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Nusantara Bioscience
Published by Universitas Sebelas Maret
ISSN : 20873948     EISSN : 20873956     DOI : https://doi.org/10.13057/nusbiosci
Core Subject : Education,
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Aims and Scope Nusantara Bioscience (Nusantara Biosci) encourages submission of manuscripts dealing with all aspects of biological sciences that emphasize issues germane to biological and nature conservation.
Arjuna Subject : Umum - Umum
Articles 545 Documents
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Impact of cognitive-behavioral therapy on daily living skills of high functioning autistic children with anxiety disorders Atallah Alenezi; Khadiga Abd-Elgied Gomea Gomea Hassan; Tahany El-Sayed El-Sayed Amr; Abdulellah Alsolais
Nusantara Bioscience Vol. 13 No. 1 (2021)
Publisher : Smujo International

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/nusbiosci/n130106

Abstract

Abstract. Alenezi AK, Hassan KAG, Amr TEE, Alsolais A. 2021. Impact of cognitive-behavioral therapy on daily living skills of high functioning autistic children with anxiety disorders. Nusantara Bioscience 13: 41-46. Cognitively Behavioral Therapy (CBT) is a psychotherapeutic intervention that is used to improve mental health. It is known to have positive effect on the daily living skills of autistic children with anxiety disorders. The aim of the current study was to evaluate the effect of cognitive-behavioral therapy on daily living skills of high functioning autistic children with anxiety disorders. A Quasi-experimental research design was adopted in this research. A purposive sample of 100 autistic children (50 in intervention group and 50 in control group) aged between 7-8 years were included in this study. Three main tools were used: Vineland Adaptive Behavior Scale used to measure the everyday living skills of children, Parent-Child Interaction Questionnaire (PACHIQ), and Diagnostic and Statistical Manual of Mental Disorders (DSM-V) for assessing anxiety disorder in children and adolescents 6-18 years. The results showed an improvement in children’s daily life skills and slight reductions in caregiver’s participation in the daily life skills of children. There was a significant difference between intervention group and control groups (p < 0.001). The current results exhibit that CBT may help in increasing autistic children's independence towards daily living skills.
Organic fertilizer applications improve the growth of vegetable crops and chemical properties in the tailings deposition area at Timika, Papua, Indonesia SARTJI Taberima; Saraswati Prabawardani; ROBERTH SARWOM; Graham Lyons
Nusantara Bioscience Vol. 12 No. 2 (2020)
Publisher : Smujo International

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/nusbiosci/n120208

Abstract

Abstract. Taberima S, Prabawardani S, Sarwom R, Lyons G. 2020. Organic fertilizer applications improve the growth of vegetable crops and chemical properties in the tailings deposition area at Timika, Papua, Indonesia. Nusantara Bioscience 12: 134-142. This study aimed to observe growth responses of selected vegetable crops grown in the media of inactive tailings, the available nutrients (macro and microelements) in fresh tailings before and after planting, as well as the metal uptake by plant tissues. Research was conducted using Randomized Block Design with three treatments of organic matter (chicken manure, cow manure, and compost of Salvinia natans) to observe the response on growth and yield of kangkong, red spinach, tomato, and chili. Based on the geochemical data, the ratio of ANC/MPA ranged from 3.81 (0-20 cm) to 4.60 (20-40 cm). There was no acid potential, with ANC ratio > 1.5 x MPA, and this result was supported by soil pH, which was alkaline in both depths of tailings media, ranging from 7.94 (0-20 cm) to 7.90 (20-40 cm). There was no significant effect on the chemical properties in the first period before and after planting for three months of planting period, except for the application with organic fertilizers. The best organic fertilizers applied in fresh tailings were chicken manure, followed by the compost of S. natans and cow manure. These organic fertilizers produced good growth and yield components of kangkong, red spinach, tomato, and chili. Meanwhile, the lowest growth and yield response of these crops was shown by control (without compost or manure). The level of metal uptake by all vegetables was low, suggesting that these vegetables are safe to be consumed.
Isolation and enumeration of bacteria responsible for nosocomial infections from houseflies and determining their susceptibility to poison bait azam haeidari; DAVOOD KESHAVARZI; Parviz Owlia; Hassan Vatandoost; ATIYEH RAFINEJAD; Rafinejad Javad
Nusantara Bioscience Vol. 13 No. 1 (2021)
Publisher : Smujo International

