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Microbiology Indonesia

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Published by Perhimpunan Mikrobiologi Indonesia

ISSN : 19783477 EISSN : 20878575 DOI : -

Core Subject : Health, Science,

Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology

Microbiology Indonesia provides a unique venue for publishing original researches in microbiology (espesially from Indonesian reseachers), and ensures that authors could reach the widest possible audience. Microbiology Indonesia publishes a wide range of research disciplines on bacteria, archaea, fungi, protozoa, and virus as well as biotechnology related to microbiology. Topics include (but are not limited to): -methods in microbiology, -bioprocess, -environmental microbiology, -food microbiology, -plant-microbe interaction, -animal-microbe interactions, -microbial community, -microbial genetics, -virology, -comparative and functional microbial genomics, -and gene expression in microbes.

Arjuna Subject : Imunologi dan Mikrobiologi (semua kategori) - Mikrobiologi dan Bioteknologi Terapan

Articles 398 Documents

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Growth Characteristic of Fungus strain MGS-2 in a Defined Medium Containing Organic Acids Derived from Peat Soil DIANA NURANI; KOESNANDAR KOESNANDAR
Microbiology Indonesia Vol. 7 No. 4 (2013): November 2013
Publisher : Indonesian Society for microbiology

Some organic acids derived from lignin degradation in peat soil have significant role in lowering pH of peat soil and have phytotoxic effect for plant. A series of experiments has been conducted to decrease the amount of organic acids in peat soil by microbial treatment. The aim of the study was to characterize the growth of selected fungi using organic acids extracted from peat soil as a sole carbon source.The fungus strain MGS-2 was grown in a liquid medium containing various concentrations of organic acids extracted from peat soil, nitrogen and salt at initial pH of 3.8. The cell growth, pH of medium and the content of organic acids were analyzed. The identification of fungus MGS-2 was based on the 28S rDNA.The ability of Hypocrea sp. to degrade toxic organic acid derived from peat soil has never been reported. The organic acid and nitrogen optimum for the growth were 33.1 mN-NaOH and 2 g NH2SO4 per liter medium, respectively. The interaction between carbon and nitrogen source was found to be significantly influenced by the increment of pH medium, however this interaction did not effect to the cell growth and reduction of organic acids. The carbon source affected significantly to the cell growth and acid metabolism by fungus strain MGS-2 The fungus could not grow well in the medium without yeast exract, but grew well in the limitation of NH2SO4 , suggested that yeast extract was metabolized as nitrogen source. The optimum degradation of organic acids extracted from peat soil by MGS-2 was obtained at pH 3.8. Theseresult suggested that the cell mass production of MGS-2 can be performed in optimal defined medium utilizing organic acid derived from peat soil. Molecular identification revealed that the MGS-2 was closed to Hypocrea sp.

Diversity of Lactic Acid Bacteria Isolated from Indonesian Traditional Fermented Foods APON ZAENAL MUSTOPA; FATIMAH FATIMAH
Microbiology Indonesia Vol. 8 No. 2 (2014): June 2014
Publisher : Indonesian Society for microbiology

The diversity of lactic acid bacteria was evaluated from Indonesian fermented foods such as dadih (buffalo fermented milk), tempoyak (fermented durian), bekasam (fermented meat), and tape ketan (fermented glutinous rice). Lactic acid bacteria were enumerated using selective media and characterised based on a genotypic methods such as rep- PCR and RAPD-PCR, as well as 16S rRNA gene sequencing of representative strains. Forty-six colonies had successfullybeen isolated from Indonesian fermented foods. The great majority of these colonies originated from dadih (43.48%), tempoyak (39.13%), bekasam (13.04%) and tape (4,3%). The 46 isolates were characterised based on a genotypic methods such as RAPD and rep-PCR as well as 16S rRNA gene sequencing of representative strains. The rep-PCR result yielded seven clusters (I-VII) at a similarity level of 75-88% and RAPD-PCR used LB2 primer, M13 primer and primer A, B, C. The RAPD result using LB2 primer yielded eight clusters (I-VIII) at a similarity level of 82-91%. Identification using 16S rRNA showed that the majority strains as Lactobacillus plantarum, Lactobacillus fermentum and Pediococcus pentosaceus strains.

