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Contact Name
Eko Didik Widianto
Contact Email
rumah.jurnal@live.undip.ac.id
Phone
+6281390576830
Journal Mail Official
jurnalbiologi@live.undip.ac.id
Editorial Address
Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro, Semarang
Location
Kota semarang,
Jawa tengah
INDONESIA
Jurnal Akademika Biologi
Published by Universitas Diponegoro
ISSN : -     EISSN : 26219824     DOI : -
Core Subject : Science,
Jurnal Akademika Biologi diterbitkan oleh Departemen Biologi Fakultas Sains dan Matematika Universitas Diponegoro Semarang. Jurnal ini sebagai media publikasi hasil karya ilmiah lulusan S1 Departemen Biologi. Jurnal Akademika Biologi menerima artikel-artikel yang berhubungan dengan bidang ilmu biologi.
Articles 234 Documents
KEANEKARAGAMAN DAN KEMELIMPAHAN LARVA INSEKTA AKUATIK SEBAGAI BIOINDIKATOR KUALITAS AIR DI SUNGAI GARANG, SEMARANG Sitta Maulina Marpaung; Fuad Muhammad; Jafron Wasiq Hidayat
Jurnal Akademika Biologi Vol. 3 No. 4 Oktober 2014
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

River is a natural habitat of macrobentik organism which is include the larvae of aquatic insects. The larvae of aquatic insects can be used as bioindicators of water pollution because they are can accomodate the change of the environmental. The purpose of this research is determine the community structure of aquatic insect larvae as well as its aspect bioindicators. This research used survey method and sampling techniques by purposive sampling. Location of the research consisted of four stations determined based on area’s function along the Garang River. At each station represent by three samples taken with surber mesh size 25 x 40 cm. The result this research showed that the larvae of insects were found in every observation station which is consists 15 genera, i.e namely Heptagenia, Stenacron, Baetis, Cloeon, Procleon, Caenis, Glossosoma, Hydropsyche, Cheumatopsyche, Chimarra, Tinodes, Chironomus, Chrysop, Isoperla and one of the family Culicidae was unidentified. The highest diversity found at Gebugan village ) in the rice fields (the diversity value of 2.17. The lower diversity at Tinjomoyo street with only reached diversity make 1,45. The conclusions of this study grouped Garang River that be into two categories, they are moderately polluted and contaminated. Heptagenia and Baetis categorized as positive bioindicator forests, fields and settlements, where as negative bioindicator industrial area are Procleon and Glossosoma. Keywords : Aquatic insect larvae , Garang River , Bioindicator
Ekspresi Gen Penyandi Peroksidase Cabai Merah (Capsicum Annuum L.) (Caper) sebagai Respons terhadap Fusarium Oxysporum Athried Elsima; Rejeki Siti Ferniah; Hermin Pancasakti Kusumaningrum
Jurnal Akademika Biologi Vol. 8 No. 2 Juli 2019
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Cabai merah (Capsicum annuum L.) merupakan komoditas pertanian penting di Indonesia yang sering terkena serangan OPT ( organisme pengganggu tanaman) misalnya Fusarium oxysporum. Tanaman cabai merah memiliki sifat ketahanan kimiawi aktif yaitu mengekspresikan peroksidase sebagai respons terhadap infeksi patogen. Penelitian ini bertujuan untuk mengetahui ekspresi gen penyandi peroksidase pada tanaman cabai merah sebagai respons terhadap jamur F. oxysporum. Penelitian ini diawali dengan inokulasi jamur pada tanaman cabai merah dilakukan dengan metode rendaman akar. Analisis ekspresi gen penyandi peroksidase menggunakan qRT-PCR dilakukan pada gen CaPer dengan gen 18s rRNA sebagai gen pembaku, pada interval 6, 48, dan 96 jam setelah inokulasi. Hasil penelitian gen CaPer terekspresi paling tinggi pada 6 jam setelah inokulasi. 