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/nusbiosci/n130104

Abstract

Abstract. Haeidari A, Keshavarzi D, Owlia P, Vatandoost H, Rafinejad A, Rafinejad J. 2021. Isolation and enumeration of bacteria responsible for nosocomial infections from houseflies and determining their susceptibility to poison bait. Nusantara Bioscience 13: 24-28. Nosocomial infections represent a serious public health concern in developing countries. Houseflies are one of the most common household pests carrying different pathogenic organisms. The purpose of this study was to isolate and enumerate bacteria species from house flies and to determine their susceptibility to Agita® fly bait. Flies were collected from two hospital environments between July to December 2014, in Yazd Province of Iran. Bacterial species were isolated from the outer surfaces of flies, and Agita® efficacy was evaluated based on lethal time (LT50) after 1, 2, 4, 8, 16, and 32 minutes. Three species of bacteria (Escherichia coli, Pseudomonas aeruginosa, and Staphylococcus aureus) responsible for nosocomial infections have been isolated and enumerated from flies. Among the 30 flies collected, 17, 24, and 3 flies were contaminated with E. coli, S. aureus, and Ps. aeruginosa, respectively. The minimum number of isolated bacteria was 3 × 102 CFU/mL, whereas the highest number was 2.4 × 105 CFU/mL. The susceptibility results showed that, despite the existence of a significant effect for heterogeneity in both field and laboratory strains (p-value ? 0/05) and an increase in the mortality of houseflies during the time, there was no significant difference between two strains regarding the efficacy of Agita® against houseflies. The findings of the present study revealed and confirmed that houseflies have an important role in the spread of nosocomial infections in hospital environments, and they are susceptible to Agita® fly bait.
Short Communication: Serratia rubidaea as contaminant in laboratory environment Dewi Peti Virgianti
Nusantara Bioscience Vol. 13 No. 1 (2021)
Publisher : Smujo International

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/nusbiosci/n130107

Abstract

Abstract. Virgianti DP. 2021. Short Communication: Serratia rubidaea as contaminant in laboratory environment. Nusantara Bioscience 13: 47-51. There have been many cases of bacterial contamination in the laboratory. The bacterial genera identified as contaminants are Bacillus, Staphylococcus, Micrococcus, Pseudomonas, Shigella and Serratia. These bacteria are classified as non-pathogenic and pathogenic bacteria that can interfere with the test and potentially develop false-positive results. The present research has shown that red-colored contaminant bacteria develop in unused sterile media in our laboratory. Based on related information, Serratia marcescens is a red bacterial species that have been reported as a contaminant in the laboratory. The purpose of this study was to identifiy contaminant bacteria at the molecular level. Based on the phylogenetic characterization using the 16S rDNA gene region, this red contaminant bacterium was identified as Serratia rubidaea.
Potential of Candida glabrata from ragi as a bioethanol producer using selected carbohydrate substrates Micky Vincent; Queentety Johnny; Dayang Salwani Awang Adeni; Nurashikin Suhaili
Nusantara Bioscience Vol. 13 No. 1 (2021)
Publisher : Smujo International

