sulfate precipitation, then dialyzed using PBS pH 8.0 for 24 hours at 2 – 8 oC and purified using affinity chromatography column. Three groups of dogs were immunized, group I was immunized intramuscularly (i.m) with purified IgY, group II was immunizded oraly (p.o) with purified IgY and group III was immunized intramuscularly (i.m) with rabies viral vaccines.  The antibody response (Ab3) was detected using Agar Gel Precipitation Test (AGPT). The efficacy of Ab3 was detected using ELISA. By ELISA, the result of immunization indicated that the level of Ab3 titers of anti-idiotypic vaccine immunized dogs intramuscularly are more than 0.5 IU/ml (protective according to WHO standard), and significantly higher than oraly immunization, but it significantly lower than Ab3 titers of rabies viral vaccine immunized dogs. The conclusion of this study is intramuscularly immunization of anti-idiotypic antibodies can induce protective immune response against rabies virus, although its lower than antibodies titer of viral vaccine, it has a good prospect for vaccine development in controlling rabies
EFFECT OF BIOCHAR RESIDUE, COMPOST, AND UREA COMBINATION ON GROWTH AND YIELD OF MAIZE (ZEA MAYS L.) MOHD. CHALISZAR; . ZAITUN; E. NURAHMI
Proceedings of The Annual International Conference, Syiah Kuala University - Life Sciences & Engineering Chapter Vol 4, No 2 (2014): Life Sciences
Publisher : Syiah Kuala University

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Abstract

The objective of this study was to know the effect of biochar residue, compost and urea fertilization on growth and yield of maize.  The research was conducted at University Farm Ie Seum Research Station, Aceh Besar district, Aceh Province, Indonesia.  The experimental arranged in a randomized complate block non factorial design with four replications.  There were eight treatment combinations: P1 (without biochar residue + without compost + without urea), P2 (without biochar residue + without compost + urea), P3 (without biochar residue + compost + without urea), P4 (without biochar residue + compost + urea), P5 (biochar residue + without compost + without urea), P6 (biochar residue + without compost + urea), P7 (biochar residue + compost + without urea), P8 (biochar residue + compost + urea).  Based on the plant growth, biochar residue, compost, and urea fertilizer treatment did not significantly affect on plant height age of 30, 45 and 60 days after planting, leaf number aged 30, 45 and 60 days after planting, stem diameter ages 30, 45 and 60 days after planting. At the P7 treatment (biochar residue + compost + without urea) gave the best value but does not differ significantly with all treatments tested.  Based on plant yield, biochar residue, compost, and urea fertilizer treatment did not significantly affect on cornhusk ear length, cornhusk cob diameter, cornhusk cob weight, cob length without cornhusk, cob diameter without cornhusk, cob weight without cornhusk, and yield.  At P5 treatment (biochar residue + without compost + without urea) cornhusk cobs and P7 (biochar residue + compost + without urea) cornhusk cobs and without cornhusk provide the best value but does not differ significantly with all treatments tested. 
Detection QTLs for grain quality traits and yield in two BILs population of rice Maryam Hosseini; Saadolah Houshmand; Shahram Mohamadi; Alireza Tarang; Mahmod Khodambashi; Hossein Rahimsouroush
Proceedings of The Annual International Conference, Syiah Kuala University - Life Sciences & Engineering Chapter Vol 1, No 1 (2011): Life Sciences
Publisher : Syiah Kuala University

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Abstract

 Abstract. Rice, the second most important crop after wheat, has been cultivated in Iran. Marketing conditions and quality traits after yield are the most important factors to adopt of new varieties. Therefore in recent years Iranian scientists decided to training on breeding improved high yield varieties with good grain quality.Quantitative trait loci (QTLs) yield and  grain quality traits of rice (Oryza sativa L.) with additive, epistatic, and QTL × environment (QE) interaction effects were studied using a mixed-model-based composite interval mapping (MCIM) method and two populations of Back cross inbred lines (BILs) with a common maternal parent.In each of the populations two or three main effects of QTLs were detected for yield, amylose content, gel consistency and gelatinization temperature. Some identified QTL clusters suggested pleiotropic effects could control these traits. The phenotypic variation of AAE and AA QTLs effects were smaller than main QTLs. We conclude that the major genes and some minor and modifier genes controlled rice grain quality and yield components. The information obtained in this study have used to manipulating the QTLs for these traits by molecular marker-assisted selection and the best lines with excellent quality and high yield recognized
Antimicrobial Peptides: Their Role in Innate Immune System and Usage in Future Drug Development Tristia Rinanda
Proceedings of The Annual International Conference, Syiah Kuala University - Life Sciences & Engineering Chapter Vol 5, No 2 (2015): Life Sciences
Publisher : Syiah Kuala University

