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Biodiversitas Journal of Biological Diversity
Published by Universitas Sebelas Maret
ISSN : 1412033X     EISSN : 20854722     DOI : https://doi.org/10.13057/biodiv/d230231
Core Subject : Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology
The Biodiversitas Journal was first published in 2000 by the Department of Biology, FMNS, Universitas Sebelas Maret, Surakarta, Indonesia, then in 2006 it was co-published by the Society for Indonesian Biodiversity and that department; since 2017 it was also hosted by Smujo. From 2003-2012 it was accredited by DGHE, Ministry of Education, R.I., since 2014 was indexed by Scopus, where DOAJ and Google Scholar was indexed first.
Arjuna Subject : Biokimia, Genetika dan Biologi Molekuler - Biokimia, Genetika dan Biologi Molekuler (Lain-Lain)
Articles 6,259 Documents
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A Comparison on Biodiversity between Private Conservation and Wildlife Reserve Forests in Riau by using Macro-moths as an Indicator HARI SUTRISNO
Biodiversitas Journal of Biological Diversity Vol. 10 No. 1 (2009)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d100107

Abstract

A study on biodiversity of the two forest management types, private conservation forest PT. A and wildlife reserve forest of Suaka Margasatwa Giam Siak Kecil, Riau by using a rapid assessment approach with macro-moths as an indicator wasconducted from 23 October to 6 November 2007. Four sample sites were established in Giam Siak Kecil Wildlife Reserve,whereas three sample sites were performed in private conservation forest PT. A. The results show that the diversity indexes based on Fisher’s α of the private forest PT. A was higher than those of wildlife reserve forest Giam Siak Kecil, they were 67.98 and 47.86, respectively. The species composition of the two forests is different, pyralid moths dominate at Giam Siak Kecil. On the contrary, Geometrid moths dominate at private conservation forest PT. A. The results indicated that diversity index and species composition in Giam Siak Kecil is influenced by habitat changes and decrease on floral diversity due to illegal logging. Moreover, a low faunal similarity which is indicated by Jaccard’s index that is only 0.218 showed that thesamples represent significant different communities.Key words: biodiversity, conservation, macro-moths, Fisher’s α index
The Effect of Seed Maturity, Temperature and Storage Period on Vigor of Picrasma javanica Bl. Seedling NINIK SETYOWATI
Biodiversitas Journal of Biological Diversity Vol. 10 No. 1 (2009)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d100110

Abstract

A study on the effect of seed maturity, temperature and period of storage on vigor of Picrasma javanica Bl. seedling was conducted at the Macropropagation Laboratory, Research Centre of Biology, LIPI, Cibinong, from March to September 2008. The research was arranged using Randomized Block Design with 3 factors and 3 replications, in which each replication had 20 samples. The first factor was stage of seed maturation with 2 levels i.e. pre-mature and mature stage; the second factor was storage temperature with 3 levels i.e. ambient temperature (28±1ºC), 20ºC and 5ºC; and the third factor was storage period with 4 levels i.e. 0, 1, 2, 3 months. The result showed that the germination time of Picrasma javanica seed was varies between 11-23 days. The mature seeds had better growth as compared to pre-mature seeds. Storage on the ambient temperature (28±1ºC) caused decrease of seed vigor, so is not suggested to seeds store in the ambient temperature. The storage under lower temperature (5ºC and 20ºC) was able to maintain seed vigor until 3 months storage.Key words: seeds maturity, temperature, storage period, vigor, Picrasma javanica Bl. seed.
Biodiversity of Endophytic Fungi Associated with Uncaria gambier Roxb. (Rubiaceae) from West Sumatra MUHAMMAD ILYAS; ATIT KANTI; YULIASRI JAMAL; HERTINA HERTINA; ANDRIA AGUSTA
Biodiversitas Journal of Biological Diversity Vol. 10 No. 1 (2009)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d100105

