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Microbiology Indonesia

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Published by Perhimpunan Mikrobiologi Indonesia

ISSN : 19783477 EISSN : 20878575 DOI : -

Core Subject : Health, Science,

Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology

Microbiology Indonesia provides a unique venue for publishing original researches in microbiology (espesially from Indonesian reseachers), and ensures that authors could reach the widest possible audience. Microbiology Indonesia publishes a wide range of research disciplines on bacteria, archaea, fungi, protozoa, and virus as well as biotechnology related to microbiology. Topics include (but are not limited to): -methods in microbiology, -bioprocess, -environmental microbiology, -food microbiology, -plant-microbe interaction, -animal-microbe interactions, -microbial community, -microbial genetics, -virology, -comparative and functional microbial genomics, -and gene expression in microbes.

Arjuna Subject : Imunologi dan Mikrobiologi (semua kategori) - Mikrobiologi dan Bioteknologi Terapan

Articles 398 Documents

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Phenolic Compounds of Sponge-associated Fungi (Lecanicillium evansii) HEFNI EFFENDI
Microbiology Indonesia Vol. 6 No. 3 (2012): September 2012
Publisher : Indonesian Society for microbiology

This study was chiefly aimed at pursuing new biologically active secondary metabolites of microfungus species, Lecanicillium evansii, isolated from sponge Callyspongia sp collected from West Bali Sea, Indonesia. Sponges were collected by scuba diving. A tiny piece of sponge was inoculated on the surface of malt agar plates and incubated at 27 ºC. In order to get a pure mono-culture of the fungus, repeated sub-culturing onto fresh malt agar plates were performed. The collected fungi were maintained on malt agar plates using the Wickerham medium. Mass cultivation of the fungus L. evansii (10 L) was carried out in 30 erlenmeyer flasks in Wickerham medium. After 10 days incubation, without shaking under constant room temperature (20 ºC), fungal mycelium were separated from the culture broth. The mycelia were extracted with methanol and ethyl acetate was added to the media. Both methanol-added mycelia and ethyl acetate-added media were left overnight. Seven compounds were isolated from L. evansii. Those compounds comprised phenolic compounds (terphenylin, deoxyterphenylin, terprenin 2, terprenin epoxide), bipeptide (cyclo-tyrosylprolyl), and simple aromatic compounds (acetyl hydroxybenzamide, 4-hydroxybenzaldehyde). Detailed analysis by NMR and mass spectrometry enabled their identification to be new deoxyterphenylin, new terprenin 2, and new terprenin epoxide.

Enzymatic Characterization of Recombinant Cyclodextrin Glycosyltransferase from Bacillus sp. a2-5a using Sagoo Starch as Substrate RINA IMANIAR; CATUR RIANI; DESSY NATALIA; DEBBIE SOFFIE RETNONINGRUM
Microbiology Indonesia Vol. 6 No. 3 (2012): September 2012
Publisher : Indonesian Society for microbiology

Cyclodextrin (CD) is a cyclic oligosaccharide molecule and depending on the number of glucose molecules, three types of CDs are commonly used, α-CD, β-CD, and γ-CD. CDs can be produced enzymatically using starch as substrate catalyzed by CD glycosyltransferase (CGTase). In current research, recombinant CGTase production from the synthetic gene was optimized for its production using three growth media and two induction temperatures. The highest yield was obtained in Luria Bertani medium at 25 °C. The rCGTase protein was affinity purified as a 76.39 kDa protein which showed α-cyclization and starch hydrolysis activities using zymography method. The optimum temperature, pH and incubation time was 55 °C, 6, and 24 h, respectively. The enzyme was stable at a wide pHs in the range of 5-10, retained its half activity at 56 °C for 30 min and had cyclization ratio for α-CD : β-CD : γ-CD was 4 : 81 : 15. An amount of 542 mg β-cyclodextrin was produced from 100 mL reaction of 1% (b/v) sagoo starch using 38.4 μg rCGTase in optimum condition. This work reports for the first time the character of rCGTase from Bacillus sp. A2-5a using sagoo starch as a substrate.