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/nusbiosci/n130101

Abstract

Abstract. Vincent M, Johnny Q, Adeni DSA, Suhaili N. 2021. Potential of Candida glabrata from ragi as a bioethanol producer using selected carbohydrate substrates. Nusantara Bioscience 13: 1-10. The flexibility and efficiency of fermenting microorganisms to convert substrates to ethanol are important factors in achieving high bioethanol yields during ethanolic fermentation. In this study, Candida glabrata, a common yeast found in fermented food, was evaluated in terms of its capability to produce ethanol using different types of carbohydrates, which included simple saccharides (glucose, maltose, sucrose), polysaccharides (starch and cellulose) and complex carbohydrates (total sago effluent, TSE). Our results indicated that C. glabrata was able to efficiently produce ethanol from glucose at 79.84% TEY (Theoretical Ethanol Yield). The ethanol production from sucrose was low, which was only 6.44% TEY, while no ethanol was produced from maltose. Meanwhile, for complex carbohydrate substrates such as starch and cellulose, ethanol was produced only when supplementary enzymes were introduced. Simultaneous Saccharification and Fermentation (SSF) of starch dosed with amylases resulted in an ethanol yield of 55.08% TEY, whilst SSF of cellulose dosed with cellulases yielded a TEY of 31.41%. When SSF was performed on TSE dosed with amylases and cellulases, the highest ethanol production was recorded within 24 h, with a yield of 23.36% TEY. Lactic acid and acetic acid were found to be at minimal levels throughout the fermentation period, indicating an efficient ethanol conversion. A notable increase in C. glabrata biomass was observed in cultures fed with glucose, starch (with supplementary amylases), and TSE (with supplementary amylases and cellulases). The current study indicates that C. glabrata can be used for bioethanol production from glucose, polysaccharides, and complex starchy lignocellulosic substrates such as TSE via SSF.
Human myiasis survey in Ilam Province, Southwest of Iran Morteza Akbari; Javad Rafinejad; AHMAD-ALI HANAFI-BOJD; Ali Ashraf Aivazi; Akbar Biglarian; SORAYA SHEIKHI; ZAHRA SHAVALI; KAMRAN AKBARZADEH
Nusantara Bioscience Vol. 12 No. 2 (2020)
Publisher : Smujo International

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/nusbiosci/n120209

Abstract

Abstract. Akbari M, Rafinejad J, Hanafi-Bojd AA, Aivazi AA, Biglarian A, Sheikhi S, Shavali Z, Akbarzadeh K. 2020. Human myiasis survey in Ilam Province, Southwest of Iran. Nusantara Bioscience 12: 143-147. Myiasis is the infestation of live human and vertebrate animals with dipterous larvae, which at least for a period. Ilam Province of Iran is one of the most important animal husbandry areas, especially nomadic, in Iran. The objective of this study was to identify the prevalence of myiasis in shepherds in Ilam Province. A cross-sectional study was conducted among the shepherds in Ilam Province, western Iran. Out of the 11 counties in Ilam Province, 6 were randomly chosen from three different climates for this study. A questionnaire was used by a trained interviewer to obtain the information from subjects. The disease has been seen in spring, summer, and autumn seasons. About 94.1% of people have been infested at least once. Pharyngeal myiasis had the highest prevalence with 58.3%. Itchy, painful throat, sneeze, cough, and headache were common symptoms. About 85.1% of people described the symptoms of the disease as severe and very severe. About 75.4% stated that the duration of the disease was more than 5 days. According to the results of the study, it was found that the prevalence of myiasis among shepherds in the Ilam Province is high and it is necessary to take appropriate measures to control the disease and increase health literacy.
Histological aberrations and mode of damage of cowpea (Vigna unguiculata) by Colletotrichum destructivum David Nwazuo Enyiukwu; ANDERSON CHIDI AMADIOHA; CHARLES CHIMEZIE ONONUJU
Nusantara Bioscience Vol. 13 No. 1 (2021)
Publisher : Smujo International

