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Nusantara Bioscience
ISSN : 20873948     EISSN : 20873956     DOI : https://doi.org/10.13057/nusbiosci
Core Subject : Education,
Aims and Scope Nusantara Bioscience (Nusantara Biosci) encourages submission of manuscripts dealing with all aspects of biological sciences that emphasize issues germane to biological and nature conservation.
Arjuna Subject : Umum - Umum
Articles 545 Documents
Kidney histology in streptozotocin-induced diabetic male Wistar rats treated with combined extract of temulawak rhizome and belimbing wuluh fruit KARTIAWATI ALIPIN; EMA PURNAMA SARI; MADIHAH MADIHAH; TIA SETIAWATI; NINING RATNINGSIH; DESAK MADE MALINI
Nusantara Bioscience Vol. 9 No. 3 (2017)
Publisher : Smujo International

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/nusbiosci/n090312

Abstract

Alipin K, Sari EP, Madihah, Setiawati T, Ratningsih N, Malini DM. 2017. Kidney histology in streptozotocin-induced diabetic male Wistar rats treated with combined extract of temulawak rhizome and belimbing wuluh fruit. Nusantara Bioscience 9: 312-317. Complications that are occurred in patients with Diabetes Mellitus usually followed by kidney damage. Temulawak (Curcuma xanthorrhiza Roxb.) and belimbing wuluh (Averrhoa bilimbi L.) were traditionally used to decrease blood glucose level. Thus, they were potential as antidiabetic drugs. This study aimed to evaluate the combination of ethanol extracts of temulawak rhizome and belimbing wuluh fruit in repairing kidney damage in diabetic male Wistar rats induced by streptozotocin (STZ). An experimental method using a completely randomized design that consist of seven treatments with three replications. Six treatment groups were injected intraperitoneally with a dose of 60 mg/kg BW STZ, and one group served as a control. The animals which have blood glucose level ≥200 mg/dl were stated as diabetic. Furthermore, the animals were treated orally with single extract i.e. temulawak 17.5 mg/kg BW or belimbing wuluh 750 mg/kg BW and combined extracts 383.75 or 767.5 mg/kg BW, as well as glibenclamide 0.45 mg/kg BW as reference, including diabetic rat as positive control and non-diabetic rat as negative control. The results showed that combine extract at dose of 383.75 mg/kg BW treatment repaired the kidney histology, i.e., glomerular diameter and Bowman space width, as well as significantly decreased the necrosis percentage of proximal tubular in diabetic rat compared with positive control group (p<0.05). In conclusion, the combined extract of temulawak rhizome and belimbing wuluh fruit has potent to cure renal failure in diabetic rats induced by streptozotocin.
Short Communication: Infraspecific variations in essential oil compositions of Nepeta fissa from Iran SEYED MEHDI TALEBI; MAJID GHORBANI NOHOOJI; MAHBOOBEH YARMOHAMMADI
Nusantara Bioscience Vol. 9 No. 3 (2017)
Publisher : Smujo International

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/nusbiosci/n090313

Abstract

Talebi SM, Nohooji MG,Yarmohammadi M. 2017. Short Communication: Infraspecific variations in essential oil compositions of Nepeta fissa from Iran. Nusantara Bioscience 9: 318-321. Nepeta fissa C.A. Mey. is a member of Mint family (Lamiaceae) and distributed naturally in different regions of Iran. The aim of this investigation was to provide more information about the influence of environmental conditions on the essential oil composition of two populations, Polor and Dizin, of this species. The dry plant samples were submitted to hydrodistillation for 2.5 h, using a Clevenger-type apparatus. The highest amount of essential oil yields (0.2%) were obtained for Dizin population. Forty-nine compounds, representing 85.9 to 97.80% of the total essential oils, were separated and identified. In Dizin and Polor populations, the essential oils were characterized by the very high percentage of oxygenated monoterpenes (79.1%), oxygenated diterpenes (20.01%) that constituted the predominant classes, respectively. From the analyzed populations, the major compounds in Dizin population were 1, 8-Cineole, 4 a-α, 7-α, 7 a-α-Nepetalactone and 2E, 4E-nonadienal. While, phytol, caryophyllene oxide, E-caryophyllene, and thymol were the core compounds of essential in Polor population. The obtained results showed that environmental conditions are the important factors influencing the yield and also the chemical compositions of essential oils in N. fissa. Knowledge of the essential oil chemical composition in relation to ecological factors is a most prominent quality criterion for its marketing and contributes to its valorization as a functional ingredient in food industry.
Visual symptoms and control of the Red Palm Weevil (Rhynchophorus ferrugineus) in the Gaza Strip, Palestine ABDEL FATTAH N. ABD RABOU; EQBAL S. RADWAN
Nusantara Bioscience Vol. 9 No. 3 (2017)
Publisher : Smujo International