KERAGAMAN GENETIK JAHE (Zingiber officinale Roscoe) MENGGUNAKAN TEKNIK PENANDA MOLEKULER Random Amplified Polymorphic DNA (RAPD) Choirul Rizka Putri; Hermin Pancasakti Kusumaningrum; Endang Kusdiyantini
Jurnal Akademika Biologi Vol. 5 No. 2 April 2016
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Ginger plant in Indonesia is known there are three kinds of varieties, namely red ginger, emprit ginger and big ginger. Ginger (Zingiber officinale Roscoe) is one of the medicinal plants that could potentially be produced in increasing quantities and wide distribution in Indonesia that may affect patterns of genetic diversity. This study aims to determine patterns of genetic diversity of three varieties of ginger (red ginger, big ginger and emprit ginger) obtained from BALITRO, Bogor and BPTP, Ungaran using two primers (OPA1 and OPA2). Techniques of molecular markers RAPD (Random Amplified Polymorphic DNA) is one of the techniques used for the analysis of genetic diversity. The study begins by isolating DNA from 30 samples of ginger leaves, then amplification with primers OPA1 and OPA2 which then results of electrophoresis converted into the form of binary data and phylogenetic tree construction with 2:02 NYTSYS program. The results obtained are worth a total of 20 polymorphic band (86.9%) and 3 pita monomorphic (13%) with primary OPA1 and OPA2. Heterozygosity values in a row is worth 0.9026 and 0.889. Distance genetic similarities in this sample has the highest value is 1 and the lowest is 0.3. The relationship between the three varieties of big ginger are distant relationship with red ginger and emprit ginger. Red ginger and emprit ginger still have a close genetic relationship. Key Word               : Genetic Diversity, Zingiber officinale, RAPD
IDENTIFIKASI MOLEKULER TANAMAN PISANG RAJALAWE BERDASARKAN GEN INTERNAL TRANCRIBED SPACER (ITS) Firly Putri Fardilla; Hermin Pancasakti Kusumaningrum; w wijanarka
Jurnal Akademika Biologi Vol. 6 No. 1 Januari 2017
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Banana is one type of horticultural commodities in a group of fruits that have a socio-economic value is high enough for the people of Indonesia. Bananas have different varieties, one banana type Rajalawe found in Central Java. Rajalawe molecular identification has not been done before. This study aims to determine the result of the identification of the molecular basis of  Rajalawe based on genes Internal transcribed spacer (ITS), in search of identity and kinship Rajalawe. The study was conducted by isolating DNA using a method Rajalawe Doyle & Doyle, followed by ITS gene amplification and sequencing analysis. The results of gene amplification ITS produce PCR product of 643 bp. The base sequence of the sequencing results are used for the construction of phylogenetic trees. Sequence similarity analysis Rajalawe show 95% homology with Musa balbisiana and alkaline difference of 1%. Phylogenetic tree analysis showed Rajalawe have a close relationship with Musa balbisiana. However, bananas Rajalawe has several different characters with Musa balbisiana with different base sequences by 5% whereas the base sequence homology between the banana Musa balbisiana and Rajalawe with 95%.Keywords: Molecular Identification, Pisang Rajalawe, Universal Primer ITS, Musa balbisiana.
Kualitas Daun Binahong (Anredera cordifolia) pada Suhu Pengeringan Berbeda Hesti Fajar Utami; Rini Budi Hastuti; Endah Dwi Hastuti
Jurnal Akademika Biologi Vol. 4 No. 2 April 2015
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Binahong leaf is one of the plants that can be used as a traditional medicine. An important step in the use of leaf binahong as drugs is by drying. This study aims to determine the effect of different drying temperature treatment on quality of leaf binahong. The research is divided into two stages: 1. to investigate binahong leaf quality of weight loss changes, the color and texture  of the leaves binahong after different drying; 2. to investigate flavonoid quality changes after different drying. The study design used was completely randomized design (CRD) with one factor, namely the factor of temperature drying 270C,300C  temperature,  the  temperature  of  400C  and  500C  temperature  for  1  day.  The parameters of this study consisted of weight loss percentage, percentage of water content, leaf  color,  leaf  texture  and  flavonoid  content  analysis.  