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Abstract

Antimicrobial peptides naturally play a role in the innate  immune system of any living organisms. These small  molecules are known as "ancient weapon" and also  recognized as natural antibiotics. Antimicrobial  peptides  have  distinct  characteristics  including  their  unique  structures  which  are strongly  correlated  to  their  antimicrobial  activities.  Recently  the  development  of  antimicrobial peptides as future antimicrobial agents has become a major issue in the pharmaceutical business. This  article  will  discuss  the  natural  role  of  antimicrobial  peptides  in  host  defence  system  and  the relationship of their structure with antimicrobial activity. This article will also discuss the challenge in developing antimicrobial peptides as future antimicrobial agents.
CFD analysis of efficiency and pressure drop in a gas-solid square cyclones separator NOVI SYLVIA; . YUNARD; . ELWINA; . WUSNAH; YAZID BINDAR
Proceedings of The Annual International Conference, Syiah Kuala University - Life Sciences & Engineering Chapter Vol 4, No 1 (2014): Engineering
Publisher : Syiah Kuala University

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Abstract

In this paper, two small cyclones with the same hydraulic diameter and volume, which one is square and the other one is round (Lapple cyclone), are numerically compared. A pre-processor software GAMBIT was employed to set up the configuration, discretisation, and boundary conditions of the cyclone. The characteristics of the cyclone being studied was 0.2 m in diameter, receiving a gas flow rate of 0.1 m3/s with a particle mass loading of 0.01 kg/s. A commercial CFD code FLUENT 6.2.16 was employed to simulate the flow field and particle dynamics in the cyclone. The Reynolds averaged Navier–Stokes equations with Reynolds Stress Turbulence Model (RSTM) are solved by use of the finite volume method based on the SIMPLE pressure correction algorithm in the computational domain. The Eulerian–Lagrangian computational procedure is used to predict particles tracking in the cyclones. The velocity fluctuations are simulated using the Discrete Random Walk (DRW).The results show that collection efficiency of square cyclone is the better with increasing flow rate than round cyclone. The pressure drop in square cyclone is higher than the pressure drop in small round one.
DNA Barcoding: the Molecular Detective Mohd N. Siti-Azizah
Proceedings of The Annual International Conference, Syiah Kuala University - Life Sciences & Engineering Chapter Vol 3, No 1 (2013): Life Sciences
Publisher : Syiah Kuala University

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Abstract

The existing taxonomic identification of most taxa has mainly been throughmorphological characters. However, there are limitations to relying primarily on morphology when attempting to identify species during various stages of their development or when examining fragmentary or processed remains. DNA barcoding is a diagnostic tool in which short DNA sequences are utilised for species identification. We describe here some of the works that have been conducted in our laboratory as well with collaborators on the utilisation of this technique
Physico-Chemical Properties of Patchouli Oils (Pogostemon cablin) Separated by Fractional Distillation Method Yuliani Aisyah; Sri Haryani Anwar
Proceedings of The Annual International Conference, Syiah Kuala University - Life Sciences & Engineering Chapter Vol 2, No 2 (2012): Engineering
Publisher : Syiah Kuala University

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Abstract

Patchouli (Pogostemon cablin) is one species of the vegetation that produces essential oil known as patchouli oil. The quantity of patchouli alcohol contained in patchouli oil determines the oil quality. This study investigates fractional distillation of patchouli oils (Pogostemon cablin) to increase the level of patchouli alcohol in patchouli oils. The separation process is carried out by fractional distillation method applying four fractionation temperatures: 120°C, 125°C, 130°C, and 135°C. The compositions of each fraction were identified using Gas Chromatography – Mass Spectrometry (GC-MS). Patchouli oil A and B with initial patchouli alcohol content of 27.03% and 36.87% were successfully fractionated resulting four fractions of patchouli oils. The final levels of patchouli alcohol are increased to become 35.35% and 43.62%, respectively as well as the densities of the four fractions
Meristematic shoot tip culture obtain good quality seedling of citrus (Citrus nobilis var. brastepu) free from citrus vein phloem degeneration Isnaini Nurwahyuni; Justin A. Napitupulu; . Rosmayati; Fauziah Harahap
Proceedings of The Annual International Conference, Syiah Kuala University - Life Sciences & Engineering Chapter Vol 2, No 1 (2012): Life Sciences
Publisher : Syiah Kuala University

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Abstract

Meristematic shoot tip culture to obtain good quality seedling of citrus (Citrus nobilis Var. Brastepu) free from citrus vein phloem degeneration (CVPD) is explained in this research. The method is conducted by using a diseased plant and is then propagated in optimum conditions. The research is conduted in Tissue Culture Laboratory Department of Biology, University of North Sumatra, Indonesia. Optimum conditions for citrus propagation have been obtained to produce good quality of Citrus seedlings that is free from CVPD. The analysis of the DNA using PCR method has confirmed that the seedlings are healthy and free from CVPD. Genomic analysis using 10 RAPD markers show the planlets regenerated from the cultures are 100% similar with the mother plant. 
Microstructure and Optimization of Sweet Potato-Rice Simulated Chips Adil Basuki Ahza; Arief Munandar; Subarna Suryatman
Proceedings of The Annual International Conference, Syiah Kuala University - Life Sciences & Engineering Chapter Vol 5, No 2 (2015): Life Sciences
Publisher : Syiah Kuala University

