Garuda - Garba Rujukan Digital

All

BIOTROPIA - The Southeast Asian Journal of Tropical Biology

biotropia
Website

Published by Southeast Asian Regional Centre for Tropical Biology

ISSN : 02156334 EISSN : 1907770X DOI : http://dx.doi.org/10.11598/btb

Core Subject : Health, Science, Agriculture,

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Veterinary

BIOTROPIA, The Southeast Asian Journal of Tropical Biology, is a scientific publication of the Southeast Asian Ministers of Education Organization (SEAMEO) – Southeast Asian Regional Center for Tropical Biology (BIOTROP). It publishes articles in the broad areas of tropical ecosystems and environmental impacts, biodiversity conservation and sustainable development and biotechnology.

Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Pertanian dan Biologi (Lain-Lain)

Articles 520 Documents

7 8 9 10 11

ENDIANDRA KASSAMENSIS (LAURACEAE), A NEW SPECIES FROM NEW GUINEA Arifiani, Deby
BIOTROPIA Vol. 19 No. 2 (2012): BIOTROPIA Vol. 19 No. 2 December 2012
Publisher : SEAMEO BIOTROP

A new species of Endiandra (Lauraceae) is described from New Guinea. Endiandra kassamensis is described based on specimens collected over four decades ago. Unlike most Endiandra which grow in lowland forest, E. kassamensis is found in high altitude forest. The species is characterized by the presence of staminodia with the absence of staminal glands. Â Key words: Endiandra, Lauraceae, staminal glands, staminodia, endemic, New Guinea

Agrobacterium-Mediated Genetic Transformation of Seaweed Kappaphycus alvarezii Using Gα Gene and Callus Cultures Sulistiani, Erina; Suharsono, Suharsono; Supena, Ence Darmo Jaya; Miftahudin, Miftahudin
BIOTROPIA Vol. 26 No. 1 (2019): BIOTROPIA Vol. 26 No. 1 April 2019
Publisher : SEAMEO BIOTROP

Cottonii seaweed (Kappaphycus alvarezii Doty) is one of the most important commercial sources of carrageenan, which is widely used in the pharmaceutical and food industries. A major problem in the cultivation of this seaweed is ice-ice disease, which is caused by extreme changes in environmental conditions such as temperature and seawater salinity. Gene transformation to produce transgenic Kappaphycus lines that are tolerant to environmental stress is a potential solution to this problem. The Gα gene, which encodes the heterotrimeric G-protein alpha subunit, plays an important role in tolerance to biotic and abiotic environmental stress. This study aimed to: (a) introduce the Gα gene into the callus cells of K. alvarezii and regenerate transformed callus cells into transgenic plantlets; and (b) determine the appropriate concentration of acetosyringone and Agrobacterium tumefaciens strain for successful gene transfer into the callus of K. alvarezii. The callus cells of K. alvarezii were transformed using Agrobacterium tumefaciens strains LBA4404 and EHA105 carrying the expression vector pGWB502-Gα under the control of the CaMV-35S promoter. The calli and A. tumefaciens were co-cultivated in different concentrations of acetosyringone (20, 40, and 60 mg/L). The regeneration of transformed callus cells into transgenic plantlets was successfully achieved using the somatic embryogenesis technique. The results showed that the highest percentage of putative transgenic micropropagule formation occurred at acetosyringone concentrations of 20–40 mg/L. Polymerase chain reaction (PCR) analysis of twenty regenerated plantlets indicated that the Gα gene was successfully introduced into the genomic DNA of all samples. The highest transformation efficiency was obtained from the 20–40 mg/L acetosyringone co-cultivation treatment (22–28%). The transformation efficiency produced by Agrobacterium tumefaciens EHA105 (23%) was not significantly different from that produced using strain LBA4404 (15%).

