Garuda - Garba Rujukan Digital

Article Per Year (5 Year)

All

The Journal of Experimental Life Sciences (JELS)

jels
Website

Published by Universitas Brawijaya

ISSN : 20872852 EISSN : 23381655 DOI : 10.21776/ub.jels

Core Subject : Health, Science,

Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology Neuroscience

The Journal of Experimental Life Science (JELS) is a scientific journal published by Postgraduate School, University of Brawijaya as distribution media of Indonesian researcher’s results in life science to the wider community. JELS is published in every four months. JELS published scientific papers in review, short report, and articles in Life Sciences especially biology, biotechnology, nanobiology, molecular biology, botany, microbiology, genetics, neuroscience, pharmacology, toxicology, and Applied Life Science including fermentation technology, food science, immunotherapy, proteomics and other fields related to life matter. JELS is a scientific journal that published compatible qualified articles to the academic standard, scientific and all articles reviewed by the expert in their field. The Journal of Experimental Life Science (JELS) have a vision to become qualified reference media to publish the best and original research results and become the foundation of science development through invention and innovation on cellular, molecular, nanobiology, and simulation work related to life matter rapidly to the community. The Journal of Experimental Life Science (JELS) has objectives to published qualified articles on research’s results of Indonesian researchers in life science scope. JELS encompasses articles which discuss basic principles on natural phenomenon with cellular, molecular, and nanobiology approach.

Arjuna Subject : Biokimia, Genetika dan Biologi Molekuler - Biokimia, Genetika dan Biologi Molekuler (Lain-Lain)

Articles 12 Documents

1 2

Search results for , issue "Vol. 10 No. 1 (2020)" : 12 Documents clear

Combination Moringa oleifera Extract and Ifalmin as Potential Formulation of Preventing Inflammation in Diabetic Mice Model Ruri Vivian Nilamsari; Wahyu Isnia Adharini; Noviana Dwi Lestari; Hideo Tsuboi; Muhaimin Rifa'i
The Journal of Experimental Life Science Vol. 10 No. 1 (2020)
Publisher : Postgraduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jels.2019.010.01.07

In Indonesia, the prevalence of diabetes mellitus hits 6.2%, making Indonesia one of the top ten diabetes mellitus countries. Efforts to prevent and treat people with diabetes in Indonesia are required to minimize that as well. One is through treatment with local herbal products such as Moringa oleifera (MO) and Toman fish extract (Channa micropeltes), called Ifalmin. The aim of this research is to investigate the potential role of a combination of Extract Moringa oleifera and Ifalmin to reduce inflammation in diabetes conditions. Diabetic mice were done by Streptozotocin (STZ) induction with a single-dose 145 mg.kg-1.Then, diabetic mice were given an oral treatment of combination MO extract and Ifalmin for 14 days. In this experiment combinations of MO extract and Ifalmin are divide into 3 dose, There are: dose 1 (800 mg.kg-1 : 800 mg.kg-1), dose 2 (650 mg.kg-1 : 650 mg.kg-1), and dose 3 (800 mg.kg-1 : 650 mg.kg-1). Immune cells originate from the spleen are stained by immunofluorescence antibodies and analyzed by flow cytometry with BD Cellquest ProTM software. The results showed an increase of expression pro-inflammatory cytokines IL-1β and IL-6 in diabetic mice compared to normal control. Only dose 1 and dose 2 has shown the capability to reduce the expression of IL-1β in diabetic mice. But, the combination of MO and Ifalmin has an antagonist effect on the expression of IL-6. The inhibitory mechanism can be assumed by the action of antioxidant compounds (Flavonoids and Alkaloids) in MO and Albumin compound in Ifalmin. Those combination act as exogenous antioxidant that help endogenous inside the body. A combination of MO extract and Ifalmin with a certain dosage was able to decrease proinflammatory cytokines IL-1β on the cells involved in innate immunity.

