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Microbiology Indonesia

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Published by Perhimpunan Mikrobiologi Indonesia

ISSN : 19783477 EISSN : 20878575 DOI : -

Core Subject : Health, Science,

Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology

Microbiology Indonesia provides a unique venue for publishing original researches in microbiology (espesially from Indonesian reseachers), and ensures that authors could reach the widest possible audience. Microbiology Indonesia publishes a wide range of research disciplines on bacteria, archaea, fungi, protozoa, and virus as well as biotechnology related to microbiology. Topics include (but are not limited to): -methods in microbiology, -bioprocess, -environmental microbiology, -food microbiology, -plant-microbe interaction, -animal-microbe interactions, -microbial community, -microbial genetics, -virology, -comparative and functional microbial genomics, -and gene expression in microbes.

Arjuna Subject : Imunologi dan Mikrobiologi (semua kategori) - Mikrobiologi dan Bioteknologi Terapan

Articles 398 Documents

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16S rDNA Typing of Salmonella Typhi Strains from Different Geographical Locations in Sumba Island East Nusa Tenggara Indonesia
Microbiology Indonesia Vol. 7 No. 1 (2013): March 2013
Publisher : Indonesian Society for microbiology

A total of thirteen isolates representative of Salmonella Typhi from different geographical locations in Sumba Island, East Nusa Tenggara, Indonesia were identified by 16S rDNA gene sequences. Bacterial DNA extraction was prepared by using a PurelinkTM Genomic DNA kit. The bacterial DNA and control were amplified using the specific primers for S. Typhi. These 16S rDNA gene sequence data were aligned with the corresponding available S. Typhi sequence and the reference organisms from the family Enterobacteriaceae from NCBI database by using the CLUSTAL X software. Phylogenetic trees were generated using the PHYLIP software package and the matrix of nucleotide similarity and nucleotide difference were generated by using the PHYDIT software. The results from the 16S rDNA analysis showed that the degree of similarity within these strains ranged from 99.13-100%. The percentage of sequence similarity between S. Typhi strains was very high (>99 %). Molecular phylogenetic analysis showed that all of the isolates formed a new center of diversity with S. Typhi ATCC 19430T as a reference strain. Based on these results, all of the tested strains belonged to species of S. Typhi suggested by their relatedness with the type strain of S. Typhi ATCC 19430T.

Analysis of Bacterial Community Associated with Aaptos sp. from Rote and Seribu Islands EKOWATI CHASANAH; GINTUNG PATANTIS; ARIYANTI SUHITA DEWI; ENDAR MARRASKURANTO; HEDI INDRA JANUAR; STELLA STELLA; SUSAN SOKA; YOGIARA YOGIARA
Microbiology Indonesia Vol. 7 No. 1 (2013): March 2013
Publisher : Indonesian Society for microbiology

Aaptos sp. is a marine sponge that could produce bioactive compounds such as aaptamin, aaptosin, and isoaaptamin which have activities as antitumor, antimicrobial, and antiviral. Community of bacteria associated with the sponge might correlate with production of those bioactive compounds and be affected by water environment where the sponge grow. The presence of anthropogenic stressor such as pollutans might become a burden to the waters where the biota grown and could affect the microbial biodiversity in the sponge and its active metabolite produced. The objective of this research was to analyze bacterial community associated with Aaptos sp. from Rote Island and Seribu Islands, using T-RFLP method. The results showed that bacterial community associated with Aaptos sp. from both sampling sites shared 40.81% similarity in which they were dominated by the same bacteria class of Actinobacteria, Flavobacteria, α-proteobacteria, δ-proteobacteria, and γ–proteobacteria. The bacteria collected from Rote island were more highly distributed and diverse than those from Seribu Islands. A total of 23 classes of microorganism were identified in Rote Island waters, while in Seribu Islands was 14 classes of microorganism. The presence of Actinobacteria and Proteobacteria in Aaptos sp., is allegedly involved in the production of secondary metabolites.

Nitrous Oxide Reduction Activity of Denitrifying Ochrobactrum anthropi Isolated from Rice Field RATNA SETYANINGSIH; IMAN RUSMANA; PRIHASTO SETYANTO; ANTONIUS SUWANTO
Microbiology Indonesia Vol. 7 No. 2 (2013): June 2013
Publisher : Indonesian Society for microbiology

Nitrous oxide (N2O) is one of the principal greenhouse gases. Differences in soil microbial community composition affect N2O emission. Ochrobactrum anthropi BL1 and BLN1 isolated from rice field in Tangerang, Banten, Indonesia can grow on and reduce N2O. This study investigated the patterns of N2O reduction activity and growth of O. anthropi BL1 and BLN1 on denitrification media and also examined the ability of BLN1 strain to reduce N2O in flooded rice soil. Nitrous oxide reduction activity and growth of strains BL1 and BLN1 occurred simultaneously, indicating that the bacteria used N2O for growth. BL1 and BLN1 showed the same specific growth rate, but the N2O reduction rate of BLN1 was higher than that of BL1. Increase of the N2O concentration in the surface water of flooded soil without BLN1 isolate six hours after the addition of NO3- was significantly greater than the surface water from soil that had been inoculated with the isolate.