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/nusbiosci/n130103

Abstract

Abstract. Enyiukwu DN, Amadioha AC, Ononuju CC. 2021. Histological aberrations and mode of damage of cowpea (Vigna unguiculata) by Colletotrichum destructivum. Nusantara Bioscience 13: 16-23. Leaf and stem of healthy 2-week old cowpea (Vigna unguiculata L. Walp.) seedlings were inoculated with spore suspension of the Colletotrichum destructivum O’Gara. Sections of the infection courts were prepared and examined under digital microscope. The results showed that the infection process began 12 hours after inoculation (hai) with the germination of spores of the fungus. Large multi-lobed primary hypha (somewhat spherical in shape) developed from the infection peg and remained inside a single epithelial cell for about 3 days. Towards the end of this time, the primary hyphae developed thin filamentous tentacles that punctured and branched into adjoining cells, initiating necrotrophic phase of the disease. During this period, typical anthracnose symptoms began to develop on the infected organs of the crop. The entire infection process lasted a maximum of 96 h, at the end of which acervulus that bore a single seta emerged on infected crop lesions. The mechanism of damage of the pathogen involved intra-cellular and inter-cellular colonization of the host tissues early and late in the disease cycle. The integrity of the affected tissues' cells was compromised by passage and colonization of Colletotrichum destructivum O’Gara evidenced by lack of clear-cut middle lamella and cell boundaries. Anthracnose affected cells dispossessed of vital nutrients became turbid, devoid of turgidity and vigor. Thus, these results have strong implications for plant health management in that effective environment-compliant control of the fungus should be initiated on or before the third day following arrival of the conidia of the pathogen on the crop. Seeds are major agents of introduction of Colletotrichum spp. to disease-free locations. Findings from this study also support that strong trans-border control of seeds of the crop should be maintained since the pathogen is known to be seed-borne and demonstrates sufficient cross-infection of crop plants. Key words: Hemibiotrophy,  Colletotrichum destructivum, Anthracnose, Cowpea,  Hyphal colonization
Golden apple snail (Pomacea canaliculata) as an alternative protein source in Pasupati catfish (Pangasius sp.) fish feed Meilisha Putri Pertiwi; Dina Dyah Saputri
Nusantara Bioscience Vol. 12 No. 2 (2020)
Publisher : Smujo International

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/nusbiosci/n120212

Abstract

Abstract. Pertiwi MP, Saputri DD. 2020. Golden apple snail (Pomacea canaliculata) as an alternative protein source in Pasupati catfish (Pangasius sp.) fish feed. Nusantara Bioscience 12: 162-167. Research on efforts to conserve golden apple snail (Pomacea canaliculata) meat has been done. This research aimed to utilize the snail meat as an alternative feed for the Pasupati catfish (Pangasius sp.). The research was carried out from May to August 2020. It began by collecting Pomacea canaliculata by hand sorting then processed into artificial feed/pellets. The research was CRD designed with 5 treatments and 3 replications. Pasupati catfish juvenil measuring 7.4 ± 0.8 cm and weighing 11.7 ± 0.5 g, was maintained for 21 days with a stocking density of 15 individuals/aquarium. Feeding as much as 8% of the biomass with a frequency of feeding 3 times a day at satiation. Variables measured were SGR, L, RP, FCR, and SR. The results showed that feed B produced the best fish growth and could be an alternative fish feed without having a negative effect on growth and feed utilization. Feed B (90% fish meal + 10% Pomacea canaliculata meat meal) had the highest SGR value and was supported by a high PR value and a low FCR value. In addition, there is a difference among variables, despite least significant. ANOVA test also supports the differences between treatments, but HSD test (p<0,05) is not significantly different. This means that the addition of the percentage of P. canaliculata meat meal to the feed does not provide a good performance of the Pasupati catfish. Value of abiotic parameters was ??to support fish farming.
Effectiveness of maggot extractions and secretion (E/S) of Lucilia sericata in reducing wound surface in experimental scalding burn injury Zahra Sadat Amiri; Kamran Akbarzadeh; Masoumeh Douraghi; Khosrou M Abdi; Abbas Aghaei Afshar; jasem ghaffari; Razieh shabani Kordshouli; Morteza Akbari; Javad Rafinejad
Nusantara Bioscience Vol. 13 No. 1 (2021)
Publisher : Smujo International

