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INDONESIA
Journal of Microbiology, Biotechnology and Conservation
Published by Universitas Mataram
ISSN : -     EISSN : 3090708X     DOI : https://doi.org/10.29303/jmbc.v1i2
Core Subject : Science, Agriculture, Social,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Environmental Science Immunology & microbiology
The journal prioritizes papers in the following areas: Basic and Applied Microbiology Biotechnology and its applications in various aspects of life Bioinformatics Microbiome Molecular Biology and Genetics Conservation of Biota and Environment All organisms related to microbial communities, including fungi, plants, animals, humans, and viruses In line with the multidisciplinary nature of microbiology, biotechnology, and conservation.
Arjuna Subject : Imunologi dan Mikrobiologi (semua kategori) - Mikrobiologi dan Bioteknologi Terapan
Articles 30 Documents
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APLIKASI PUPUK KOHE KAMBING YANG DIFERMENTASI DENGAN BIOAKTIVATOR Streptomyces sp. DALAM MENGENDALIKAN PENYAKIT BERCAK UNGU DAN MATI PUCUK PADA TANAMAN BAWANG PUTIH (Allium sativum L) Damayanti , Elsa; Haryanto, Hery; Muthahanas, Irwan
Journal of Microbiology, Biotechnology and Conservation Vol. 1 No. 2 (2025): Journal of Microbiology, Biotechnology and Conservation (jMBC)
Publisher : Master’s Program in Biology, Faculty of Mathematics and Natural Sciences, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jmbc.v1i2.6225

Abstract

This study aims to determine whether the application of fermented goat manure with Streptomyces sp. bioactivator can control purple blotch (Alternaria porri) and top dieback (Phytophthora porri) diseases in garlic plants (Allium sativum L). The research was conducted at the Microbiology Laboratory of the Faculty of Agriculture, University of Mataram, and on a farmer's paddy field located in Sembalun Bumbung Village, East Lombok Regency. The study was carried out from May to November 2024. The experimental design used in this study was a Completely Randomized Design (CRD) consisting of six treatments: P0 (no fertilizer), P1 (goat manure without bioactivator), P2 (goat manure + SH isolate), P3 (goat manure + BSi isolate), P4 (goat manure + BSc isolate), and P5 (goat manure + SH isolate + liquid SH). Each treatment was repeated six times, resulting in 36 experimental units/plots. The data were analyzed using Analysis of Variance (ANOVA) at a 5% significance level, followed by the Honest Significant Difference (HSD) test at a 5% significance level. The results showed that applying fermented goat manure with Streptomyces sp. bioactivator affected the growth of garlic plants but did not affect the yield of garlic plants (Allium sativum L). The treatment with goat manure + BSi isolate (P3) showed the highest value in plant growth, while the treatment with goat manure + SH isolate + liquid SH (P5) showed the highest yield. Meanwhile, the incidence of purple blotch disease was not affected by the application of fermented goat manure with Streptomyces sp., but it successfully reduced the incidence of top dieback disease in garlic plants.
Mangrove Conservation Model Based on Pentahelix Approach: Lesson Learned from Various Countries Bai’ul Hak, Muhamad; Nuryadin, Rusmin; Furkan, Al; M. Suhardi, Rizal; Suryadina Karthika, Nyoman
Journal of Microbiology, Biotechnology and Conservation Vol. 1 No. 2 (2025): Journal of Microbiology, Biotechnology and Conservation (jMBC)
Publisher : Master’s Program in Biology, Faculty of Mathematics and Natural Sciences, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jmbc.v1i2.6348

Abstract

Mangrove ecosystems play a crucial role in coastal protection, biodiversity conservation, and climate change mitigation. However, their degradation due to deforestation, land conversion, and pollution remains a significant challenge. This study proposes a mangrove conservation model based on the Penta helix approach, which integrates the collaboration of five key stakeholders: government, private sector, academia, community, and media. The model emphasizes a multi-stakeholder synergy to enhance policy implementation, sustainable economic incentives, scientific research, local engagement, and public awareness. Through qualitative analysis and case studies, this research identifies challenges such as regulatory gaps, conflicting interests, and limited community participation. The findings suggest that a well-coordinated Pentahelix framework can strengthen conservation efforts, promote sustainable livelihoods, and improve mangrove ecosystem resilience. The study concludes that effective governance, corporate responsibility, scientific contributions, community empowerment, and media advocacy are essential for the long-term sustainability of mangrove conservation.
Keanekaragaman Hama Pascapanen pada Komoditas Buah Tropis di Pasar Duman, Lombok Barat Amrul Jihadi; Hari Aditia Pratama, Muhammad; Aulia Rizkika, Gardena
Journal of Microbiology, Biotechnology and Conservation Vol. 1 No. 3 (2025): Journal of Microbiology, Biotechnology and Conservation (jMBC)
Publisher : Master’s Program in Biology, Faculty of Mathematics and Natural Sciences, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jmbc.v1i3.6165

