Garuda - Garba Rujukan Digital

p-Index From 2020 - 2025

2.56

P-Index

This Author published in this journals

All Journal HAYATI Journal of Biosciences Buletin Agrohorti Jurnal Ilmu Pertanian Indonesia Jurnal Fitopatologi Indonesia Microbiology Indonesia BIOTROPIA - The Southeast Asian Journal of Tropical Biology Biogenesis: Jurnal Ilmiah Biologi Jurnal Hortikultura AGRIVITA, Journal of Agricultural Science Indonesian Journal of Biotechnology JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Jurnal Entomologi Indonesia Makara Journal of Science

Abdjad Asih Nawangsih

Department Of Plant Protections, Faculty Of Agriculture, Institut Pertanian Bogor, Jalan Agatis Kampus Dramaga Bogor 16680, Indonesia

Author-ID : 217688

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Veterinary

Published : 32 Documents Claim Missing Document

Claim Missing Document

Articles

1 2 3 4

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.18.2.66

Biological control of bacterial wilt pathogen (Ralstonia solanacearum) of tomato using endophytic bacteria is one of the alternative control methods to support sustainable agriculture. This study was conducted to select and characterize endophytic bacteria isolated from healthy tomato stems and to test their ability to promote plant growth and suppress bacterial wilt disease. Among 49 isolates successfully isolated, 41 were non-plant pathogenic. Green house test on six selected isolates based on antagonistic effect on R. solanacearum or ability to suppress R. solanacearum population in dual culture assays obtained BC4 and BL10 isolates as promising biocontrol agents. At six weeks after transplanting, plants treated with BC4 isolate showed significantly lower disease incidence (33%) than that of control (83%). Plants height was not significantly affected by endophytic bacterial treatments. Based on 16S rRNA sequence, BC4 isolate had 97% similarity with Staphylococcus epidermidis (accession number EU834240.1), while isolate BL10 had 98% similarity with Bacillus amyloliquefaciens strain JK-SD002 (accession number AB547229.1).

Pemanfaatan Bakteri Pseudomonas Flourescens Rh4003 dan Asam Askorbat untuk Mempertahankan Viabilitas Benih Padi Hibrida A. A. Keswari Krisnandika; Eny Widajati; Abdjad Asih Nawangsih
Buletin Agrohorti Vol. 5 No. 2 (2017): Buletin Agrohorti
Publisher : Departemen Agronomi dan Hortikultura

Padi hibrida memiliki glume terbuka yang berperan penting pada laju perlambatan viabilitas benih selama penyimpanan. Serta untuk mencapai hasil panen padi hibrida yang optimal membutuhkan asupan nutrisi yang berkualitas tinggi. Penelitian ini bertujuan untuk mempelajari pengaruh formula lapisan benih dengan penambahan bakteri Pseudomonas fluorescens RH4003 sebagai Pertumbuhan Tanaman Memnghasilkan Rhizobakteri dan asam askorbat sebagai antioksidan, menghambat viabilitas benih padi hibrida dalam penyimpanan. Penelitian ini terdiri dari tiga seri percobaan, yaitu menggunakan 3 varietas padi hibrida (DG-1, Intani dan SL-8), Rancangan percobaan yang digunakan yaitu petak bersarang dengan enam kali periode penyimpanan sebagai petak utama. Hasil penelitian menunjukkan bahwa lapisan yang menggunakan asam askorbat merupakan perlakuan terbaik dari semua variabel viabilitas hampir pada semua varietas. Perlakuan dengan menggunakan bakteri memiliki hasil yang signifikan (p < 0.01) meningkatkan laju pertumbuhan dan indeks vigor pada minggu keenam penyimpanan sebesar 22.48% per etmal dan 83.33% untuk varietas benih padi SL-8. Lapisan DG-1 yang menggunakan asam askorbat pada minggu keenam mengakibatkan indeks vigor tertinggi (90 %) yang signifikan (p<0.5) dan peningkatan yang sangat signifikan pada perkecambahan benih dan indeks vigor minggu kesembilan penyimpanan untuk varietas SL-8 SHS (92.67% dan 73.33%).