Abstract

Endophytic fungi are specific microbes that live at a specific ecosystem in nature. Various endophytic fungi have been known posses an ability to produce a broad range of biologically active substances. Totally 53 endophytic filamentous fungi were isolated from leaves, stems, fruits and roots of the two plant varieties of Uncaria gambier Roxb. (Rubiaceae), i.e. gambir udang and gambir nasi. Morphological observation of 53 isolated endophytic filamentous fungi was further divide into two classes, Coelomycetes and Hypomycetes. Fifteen fungi isolates are unidentified due to lack of specific morphological characters. Morphologically identified fungi at genera level are: Aspergillus, Cladosporium, Diaporthe, Fusarium, Penicillium, Pestalotiopsis, Phoma and Phomopsis. Chemotaxonomic analysis base on their TLC chromatogram patterns of the ethyl acetate extract are in agreement to morphological grouping.Key words: endophytic fungi, Uncaria gambier Roxb., morphological character, chemotaxonomy.
Phagocytosis Effectivity Test of Phenylbutenoid Compounds Isolated from Bangle (Zingiber cassumunar Roxb.) Rhizome CHAIRUL CHAIRUL; PRAPTIWI PRAPTIWI; SOFNIE MARUSIN CHAIRUL
Biodiversitas Journal of Biological Diversity Vol. 10 No. 1 (2009)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d100108

Abstract

This study was conducted to determine the immunostimulant activity of several phenylbutenoid derivatives isolated frombangle (Zingiber cassumunar Roxb) rhizome: [1] [(E)-4-(3’,4’-dimethoxyphenyl)but-3-en-1-ol], [2] [(E)-4-(2’,4’,5’ trimethoxyphenyl) but-3-en-1-ol] and [3] [(E)-4-(3’,4’,1-trimethoxyphenyl)but-3-en-1-ol]. Immunostimulant activity was done by stimulating macrophage cells of mouse peritoneum. The result showed that [(E)-4-(3’,4’-dimethoxyphenyl)but 3-en-1-ol] compound had highest immunostimulant activity (99.0%) compared to compound 2 (93.7%) and 3 (80.0%).Key words: Zingiber cassumunar Roxb, immunostimulant, phenylbutenoid derivative, [(E)-4-(3’,4’-dimethoxyphenyl)but-3-en-1-ol], [(E)-4-(2’,4’,5’-trimethoxyphenyl)but-3-en-1-ol]and [(E)-4-(3’,4’,1-trimethoxy phenyl)but-3-en-1-ol
Vegetative and generative growth of groundnut genotypes under biotic environmental stress AGUSTINA ASRI RAHMIANNA; ERIYANTO YUSNAWAN
Biodiversitas Journal of Biological Diversity Vol. 17 No. 2 (2016)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d170219

Abstract

Rahmianna AA, Yusnawan E. 2016. Vegetative and generative growth of groundnut genotypes under biotic environmental stress. Biodiversitas 17: 503-509. The decrease in groundnut pod yield is mainly influenced by disease infestation, especially bacterial wilt and foliar diseases. The objectives of this experiment were to determine the response and tolerance of groundnut genotypes to bacterial wilt, leaf spot and rust diseases, and seed infection by Aspergillus flavus. The planting materials were 25 genotypes (11 Indonesian cultivars, 12 lines introduced from ICRISAT, 1 Indonesian promising line, and 1 local cultivar) with various superiorities on diseases resistance. This study was arranged in a randomized completely block design with triplicate. The results indicated that both genotypes from ICRISAT and Indonesia had similar response to leaf spot i.e. ranging from susceptible (score 6-7) to highly susceptible (8-9). The score for rust ranged from moderately resistant to susceptible. The average pod yield was 23.1 g/plant (from 11.9 g to 29.5 g), and 13 and 12 genotypes produced pods higher and lower than the average value, respectively. ICGV 86158 and ICGV 95322 had the highest and lowest seeds as well as pod productivity, respectively. The ICRISAT genotypes were susceptible to Ralstonia solanacearum infection, except for ICGV 86590. Among the Indonesian cultivars, those with Valencia type of growth, relatively had better resistance to bacterial infection. These cultivars were also resistant to rust and A. flavus infection.
The Effect of Crude Extract of Pandanus conoideus Lamb. var. Yellow Fruit on Apoptotic Expression of the Breast Cancer Cell Line (T47D) OKID PARAMA ASTIRIN; MARTI HARINI; NOOR SOESANTI HANDAJANI
Biodiversitas Journal of Biological Diversity Vol. 10 No. 1 (2009)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d100109