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/nusbiosci/n090314

Abstract

Abd Rabou AN, Radwan ES. 2017. Visual symptoms and control of the Red Palm Weevil (Rhynchophorus ferrugineus) in the Gaza Strip, Palestine. Nusantara Bioscience 9: 322-329. The Red Palm Weevil – RPW (Rhynchophorus ferrugineus Olivier) (Coleoptera: Curculionidae) has recently become one of the most destructive pests of Date Palms in the Gaza Strip and the Middle East. It is a serious pest threatening the Date Palm health and production, with the larva is the most destructive stage. The current study aims at introducing the visual symptoms and control techniques of the RPW in the Gaza Strip, Palestine. Field surveys and institutional visits were applied to fulfill the purpose of the study. The current study documented that first local infestation of Date Palm trees with the RPW was discovered in late 2011. The introduction of infected offshoots from Egypt through earth tunnel trade and the ability of the adult RPW to fly long distance and cross borders seem to be main causes of the local infestations with the pest. Different control techniques have been adopted by the responsible parties to combat the RPW; with the integrated pest management (IPM) program was tracked and respected. Finally, the study recommends the cooperation of different parties and authorities to adopt appropriate policies to eliminate the RPW and to support farmers with the necessary pesticides and equipment to control this painful pest.
Growth, biomass production and nutrient accumulation of Macaranga gigantea in response to NPK fertilizer application DWI SUSANTO; SRI MULYATI; HERI PURNOMO; DADDY RUHIYAT; RUDIANTO AMIRTA
Nusantara Bioscience Vol. 9 No. 3 (2017)
Publisher : Smujo International

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/nusbiosci/n090315

Abstract

Susanto D, Mulyati S, Purnomo H, Ruhiyat D, Amirta R. 2017. Growth, biomass production and nutrient accumulation of Macaranga gigantea in response to NPK fertilizer application. Nusantara Bioscience 9: 330-337. Research described in this paper investigated the effect of fertilizer application on the growth, biomass production and nutrient accumulation of mahang (Macaranga gigantea) plant components. The experiment was carried out in a research area of the Faculty of Forestry, Mulawarman University, Samarinda, East Kalimantan. The effects of five dose levels of an NPK (16-16-16) fertilizer on the growth rate of mahang seedlings planted in field conditions were compared. The treatments consisted of a control group (with no fertilizer), and dosages of 40 g, 80 g, 120 g and 160 g per plant. Fertilization at those levels was applied twice: the first application at four weeks after the planting and the second application at 6 months after the first. Growth rates between treatments were compared over a twelve month period. The research findings revealed that as the dosages of fertilizer increased, so the growth, biomass production and nutrient accumulation in components of the plants also increased. The best growth was found in the treatment in which fertilizer was applied (twice) at a dosage of 120 g per plant; at this dose level, by twelve months of age, the stem basal diameter had reached 45.24.9 cm, stem height 219.239.1 cm, number of leaves per plant 17.31.2, and canopy diameter 246.725.0 cm. The highest production of above-ground biomass was also found in the fertilizer treatment of 120 g per plant; the largest proportion of the biomass was in the leaves, followed by the wood, and then bark. The nutrient element that had accumulated most in plants at 1 year of age was potassium, followed by phosphorus and then nitrogen. The highest nitrogen uptake of stands was found in the NPK fertilization with a dosage of 160 g per tree, while the highest phosphorus and potassium uptake was found at a fertilizer dosage of 120 g per tree. The amount of nutrients accumulated in producing one tonne of above-ground biomass increased in response to different fertilization treatments. The fertilizer treatment of 120 g per plant (applied twice; the first soon after planting and the next after 6 months) resulted in the accumulation within the 1 year old plants of 2.38 kg of nitrogen, 6.36 kg of phosporus and 17.83 kg of potassium, with an N:P:K ratio of 13.3 : 35.6 : 100. The availability of the element potassium needs special attention when this species is cultivated.
Cost-benefit analysis of rehabilitation and opening new cultivation land for tangerine cv Madu impacted by volcanic ash fallout of Mount Sinabung, North Sumatra, Indonesia LYLI MUFIDAH; AGUS SUGIYATNO; M. TAUFIQ RATULE
Nusantara Bioscience Vol. 9 No. 4 (2017)
Publisher : Smujo International