NUTRIENT STOCKS IN FOUR STAGES OF A LOWLAND RAIN FOREST AT PASIRMAYANG, JAMBI, CENTRAL SUMATRA, LESTARI, PAMUJI
BIOTROPIA No. 11 (1998)
Publisher : SEAMEO BIOTROP

Studies of nutrient cycling of tropical forests should differentiate between dynamic stages of the forest. We studied the nutrient concentration (N, P, K, Ca and Mg) and phytomass of aboveground (living and non living parts) and belowground compartments (soil) in four dynamic stages, namely Building (Bl and B2), and Mature (Ml and M2) stages, for a lowland rain forest. Nutrient concentrations in various compartments differed between the dynamic stages. Bark contains higher nutrient concentration than wood parts, both in stems and branches. Leaves contain higher nutrient concentration than wood parts, both in litterfall and litter. The concentration of K in throughfall is the highest, Ca and Mg perform similar value. Throughfall exhibits lower nutrient concentration than open area rain water. The nutrient concentration of 10-20 cm is higher than in the 0-10 cm soil depth. The phytomass values are highly variable among tree parts, diameter classes and dynamic stages. The phytomass is generally the highest in M2 and significantly different from Bl ,Â Ml and B2. The phytomass of leaves in litterfall and litter is higherÂ than wood parts. More litterfall and litter are accumulated in mature than building phases. Key words : nutrient / phytomass / dynamics / rain / forest

Soil Properties and Tree Composition in a 27-Year Old Acacia mangium Willd. Plantation on Abandoned Mining Area at Phangnga Forestry Research Station Wongprom, Jetsada; Poolsiri, Roongreang; Diloksumpun, Sapit; Ngernsaengsaruay, Chatchai
BIOTROPIA Vol. 27 No. 2 (2020): BIOTROPIA Vol. 27 No. 2 August 2020
Publisher : SEAMEO BIOTROP

In order to validate the important role of Acacia mangium plantation in mining rehabilitation, a study was conducted on the soil properties and tree composition in a 27-year-old A. mangium plantation growing on sandy (S27) and clay (C27) soil types, as well as a mixed plantation (MP) growing on clay soil type. The results were compared with those grown on an abandoned mining area (AB), a secondary forest (SF) and a primary forest (PF), at the Phangnga Forestry Research Station, Thailand. Three 40 x 40 m permanent plots were established and soil samples were randomly collected from depths of 0-10, 10-20, 20-30, and 30-50 cm, in each of the S27, C27, MP, AB, SF, and PF. The bulk density values in the S27, C27, and MP were lower than that in AB and was similar to those in SF and PF, particularly, the top soil. Total nitrogen, available phosphorus, organic matter, exchangeable potassium, and magnesium contents in the S27, C27, and MP were higher than that in AB, but were lower than those in SF and PF, indicating that the soil development in the S27, C27, and MP was slower than in the SF and PF. This lower bulk density values and higher soil nutrient contents were positively contributed by the A. mangium plantation. The Shannon-Wiener index obtained for S27 (1.43), C27 (2.51), and MP (2.77) were lower than that for the SF (3.86). The similarity indices of the tree species found in S27, C27, MP, and PF were low, ranging from 5.83-8.00, indicating that the development of the forest community was slow compared to SF (31.03). Enrichment planting with poorly dispersed shade tolerant trees has increased the diversity and improved the forest structure in the mined out areas and other similarly degraded lands.

CHARACTERIZATION OF THREE BENZOATE DEGRADING ANOXYGENIC PHOTOSYNTHETIC BACTERIA ISOLATED FROM THE ENVIRONMENT DWI SURYANTO; ANTONIUS SUWANTO; ANJA MERYANDINI
BIOTROPIA - The Southeast Asian Journal of Tropical Biology No. 17 (2001)
Publisher : SEAMEO BIOTROP