Mutagenic Improvement of Xylanase Production from Xylanolytic Bacteria and its Phylogenetic Analysis CHUSNUL HANIM; LIES MIRA YUSIATI; MUHAMMAD NUR CAHYANTO; ALI WIBOWO
Microbiology Indonesia Vol. 7 No. 2 (2013): June 2013
Publisher : Indonesian Society for microbiology

This study was conducted to obtain xylanolytic mutants that have higher xylanase activity than their wildtype counterparts. A mutant with the best xylanolytic activity was selected and identified based on its 16S rRNA sequence. Its optimum growth condition was also characterized and its phylogenetic relations to other xylanolytic bacteria were analzsed. Wild type xylanolytic alkalophlic bacteria were grown in medium containing xylan as a substrate. Mutation was performed using ethidium bromide (EtBr) or ethyl methanesulfonate (EMS) atconcentrations 50, 100, and 150 mg mL-1 and times of exposure 30, 60, 90, and 120 min for each treatment. Twenty two mutants were obtained from EtBr and 24 mutants from EMS mutageneses. The mutants were analyzed for their capability to secrete xylanase into xylan medium containing xylose or glucose or glycerol. Growth optimizations of the mutant were done in media with pH range 6-11 and temperature range 30 to 60 °C. Mutant number 19, which was obtained by treatment using 50 mg mL-1 EMS for 120 min, had the highest xylanase activity (15.057 U g-1). This activity was obtained at optimum growth conditions: pH 9.5 and temperature 55 °C. Chromosomal DNA of this mutant was extracted and amplified by PCR using 16S rRNA gene specific primers. The amplified fragments were sequenced by dideoxynucleotide chain terminator method. The phylogenetic analysis based on 16S rRNA gene sequence showed that mutant 19 was closed to an anaerobic xylanase producing bacteria.

Enzymatic and Acid Hydrolysis of Sago Starch for Preparation of Ethanol Production ROFIQ SUNARYANTO; BERTI HARIASIH HANDAYANI; RATU SAFITRI
Microbiology Indonesia Vol. 7 No. 2 (2013): June 2013
Publisher : Indonesian Society for microbiology

A series of studies on the hydrolysis of Sago starch for ethanol fermentation had been conducted. Hydrolysis of sago starch was carried out using sulfuric acid 2.5% and amylase(s) enzymes. The concentrations of sago starch used in this experiment were 5, 10, 15, 20, and 30% (w/v). The highest hydrolyzate containing reducing sugar was used as substrate for ethanol fermentation by Saccharomyces cerevisiae FNCC 3012. The results indicated that hydrolysis using 2.5% sulfuric acid for 120 min at 121 °C produced 6.6% (w/v) reducing sugar and hydrolysis using α-amylase and Dextrozyme DX produced more reducing sugar, 7% (w/v) and 17.1% (w/v), respectively. The fermentation of hydrolyzed sago starch by S. cerevisiae FNCC 3012 produced ethanol 7.98% (v/v).

Selection of Methods for Microbiological Extraction of Chitin from Shrimp Shells JUNIANTO JUNIANTO; BUDIASIH WAHYUNTARI; SISWA SETYAHADI
Microbiology Indonesia Vol. 7 No. 2 (2013): June 2013
Publisher : Indonesian Society for microbiology