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/nusbiosci/n090401

Abstract

Mufidah L, Sugiyatno A, Ratule MT. 2017. Short Communication: Cost-benefit analysis of rehabilitation and opening new cultivation land for tangerine cv Madu impacted by volcanic ash fallout of Mount Sinabung, North Sumatra, Indonesia. Nusantara Bioscience 9: 339-345. Indonesian tangerine plants which have diverse varieties possess the adaptability from lowlands to highlands in the tropical region. Some of the tangerine production centers in Indonesia, such as Karo, Mandailing Natal, Dairi, Malang, Batu, and Bali are located in the volcano path which is prone to eruption. The eruption of Mount Sinabung in 2013 and 2014 had caused damage to the local tangerine cv Madu cultivation which was estimated to reach more than 476 billion rupiahs. The eruption affected a cultivation area of 7.202,89 ha and has been predicted to be one of the factors that caused the decline of North Sumatra’s contribution to tangerine production. Therefore, this paper has tried: (i) to analyze the financial feasibility of rehabilitating tangerine cv Madu cultivation located in between 7-10 km radius of the eruption center (zone 1) and in between 5-7 km radius of the eruption center (zone 2), and (ii) to consider the option of opening up a new tangerine cultivation land.The results showed that the BCR for the immediate rehabilitation of zones 1 and 2 were 3.33 and 2, respectively. Thus, the rehabilitation scheme was feasible to be implemented and can reduce the damage and losses suffered by farmers. On the other hand, the opening of new land still takes approximately three years to be productive. The BCRof the option of opening new land within that period of time was 0.47, which is lower compared with that of the rehabilitation of zones 1 and 2 (2.61 and 2.57, respectively).This study was intended as an initial guidance for making decisions and determining the potential benefit to be gained and the losses that can be avoided. This study also gave an overview of the budget that should be prepared. In conclusion, we recommended the farmers to carry out the rehabilitation over opening up new cultivation land.
Effect of Azadirachta excelsa and Melia azedarach extracts on soybean germination RUMMI AZAHRA GUMILAR; NURHENI WIJAYANTO; ARUM SEKAR WULANDARI
Nusantara Bioscience Vol. 9 No. 4 (2017)
Publisher : Smujo International

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/nusbiosci/n090402

Abstract

Gumilar RA, Wijayanto N, Wulandari AS. 2017. Effect of Azadirachta excelsa and Melia azedarach extracts on soybean germination. Nusantara Bioscience 9: 346-351. Azadirachta excelsa and Melia azedarach are potential tree species developed in agroforestry system. Both plants are fast growing species and good for timber use. However, there is an inadequacy on both species due to their allelopathic compound contents. Allelopathic compounds in the plants are distributed from root, stem, leaf, and fruit; which at certain concentrations could inhibit germination, growth, and development of other plants. The objective of this study was to analyze the effects of root, leaf, and twig extracts of M. azedarach and A. excelsa on the germination of soybean. Result of this study showed that the root, leaf, and twig extracts of M. azedarach and A. excelsa were able to inhibit the development of soybean. 5 % twig extract of M. azedarach significantly inhibited the germination by 77.75%. Leaf and twig extracts of A. excelsa at 1.25% concentration has shown to provide stimulant effect on the germination of soybean.
Robust in vitro propagation and regeneration of ubi kuning high beta carotene cassava genotype through somatic embryogenesis SUPATMI SUPATMI; HANI FITRIANI; NURHAMIDARR RAHMAN; N. SRI HARTATI; ENNY SUDARMONOWATI
Nusantara Bioscience Vol. 9 No. 4 (2017)
Publisher : Smujo International