Abstract

This study aims to identify post-harvest fruit pests at Duman Market, West Lombok. The research was conducted at Duman Market, Lingsar Subdistrict, West Lombok Regency, West Nusa Tenggara, using a survey method through direct observation of infested fruits. Ten fruit commodities showing pest infestation symptoms were examined, with parameters including pest types and associated symptoms. The results revealed three pest species attacking fruits at Duman Market, namely Noorda albizonalis, Bactrocera spp., and Tirathaba ruptilinea. These findings highlight the importance of post-harvest pest management in maintaining fruit quality and minimizing economic losses, particularly in traditional markets where diverse fruit commodities are traded. The study also provides baseline data for future research and pest control strategies, supporting efforts to strengthen local agribusiness and improve the competitiveness of Indonesian tropical fruits in domestic and international markets.
Respon Pertumbuhan Udang Vaname (Litopenaeus vannamei) terhadap Pakan Ekstrak Daun Jarak Pagar (Jatropha curcas) dengan Dosis Berbeda Ansani Takwin, Bagus; Hari Aditia Pratama, Muhammad
Journal of Microbiology, Biotechnology and Conservation Vol. 1 No. 3 (2025): Journal of Microbiology, Biotechnology and Conservation (jMBC)
Publisher : Master’s Program in Biology, Faculty of Mathematics and Natural Sciences, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jmbc.v1i3.7399

Abstract

Pacific white shrimp (Litopenaeus vannamei) is a leading aquaculture commodity in Indonesia, valued for its high nutritional content, rapid growth, and disease resistance. However, pathogenic infections such as Vibrio spp. can cause mortality rates of up to 100% within a day, necessitating strategies to enhance immune function. This study aimed to evaluate the effect of incorporating Jatropha curcas leaf extract into feed on the growth performance and survival of L. vannamei. The experiment was conducted for 60 days using a Completely Randomized Design with four treatments: control (no extract), 1%, 2%, and 3% J. curcas leaf extract, each with three replicates. Parameters measured included absolute weight gain, absolute length gain, specific growth rate (SGR), feed conversion ratio (FCR), and survival rate (SR). Results showed that the 3% treatment (JC3) achieved the highest absolute weight gain (6.37 g), significantly higher (p<0.05) than the control and JC1, but not significantly different from JC2. The highest SGR was also recorded in JC3, while the lowest FCR (1.2) was observed in JC3, which was not significantly different from the control. The improved growth performance with J. curcas supplementation is likely attributed to its bioactive compounds, such as tannins and flavonoids, which possess antibacterial and immunostimulant properties, thereby enhancing shrimp health and feed utilization efficiency. This study recommends a 3% inclusion level of J. curcas leaf extract in feed as an optimal dose to support the growth of L. vannamei.
Populasi Kutu Kebul (Bemisia sp.) dan Gejala Penyakit Virus pada Berbagai Varietas Kentang di Desa Sembalun Andalisa, Rani; Sarjan, Muhammad; Stella Petrunella Thei, Ruth
Journal of Microbiology, Biotechnology and Conservation Vol. 1 No. 3 (2025): Journal of Microbiology, Biotechnology and Conservation (jMBC)
Publisher : Master’s Program in Biology, Faculty of Mathematics and Natural Sciences, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jmbc.v1i3.7401