Chitin extraction from shrimp shells involves two processing steps that are demineralization followed by deproteination process. Lactobacillus acidophilus FNCC 116 and Bacillus licheniformis F11.1 were used in demineralization and deproteination respectively. The overall objectives of this experiment were to determine fermentation systems which resulted in the highest mineral and protein removal. The demineralization experiments consisted of three different batch fermentation designs: batch fermentation (Am ); subsequent batch fermentation 1, in which 100% medium was replaced with fresh medium after 24 h fermentation (Bm ); and subsequent batch fermentation 2, in which 50% medium was replaced with the same amount of fresh medium after 24 h fermentation (Cm ). The demineralization was conducted at 30±2 °C, 50 rpm for 60 h. The deproteination experiments consisted of 3 different batch fermentation designs: batch fermentation 1, inoculum was added once at the beginning of the fermentation (A p); batch fermentation 2, inoculum was added twice, at the beginning and after 24 h fermentation (Bp ); and subsequent batch fermentation, 100% medium was replaced with fresh medium after 24 h fermentation (Cp ). The deproteination was carried out at 55 °C, pH 7.8-8.0, aeration 2.3 vvm, and agitation 275 rpm for 96 h. The experimental results showed that in the demineralization process, fermentation design B gave the highest ash removal. Ash removed in the fermentation design A , B , and C was 97.19, 99.69, and 97.69%, respectively. The protein removed in the fermentation design A , B , and C was 94.42, 94.51, and 95.37%, respectively.

Characterization of Micromonospora spp. with Activity Against E.coli ATCC 35218 Resistance β-Lactam Antibiotics DYAH NOOR HIDAYATI; YULIN LESTARI; BAMBANG MARWOTO
Microbiology Indonesia Vol. 7 No. 3 (2013): September 2013
Publisher : Indonesian Society for microbiology

The emerge of antibiotic resistance has been an important issue all over the world, on the other hand, infectious diseases have been one of the highest causes of death causes in the world. Therefore, the discovery of a new antimicrobial drug is very important, and the group of rare actinomycetes are really promising as the producer of new bioactive compounds, in this case antibiotics. In this study we screened and characterized the actinomycetes with antibacterial activity against Escherichia coli ATCC 35218 resistant beta-lactam antibiotics. A total of 96 strains collected in Biotechnology Microbial Culture Collection (BioMCC), BPPT, were screened for their antibacterial activities by the agar plug method. Three strains, at-HH-64, at-HH-78, and at-HH-259, showed antibacterial activity. The selected strains were cultured on four different media, both solid and liquid media, e.g. ISP2, ISP4, Micromonospora Starch Medium (MS), and Bennet’s Medium (BM), and we characterized their morphology and growth patterns. Morphological characterization showed that all strains belonged to the genera Micromonospora. The active strains were also identified based on 16S rRNA partial sequence. BLAST search of the 16S rRNA sequences of all tested strains with the sequences available in the NCBI data bank showed a maximum similarity 99% with Micromonospora chersina.

Screening of Rhizobacteria for Plant Growth Promotion and Their Tolerance to Drought Stress RAHAYU FITRIANI WANGSA PUTRIE; ARIS TRI WAHYUDI; ABDJAD ASIH NAWANGSIH; EDI HUSEN
Microbiology Indonesia Vol. 7 No. 3 (2013): September 2013
Publisher : Indonesian Society for microbiology

Rhizobacteria have been known for their capability as plant growth promoter through some mechanisms, directly and indirectly. The purpose of this research to screen rhizobacteria of Bacillus spp. and Pseudomonas spp. for their drought tolerance as plant growth promoter of maize (Zea mays). Screening of rhizobacteria as growth promoter of 47 isolates of Bacillus CR and 34 isolates of Pseudomonas CRB resulted 24 and 9 isolates were able to stimulate the growth of maize sprouts, respectively. Further screening of those growth promoter of the rhizobacterial isolates to drought tolerance resulted 7 isolates of Bacillus CR and 6 isolates of Pseudomonas CRB that were able to grow on medium with osmotic pressure -1 and -2 MPa, respectively. Potential rhizobacterial isolates of growth promoter and drought tolerance were tested for antagonist mechanisms which aims to determine ability to live together in one carrier medium if to be made formulation. Both non antagonist rhizobacterial isolates were evaluated for their potential in producing exopolysaccharide (EPS) revealing that CRB 19 and CR 90 exhibited the highest activity of EPS production up to 0.346 mg mL-1 on medium with -2.0 MPa and 0.107 mg mL-1 on medium with -0.73 MPa, respectively. Based on 16S rRNA sequence analysis, it revealed CRB 19 and CR 90 had highest similarities to Pseudomonas aeruginosa strain B2 and Brevibacillus brevis B33, respectively. Those growth promoter and drought tolerant of Bacillus CR and Pseudomonas CRB had potency to be developed as inoculants in dry land agriculture.