Molecular and Physiological Characterization of Copper-Resistant Bacteria Isolated from Activated Sludge in an Industrial Wastewater Treatment Plant in Rungkut-Surabaya, Indonesia WAHYU IRAWATI; TRIWIBOWO YUWONO; JOEDORO SOEDARSONO; HARI HARTIKO
Microbiology Indonesia Vol. 6 No. 3 (2012): September 2012
Publisher : Indonesian Society for microbiology

Copper resistant bacteria can be isolated from environments where copper levels are abundant from mining, industrial, or agricultural activities. The aim of this work was to study the molecular and physiologicalcharacteristics of indigenous copper resistant bacteria isolated from activated sludge in an industrial wastewatertreatment plant in Surabaya, Indonesia. The bacterial isolates were designated as strains IrC1, IrC2, and IrC4. Phylogenetic analysis based on 16S rDNA sequence analysis identified isolates IrC1, IrC2, and IrC4 as Acinetobacter oleivorans (98.41% similarity), Acinetobacter pitii (97.22% similarity), and Cupriavidus pauculus (96.99 similarity), respectively. The addition of 5 mM of CuSO4 in the medium affected morphological 4 appearance of all isolates to green and undulate margin might be due to the survival mechanism of bacteria by absorbing the copper. This studies indicated that copper resistance mechanism of all isolates was facilitated through the bioaccumulation of copper inside the cell, especially on the membrane fraction and inside the cytoplasm, albeit at a limited amount. It was observed that isolates IrC1, IrC2, and IrC4 were capable of accumulating 137.23 , 364.66 , and 272.07 mg L-1 of copper, respectively from the medium containing 8 mM CuSO4. The capability of isolates IrC1, IrC2, and IrC4 to accumulate copper can be exploited in bioremediation 4 process for removing copper from industrial sewage.

Effects of Tetracycline and Temperature on Drosophila melanogaster Infected with Wolbachia Inducing the Popcorn-Effect ENDANG SRIMURNI KUSMINTARSIH
Microbiology Indonesia Vol. 6 No. 3 (2012): September 2012
Publisher : Indonesian Society for microbiology

The expression of cytoplasmic incompatibility, parthenogenesis, and feminization in many hosts of Wolbachia is influenced by environmental factors such as temperature and antibiotics. Therefore, it might also affect Wolbachia inducing popcorn-effect. To examine the effects of temperature and antibiotic treatment on the life span of Drosophila melanogaster harbouring the popcorn-effect inducing strain of Wolbachia, flies were reared in different temperature such as at 20 and 29 °C, and cultured (from egg to adult stage) on a medium containing tetracycline. The tetracycline-treated Wolbachia was established by placing 0.25 mg m L-1 of tetracycline in the media. The result showed that there was no difference in the life span of D. melanogaster infected with Wolbachia popcorn-effect under untreated and treated condition with tetracycline at 20 °C. Therefore, there is no popcorn-effect in the D. melanogaster at low temperature (20 °C). While the life span of D. melanogaster at 29 °C, where infected flies have a shorter life span than treated flies. Popcorn-effect (shorter life span) was found at 29 °C.

Decolorization of Orange II by Mixed Culture of Enterococcus faecalis ID6017 and Chryseobacterium indologenes ID6016 VINCENTIA IRENE MEITINIARTI; KRIS HERAWAN TIMOTIUS; ENDANG SUTARININGSIH SOETARTO; EKO SUGIHARTO
Microbiology Indonesia Vol. 6 No. 3 (2012): September 2012
Publisher : Indonesian Society for microbiology

Previous work showed that Enterococcus faecalis ID6017 and Chryseobacterium indologenes ID6016 were able to decolorize the orange II qualitatively. In that experiment, E. faecalis could decolorize orange II more rapidly than C. indologenes. The objective of this study was to examine the decolorization of orange II by mixed culture and the growth of both bacterial species on orange II containing medium. The experiment was done in 500 mL sterilized Erlenmeyer flasks containing 285 mL growth media with 80 mg L-1 orange II. Five different treatments were performed in this project, i.e. medium was inoculated with (i) 15 mL of sterile aquadest, (ii) 15 mL of C. indologenes, (iii) 15 mL of E. faecalis, (iv) 7.5 mL of C. indologenes and 7.5 mL of E. faecalis, and (v) 7.5 mL of E. faecalis until decolorization occured , followed by inoculation with 7.5 mL of C. indologenes. Bacterial growth (total cells number), orange II, glucose, and suphanilic acid, as intermediate product of orange II decolorization, concentrations were measured every 2 h.The maximum decolorization of orange II was observed in the medium inoculated with a mixed culture of E. faecalis and C. indologenes. Decolorization of orange II occurred of growth and gave final concentration of sulphanilic acid of 7.06 mg L-1. During culture both species grow in equilibrium in terms of population.