ThreeÂ anoxygenic photosynthetic bacteria, DS-1, DS-4 and Cas-13, have been examinatedÂ forÂ themorphological and physiologicalÂ properties. All strains were rod-shape cells withÂ a swollen terminalÂ endGramÂ negative, motile, non-halophilic, non-alkalophilicÂ and non-acidophilic,Â and capable of utilizinbenzoate aerobically and photo-anaerobically. Sequence analysis of part of 16S rRNA genes showed that DS1 and Cas-13 were closely related to Rhodopseudomonas palustris Strain 7 with a similarity of 97%, whereaDS-4 may not be closely related to the former two strains with a similarity of 78% based on the constructephylogenicÂ tree. Spectral analysis indicated that the threeÂ bacteriaÂ hadÂ bacteriochlorophylÂ aÂ and normaspirilloxanthin series. Growth in medium enriched with vitamin and supplemented with benzoate as their sole C-sources wabetter than in medium without vitamin. Benzoate degradation in medium with vitamin was accelerated. ThabilityÂ to grow on benzoate without added vitamins indicatedÂ thatÂ the bacteria were able to synthesizeÂ theown vitamins. Key words: anoxygenic photosynthetic bacteria/ benzoate degradation/ 16S rRNA gene.

RAPD Analysis to Detect Somaclonal Variation of Pineapple In Vitro Cultures during Micropropagation Roostika, Ika; Khumaida, Nurul; Ardie, Sintho Wahyuning
BIOTROPIA Vol. 22 No. 2 (2015): BIOTROPIA Vol. 22 No. 2 December 2015
Publisher : SEAMEO BIOTROP

Plant off-type formation has become a concern in pineapple micropropagation, and reliable methods are required to detect and minimize this problem. This study was conducted to confirm the occurrence of somaclonal variation during the micropropagation of pineapple clone Simadu. The effects of culture period (long vs. short duration) and regeneration methods (direct organogenesis, indirect organogenesis, and somatic embryogenesis) were evaluated to determine their contribution to somaclonal variation. RAPD analysis using ten primers was performed to confirm genetic variation. The results showed that RAPD assays could be applied for early detection of somaclonal variation in pineapple, with OPA primers performing better than OPJ primers. Phenotypic variation observed in four-year-old field-grown pineapple plants and plantlets was associated with genetic variation. The findings demonstrated that the long culture period was the main factor contributing to somaclonal variation, while regeneration method and plant growth regulators also played a role in inducing genetic changes. Newly initiated cultures showed a higher level of genetic similarity. Therefore, applying an appropriate micropropagation strategy is necessary to minimize plant off-types. It is recommended to avoid using long-term cultures as mother stock and to apply direct organogenesis rather than indirect organogenesis or somatic embryogenesis for pineapple micropropagation.

ETHNOBOTANICAL STUDY OF DIGESTIVE SYSTEMS DISORDERS IN BADUY ETHNIC, INDONESIA Rida Oktorida Khastini; Indria Wahyuni; Irma Saraswati
BIOTROPIA - The Southeast Asian Journal of Tropical Biology Vol. 28 No. 1 (2021): BIOTROPIA Vol. 28 No. 1 April 2021
Publisher : SEAMEO BIOTROP

Digestive disorders rank among the most common problems faced in Indonesia, especially for the Baduy people in Banten Province. Although the Baduy live in water-rich areas, their lack of sanitation facilities and unawareness of methods of disease prevention have prompted high morbidity and mortality rates in their communities, largely due to digestive system disorders that they continue to treat with medicinal plants. This survey was undertaken to document Baduy indigenous medicinal plants that were used to treat and prevent different types of digestive system disorders in their communities using quantitative ethnobotanical approaches. Ethno medicinal data were collected from 30 informants regarding their knowledge on medicinal plants. Quantitative approaches were used to determine the use value and informant consensus factor values of collected data. The results revealed that the Baduy currently use 54 medicinal plant species belonging to 30 families to treat digestive system disorders. Additional research is required, however, to validate the function of the medicinal plants and identify their active compounds.