Analysis  of  the  data  used  isAnalysis  of  Variance  (ANOVA)  followed  by  Duncan's  real  different  test  at  95%significance level. The results showed that the drying temperature tends to increase the rate of weight loss, eliminating moisture, affect change in color and texture of the leaves and reduce the content of flavonoids. Drying temperature 500C showed the best results in reducing weight loss  and moisture content, but is not able to maintain the color and texture of the leaves and leaf flavonoid content binahong, while the temperature of 270C, a temperature of 300C and 400C temperature is able to maintain the color and texture of the leaves but slightly lowers weight  loss  and levels water. The highest binahong leaf flavonoid  produced  at  a  temperature  of  270C  is  equal  to  10,729%  followed  by  a temperature of 300C at 1,305%, 0,753%  temperature of 400C and 500C temperature of0,651, so  it  is  recommended  to  use  as  a traditional  medicine,  the  leaves  should be consumed directly binahong. Keywords: Anredera cordifolia, drying temperature, flavonoid
UJI AKTIVITAS ENZIM FITASE YANG DIHASILKAN OLEH Aspergillus niger DAN Neurospora sp. PADA KONDISI FERMENTASI YANG BERBEDA Devi Ayu Ningsih; Endang Kusdiyantini; Budi Raharjo
Jurnal Akademika Biologi Vol. 6 No. 4 Oktober 2017
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Phytase (E.C 3.1.3.8) is an important enzyme to overcome high phytic acid in livestock feed since this enzyme has the ability to hydrolyze phytic acid contained in feed material into inositol, glucose and organic phosphorus compound. This phytase enzyme can be produced by Aspergillus niger and Neurospora sp. The purpose of this study is to obtain the condition of fermentation by Aspergillus niger dan Neurospora sp. which is optimal in the production of phytase enzyme.The treatment of fermentation condition includes different pH, temperature and addition of C source (glucose). The production of enzyme was carried out in media containing glucose KCl, MgSO4.7H20, K2HPO4, NaOH with the addition of Palm Oil Mill Effluent. Mold was grown in liquid medium and measured the activity of phytase enzyme using spectophotometry. This study used a Complete Randomized Design factorial pattern with two factors. The first assaying ANOVA used a variation in the concentration of Palm Oil Mill Effluent with pH while, the second assaying used a variation in the concentration of Palm Oil Mill Effluent with temperature, and third assaying used a variation of glucose concentration with incubation times. Each factor was repeated 3 times. The data obtained were then analyzed using Analysis of Variance (ANOVA). The result of observation showed optimal phytase enzyme activity for A. Niger at pH 5 onthe temperature of 350C as much as 0.241 and 0.258 U U/ml while Neurospora sp. at pH 6 on the temperature of 350C as much as 0,273 and 0,253 U/ml. The addition of glucose experienced optimal phytase activity for Aspergillus niger as much as 0,099 U/ml at incubation time in 96 hours and Neurospora sp. as much as 0,152 U/ml in 48 hours will then decrease at subsequent incubation time.  Keywords: phytase, Palm Oil Mill Effluent, pH, A. niger and Neurospora sp.
PEMBERIAN KOMBINASI MIKROMINERAL (Cu, Fe, Zn, Co) DAN VITAMIN (A, B1, B12, C) MELALUI DRINKING WATER TERHADAP PERTUMBUHAN PUYUH ( Coturnix-coturnix japonica L) Sofatin Fitriyani; Koen Praseno; Silvana Tana
Jurnal Akademika Biologi Vol. 3 No. 3 Juli 2014
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

This aim of this research was to determine the potential of giving micromineral solution (Cu, Fe, Zn, Co) and vitamins (A, B12, B1, C) as drinking water by somatometrik. The method used is a combination gift mikromineral (Cu, Fe, Zn, Co) and vitamins (A, B12, B1, C) with normal doses, twice the normal dose, four times the normal dose, and which is a combination mikromineral and vitamins. The parameters in this research were body weight and somatometrik. This research used Completely Randomized Design (CRD). The data were analyzed with Analysis of variants (ANOVA) with the help of the SAS program, the results of which showed significant, then proceed to further test, using Duncan test. The analysis showed significant difference in body weight gain, femur length, tibia length of tarsus and metatarsus Tarso length, and do not differ significantly in the length of the beak, wing length, feed consumption and water consumption. Giving combination mikromineral (Cu, Fe, Zn, Co) and vitamins (A, B12, B1, C) as a drinking water has the potential to be used as an alternative in the management of quail with respect to maintenance doses given.Key words : vitamins, microminerals, growth, quail (Coturnix coturnix japonica L.)