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/nusbiosci/n090403

Abstract

Supatmi, Fitriani H, Rahman N, Hartati NS, Sudarmonowati E. 2017. Robust in vitro propagation and regeneration of ubi kuning high beta carotene cassava genotype through somatic embryogenesis. Nusantara Bioscience 9: 352-360. Ubi kuning is a local genotype of cassava with high beta carotene content but the development of this genotype is still low because of plant disease susceptibility. Objectives of this study were to robust induce and regenerate somatic embryos of ubi kuning in vitro as well as to define a protocol of cyclic somatic embryogenesis of ubi kuning. Different size of leaf lobes, various concentration of picloram and different light conditions were tested to produce an effective and efficient somatic embryos (SEs).The best response of the induction of embryogenic callus was observed in leaf lobes explant with range size of 1-3 mm and >5mm cultured on induction medium (MS + 4% sucrose + 4 μM CuSO4 + 0.1 mM Glutamine + 0.8% Microagar) supplemented with either 10 or 18 mg/L picloram grown under dark light for 4 weeks. Retransferring embryogenic callus to the same medium supplemented with 16 mg/L picloram gave the advanced development of primary somatic embryos (PSEs) after 70 d grown under both dark and light condition treatments. A positive correlation between globular and cotyledon stages was obtained in all treatments (P≤ 0.01). The highest shoot and root growth (30% and 25%) was achieved in the regeneration of cotyledonary like-tissues cultured on callus embryogenic media (CEM) (MS basal+ 2.5 μM CuSO4 + 3% sucrose + 2.75 g/L phytagel) supplemented with 1.6 mg/L of BAP (6-Benzylaminopurine).
The effect of gelatin hydrolysate from pygostyle of broiler produced by enzymatic hydrolysis process of Aspergillus niger to prophylaxis YASMI PURNAMASARI KUNTANA; ROOSTITA BALIA; SAYUTI MURAD; YULI ASTUTI HIDAYAT; RUHYAT PARTASASMITA
Nusantara Bioscience Vol. 9 No. 4 (2017)
Publisher : Smujo International

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/nusbiosci/n090404

Abstract

Kuntana YP, Balia R, Murad S, Hidayat YA, Partasasmita R. 2017. The effect of gelatin hydrolysate from pygostyle of broiler produced by enzymatic hydrolysis process of Aspergillus niger to prophylaxis of osteoporosis white rat. Nusantara Bioscience 9: 361- 366. The pygostyle of the broiler is a cartilage tissue containing collagen, a mucoprotein being a plasma expander. The hydrolysis process of collagen by enzyme can produce gelatin hydrolysate differently compared to that from commercial gelatin because it has an amino acid composition with low average molecular weight. It becomes an advantage because it can be directly absorbed by the digestive tract. In addition, the enzymatic process can be done in short time with the low cost and also environment-friendly approach. This process can be assisted by Aspergillus niger, i.e., safe microorganisms (GRASS). A. niger has a protease enzyme that can digest collagen into gelatin hydrolysate. The enzyme is extracellularly removed so that its utilization does not require high input value expenditure. Gelatin hydrolysate can reduce the disease of osteoporosis. The objective of this study was to know the effect of gelatin hydrolysate produced from chicken bones in the favor of A. niger for increasing the benefit products. The specific targets are to find the amount of gelatin hydrolysate, precisely eliminated osteoporosis of rat (Rattus norvegicus). The experimental research was conducted in the laboratory with using complete randomized design followed by the descriptive analysis with 5 treatment and 5 replication. CCl4 dose 0,14 mL/g BW (P1 as a negative control), Potatoes dextrose Broth (PDB) dose 0,5 mL/g BW (P2 positive control), gelatin hydrolysate dose 0.25; 0.5; 0.75 mL/g BW (P3-P5). The observed parameters were including body weight and histological observation of musculoskeletal tissue in terms of measurement of fiber muscle, cartilage and collagen diameter. The test animal used was male white rat (R. norvegicus) Wistar strain aged 2-3 months with body weight 185-200 g. Data were analyzed using ANOVA and Duncan test. The results show that gelatin hydrolyzate as an environmentally friendly product serves as a prevention of immune diseases and degenerative diseases such as osteoporosis.
Short Communication: Birth weight of a Holstein calf based on forefoot circumference LIA BUDIMULYATI SALMAN; WILLYAN DJAJA; ASEP ANANG; HENI INDRIJANI; RUHYAT PARTASASMITA
Nusantara Bioscience Vol. 9 No. 4 (2017)
Publisher : Smujo International