Pemanfaatan Bakteri Endofit pada Pisang untuk Mengendalikan Penyakit Darah: Isolasi, Uji Penghambatan In Vitro dan In Planta Abdjad Asih Nawangsih
Jurnal Ilmu Pertanian Indonesia Vol. 12 No. 1 (2007): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (194.93 KB)

Blood desease caused by blood disease bacterium is one of the important disease of banana in Indonesia. Disease control using resistant varieties was difficult to be practiced because the pathogen can attack all varieties of banana. Sanitation and eradication has been done by farmers but the desease still occured in the field with high incidence. Aplication of same rhizosphere bacteria failed to control the desease in the field.Keyword: Bacillus spp., Pseudomonas spp., endophytic bacteria, blood disease bacterium, banana

Pemanfaatan Mulsa Jerami dan Plant Growth Promoting Rhizobacteria untuk Menekan Penyakit Pustul Bakteri pada Tanaman Kedelai Tita Widjayanti; Abdjad Asih Nawangsih; Kikin Hamzah Mutaqin
Jurnal Fitopatologi Indonesia Vol 8 No 6 (2012)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Bacterial pustule caused by Xanthomonas axonopodis pv. glycines is one of important bacterial diseases of soybean in Indonesia. The development of alternative control of the disease is required to support integrated diseases management for sustainable agriculture. This research was conducted to observe the effect of straw mulch and plant growth promoting Rhizobacteria (PGPR) on the incidence of bacterial pustule disease on two soybean varieties in the farmer field at Cigombong, Sukabumi, West Java. The result of this research showed that all of the factors as combinations did not significantly affect the value of total area under disease progress curve (AUDPC) of bacterial pustule. Factor that significantly affected the values of AUDPC was soybean varieties. Gepak Kuning variety has lowest AUDPC value compared to Anjasmoro variety. In addition, combination of Gepak Kuning variety and straw mulch showed significantly different effect on AUDPC value. Therefore, this treatment combination should be recommended for the farmers.Key word: PGPR, soybean variety, straw mulch, Xanthomonas axonopodis pv. glycines

Keefektifan Bakteri Endofit dan Bakteri Perakaran Pemacu Pertumbuhan Tanaman dalam Menekan Penyakit Layu Bakteri pada Tomat Zhenita Vinda Tri Handini; Abdjad Asih Nawangsih
Jurnal Fitopatologi Indonesia Vol 10 No 2 (2014)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Bacterial wilt disease caused by Ralstonia solanacearum is an important disease on tomato. Endophytic bacteria and plant growth promoting rhizobacteria has been reported as potential biocontrol agent for many plant diseases. Research was conducted to determine the effectiveness of some isolates of endophytic bacteria (BC10, Bacillus amyloliquefaciens, dan Staphylococcus epidermidis BC4) and plant growth promoting rhizobacteria (Pseudomonas fluorescence RH4003, Bacillus subtilis AB89) as biocontrol agents for tomato wilt disease. Bacterial isolates was applied as soil drench on tomato seedling in single or combination treatments. Combination of P. fluorescens RH4003 and isolate BC10 (F1*BC10) enhanced the highest plant height, although plant dry mass among treatments was not significantly different. Single application of S. epidermidis BC4 was significantly suppressed disease incidence.

Interaksi antara Bakteri Endofit dan Bakteri Perakaran Pemacu Pertumbuhan Tanaman dalam Menekan Penyakit Layu Bakteri pada Tomat Abdjad Asih Nawangsih; Fitri Fatma Wardani
Jurnal Fitopatologi Indonesia Vol 10 No 5 (2014)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

One of the factors affected production of tomato is the incidence of bacterial wilt disease caused by Ralstonia solanacearum. An alternative control to manage the disease which is environmentally friendly is the use of biocontrol agents. Application of endophytic bacteria (Staphylococcus epidermidis BC4) and plant growth promoting rhizobacteria (Pseudomonas fluorescens RH4003 and Bacillus subtilis AB89) as biocontrol of tomato bacterial wilt was expected to be an alternative method. The objective of this study is to evaluate the interaction among endophytic bacteria and PGPR to suppress the development of bacterial wilt disease (R. solanacearum) and promoting the growth of tomato. Bacterial treatment which caused the highest suppression on disease incidence was single application of P. fluorescens RH4003. Disease index caused by S. epidermidis BC4 and P. fluorescens RH4003 applied individually was up to 41.18 dan 45.88%, respectively. Interaction between the endophytic bacteria and both of the PGPRs were antagonistic.