Abstract

This study aimed to determine the presence of whitefly pests (Bemisia sp.) and the symptoms of virus diseases caused by them on several potato varieties in Sembalun Village. The research was conducted from April to June 2021 in Sembalun Timba Gading Village, Sembalun District, East Lombok Regency, West Nusa Tenggara Province. The study used an experimental design with four potato varieties: Mc. Russet (A), Ranger Russet (B), Chitra (C), and Atlantik (D). Each treatment was replicated six times, resulting in a total of 24 experimental units. The results showed that the Mc. Russet and Ranger Russet varieties were more resistant to Bemisia sp. infestations in terms of both population and attack intensity. Whitefly populations fluctuated during the observation period, with the highest recorded at 49 days after planting (DAP) on the Chitra variety (2.08 insects/plant), and the lowest on Mc. Russet (1.33 insects/plant). The highest pest attack intensity was also observed at 49 DAP on the Chitra variety (1.0%), while the lowest was on Mc. Russet (0.4%). The highest incidence of yellow virus disease, transmitted by whiteflies, was recorded at 59 DAP on the Chitra variety (4.2%), and the lowest on the Ranger Russet variety (1.6%).
Analisis Populasi dan Tingkat Serangan Liriomyza huidobrensis pada Empat Varietas Kentang Industri di Wilayah Sembalun, Lombok Timur Malasari; Sarjan, Muhammad; Kisman
Journal of Microbiology, Biotechnology and Conservation Vol. 1 No. 3 (2025): Journal of Microbiology, Biotechnology and Conservation (jMBC)
Publisher : Master’s Program in Biology, Faculty of Mathematics and Natural Sciences, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jmbc.v1i3.7402

Abstract

This study aimed to analyze the population dynamics and attack intensity of the leafminer fly Liriomyza huidobrensis on four industrial potato varieties cultivated in Sembalun, East Lombok. A field experimental method was employed using a Randomized Block Design (RBD) consisting of four treatments, namely Mc. Russet (A), Ranger Russet (B), Chitra (C), and Atlantic (D), each replicated six times to obtain 24 experimental units. The results revealed that Mc. Russet and Ranger Russet exhibited greater resistance to L. huidobrensis compared to Chitra and Atlantic. The population of the leafminer fluctuated throughout the observation period, with the highest population recorded at 63–70 days after planting (DAP) on the Atlantic variety (2.22 individuals per plant), while the lowest was observed on Mc. Russet (1.14 individuals per plant). Similarly, the intensity of attack varied among varieties, with the highest recorded on Atlantic at 63–70 DAP (4.65%) and the lowest on Mc. Russet (3.1%). These findings confirm the existence of varietal differences in resistance to L. huidobrensis and provide important implications for varietal selection in integrated pest management strategies in the Sembalun region.
Pengaruh Beberapa Jenis Fermentor terhadap Kandungan Protein Kasar, Serat Kasar, dan Lemak Kasar Jerami Jagung Malaya Khaerul Hardi, Lalu; Yanuarianto, Oscar; Al Gifari, Zaid
Journal of Microbiology, Biotechnology and Conservation Vol. 1 No. 3 (2025): Journal of Microbiology, Biotechnology and Conservation (jMBC)
Publisher : Master’s Program in Biology, Faculty of Mathematics and Natural Sciences, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jmbc.v1i3.8277

Abstract

This study aimed to evaluate the effect of different fermentors on the crude protein (CP), crude fat (CF), and crude fiber (CFb) content of fermented corn straw as an alternative feed for ruminants. The experiment was conducted at the Laboratory of Animal Nutrition and Feed Science, Faculty of Animal Husbandry, University of Mataram, using a Completely Randomized Design (CRD) with four treatments and three replications: P0 (untreated/control), P1 (fermented with 1.5% EM4), P2 (fermented with 1.5% MA-11), and P3 (fermented with 1.5% Starbio). Fermentation lasted 21 days, followed by proximate analysis. The results showed that the type of fermentor significantly affected (P < 0.05) the nutrient composition of corn straw, with an increase in CP and CF, and a decrease in CFb. EM4 yielded the highest CP content (4.73%), while Starbio produced the highest CF content (1.42%) and the lowest CFb content (31.79%). In conclusion, fermentation using Starbio was the most effective in improving the nutritional quality of corn straw, particularly through enhanced CP and CF levels and reduced CFb content
Isolasi Bakteri Selulolitik Pada Ayam Kampung (Gallus gallus domesticus) Sebagai Kandidat Probiotik Ternak Unggas Aidil Fitriyan Fadjar Suryadi, Muh.
Journal of Microbiology, Biotechnology and Conservation Vol. 1 No. 3 (2025): Journal of Microbiology, Biotechnology and Conservation (jMBC)
Publisher : Master’s Program in Biology, Faculty of Mathematics and Natural Sciences, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jmbc.v1i3.8278