DEVELOPMENT OF ANTIBODY TO RALSTONIA SOLANACEARUM AND ITS APPLICATION FOR DETECTION OF BACTERIAL WILT SURYADI, YADI
BIOTROPIA Vol. 16 No. 2 (2009): BIOTROPIA Vol. 16 No. 2 December 2009
Publisher : SEAMEO BIOTROP

The serological assay for the detection of bacterial wilt caused by Ralstonia solanacearum RSwas able to provide information regarding the presence of the pathogen in plant materials Theresearch is was aimed to develop polyclonal antibody PAb for RS detection Bacterial wholecells of RS isolates mixed with glutaraldehyde were used to immunize New Zealand femalewhite rabbit The titre of antibody in culture supernatant was 1 1024 The PAb developed froma ground nut RS isolates reacted with infected plant samples from various locations It was ableto detect RS antigen of crude extract and pure cultures from tomato and potato plant samples45 using dot blot ELISA however the minimum detectable concentration of RS antigen was 10cellsml The PAb obtained in this study is sensitive enough to detect RS isolates in routineserological assayKey words Bacterial wilt Ralstonia solanacearum antibody dot blot ELISA

PRESERVATION OF GARUT RAMS SPERMATOZOON AS A SOURCE OF MALE GERM PLASM ARIEF BOEDIONO; HERDIS; MUHAMMAD RIZAL'
BIOTROPIA - The Southeast Asian Journal of Tropical Biology No. 23 (2004)
Publisher : SEAMEO BIOTROP

This study was conducted to examine the quality of ejaculated sperm by Garut rams to be used for artificial insemination (AI) and viability of sperm that were collected from preserved cauda epididymisÂ (4Â°C up to 12 days) for assisted reproductive technology. The semen was collected by artificial vagina, with the sperm motility, live sperm, acrosomal intact, and intact plasma membrane observed. Sperm motility was 75%, while for the live sperm, intact plasma membrane and sperm abnormality were 91.5%, 90.0%, and 1.8%, respectively. In the other study, sperm was collected from cauda epididymis by aspiration method and diluted in different media: 1) Brackett Oliphant (BO) media and 2) modified Phosphate Buffer Saline (mPBS). Evaluation ofÂ sperm motility and intact plasma membrane were conducted after washing, counting and dilution of the sperm. The results of this study showed that the sperm motility and intact plasma membrane could be maintained better in BO rather than PBS medium although they were not statistically different (P>0.05). At day 12 of preservation, the motility and intact plasma membrane of sperm collected from cauda epididymis were 0.7% and 1.33% for motility and plasma membrane intact, respectively. These findings showed that the Garut rams semen was qualified for AI and frozen processing; in vitro embryo production by introducing the assisted reproductive technology such as intracytoplasmic sperm injection (ICSI) could be applied by using the sperm collected from preserved cauda epididymis until 12 days of preservation at 4Â°C. Keywords :Â Â Reproduction/spcrmatogenesis/inscmination/Garut rams/small ruminant

Optimization and Efficiency Improvement of Inhouse Immunoaffinity Column Ku-AF02 for -Aflatoxin Detectionoptimization and Efficiency Improvement of Inhouse Immunoaffinity Column Ku-AF02 for Aflatoxin Detection Kooprasertying, Phakpoom; Maneeboon, Thanapoom; Iamtaweejaroen, Panrapee; Hongprayoon, Ratchanee; Mahakarnchanakul, Warapa
BIOTROPIA Vol. 24 No. 2 (2017): BIOTROPIA Vol. 24 No. 2 August 2017
Publisher : SEAMEO BIOTROP