Cloning and Gene Expression of AnsZ Encoding L-Asparaginase Enzyme from Local Bacillus sp. RIMA AZARA; IS HELIANTI; JONI KUSNADI; YUNIANTA YUNIANTA
Microbiology Indonesia Vol. 8 No. 2 (2014): June 2014
Publisher : Indonesian Society for microbiology

L-asparaginase is an enzyme that catalyzes the hydrolysis of L-asparagine into L-aspartic acid and ammonia. In medical aspect, L-asparaginase especially those came from E. coli and Erwinia chrysanthemi used as chemotherapy agent of acute lymphoblastic leukemia (ALL). However, new potential organisms possessing L-asparaginase production capacity with a similar therapeutic effect are still required . In Bacillus subtilis strain 168, there are two kinds of L-asparaginase gene, AnsA and AnsZ. The study of the later L-asparaginase (AnsZ) has not been conducted intensively. The aim of this study is, first, to isolate this gene of L-asparaginase (AnsZ) from local Bacillus sp. and then to express this gene in Escherichia coli. Using PCR-cloning method, an open reading frame (ORF) containing 1128 bp was obtained. The ORF has 99% homology with sequence of L-asparaginase from Bacillus subtilis Bsn5. The gene then was subcloned into pET 21d (+) with his6-tag in the C-terminal of the gene product and expressed in E.coli BL21. L-asparaginase activity analyses showed that recombinant E. coli containing recombinant plasmid with open reading frame (ORF) L-asparaginase (AnsZ) from Bacillus subtilis had higher activity than that is not containing ORF L-asparaginase (AnsZ). Purification with HisPur TM Ni-NTA Purification Kit increased the specific activity of L-asparaginase (AnsZ) enzyme to 29 fold.

Exploration, Isolation and Quantification of β-carotene from Bacterial Symbion of Acropora sp. NAELY K. WUSQY; LEENAWATY LIMANTARA; FERRY F KARWUR
Microbiology Indonesia Vol. 8 No. 2 (2014): June 2014
Publisher : Indonesian Society for microbiology

In the microbial world, pigments are one of the most conspicuous traits. Marine bacteria associated with Acropora sp. collected from Taka Cemara, Karimunjawa Islands were screened for the production of a yellow pigment. The isolation of bacterial symbionts from Acropora sp. on Zobell 2216E medium resulted in one bacterium, KJ5, positively synthesized carotenoids. By reverse phase HPLC analysis, one peak of the pigment types was identified as a β-carotene peak which appeared at 60.24 min. Then, sample of the β-carotene was collected and identified according to their spectral characteristics and compared with the published data in different types of solvent. Based on the HPLC analysis, the total β-carotene contents were calculated by converting the broad absorption of β-carotene. Molecular identification of the bacterium KJ5 using 16S rDNA showed that bacterium KJ5 was closely related to Erythrobacter flavus with 96% homology value.

Population and Diversity of Endophytic Bacteria Associated with Medicinal Plant Curcuma zedoaria TRI RATNA SULISTIYANI; PUSPITA LISDIYANTI; YULIN LESTARI
Microbiology Indonesia Vol. 8 No. 2 (2014): June 2014
Publisher : Indonesian Society for microbiology

Traditionally Curcuma zedoaria (white turmeric) known as herbal medicine which possessing many biological activities. Many endophytic bacteria live in association with their host and may play an important biological roles. The main interest of this study was to investigate the endophytic bacterial diversity associated with white turmeric. White turmerics were collected from three locations in Bogor, West Java, Indonesia. The isolation of endophytic bacteria was carried out using 4 kinds media (Nutrient Agar (NA), NA contained white turmeric extract (NAT), Water Yeast Extract Agar (WYEA), WYEA contained white turmeric extract (WYEAT)), and 2 methods of spread plate and plant piece methods. The identification of selected isolates was conducted by molecular analysis based on 16S DNA. The suitable media and method of isolation endophytic bacteria were NA and spread plate method. A total of 207 bacterial colonies were isolated from rhizomes, stems, and leaves and 73 endophytic bacteria were selected based on morphological characteristics. From them, 32% isolates from Bojong Gede, 22% isolates from Cibinong and 46% isolates from Dramaga were obtained. Endophytic bacteria were predominated 38% in the rhizomes, 32% of stems, and 30% of leaves. Based on 16S rDNA sequence analysis, the isolates were belonging to the cluster Alphaproteobacteria, Betaproteobacteria, Gammaproteobacteria, Firmicutes, and Actinobacteria, with twenty three different genera includes Stenothropomonas, Pseudomonas, Enterobacter, Providencia, Klebsiella, Dickeya, Pantoea, Bacillus, Acinetobacter, Citrobacter, Mycobacterium, Cellulomonas, Microbacterium, Methylobacterium, Penylobacterium, Roseomonas, Agrobacterium, Bosea, Xanthobacter, Rhizobium, Burkholderia, Ralstonia, and Alcaligenes. The plant location, age, part of plant, media and method of isolation seem to influence the endophytic bacterial communities.

