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INDONESIA
Journal of Microbiology, Biotechnology and Conservation
Published by Universitas Mataram
ISSN : -     EISSN : 3090708X     DOI : https://doi.org/10.29303/jmbc.v1i2
Core Subject : Science, Agriculture, Social,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Environmental Science Immunology & microbiology
The journal prioritizes papers in the following areas: Basic and Applied Microbiology Biotechnology and its applications in various aspects of life Bioinformatics Microbiome Molecular Biology and Genetics Conservation of Biota and Environment All organisms related to microbial communities, including fungi, plants, animals, humans, and viruses In line with the multidisciplinary nature of microbiology, biotechnology, and conservation.
Arjuna Subject : Imunologi dan Mikrobiologi (semua kategori) - Mikrobiologi dan Bioteknologi Terapan
Articles 42 Documents
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Molecular Identification of Coal Isolate Fungi Based on Internal Transcribed Spacer (ITS) Region Sequence Shinta Wulansari; Muhammad Shobihul Khoir
Journal of Microbiology, Biotechnology and Conservation Vol. 2 No. 2 (2026): Journal of Microbiology, Biotechnology and Conservation (jMBC)
Publisher : Master’s Program in Biology, Faculty of Mathematics and Natural Sciences, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/y23xf959

Abstract

Fungi represent a highly diverse group of microorganisms found in various environments, including extreme habitats such as coal deposits. This study aimed to identify fungal species isolated from Indonesian coal based on ITS region sequence analysis and compare the effectiveness of CTAB/NaCl and microwave methods for fungal DNA isolation from coal substrates. Fungal isolates were obtained from coal samples using the dilution method on PDA media. DNA isolation was performed using CTAB/NaCl and microwave methods with power levels 700 W. ITS region amplification used PCR with ITS5-ITS4 primers. Sequence analysis was conducted using BLASTn and phylogenetic reconstruction using the Neighbor-Joining method with 1000x bootstrap replicates. Two fungal isolates (isolate A and B) were obtained, with only isolate A further characterized. Morphological observations showed characteristics similar to the genus Penicillium. The CTAB/NaCl method successfully yielded DNA with purity of 2.01 and concentration of 275.89 ng/µL, producing a single DNA band on electrophoresis. Conversely, the microwave method failed to isolate intact DNA across high power, indicated by absence of DNA bands despite spectrophotometric purity values (2.02-3.28) falling within acceptable range. ITS region amplification of isolate A successfully produced a 592 bp product. BLASTn analysis revealed isolate A shared 94% identity with several Penicillium species. Phylogenetic analysis demonstrated isolate A was closely related to Penicillium sublateritium JX841245.1 (94.66% identity; 68% bootstrap support). The identity value below 97% suggests isolate A has potential as a novel or cryptic species within genus Penicillium. This study represents the first report on molecular identification of fungi from Indonesian coal and comparison of DNA isolation methods on this substrate. The CTAB/NaCl method proved superior to the microwave method for fungal DNA isolation from coal. Further analysis with additional genetic markers is needed to confirm the taxonomic status of isolate A.
Effect of Feeding Frequency of Ulva lactuca-Supplemented Diet on Growth Performance and Feed Efficiency of Nile Tilapia (Oreochromis niloticus) Chrisna Gidalti Upu; Ridwan Tobuku; Immaria Fransira
Journal of Microbiology, Biotechnology and Conservation Vol. 2 No. 2 (2026): Journal of Microbiology, Biotechnology and Conservation (jMBC)
Publisher : Master’s Program in Biology, Faculty of Mathematics and Natural Sciences, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/tabhb544

Abstract

This study aimed to evaluate the effect of feeding frequency of a diet supplemented with 12% Ulva lactuca meal on the growth performance, feed efficiency, and survival rate of Nile tilapia (Oreochromis niloticus). The experiment was conducted for 60 days at the Noekele Fish Hatchery Center, Kupang Regency, Indonesia. A Completely Randomized Design (CRD) was employed with three feeding frequency treatments and three replicates: P1 (feeding twice daily), P2 (feeding three times daily), and P3 (feeding four times daily). Parameters observed included weight gain, specific growth rate (SGR), feed efficiency, survival rate, and water quality. Data were analyzed using one-way analysis of variance (ANOVA) followed by the Least Significant Difference (LSD) test at a 95% confidence level. The results showed that feeding frequency significantly affected growth performance, feed efficiency, and survival rate of Nile tilapia (P<0.05). The highest performance was obtained in treatment P3, with a weight gain of 7.72 ± 0.77 g, specific growth rate of 2.41 ± 0.15% day⁻¹, feed efficiency of 76.66 ± 5.58%, and survival rate of 100%. These values were significantly higher than those observed in P1 and P2. Increased feeding frequency likely improved nutrient availability and utilization, resulting in enhanced growth and feed conversion efficiency. Water quality parameters remained within the optimal range for Nile tilapia culture, with temperatures of 26–30°C and pH values of 7.0–8.5 throughout the study. In conclusion, feeding Nile tilapia with a diet containing 12% Ulva lactuca meal four times per day is the optimal feeding frequency to improve growth performance, feed efficiency, and survival rate

Page 5 of 5 | Total Record : 42

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