In-house immunoaffinity column for aflatoxins (AFs) detection is first developed by Kasetsart University since 2011, i.e. the first IAC prototype, KU-AF01. Detection of aflatoxin B (AFB) up to 200 ng is considered significantly efficient, but the production cost is considered high. The high cost was due to the cost of monoclonal antibody specific to aflatoxin and its supporting materials, i.e. CNBr-activated Sepharose 4B. Therefore, this study was aimed to improve the efficiency of in-house immunoaffinity columns by replacing CNBr-activated Sepharose 4B with other supporting materials and to lower the ratio of antibody to supporting materials. Response surface methodology (RSM) was applied to determine the optimum alternative supporting materials and the ratio. Results revealed that when compared with CNBr-activated Sepharose 4B, all materials tested could recover higher than 80% AFB, except the activated CH Sepharose 4B. Results also indicated that the optimum ratio was 0.4 mg anti-aflatoxin monoclonal antibody to 204 μL CNBr-activated Sepharose 4B. CNBr-activated Sepharose 4B was considered to provide the best precision in recovering AFB. KU-AF02 increased the ability to detect AFB to 500 ppb. The recovery of AFs in the reference materials using KU-AF02 was more than 96% successful, with HorRat value range of 0.34–0.76. The reference materials used were 2 levels of AFs in peanuts, i.e. 47.68 and 72.14 ng/g. In terms of quality control in IAC production, there were no significant differences among the 5 batches produced (p > 0.05). KU-AF02 demonstrated stable and constant percent recovery at 4°C for up to 12 months (tested with standard 200 ppb AFB). This study indicated that KU-AF02 could lower the ratio of supporting materials to the antibody and that KU-AF02 has high stability and has good ability for cleaning up AFs. Therefore, KU-AF02 is recommended to be used as an in-house immunoaffinity column for aflatoxin detection in Thailand.

Page 9 of 52 | Total Record : 520

7 8 9 10 11

Filter by Year

1987 2025

Filter By Issues

All Issue Vol. 32 No. 2 (2025): BIOTROPIA Vol. 32 No. 2 August 2025 Vol. 32 No. 1 (2025): BIOTROPIA Vol. 32 No. 1 April 2025 Vol. 31 No. 3 (2024): BIOTROPIA Vol. 31 No. 3 December 2024 Vol. 31 No. 2 (2024): BIOTROPIA Vol. 31 No. 2 August 2024 Vol. 31 No. 1 (2024): BIOTROPIA Vol. 31 No. 1 April 2024 Vol. 30 No. 3 (2023): BIOTROPIA Vol. 30 No. 3 December 2023 Vol. 30 No. 2 (2023): BIOTROPIA Vol. 30 No. 2 August 2023 Vol. 30 No. 1 (2023): BIOTROPIA Vol. 30 No. 1 April 2023 Vol. 29 No. 3 (2022): BIOTROPIA Vol. 29 No. 3 Desember 2022 Vol. 29 No. 2 (2022): BIOTROPIA Vol. 29 No. 2 Agustus 2022 Vol. 29 No. 1 (2022): BIOTROPIA Vol. 29 No. 1 April 2022 Vol. 28 No. 3 (2021): BIOTROPIA Vol. 28 No. 3 December 2021 Vol. 28 No. 2 (2021): BIOTROPIA Vol.28 No.2, Agustus 2021 Vol. 28 No. 1 (2021): BIOTROPIA Vol. 28 No. 1 April 2021 Vol. 27 No. 3 (2020): BIOTROPIA Vol. 27 No. 3 December 2020 Vol. 27 No. 2 (2020): BIOTROPIA Vol. 27 No. 2 August 2020 Vol. 27 No. 1 (2020): BIOTROPIA Vol. 27 No. 1 April 2020 Vol. 26 No. 3 (2019): BIOTROPIA Vol. 26 No. 3 December 2019 Vol. 26 