Deteksi dan Identifikasi Dickeya sp. sebagai Organisme Pengganggu Tumbuhan Karantina A2 pada Tanaman Kentang di Jawa Haerani Haerani; Abdjad Asih Nawangsih; Tri Asmira Damayanti
Jurnal Fitopatologi Indonesia Vol 11 No 4 (2015)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Erwinia chrysanthemi (currently Dickeya sp.) is one of the A2 quarantine pest that must be concerned of its distribution on potato in Indonesia. The aim of this study is to detect and identify E. chrysanthemi from potato in Java. A total of 400 samples of potato plants showing symptoms of soft rot were obtained from several potato areas in Pangalengan and Garut (West Java), Dieng (Central Java), and Batu-Malang (East Java). Disease incidence was determined by indirect enzyme-linked immunosorbent assay (I-ELISA) using polyclonal antiserum. E.chrysanthemi was isolated from plant samples with positive ELISA results. Furthermore, bacterial isolates were characterized by GEN III OmniLog ID System and PCR using specific primers Ec3F/Ec4R, as well as the universal 16S rRNA primer pair of 27F/1429R. The incidence of E. chrysanthemi based on ELISA was obtained. Based on physiological characters; Gram, catalase, oxidase, and oxidation-fermentation, there were 4 isolates similar to the genus of Erwinia. However, the results of Gen III OmniLog System, PCR, and nucleotide sequences analysis of 16S rRNA confirmed that none of the isolates were identified as E.chrysanthemi. Otherwise, those 4 isolates were identified as Pseudomonas oryzihabitans, Pantoea agglomerans, and Pseudomonas viridiflava. The result of this study indicated that the existence of E. chrysanthemi as an A2 quarantine pest on potato in Java can not be confirmed and remains as an A1 quarantine pest.

Bakteri Endofit asal Tanaman Tembakau sebagai Agens Pengendali Meloidogyne spp. isnainy dinul Mursyalatiyus; Abdul Munif; Abdjad Asih Nawangsih
Jurnal Fitopatologi Indonesia Vol 14 No 6 (2018)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Endophytic Bacteria from Tobacco Plant as Biocontrol Agent of Meloidogyne spp.Soilborne disease on tobacco plants caused by fungal and bacterial infection in association with root-knot nematode (Meloidogyne spp.) may cause significant yield loss. Endophytic bacteria have been recognized as biological control agent for Meloidogyne spp. as well as plant promoting growth agent. Research was conducted to evaluate endophytic bacteria isolated from tobacco plants as biological control agent for Meloidogyne spp. infecting tobacco. A total of 215 isolates of endophytic bacteria were isolated from root of two tobacco varieties, Kemloko and Prancak 95. Biosafety screening showed that 80 isolates (37%) and 7 isolates (8%) gave negative reaction on hypersensitivity test and hemolysis test, respectively. Seven isolates i.e. TPT3.10, TPT2.1, TK3n8, TK2t21, TK2n8, TK3n1 and TK2t11 were able to promote plant growth and increase the mortality of juvenile Meloidogyne spp. Physiological characterization of endophytic bacteria showed that most of the isolates were able to produce protease enzyme, phosphate, nitrogen and HCN. The same isolates were also able to suppress the number of galls from 80.09% up to 93.82%. Two isolates, TPT3.10 and TK2n8, are considered having the best suppression on root gall formation.

Uji In Vitro Asap Cair terhadap Ralstonia syzygii subsp. celebesensis Penyebab Penyakit Darah pada Pisang Aisyah Imas; Giyanto Giyanto; Meity Suradji Sinaga; Abdjad Asih Nawangsih; Gustan Pari
Jurnal Fitopatologi Indonesia Vol 14 No 4 (2018)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

In Vitro Test of Liquid Smoke against Ralstonia syzygii subsp. Celebesensis, the Cause of Blood Disease in Bananas Blood disease, caused by Ralstonia syzygii subsp. celebesensis, is an important disease of banana plants in Indonesia. Several control methods have been done, but the result were not effective. Liquid smoke from wood waste is reported to be antimicrobial, but its use for controlling R. syzygii subsp. celebesensis has never been reported. Research was conducted to examine the ability of liquid smoke produced from coconut shell (CS-LS), pinecone (P-LS), and oil palm branch (OPB-LS) in inhibiting the growth of R. syzygii subsp. celebensensis in vitro and its effect on bacterial cell morphology. Efficacy test of liquid smoke was carried out by agar diffusion method and measurement of bacterial density by spectrophotometry with ʎ 600 nm. Observation of bacterial cell morphology was carried out by electron microscopy. The CS-LS, P-LS, and OPB-LS starting from 0.5% concentration showed inhibitory activity against R. syzygii subsp. celebesensis both on triphenyl tetrazolium chloride medium and luria bertani broth. Liquid smoke also caused damage to cell walls and cell membranes. Therefore, liquid smoke has the potential to be used as component in control method for R. syzygii subsp. celebesensis.