Abstract

A mechanism controlling a growing cancer cells is by a programmed cell death (apoptosis). The wildtype-p53 enable to stop cleaves that follow DNA repair or cell death (apoptosis). The mutation of wt-p53 caused loosing its ability to inhibit cancer cells proliferation. Healing methods like surgery, radiation, immunotherapy and chemotherapy still have some weaknesses, and clinical medicine to cancer is also still has any dissatisfactory. Much of chemotherapy was not given optimal result yet, because no specific action to cancer cells only, but also to the normal cells. These problems encourage important effort to find specific and sensitive anticancer. Empirical evidence indicates that the crude extract of Pandanus conoideus Lamb var. yellow fruit has potential effect as an anticancer. Method of Freshney was used in growing T47D cell line, counting cells was done by direct counting, and apoptotic evaluation was done by TUNEL enzymatic labeling assay. The results of theresearch demonstrated that the LC50 of yellow fruit extract are 0.25 μL/mL. The percentage of apoptotic of 0.125 μL/mL,0.0625 μL/mL, and 0.03125 μL/mL are 34.38±2.26, 30.03±3.87 and 21.07±1.14 respectively.Key words: T47D, Apoptotic, p53, Pandanus conoideus Lamb. var. yellow fruit.
Short Communication: Georeferencing orchids specimen history cards in Bogor Botanic Gardens to increase their use for conservation efforts EKA MARTHA DELLA RAHAYU; SAFRAN YUSRI
Biodiversitas Journal of Biological Diversity Vol. 17 No. 2 (2016)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d170220

Abstract

Rahayu EMD, Yusri S. 2016. Georeferencing orchids specimen history cards in Bogor Botanic Gardens to increase their use for conservation efforts. Biodiversitas 17: 510-514. Orchids are considered valuable plant resource but overharvesting and habitat conversion have threatened their population. Bogor Botanic Gardens (Kebun Raya Bogor; BBG) stores millions of plant specimens, including orchids, taken from the wild or captivity. Origin of specimens is recorded in specimen tags and cards, where each of these can be converted to species occurrence datum for investigations of biodiversity, its relationship with the environment, evaluating conservation efforts and anthropogenic disturbances along spatial or temporal scales. However, data from tags and cards available are often insufficient because localities are typically being recorded as textual descriptions, without geographic coordinates, thus making analysis using Geographical Information System (GIS) tools difficult. In this paper, we reviewed the use of online resources (i.e. GoogleMapsâ„¢, ProtectedPlanet.net) for georeferencing specimen cards and Quantum GIS as a GIS tool to store and display the data. Specimen cards from the chosen genera of orchid in BBG were reviewed. The georeferencing process encountered several obstacles, includes: geographically biased locations, changes in spatial-administrative borders, unregistered location name, unavailability of location name in online resources, and typographic errors during specimen recording process. We also encounter quality difference along georeferenced records, some are good quality (i.e. record coordinates or nearest village) and some are poor (only record the provinces). Georeferencing is an underappreciated task, but once it is done, it can be used for future expeditionary research, national conservation planning, species status review, and other large scale analysis for both spatial and temporal scales.
Plant diversity after sixteen years post coal mining in East Kalimantan, Indonesia LIRIS LIS KOMARA; DEVI NANDITA CHOESIN; TATI SURYATI SYAMSUDIN
Biodiversitas Journal of Biological Diversity Vol. 17 No. 2 (2016)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d170223