Effect of Colchicine on In Vitro Growth and Ploidicity of Crown Vetiver Plant (Vetiveria zizanioides L. Nash) Anisa Firmandanur Sinta; Wahyu Widoretno
The Journal of Experimental Life Science Vol. 10 No. 1 (2020)
Publisher : Postgraduate School, Universitas Brawijaya

The goal of this research was to evaluate the effect of colchicine on the formation and growth of shoot from explant crowns by in vitro, as well as to obtain polyploidy vetiver plant (Vetiveriazizanioides L. Nash). Induction of polyploidy vetiver plants carried out by culturing explant crowns on MS media supplemented by 2 mg.L-1 BAP and colchicine (0, 30, 60, 90, 120 mg.L-1) for three weeks. Explant and formed shoot regenerated on MS media containing 1 mg.L-1 NAA. The generated plantlets acclimatized on the growing media (cocopeat: husk charcoal: compost = 1: 1: 2). Plant ploidy level of the plants regenerated from treated explant estimated by chromosome counting in root tips. The effect of colchicine on media was able to induce polyploidy in vetiver plants. Five mixoploids were obtained from explant treated colchicine. The vetiver mixoploid plants obtained were 20% and 62.5% from colchicine 60 mg.L-1 and 90 mg.L-1, respectively. The vetiver mixoploid plants consist of diploid (2n=2x=20) and triploid cells (2n=3x=30) or diploid (2n=2x=20) and tetraploid (2n=4x=40). The addition of colchicine in concentrations above 30 mg.L-1 caused inhibition of shoot formation and growth, even a concentration of 120 mg.L-1 caused explant death. However, 30 mg.L-1 colchicine could increase the number of formed shoots, while only 60 mg.L-1 and 90 mg.L-1 could be induced the mixoploidy in the vetiver plant. The results showed that colchicine treatment could increase ploidicity in vetiver plants in vitro, but caused inhibition of shoot formation and growth. Keywords : Chromosome, colchicine, in vitro, polyploid, Vetiveria zizanioides L. Nash.

The Role of Moringa oleifera- Ifalmin® Formulation in Regulation of B220+IgM+ and B220+IgG+ in Diabetic Mice Wahyu Isnia Adharini; Ruri Vivian Nilamsari; Noviana Dwi Lestari; Nashi Widodo; Muhaimin Rifa'i
The Journal of Experimental Life Science Vol. 10 No. 1 (2020)
Publisher : Postgraduate School, Universitas Brawijaya

It has been known that the immunoglobulin levels were altered in diabetes mellitus (DM) conditions. This study aimed to evaluate the levels of immunoglobulins in DM mice after the administration of Moringa oleifera-Ifalmin® formulation (MI). Streptozotocin, at a dose of 145 mg.kg-1, was injected intraperitoneally to experimental mice to obtain diabetic mice. The groups were divided into normal mice, diabetic mice without treatment, diabetic mice with metformin treatment (307.5 mg.kg-1 BW), and diabetic mice with MI treatment at dose 1 (M:I= 800 mg.kg-1 BW: 800 mg.kg-1 BW), dose 2 (M:I= 615 mg.kg-1 BW: 615 mg.kg-1 BW), and dose 3 (M:I= 800 mg.kg-1 BW: 615 mg.kg-1 BW). Mice were orally treated by MI for 14 days. Subsequently, the levels of immunoglobulin IgM and IgG were evaluated using flow cytometry analysis. IgM and IgG levels were significantly lower in the DM group than the normal group. These results indicated that DM altered immunoglobulin levels. MI treatment for 14 days significantly increased the number of IgM and IgG at the level equivalent to the normal group and significantly different as compared to the DM group. Based on the results, MI can be used as an immunomodulatory agent in humoral immunity through the precise regulation of IgM and IgG.

Assessment of Water Quality Using Benthic Macroinvertebrate Along Sumber Maron River, District of Gondanglegi Kulon, East Java-Malang, Indonesia Faiza Tawati; Yenny Risjani; M Sasmito Djati; Bagyo Yanuwiadi
The Journal of Experimental Life Science Vol. 10 No. 1 (2020)
Publisher : Postgraduate School, Universitas Brawijaya