Abstract

This study aims to isolate cellulolytic bacteria derived from local chickens (Gallus gallus domesticus) and evaluate their potential in degrading cellulose in test media (CMC). Isolation was performed from the gastrointestinal tract of local chickens, which is expected to contain microorganisms with cellulose-degrading capabilities. The results of this study showed that the isolated bacteria can effectively break down cellulose, indicating their potential as probiotics that can help improve digestive health in poultry. Cellulolytic activity was tested by measuring the clear zone formed on agar media containing cellulose. The U5 bacterial isolate exhibited the highest ability to degrade cellulose substrate on agar media, marked by a clear zone of 35 mm, followed by U3 with a zone size of 33 mm. The formation of a clear zone on the CMC test medium indicates the presence of probiotic bacterial candidates, which need to be further tested for their biochemical characteristics to determine the biochemical properties of each bacterial candidate.
Anatomi Organ Vegetatif Tumbuhan Rija-Rija (Scleria melaleuca) di Kawasan Hutan Kemasyarakatan (HKM) Pusuk Lestari, Lombok Barat Emilia Sajida, Adinda; Kesy Juliani, Baiq; Zulifa Hemidia, Baiq; Rizki Fitri, Siti; Muhamad Azis Maulana, R.; Sukenti, Kurniasih; Mulyaningsih, Tri
Journal of Microbiology, Biotechnology and Conservation Vol. 1 No. 1 (2025): Journal of Microbiology, Biotechnology and Conservation (jMBC)
Publisher : Master’s Program in Biology, Faculty of Mathematics and Natural Sciences, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jmbc.v1i1.5998

Abstract

Cyperaceae is a plant species that belongs to the sedges family and is characterised by a triangular stem. The sedges plant is frequently observed in rice fields and under the shade of trees, including Scleria melaleuca.The objective of this study is to ascertain the anatomical characteristics of S. melaleuca.The study employed a descriptive exploratory research method and was conducted at the Advanced Biology Laboratory, Faculty of Mathematics and Natural Sciences, University of Mataram. The S. melaleuca plant samples were collected from the Hutan Kemasyarakatan (HKM) Pusuk Lestari Area in Batu Layar, West Lombok Regency, West Nusa Tenggara. The samples included the root organs, stems, leaves and seeds. The samples were preserved in 70% alcohol and sliced using the hand-free section method. The results of the study demonstrated that the S. melaleuca exhibited distinctive characteristics, including the presence of bulliform cells between the leaf epidermis, the presence of both long and short cells, closed concentric vascular bundles in the stems, a triangular stem shape, and amphicribal vascular bundles in the roots.
Karakterisasi Hama Baru Spodoptera Frugiperda Pada Tanaman Jagung (Zea mays L.) Di Lombok Barat Juwanda, Erwin; Supeno, Bambang; Haryanto, Hery; Hari Aditia Pratama, Muhammad
Journal of Microbiology, Biotechnology and Conservation Vol. 1 No. 1 (2025): Journal of Microbiology, Biotechnology and Conservation (jMBC)
Publisher : Master’s Program in Biology, Faculty of Mathematics and Natural Sciences, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jmbc.v1i1.6062

Abstract

Maize (Zea mays L.) is the main food crop in Indonesia after rice, but is vulnerable to attack by plant pests (OPT), including Spodoptera frugiperda, an invasive insect that attacks corn plants. This research aims to observe the population, level of attack and characteristics of the pest of S. frugiperda in West Lombok Regency. Based on observations, the eggs of this pest are brownish white, round in shape, and have an average size of 0.33 mm × 0.47 mm. The male pupa is reddish brown with dimensions of 14.25 mm × 4.32 mm, while the female pupa is larger, namely 17.53 mm × 5.13 mm. Larvae are found in various instars (2-6) with sizes from 1.53 mm to 35.16 mm, and colors varying from light green to dark brown. Male imagos are smaller than females, with a length of 12.45 mm and a wingspan of 27.73 mm, while female imagos have a size of 14.38 mm and a wingspan of 31.47 mm. The highest population was found at location 3 with 123 larvae, while the lowest population was at location 13 with 1 larva. The highest attack rate occurred at location 7 (50%) and the lowest at location 11 (5%). The observation method includes assessing the level of damage based on the attack intensity score (0–4). This research proposes further studies to understand the distribution and control mechanisms of S. frugiperda in order to minimize its impact on corn cultivation, especially in Nusa Tenggara Barat, Indonesia.

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