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/nusbiosci/n130102

Abstract

Abstract. Amiri ZS, Akbarzadeh K, Douraghi M, Abdi KM, Afshar AA, Ghaffari J, Kordshouli RS, Akbari M, Rafinejad J. 2021. Effectiveness of maggot extractions and secretion (E/S) of Lucilia sericata in reducing wound surface in an experimental scalding burn injury. Nusantara Bioscience 13: 11-15. Lucilia sericata larvae have been successfully used as medicinal larvae for wound healing. This study was performed to evaluate the therapeutic effects of the primary ointment made from excretory-secretory substances of L.sericata larvae on experimental burn wounds on rabbits under laboratory conditions. Extraction of ES from the third stage of L.sericata larvae and antibiogram testing was done. Four rabbits were randomly divided into four groups; three standard third-grade burn wounds were created on the back of each rabbit for intervention groups (B, D) of wounds were used respectively from the original ointment made from ES and ES without accompanying material. Nitrofurazone ointment was used for the positive (C) and placebo ointment was used for the negative (A). On the 21st day, all scars of wounds in groups B and D were separated and the wound was free of infectious tissue, whereas in groups A and C that the scars were clinging to the base. The level of the ulcer was significantly lower in the E/S intervention group and the infectious tissue was not observed during the treatment of group B and D wounds. Methicillin-resistant Staphylococcus aureus (MRSA) did not show resistance to excretory-secretory substances of L. sericata larvae. The debridement and recovery process was faster in the ES-treated group and the initial ointment than in the control group. The results showed that maggot extractions and secretion (E/S) of L. sericata in reducing wound healing is appropriate for treatment.
Evaluation of fluctuating asymmetry and sexual dimorphism of Channa striata using landmark-based geometric morphometric analysis from Agusan Marsh and Lake Mainit in Caraga Region, Philippines Rex Bomvet Saura; Geneveve Falcasantos; Rex Jr. Andante; Luna Munda; Marycris Alimorong; Brent Joy Hernando
Nusantara Bioscience Vol. 13 No. 1 (2021)
Publisher : Smujo International

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/nusbiosci/n130114

Abstract

Abstract. Saura EBD, Falcasantos GC, Andante RJM, Munda LC, Alimorong MM, Hernando BJ. 2021. Evaluation of fluctuating asymmetry and sexual dimorphism of Channa striata using landmark-based geometric morphometric analysis from Agusan Marsh and Lake Mainit in Caraga Region, Philippines. Nusantara Bioscience 13: 100-110. Evaluation was conducted to determine the impact of ecological condition to Channa striata using fluctuating asymmetry (FA) employing the landmark-based geometric morphometric analysis, using TPS software, SAGE, PAST-Hotellings Test, including Physico-chemical analysis, cyanide and four base metals. Results of Physico-chemical analysis of the two habitats showed Agusan marsh with low oxygen level (3.37-4.55) and high in Iron and TDS as compared to DENR Standards. The statistical results showed high variations (p < 0.0001) on left and right-side having percentage of 79.22% in Agusan Marsh and 72.35% in Lake Mainit which is relatively high Fluctuating Asymmetry. In terms of sex, Agusan marsh had 84.27% and 85.41% FA in males and females while Lake Mainit having 85.12% and 80.89% in males and females. The fairly similar percentage of Fluctuating Asymmetry confirms no sexual dimorphism and no shape differences between male and female, and no peculiar characters to distinguished between sexes based on the 17 landmark points. Present study concluded that FA observed in the snout tip was due to breeding behavior, the landmark points in cephalic, pectoral, and caudal region were associated with burrowing and mobility factors within the lateral line system in these fishes which thrive in high turbid habitat and low oxygen. Furthermore, it was found that the level of FA tends to increase in disturbed environs.

Page 38 of 55 | Total Record : 545

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