No. 2 (2019): BIOTROPIA Vol. 26 No. 2 August 2019 Vol. 26 No. 1 (2019): BIOTROPIA Vol. 26 No. 1 April 2019 Vol. 25 No. 3 (2018): BIOTROPIA Vol. 25 No. 3 December 2018 Vol. 25 No. 2 (2018): BIOTROPIA Vol. 25 No. 2 August 2018 Vol. 25 No. 1 (2018): BIOTROPIA Vol. 25 No. 1 April 2018 Vol. 24 No. 3 (2017): BIOTROPIA Vol. 24 No. 3 December 2017 Vol. 24 No. 2 (2017): BIOTROPIA Vol. 24 No. 2 August 2017 Vol. 24 No. 1 (2017): BIOTROPIA Vol. 24 No. 1 April 2017 Vol. 23 No. 2 (2016): BIOTROPIA Vol. 23 No. 2 December 2016 Vol. 23 No. 1 (2016): BIOTROPIA Vol. 23 No. 1 June 2016 Vol. 22 No. 2 (2015): BIOTROPIA Vol. 22 No. 2 December 2015 Vol. 22 No. 1 (2015): BIOTROPIA Vol. 22 No. 1 June 2015 Vol. 21 No. 2 (2014): BIOTROPIA Vol. 21 No. 2 December 2014 Vol. 21 No. 1 (2014): BIOTROPIA Vol. 21 No. 1 June 2014 Vol. 20 No. 2 (2013): BIOTROPIA Vol. 20 No. 2 December 2013 Vol. 20 No. 1 (2013): BIOTROPIA Vol. 20 No. 1 June 2013 Vol. 19 No. 2 (2012): BIOTROPIA Vol. 19 No. 2 December 2012 Vol. 19 No. 1 (2012): BIOTROPIA Vol. 19 No. 1 June 2012 Vol. 18 No. 2 (2011): BIOTROPIA Vol. 18 No. 2 December 2011 Vol. 18 No. 1 (2011): BIOTROPIA Vol. 18 No. 1 June 2011 Vol. 17 No. 2 (2010): BIOTROPIA Vol. 17 No. 2 December 2010 Vol. 17 No. 1 (2010): BIOTROPIA Vol. 17 No. 1 June 2010 Vol. 16 No. 2 (2009): BIOTROPIA Vol. 16 No. 2 December 2009 Vol. 16 No. 1 (2009): BIOTROPIA Vol. 16 No. 1 June 2009 Vol. 15 No. 2 (2008): BIOTROPIA Vol. 15 No. 2 December 2008 Vol. 15 No. 1 (2008): BIOTROPIA Vol. 15 No. 1 June 2008 Vol. 14 No. 2 (2007): BIOTROPIA Vol. 14 No. 2 December 2007 Vol. 14 No. 1 (2007): BIOTROPIA Vol. 14 No. 1 June 2007 Vol. 13 No. 2 (2006): BIOTROPIA Vol. 13 No. 2 December 2006 Vol. 13 No. 1 (2006): BIOTROPIA Vol. 13 No. 1 June 2006 No. 25 (2005) No. 24 (2005) No. 23 (2004) No. 22 (2004) No. 21 (2003) No. 20 (2003) No. 19 (2002) No. 18 (2002) No. 17 (2001) No. 16 (2001) No. 15 (2000) No. 14 (1999) No. 13 (1999) No. 12 (1999) No. 11 (1998) No. 10 (1997) No. 9 (1996) No. 8 (1995) No. 7 (1994) No. 6 (1992) No. 5 (1992) No. 4 (1991) No. 3 (1989) No. 2 (1989) Vol. 1 No. 1 (1987) More Issue

Home Page

OAI Link

Editorial Team

Contact

Reviewer

Google Scholar

Contact Name
Knowledge Management Department

Contact Email
kmd@biotrop.org

Phone
+62251-8323848

Journal Mail Official
biotropia@biotrop.org

Editorial Address
SEAMEO BIOTROP Jl. Raya Tajur Km. 6 Bogor 16134 INDONESIA

Location
Kota bogor,

Jawa barat

INDONESIA

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Home Page

OAI Link

Editorial Team

Contact

Reviewer

Google Scholar

Contact Name Knowledge Management Department

Contact Email kmd@biotrop.org

Phone +62251-8323848

Journal Mail Official biotropia@biotrop.org

Editorial Address SEAMEO BIOTROP Jl. Raya Tajur Km. 6 Bogor 16134 INDONESIA

Location Kota bogor, Jawa barat INDONESIA

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Contact Name
Knowledge Management Department

Contact Email
kmd@biotrop.org

Phone
+62251-8323848

Journal Mail Official
biotropia@biotrop.org

Editorial Address
SEAMEO BIOTROP Jl. Raya Tajur Km. 6 Bogor 16134 INDONESIA

Location
Kota bogor,

Jawa barat

INDONESIA