Effect of Micro-encapsulated Synbiotic at Different Frequencies for Luminous Vibriosis Control in White Shrimp (Litopenaeus vannamei) WAODE MUNAENI; MUNTI YUHANA; WIDANARNI WIDANARNI
Microbiology Indonesia Vol. 8 No. 2 (2014): June 2014
Publisher : Indonesian Society for microbiology

The aim of this study was to evaluate the effect of micro-encapsulated synbiotic application at different frequencies for luminous disease control in white shrimp (Litopenaeus vannamei). The luminous disease is caused by Vibrio harveyi. In this experiment, a synbiotic which was a combination of the probiotic Bacillus sp. NP5 RfR and the oligosaccharides from sweet potato (Ipomea batatas L.) jago variety was apllied. The synbiotic was encapsulated by spray drying method. The in vivo experiment was conducted by supplementing the shrimp’s diet with the micro-encapsulated synbiotic for 40 days. Treatments included the administration micro-encapsulated synbiotic in different frequencies i.e. once a week (A), twice a week (B), daily (C), and without micro-encapsulated synbiotic (control treatment). The control treatment consisted of positive (K+) and negative (K-) controls. After 30 days period, all of the shrimp were challenged by intramuscular injection of pathogenic V. harveyi RfR at a concentration of 106 CFU ml-1 except the negative control. The treatment C resulted in significantly higher survival rate (SR), specific growth rate (SGR), and immune responses than those of the controls, whereas the feed conversion ratio (FCR) was lower than the controls. In addition, the population of Bacillus sp. NP5 RfR and total bacterial count (TBC) in the intestines increased, whereas the population of V. harveyi RfR and the total vibrio count (TVC) were lower compared to the controls.

Role of Chloramphenicol Acetyltransferase (CAT) Enzyme for Early Detection of Chloramphenicol Resistant Salmonella typhi
Microbiology Indonesia Vol. 8 No. 2 (2014): June 2014
Publisher : Indonesian Society for microbiology

Microbial resistance to antibiotic is a major problem in the treatment of infectious diseases. The purpose ofthis study was to identification the role of CAT enzyme for early detection of chloramphenicol resistant. Observation was performed on isolated from Dr. Sutomo Hospital, Surabaya Health Laboratory, and Microbiology Laboratory-Airlangga University, Surabaya in 2009 and had been subcultured.The sub-culture was then tested for its susceptibility to chloramphenicol by using anti-chloramphenicol acetyl transferase (CAT) antibody from Sigma (catalog number C.9336). Susceptibility test using dilution and diffusion methods proved that resistant could change to sensitive and vice versa. It seems that the CAT enzyme can bind anti-CAT so reversing the resistant into sensitive and conversely, sensitive into resistant one.

Distribution of Rhizopheric actinomycetes on Karst Ecosystem of Gorontalo, Indonesia Ledy Mutmainah Y Syahril; Wirnangsi D Uno; Abubakar Sidik Katili; Yuliana Retnowati
Microbiology Indonesia Vol. 17 No. 1 (2023): March
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5454/mi.17.1.24-30

Karst ecosystem is one type of extreme marginal soil with low chemical, physical and biological fertility. Karst soil is characterized by high calcium (Ca) content, which can affect the availability of essential elements for plants. Actinomycetes are the pioneers of phosphor-poor soil and are distributed on various types of soil, including Karst soil. Distribution, abundance, and diversity of actinomycetes on plant rhizosphere are strongly influenced by soil physicochemical characteristics. The objective of this study was to obtain the actinomycetes and to determine the abundance and distribution in the rhizosphere of various types of plants in the karst ecosystems of Gorontalo. The soil sampling was conducted on the plant's rhizosphere on Bilato, Karang Putih, and Biluhu Karst Hill of Gorontalo based on the purposive sampling method. The physicochemical analysis included humidity, temperature, and soil acidity. The results showed that actinomycetes were found on various plant rhizospheres with an abundance of about 3x102 to 1x104 CFUmL-1. There were five actinomycetes isolated successfully on the plant rhizosphere.The AB-01 isolate was specifically found on Ficus sp. and Scizium sp, the ABT-01 isolates were just distributed on Plumeria abusta, Leucaena leocepala, and Monrngaceae, whereas AKP-01, AKP-02, and AB- 02 were distributed on almost of plant rhizosphere.