Profile of Mutation on Drug Resistance Mycobacterium leprae Isolates in Indonesia Collected During 2003-2011 RATNA WAHYUNI; DINAR ADRIATY; ISWAHYUDI ISWAHYUDI; CITA ROSITA SIGIT PRAKOESWA; INDROPO AGUSNI; SHINZO IZUMI
Microbiology Indonesia Vol. 6 No. 3 (2012): September 2012
Publisher : Indonesian Society for microbiology

Multidrug therapy (MDT) regiment has been used for leprosy all over the world for more than 20 years. Drug resistances of Mycobacterium have been reported from many areas. The resistance mostly occurred due to mutation on the gene coding protein targeted by anti-leprosy drugs. Two hundreds and seventy M. leprae isolates from some area in Indonesia were examined for studying the profile of mutation among isolates collected during 2003-2011. Drug resistance determining region of the folP1 gene and the rpoB gene was sequenced. The results showed 5 isolates of M. leprae harboured mutation only in the folP1 gene and another isolate harbored mutation in both the folP1 and rpoB gene. The point mutation in the folP1 gene that was found in 2 isolates occurred in codon 53 (ACC→GCC; Thr→Ala). Double point mutations on codon 53 that was found in two isolates were ACC→AGA (Thr→Arg) and ACC→AGG (Thr→Arg). The point mutation in the folP1 geneoccurred in codon 55 were found in two isolates were CCC→CTC (Pro→Leu) and CCC→CGC (Pro→Arg). Whereas mutation in the rpoB gene in one isolate occurred in codon 410 was GAT→TAT (Asp→Tyr). These mutations that altered the amino acids of the protein revealed that isolates of M. leprae were resistant to drug with variable profiles

Bacterial Response after Exposure with Pure Metabolite Produced by Streptomyces sp. BL225 Isolated from Batanta Island's Leaf Litter ARIF NURKANTO; ANDRIA AGUSTA; WELLYZAR SJAMSURIDZAL; HEDDY JULISTIONO
Microbiology Indonesia Vol. 7 No. 1 (2013): March 2013
Publisher : Indonesian Society for microbiology

The objective of this research was to investigate bacterial response after treatment with active metabolite produced by Streptomyces sp. isolated from Batanta Island. Minimum inhibitory concentration (MIC) values of four clinically tested bacteria (Escherichia coli, Bacillus subtilis, Staphylococcus aureus, and Micrococcus luteus) were successfully determined in this research using microdilution method. Leakages of nucleic acids and proteins from the tested microbes were detected using UV/VIS spectrophotometry method at 260 and 280 nm. Uracil leakage was analyzed using HPLC. Morphological changes of the bacterial cells were observed using scanning electron microscope (SEM). A Streptomyces isolate BL225 was identified based on the 16S rRNA gene sequence (1500 bp). When tested agains microbes, the MICs values of this compound were between 16-64 µg mL-1. The results indicated leakages of protein, nucleic acid and uracil from E. coli and B. subtilis cells after treatment with pure metabolite isolated from BL225. Treatment using metabolite from BL225 also caused morphological changes and damages of the target bacterial cell. BL225 had been identified as a strain that has closed relation to Streptomyces badius (98.9%).

Cloning and Expression of Serotype-2 Simian Betaretrovirus Reverse Transcriptase Gene Isolated from Indonesian Cynomolgus Monkey in Escherichia coli UUS SAEPULOH; DIAH ISKANDRIATI; FUNGKEY HOETAMA; SELA SEPTIMA MARIYA; DEDY DURYADI SOLIHIN; JOKO PAMUNGKAS; DONDIN SAJUTHI
Microbiology Indonesia Vol. 7 No. 2 (2013): June 2013
Publisher : Indonesian Society for microbiology

In this study, we isolated the simian betaretrovirus serotype-2 (SRV-2) reverse transcriptase (RT) gene from infected Indonesian cynomolgus monkey (Macaca fascicularis). The gene was then cloned in Escherichia coli expression system. The SRV-2 RT gene is located between nucleotides 3284-4925 in the polyprotein (Pol) region encodes 547 amino acids. Analysis of expression using SDS-PAGE and western blot techniques showed a specific band of 64.9 kDa, indicating SRV-2 RT recombinant enzyme. Purification of SRV-2 RT recombinant enzyme produced 312 μg mL-1 protein with 7.1 U μL-1 enzyme activities. Application of this recombinant enzyme in reverse transcription-PCR (RT-PCR) of β-globin and β-actin genes produced DNA fragments of 206 and 350 bp, indicating amplification of β-globin and β-actin genes, respectively. Therefore, the expressed SRV-2 RT enzyme was proven to be functional, although the activity was low.