Asap Cair Sebagai Pemacu Pertumbuhan dan Ketahanan Tanaman Pisang terhadap Ralstonia syzygii subsp. celebesensis Muhammad Evan Nurrahmawan; Giyanto; Abdjad Asih Nawangsih; Erina Sulistiani
Jurnal Fitopatologi Indonesia Vol 17 No 5 (2021)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14692/jfi.17.5.183-194

Bibit pisang hasil kultur jaringan diketahui rentan terhadap cekaman pada awal pertumbuhan di lapangan. Pra-pengondisian bibit menggunakan agens priming dilaporkan meningkatkan pertumbuhan dan ketahanan tanaman. Asap cair tempurung kelapa dilaporkan mampu memacu pertumbuhan dan menginduksi ketahanan tanaman. Penelitian ini bertujuan mendapatkan informasi pengaruh asap cair asal tempurung kelapa terhadap pertumbuhan dan aktivitas enzim ketahanan pada plantlet pisang Cavendish pada fase induksi perakaran serta penekanan R. syzygii subsp. celebesensis secara in vitro. Tahapan penelitian mencakup uji fitotoksisitas asap cair, analisis pertumbuhan plantlet, analisis aktivitas enzim peroksidase dan polifenol oksidase, isolasi R. syzygii subsp. celebesensis, dan uji toksisitas asap cair terhadap R. syzygii subsp. celebesensis secara in vitro. Hasil penelitian menunjukkan perlakuan asap cair pada konsentrasi ≥ 1.5% bersifat fitotoksik dengan gejala meliputi klorosis, nekrosis, terbentuk lendir dan kematian plantlet. Perlakuan asap cair pada konsentrasi ≤ 1% tidak bersifat fitotoksik, bahkan peningkatan pertumbuhan plantlet optimum ditunjukkan pada perlakuan asap cair 0.1%. Perlakuan asap cair menyebabkan peningkatan aktivitas enzim ketahanan pada 2, 4 dan 6 hari setelah tanam (HST), tetapi menurun pada 30 HST. Selain itu, asap cair bersifat antibakteri melalui terbentuknya zona hambat dan menyebabkan penurunan nilai kerapatan sel R. syzygii subsp. celebesensis. Penelitian ini menunjukkan potensi teknik priming untuk pengendalian penyakit darah pisang terutama pada bibit pisang hasil kultur jaringan.

Co-Authors . Giyanto . Giyanto Abdul Munif ABDUL MUNIF ABDUL MUNIF Abidin, Anugrawati Af'idzatuttama Af’idzatuttama Aisyah Imas Alina Akhdiya Anak Agung Keswari Krisnandika Anja Meryandini Aris Tri Wahyudi Asysyuura, Asysyuura Boedi Tjahjono Budi Marwoto DIMAS ANDRIANTO Edelwinna, Tiara EDI HUSEN Efi Toding Tondok Eka Wijayanti Eka Wijayanti Ely Lailatul Maghfiroh Eny Widajati Erina Sulistiani Firdaus, Muhamad Fajar Fitri Fatma Wardani Giyanto Gustan Pari Gustan Pari Haerani Haerani Hanudin Hanudin Husda Marwan Ida Parida IKA DAMAYANTI Iman Rusmana Imas Aisyah isnainy dinul Mursyalatiyus Juang Gema Kartika Kikin H Mutaqin Krisnandika, A. A. Keswari Marianah, Lisa Meity S. Sinaga Meity Suradji Sinaga Meity Suradji Sinaga Meliah, Siti Muhammad Evan Nurrahmawan Purwanto, Ukhradiya Magharaniq Safira RAHAYU FITRIANI WANGSA PUTRIE Sigiro, Ria Heni Siti Zulaiha Sudir Sudir, Sudir Sulistiyani Suryo Wiyono Tita Widjayanti Tita Widjayanti . Toding Tondok, Efi TRI ASMIRA DAMAYANTI Umi Fatmawati Wati, Cheppy Wiraswati, Sri Martina Yana Anisa . YULIN LESTARI Zhenita Vinda Tri Handini

Title

Found 32 Documents
Search

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Title Search

Found 32 Documents Search

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Title

Found 32 Documents
Search