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/nusbiosci/n090405

Abstract

Salman LB, Djaja W, Anang A, Indrijani H, Partasasmita R. 2017. Birth weight of a Holstein calf based on forefoot circumference. Nusantara Bioscience 9: 367-370. Record of birth weight of Holstein calf can be used as consideration for selection. The purpose of this study is to determine the accuracy of birth weight based on regular measuring tape compared to Calf scale BW Tape. The records of calf’s birth weight were collected from 1370 female calf birth records from PT Taurus Dairy Farm, Sukabumi. Birth weight measurements were performed by (i) measuring birth weight using Calf scale BW Tape (Nasco, Fort Atkinson, WI); (ii) predicting birth weight with regression based on the circumference of the right front toenails using regular measuring tape a day after the calf was born; (iii) weighing the calf with the scales. The results showed that the predicted birth weight of dairy cow’s calves can be done by regular measuring tape with a polynomial regression equation, Y=(-6288,6)+(1378.8X)-(113.0X2)+(4.1X3)-(0.056X4), which has a deviation 0.5%, and 7.4% using Calfscale BW Tape. Conclusion of this research is that predicting birth weight of dairy cow’s calves using regular measuring tape is more accurate than using Calfscale BW Tape
Evaluation of neck blast resistance and agronomical performances on double haploid rice population in greenhouse and endemic field GUT WINDARSIH; DWINITA WIKAN UTAMI
Nusantara Bioscience Vol. 9 No. 4 (2017)
Publisher : Smujo International

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/nusbiosci/n090406

Abstract

Windarsih G, Utami DW. 2017. Evaluation of neck blast resistance and agronomical performances on double haploid rice population in greenhouse and endemic field. Nusantara Bioscience 9: 371-377. Blast disease caused by fungal Pyricularia grisea Sacc. is one of the most destructive diseases of rice in the world. The development of blast-resistant rice varieties will be essential to control this disease. This research aimed (i) to compare the resistance response to neck-blast among DH lines from double cross IR54/Parekaligolara//Bio110/Markuti and the differential varieties against three selected Indonesian blast races in greenhouse, (ii) to identify the gene(s) that caused the resistance to neck-blast based on the association between the resistance response and the genotype evaluation using molecular markers linked to Pi1, Pi33, Pib, Pir4 and Pir7 genes, and (iii) to evaluate the resistance response to leaf and neck blast on DH lines in endemic field (Sukabumi) and the agronomical performance of selected DH lines in optimum field in Ciasem of Subang, West Java, Indonesia during December 2013 to March 2014. Eleven double haploid lines from double-crossing IR54/Parekaligolara//Bio110/Markuti, the differential varieties as resistant control and the US2 variety for susceptible control were observed for neck-blast resistance response to three blast races in greenhouse and endemic field (Sukabumi), while the agronomical performances were observed in field of Ciasem-Subang. The results based on the genotyping evaluation, leaf and neck blast resistance, either in greenhouse and endemic location, and the agronomical performance in field showed that 5 selected double haploid lines had leaf and neck blast resistance and good performance on field trial. Thus they are promising for use either for further testing forwarding into releasing variety or used as donor for further blast resistant breeding activities.