Struktur Komunitas Plankton di Kawasan Wana Wisata Curug Semirang Kecamatan Ungaran Barat, Semarang Delliana Ratna Sari; Jafron Wasiq Hidayat; Riche Hariyati
Jurnal Akademika Biologi Vol. 7 No. 4 Oktober 2018
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Tujuan penelitian untuk mengetahui struktur komunitas plankton dan  mengetahui faktor-faktor fisika dan kimia yang berpengaruh dalam struktur komunitas plankton di Semirang. Penelitian ini dilakukan pada 6 stasiun dengan kondisi badan air yang berbeda. Hasil penelitian menujukkan bahwa jumlah jenis plankton yang diperoleh yaitu 12 spesies dalam  Divisi Bacillariophyta. Jenis Plankton yang banyak ditemukan adalah  Achnanthes sp, Cymbella tropica dan Cocconeis pediculus. Indeks kemelimpahan relatif termasuk ke dalam kategori jenis dominan. Indeks keanekaragaman plankton sebagian besar termasuk ke dalam katagori sedang. Indeks pemerataan termasuk ke dalam kategori persebaran merata, tidak ada dominasi suatu jenis. Indeks similaritas sebagian besar termasuk ke dalam katagori tinggi. Faktor fisika dan kimia pada perairan ini memilki kualitas air yang baik untuk kehidupan plankton. 
UJI EFIKASI BEBERAPA ISOLAT BAKTERI ENTOMOPATOGEN TERHADAP KECOA (Orthoptera) Periplaneta americana (L.) DAN Blatella germanica (L.) DALAM SKALA LABORATORIUM Monaliza Sekar Rini; Rully Rahadian; mochamad Hadi; Deni Zulfiana
Jurnal Akademika Biologi Vol. 5 No. 2 April 2016
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Cockroaches are the insect vectors of disease that can cause adverse effects on human health. Control cockroaches excessive use of insecticides can lead to residues in the environment and resistance cockroach. Therefore it is necessary to control the use of alternatives such as by biological agents such as entomopathogenic bacteria. This research used two isolates of entomopathogenic bacteria isolated from Spodoptera litura were found dead, Bacillus thuringiensis IPBCC collection and sterile distilled water as a control. Tests conducted by the spray and bait method at a concentration of 108. The results showed that all isolates entomopathogenic bacteria used in this research can cause mortality of cockroaches. Morphological observation and Koch's postulates test showed that the mortality of cockroaches likely caused by bacterial isolates were granted. SP4 bacterial isolates using spray method provides the highest mortality was 26.67% of the P. americana and 80% against B. germanica. Isolates of B. thuringiensis using bait influence the highest mortality of 10% of the P. americana and 6.67% against B. germanica. LT-50 was the most effective method of treatment of bacterial isolates SP4 spray against B. germanica was 2 hours 30 minutes 46 seconds.Keywords: Biological control, entomopathogenic bacterial, efficacy test
UJI TOTAL GLUKOSA DAN AKTIVITAS ANTIOKSIDAN BEBERAPA PRODUK PANGAN FUNGSIONAL BERBAHAN DASAR UBI JALAR UNGU (Ipomoea batatas L.) var AYAMURASAKI Edysri Hermayudha P; Munifatul Izzati; Endang Saptiningsih
Jurnal Akademika Biologi Vol. 2 No. 2 April 2013
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Functional food is food with high nutrition and important to illness terapy. Functional food product based of purple sweet potato are an alternative to replace primary food. This research purpose to know the quantity of total glucose, antioxydant activity, and antosianin decreaseful colour in a few of functional food product based of purple sweet potato. This research take place in food technology laboratoryum Unika Semarang on Desember until January 2013. This research use RAL single factor of food making process include milk, ice cream, wet noodle, dry noodle, and flour look for quantity of total glucose, antioxidant activity, and antosianin decreaseful colour. Method of research include analyse quantity of total glucose assay fenol method, antioxidant activity assay Diphenyl Pycril Hydriazil (DPPH), and observation in antosianin decreaseful colour product. The result of research show that the best food product is purple sweet potato flour. That product has quantity of total glucose 2,805 g/100 g, IC50  - 49951 %, and antosianin colour product take no damage because it still look purple. Keyword: the functionality food product, total glucose quantity, antioxydant activity IC50, antosianin decreaseful colour