Abstract

Komara LL, Choesin DN, Syamsudin TS. 2016. Plant diversity after sixteen years post coal mining in East Kalimantan,Indonesia. Biodiversitas 17: 531-538. Post coal mining areas need to be rehabilitated through reclamation and revegetation. The objective of this study was to evaluate plant diversity after 16 years of revegetation activities in a coal mining reclamation site in East Kalimantan. In an effort to restore plant diversity, the coal mining company began by planting fast growing species as pioneers, then planting local species after three years. This study compared a 20 hectare reclamation site with conditions in the pre-mining area, which covered 14,988 hectares. Vegetation sampling was conducted in 20 plots measuring 20x20 m2 along line transects, with 100 m distancebetween plots. A total of 104 plant species were found in the reclamation site, consisting of 76 tree species and 28 herbaceous species. Tree species consisted of 35 planted local species (e.g., Dryobalanops aromatica, Eusideroxylon zwageri, Macaranga gigantea), 25 planted non-local species, and 16 local species that grew spontaneously (e.g., Leucaena glauca, Lansium domesticum, Shorea laevis). In comparison, 133 species were found in the pre-mining site, consisting of 132 local tree species, one non-local tree species (Acacia mangium) and 52 herbaceous species. Tree species diversity index in the reclamation site after 16 years post mining (i.e., 3.54) was stilllower than in the pre-mining area (4.29); while the diversity indices for herbaceous plants were relatively similar (2.97 and 2.67 in the reclamation and pre-mining sites respectively). The slightly higher diversity of herbaceous plants in the reclamation site may be attributed to higher coverage per species in this site, despite lower species richness.
Identification and expression of two types of chicken GnRH-II genes in mature hard-lipped barb, Osteochilus hasselti N. A. PRAYOGO; G. E. WIJAYANTI; I. SULISTYO; P. SUKARDI
Biodiversitas Journal of Biological Diversity Vol. 17 No. 2 (2016)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d170222

Abstract

Abstract. Prayogo NA, Wijayanti GE, Sulistyo I. Sukardi P. 2016. Identification and expression of two types of chicken GnRH-II genes in mature hard-lipped barb, Osteochilus hasselti. Biodiversitas 17: xxx. Gonadotropin-releasing hormone (GnRH) is synthesized in the brain and acts in the anterior pituitary to stimulate the release of gonadotropins in fishes as well as in other vertebrates. Genomic DNAs and cDNAs of two chicken-type GnRH-II genes of hard-lipped barb, namely cGnRH-II type 1 and type 2, were cloned. The length of cloned genomic DNA of cGnRH-II type 1 was 580 bp and cDNA was 206 bp. The length of cloned genomic DNA of cGnRH-II type 2 was 570 bp and cDNA was 196 bp. The cGnRH-II type 1 and type 2 cDNAs encode precursors of 68 and 63 amino acids, respectively. Those precursors consist of a signal peptide, cGnRH-II decapeptide and a GnRH-associated peptide (GAP) linked by a Gly-Lys-Argproteolytic site. Using quantitative Real Time-PCR, expression levels of these two cGnRH-II genes were detected in the brain, liver and gonad of hard-lipped barb. Expression of the GnRH-II type 1 gene was found only in the brain and liver, on the other hand, expression of the cGnRH-II type 2 gene was found in the gonad, in addition to the brain and liver. The expression of the cGnRH-II genes outside the brain suggested that cGnRH-II might act as an autocrine or paracrine regulator.
Sequence-Related Amplified Polymorphism (SRAP) analysis for studying genetic characterization of Bouea macrophylla SOMBHAT KAEWPONGUMPAI; SUPATTRA POEAIM; ONGKARN VANIJAJIVA
Biodiversitas Journal of Biological Diversity Vol. 17 No. 2 (2016)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d170224

Abstract

Kaewpongumpai S, Poeaim S, Vanijajiva O. 2016. Sequence-Related Amplified Polymorphism (SRAP) analysis for studying genetic characterization of Bouea macrophylla. Biodiversitas 17: 539-543. Bouea macrophylla Griff. is well-known as one of native typical fruits in Southeast Asia which needs to be preserved and continuously cultivated because of economical and ecological significances. More recently, sequence-related amplified polymorphism (SRAP) markers have been developed, which are used to amplify coding regions of DNA with primers targeting open reading frames. This technique has proven to be robust and highly variable and is attained through a significantly less technically demanding process. In this research, SRAP method was preliminary applied to assess genetic characterization of B. macrophylla. Genomic DNA was extracted from fresh leaf samples. The result clearly showed that at 100 ng template DNA and MgCl2 5 mM concentration are suitable for further PCR analysis. Thirty SRAP primer combinations were initially screened for analysis and 26 primer combinations were chosen for further analysis. A total of 222 DNA fragments, varying from 90-2500 bp, were amplified. The produced band number for each optimal primer set ranged from 3 to 12 with a percentage of polymorphic bands spanning from 33.33 to 80.00%. Therefore, SRAP analysis is suitable for further analysis method on genetic study of Bouea species and related genera.

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