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Abstract

Optimization of formula, baking and frying condition, and microstructure of sweet potato - rice simulated chips were studied using a completely randomized factorial design based on its physical, chemical and sensorial characteristics. The formulas were enriched with a mixture of skim milk, carrots, celery and  temurui leaf powders to enhance its  functional properties. Parameters to measure chips quality include color, texture, moisture content, ash content, protein content, fat content, carbohydrate, and hedonic ratings of sensory analysis. The result showed that fried simulated chips tend to be more preferable compared to the baked simulated chips. Fried simulated chips were generally slightly darker than the baked chips. Both fried and baked simulated chips’ colors were dark yellow. The high temperature frying produced finer microstructure and massive gelatinization of the fried chips. The optimum formula and cooking process of simulated chip was obtained from sweet potato-rice simulated chips with ratio of 60:40 fried at 160°C for 1minute; and the sweet potato: rice with ratio of 80:20, baked at 180°C for 8 minutes. Best fried simulated chip color was dark yellow, with its brightness values(L) 46.77±0.00, yellowness value(a) +4.83±0.01, redness value(b) +34.40±0.06, hardness 827.77±43.78gf, moisture content 2.95 ± 0.28%(db), ash content 1.95±0.08%(db), protein content 3.07±0.13%(db), fat content 28.01±0.40%(db), and carbohydrate content 64.02±0.07%(db). Panelists tended to prefer the fried simulated chips with an overall score of 5.37±1.24c. While the best baked simulated chips color was dark yellow, with brightness values(L) 69.31±0.01, yellowness(a) +1.04±0.00, redness(b) +29.96±0.02, hardness  382.97±43.66gf,  moisture  content  2.37±0.76%(db),  ash  content  3.07±0.11%(db), protein    content    4.09±0.00%(db),    fat    content    1.67±0.25%(db)    and    carbohydrate 88.80±1.12%(db). Baked simulated chips tended to be neutrally acceptable with an overall rating of 4.34±1.35ab.
Preparations of chemical sensors for simple formalin detection in contaminated food (A model for final project works for pre-service teachers in chemistry subject) M. Adlim; . Hasan; Zarlaida Fitri; Yulida Amri; Martina Sari; Saiful Mahya
Proceedings of The Annual International Conference, Syiah Kuala University - Life Sciences & Engineering Chapter Vol 1, No 2 (2011): Engineering
Publisher : Syiah Kuala University

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Abstract

Teaching chemistry must include some learning skills in scientific works. The students must have experience conducting small research in chemistry to train them the scientific methods. Some chemistry projects have been carried out by the students of teaching training college (FKIP) as called pre-service teachers in chemistry subject.  The students were given research problem that is how to formulate a simple sensor for detection of formalin in contaminated food. The students were asked to review literature on the composition and analysis technique of formalin. The students wrote the research proposal and presented their proposal in front of four reviewers before they start conducting research. The project of formalin sensor preparation was started with studies of the active reagent composition in liquids phase, immobilizing the active reagent in soft media, and following with studies of the sensor sensitivity toward formalin contamination in food. The Schiff Fuchsine (SF) and NASH methods for aldehyde determination were chosen as the basic theory for formalin determination.  Schiff Fuchsine (SF) in diluted sulfuric acid solution was kept overnight before use.  The media for holding of the reagent was cotton coated with chitosan, then it was pasted in tacon foil, this sensor was called forpastrip (student project-1). It could detect formalin as low as 2% (v/v) of formalin or equivalent with 0,8% of formaldehyde in contaminated food and the expired date was 10 weeks. The forpastrips was tested on food and the chemical interference was also studied (student project-2). Another project was also carried by replacing the cotton with synthetic felt and this formula was recorded as student project-3. The SF reagent immobilized in synthetic felt coated by chitosan was inserted into narrow-plastic straws. The sensitivity of this sensor increased and the detection limit as low as 0.25% (v/v) of formalin in contaminated food and the expired date was more than 12 weeks. The forth project was replacing SF with NASH reagent immobilized in cotton coated by chitosan and inserted in narrow-plastic straws. The detection limit was 0.015% (v/v) of formalin in contaminated food. All Sensors were still effective to detect formalin in sample although the sample contained of sugar, salt, fat and protein as the impurity. The research findings were written by students in their final report (script, like a thesis) after approved by the two supervisors. They students then presented their research in comprehensive exam in front of reviewers before they were graduated. Having interviews with students, they claimed that they have experience in conducting research, have followed all scientific methods. They said the research theme is very interesting, simple, contextual and it might be applied in high schoo

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