The Effect of a Mixed-Starter Culture of Lactic Acid Bacteria on the Characteristics of Pickled Orange-Fleshed Sweet Potato (Ipomoea batatas L.) NETI YULIANA; SITI NURDJANAH; MIKA MARGARETA
Microbiology Indonesia Vol. 7 No. 1 (2013): March 2013
Publisher : Indonesian Society for microbiology

In this study, fermentation process was carried out on orange-fleshed sweet potato cubes to produce sweet potato pickle using a mixed culture of Lactobacillus plantarum and Leuconostoc mesenteroides at 30 °C over 12 days period. Spontaneous fermentation was also performed as a control. Samples were withdrawn at various time intervals for analyses of reducing sugar content, total number of lactic acid and non-lactic acid bacteria, lactic acid concentration, pH, and sensory attributes. The results showed that using a mixed culture of L. plantarum and L. mesenteroides could greatly reduce contamination of non-lactic acid bacteria, retaining low amount of reducing sugar, rapidly producing lactic acid and consequently decreasing pH value of the pickle, as well as giving better sensory score. After 12 d of fermentation, sample of pickle inoculated with mixed culture showed the following characters: total lactic acid content 0.5%, total lactic acid bacteria 8.46 log10 CFU mL-1, total non-lactic acid bacteria 1 log10 CFU mL-1, total reducing sugar 0.84 g L-1, texture 64.92 mm 50 g-1 s-1, and hedonic sensory score for both taste and aroma 4 (like) in a scale of 5. These results indicated the potential ability of the mixed culture of lactic acid bacteria to improve the quality of the pickle fermented spontaneously.

Optimization Modeling of Ethanol Production from Shorgum bicolor grain: Comparison between Separate Hydrolysis Fermentation and Simultaneous Saccharification Fermentation RUTH CHRISNASARI; DAMIATI HARTINI SUSETYO; ADRIAN PRATAMA SUGIANTO; TJANDRA PANTJAJANI
Microbiology Indonesia Vol. 7 No. 1 (2013): March 2013
Publisher : Indonesian Society for microbiology

Two different process configurations, simultaneous saccharification and fermentation (SSF), and separate hydrolysis and fermentation (SHF), were compared for ethanol production from Shorgum bicolor grain. Optimization modeling for glucoamylase and Zymomonas mobilis concentration in both of SSF and SHF were carried out to obtain optimal concentration of ethanol production. The optimum condition was achieved using 0.021 % (v/v) of glucoamylase, and 30.19% (v/v) of Z. mobilis for SHF. In contrast, the optimum condition for SSF was 0.021 % (v/v) of glucoamylase and 17.51% (v/v) of Z. mobilis. The model predicted SHF processing to be superior. The superiority of SHF over SSF was confirmed experimentally, the result showed ethanol yield of SHF was 134.80 g L-1 and ethanol yield of SSF was 115.66 g L-1 after 72 h incubation time. A high similarity was observed between the predicted and experimental results, demonstrating the accuracy of the model.

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Home Page

OAI Link

Editorial Team

Contact

Reviewer

Google Scholar

Contact Name Iman Rusmana

Contact Email rusmana13@yahoo.com

Phone +62217560536

Journal Mail Official microbiology.indonesia@gmail.com

Editorial Address kPERHIMPUNAN MIKROBIOLOGI INDONESIA (SeKretariat PERMI), Gedung 10.2 Indonesian Life Sciences Center (ILSC), Zona Bisnis Teknologi Puspiptek, Jalan Raya Serpong - Bogor Gunung Sindur, Jawa Barat 16340, Indonesia. Email: microbiology.indonesia@gmail.com

Location Kota tangerang, Banten INDONESIA

Abstract

Abstract

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Abstract

Abstract

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Abstract

Contact Name
Iman Rusmana

Contact Email
rusmana13@yahoo.com

Phone
+62217560536

Journal Mail Official
microbiology.indonesia@gmail.com

Editorial Address
kPERHIMPUNAN MIKROBIOLOGI INDONESIA (SeKretariat PERMI), Gedung 10.2 Indonesian Life Sciences Center (ILSC), Zona Bisnis Teknologi Puspiptek, Jalan Raya Serpong - Bogor Gunung Sindur, Jawa Barat 16340, Indonesia. Email: microbiology.indonesia@gmail.com

Location
Kota tangerang,

Banten

INDONESIA