Community Structure of Sporulating Fungi on Decaying Litters of Shorea spp. ISRAWATI HARAHAP; GAYUH RAHAYU; IMAN HIDAYAT
Microbiology Indonesia Vol. 7 No. 3 (2013): September 2013
Publisher : Indonesian Society for microbiology

The community structure of sporulating fungi on decaying branch and leaf litters of Shorea spp. were studied to reveal the common saprobic fungi. The study was mainly based on morphological observation. Twenty-nine species of the sporulating fungi were found on Shorea spp. litters at Situ Gede and Bubulak forest area, Bogor, West Java. The fungi included seven species of Ascomycetes (Annulohypoxylon purpureonitens, Diatrype chlorosarca, Didymosphaeria epidermidis, Lophiostoma sp., Lophodermium sp., Pemphidium sp., and Valsa sp.) and 22 species of anamorphic taxa that consisted of 12 Coelomycetes (Coniella musaiaensis, Coryneum betulinum, Hendersoniopsis thelebola, Lasiodiplodia theobromae, Lasmeniella guaranitica, Leptodothiorella sp., Massariothea themedae, Pestalotia guepinii, Pestalotiopsis sp., Pseudolachnea hispidula, Septoriella sp., and unidentified species of Coelomycetes) and 10 Hyphomycetes (Beltraniella portoricensis, Cryptophialoidea fasciculata, Hermatomyces spaerichus, Kiliophora ubiensis, Minimidochium setosum, Monodisma fragilis, Nodulisporium sp., Stilbella fimetaria, Virgatospora echinofibrosa, and unidentified Hyphomycetes). The most common taxa occuring on decaying leaf litter were B. portoricensis and Pemphidium sp., while those on decaying branch material were L. theobromae and C. fasciculata. The fungal community was subtrate specific. The community on decaying branch litter was more diverse than that on leaf litter. The C/N ratio of the substrate was closely related to the structure of the community.

Production of Acetone, Butanol and Ethanol as Bioenergy Source Materials by Clostridium saccharoperbutylacetonicum N1-4 (ATCC 13564) using Different Substrates HANIES AMBARSARI; KENJI SONOMOTO
Microbiology Indonesia Vol. 7 No. 3 (2013): September 2013
Publisher : Indonesian Society for microbiology

In the effort of developing bioenergy source materials, a laboratorium study was performed to investigate the effects of substrate types and concentrations on the production of acetone-butanol-ethanol (ABE) from various substrates by Clostridium saccharoperbutylacetonicum N1-4 (ATCC 13564) in defined TYA (Tryptone-Yeast extract-Acetate) media. The results demonstrated that the ABE ratios produced by the strain N1-4 were greatly influenced by the types of substrate and the concentrations being used. It was also shown that fermentation of disaccharides (cellobiose and dextrin) by the strain N1-4 could give relatively as high butanol and ABE yields as glucose fermentation after 24 h fermentation time. The strain N1-4 was also found to be able to ferment xylans from birchwood sources producing a relatively high ABE concentration, especially at a prolonged incubation time (after 48 h incubation period). A subsequent experiment using several different concentrations of glucose or cellobiose revealed that the higher the concentration of the substrate being used, then the higher the total ABE concentration or productivity could be obtained but not necessarily in the same increasing ratio. To the best of our knowledge, there was no other previous study about the effect of substrate type and concentration on the ABE ratio by C. saccharoperbutylacetonicum N1-4 (ATCC 13564) using various substrates in defined TYA media, specifically by using xylans and dextrin substrates.

Page 36 of 40 | Total Record : 398

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Home Page

OAI Link

Editorial Team

Contact

Reviewer

Google Scholar

Contact Name Iman Rusmana

Contact Email rusmana13@yahoo.com

Phone +62217560536

Journal Mail Official microbiology.indonesia@gmail.com

Editorial Address kPERHIMPUNAN MIKROBIOLOGI INDONESIA (SeKretariat PERMI), Gedung 10.2 Indonesian Life Sciences Center (ILSC), Zona Bisnis Teknologi Puspiptek, Jalan Raya Serpong - Bogor Gunung Sindur, Jawa Barat 16340, Indonesia. Email: microbiology.indonesia@gmail.com

Location Kota tangerang, Banten INDONESIA

Abstract

Abstract

Abstract

Abstract

Abstract

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Abstract

Abstract

Abstract

Abstract

Contact Name
Iman Rusmana

Contact Email
rusmana13@yahoo.com

Phone
+62217560536

Journal Mail Official
microbiology.indonesia@gmail.com

Editorial Address
kPERHIMPUNAN MIKROBIOLOGI INDONESIA (SeKretariat PERMI), Gedung 10.2 Indonesian Life Sciences Center (ILSC), Zona Bisnis Teknologi Puspiptek, Jalan Raya Serpong - Bogor Gunung Sindur, Jawa Barat 16340, Indonesia. Email: microbiology.indonesia@gmail.com

Location
Kota tangerang,

Banten

INDONESIA