Antibacterial Activity of Ethanol Extract of Portulaca oleracea L. Herb from Various Extraction Methods Against Salmonella typhimurium MAULITA CUT NURIA; AULIA EVERESTINA PUJAKA; ERIKA INDAH SAFITRI
Microbiology Indonesia Vol. 15 No. 4 (2021): December
Publisher : Indonesian Society for microbiology

Salmonella typhimurium bacteria could cause gastroenteritis and its growth could be controlled by the active compounds from natural products, which is Portulaca oleracea L. herb. Portulaca oleracea contained tannin, saponins and flavonoids compounds which had different characteristics towards temperature extraction. This study aims to determine the antibacterial activity of ethanol extract of the Portulaca oleracea herb from various extraction methods against S. typhimurium bacteria. Extraction of Portulaca oleracea herb was carried out with four variations methods which were the cold method (maceration and percolation) and heat method (soxhlet and refluxs) using 96% ethanol solvent. The four types of extract were tested for their antibacterial activity by disk diffusion at concentrations of 30%, 35%, 40%, 45% and 50% (b/v). The positive control was chloramphenicol 30 Âµg/disk, while the negative control was DMSO solvent. The results of antibacterial activity test in the form of zone of inhibition were statistically analyzed by Two Way Anova. The results showed that the ethanol extract of the Portulaca oleracea herb from various extraction methods had antibacterial activity against S. typhimurium. There was a significantly difference in the antibacterial activity of ethanol extract of the Portulaca oleracea herb obtained from the reflux method with other methods (maceration, percolation and soxhlet) against S. typhimurium. Keywords: ethanol extract of Portulaca oleracea L. herb, antibacterial, Salmonella typhimurium, various extraction methods

Optimization of Rhizopus spp. Production as Mycoprotein using Soymilk Media Dani Muliawan Halim; Anastasia Tatik Hartanti; Stephanie
Microbiology Indonesia Vol. 16 No. 1 (2022): March
Publisher : Indonesian Society for microbiology

Mycoprotein is food with high protein content, fiber, and low in cholesterol made from fungal mycelium. In this research, mycoprotein was produced by spp. isolated fr Rhizopus om tempeh with soymilk as growth media. This research aims to determine the best strain of spp. and op Rhizopus timum carbon to nitrogen ratio for mycoprotein production Two parameters were applied which w . ere inoculum selection and carbon to nitrogen ratio treatment in media. The best inoculum was selected from four strains of Rhizopus spp., ATH 1,ATH 24,ATHÂ 40, and ATH 53. On the other hand, carbon to nitrogen ratio treatment used were as follows 20:1, 20:2, and 40:2. Mycelium dry weight and protein content were measured, as well as reduction sugar, dissolved protein and total volatile base nitrogen concentration in media. The best strain for producing biomass was ATH 24 with 0.6 g of 0 mycelium dry weight per 50 mL of media and the protein content was 0.236 g. The best carbon to nitrogen ratio treatment was 20:1 with 0.57 gram of mycelium dry weight per 50 mLof media and the protein content was 0.20 g. Thus, our data indicate that strain ATH 24 with 20:1 of carbon to nitrogen ratio in media were highly potential for producing mycoprotein. Keywords: fermentation, mycelium, mycoprotein, Rhizopus, soymilk

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Home Page

OAI Link

Editorial Team

Contact

Reviewer

Google Scholar

Contact Name Iman Rusmana

Contact Email rusmana13@yahoo.com

Phone +62217560536

Journal Mail Official microbiology.indonesia@gmail.com

Editorial Address kPERHIMPUNAN MIKROBIOLOGI INDONESIA (SeKretariat PERMI), Gedung 10.2 Indonesian Life Sciences Center (ILSC), Zona Bisnis Teknologi Puspiptek, Jalan Raya Serpong - Bogor Gunung Sindur, Jawa Barat 16340, Indonesia. Email: microbiology.indonesia@gmail.com

Location Kota tangerang, Banten INDONESIA

Abstract

Abstract

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Abstract

Abstract

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Abstract

Contact Name
Iman Rusmana

Contact Email
rusmana13@yahoo.com

Phone
+62217560536

Journal Mail Official
microbiology.indonesia@gmail.com

Editorial Address
kPERHIMPUNAN MIKROBIOLOGI INDONESIA (SeKretariat PERMI), Gedung 10.2 Indonesian Life Sciences Center (ILSC), Zona Bisnis Teknologi Puspiptek, Jalan Raya Serpong - Bogor Gunung Sindur, Jawa Barat 16340, Indonesia. Email: microbiology.indonesia@gmail.com

Location
Kota tangerang,

Banten

INDONESIA