Human activities such as agriculture, swimming, livestock, public washing, and the use of latrines around the river are considered as major causes of damage to the river ecosystem, affecting the growth of Macroinvertebrate, and thus assessing the condition of the river. This study aimed to study the composition of Macroinvertebrate and the quality of water health in Sumber Maron River, in the village of Gondanglegi. This research was conducted from March to April 2018. The method used in this research was the quantitative method using primary data. Research stations were identified by considering land-use in nine regions. The sampling technique of total macroinvertebrates was the kicking technique using the manual mesh with a 500 µm applied to the submerged solid substrates. The data were analyzed using a BMWP Index and interpreted by calculating the Average Scores Per Taxon (ASPT). The result of this research represented that there were 20 taxa of macroinvertebrates, which consisted of It belongs to 8 orders (Trichoptera, Coleoptera, Diptera, Tricladida, Decapoda, Odonata, Ephemeroptera, Plecoptera) and four class (Oligochaeta, Hirudinea, Gastropoda, Crustacea). Generally, the lowest taxa groups found in the station A1 (3 taxa), which were the agriculture and toilet (recreation area). Meanwhile, the highest taxa groups were found in station B3, which was the recreation area, by a total of 14 taxa. Macroinvertebrate with the lowest abundance was Gerridae at station A3, which respectively had the number of 1 ind.5m-2. Macroinvertebrate with the highest abundance was Baetidae at the station B3, which is the recreation area, by the number of 549 ind.5m-2. The result of modified BMWP - ASPT analysis showed a value of 3.3 - 5.75, which indicated that the condition of Sumber Maron River was ranging from bad to excellent category. The bad water condition was found in the agriculture and toilet (recreation area), whereas the excellent water condition was found in the swimming pool (recreation area). Canonical Correspondence Analysis (CCA) revealed that environmental variables were significant for an explanation of the variance in the family. Keywords: BMWP-ASPT, Macroinvertebrate, River.

Physiological and Genetic Variations of Amorphophallus variabilis in Bojonegoro based on Glucomannan Content, Calcium Oxalate and RAPD Markers Achmad Yogi Pambudi; Nunung Harijati; Estri Laras Arumingtyas
The Journal of Experimental Life Science Vol. 10 No. 1 (2020)
Publisher : Postgraduate School, Universitas Brawijaya

This study had purpose to determine the glucomannan and calcium oxalate content in 7 variants of A. variabilis and their genetic relationships based on RAPD markers. Amorphophallus variabilis Tuber samples were taken from Karangdowo village, Sumberrejo Sub-district, Bojonegoro District, East Java. Each variant was analyzed for its glucomannan content by the spectrophotometric method using 3,5-DNS reagents and calcium oxalate by the 0.1N KMnO4 titration method. Leaf DNA extraction was carried out using the CTAB method. Relationship analysis used RAPD markers with 5 primers (OPA-11, OPC-04, OPU-06, OPC-07, and OPN-1). The obtained data were analyzed using the Numerical Taxonomy and Multivariate System (NTSYS-pc) version 2.1. The grouping of glucomannan content or oxalium oxalate used hierarchical clustering analysis (SPSS 16.0). This research found that the calcium oxalate content in seven variants of A. variabilis ranged from 0.01 to 0.03 g, where the variant with the lowest calcium oxalate is V6 with a value of 0.01 g and the highest is the V7 variant with a value of 0.03 g, while the glucomannan content ranges from 9 - 38%. The highest glucomannan content is V3, while the lowest is V6. Phenograms formed based on RAPD markers showed the formation of two groups of A. variabilis. Group one has two subgroups. Subgroup one consists of variants V1 and V4, while subgroup two consists of V6 and V7. Meanwhile, the second group consists of variants V2, V3, and V5. The seven variant grouping pattern of A. variabilis based on RAPD markers has no similarity to the grouping pattern based on the results of glucomannan or calcium oxalate analysis.

Effect of Benzyl Adenine Concentration on Callus Induction of Geranium Plants (Pelargonium graveolens L'Her) from Petiole and Leaf Explants Moch Faizul Huda; Serafinah Indriyani; Wahyu Widoretno
The Journal of Experimental Life Science Vol. 10 No. 1 (2020)
Publisher : Postgraduate School, Universitas Brawijaya

Geranium plant (Pelargonium graveolens L'Her) is one of the geranium oil producing plants that has many benefits. Callus culture is a technique that can be used to plant multiplication and increase production of secondary metabolites. This study aims to determine the effect of the concentration of Benzyl Adenine on the formation of geranium callus from petiole and leaf explants. Callus induction was carried out by culturing petiole and leaf explants on MS medium + 0.1 mg.L-1 NAA + Benzyl Adenine (0; 0.5; 1; 1.5 and 2 mg.L-1). Callus morphological parameters, percentage of callus formation, and time of first callus formation were observed. The formation of geranium callus influenced by the explant type and the concentration of Benzyl Adenine. In the 2nd week, the geranium callus was initiated, light green colored with a compact callus texture. At 4th week, the percentage of callus formation containing NAA 0.1 mg.L-1 of petiole and leaf explants was 20% and 8%, whereas the percentage of callus formation on medium containing 0.1 mg.L-1 NAA combined with 0.5-2 mg.L-1 Benzyl Adenine of petiole and leaf explants was 52-80% and 24-52%. The best percentage of callus formation was found on the culture medium containing 1 mg.L-1 Benzyl Adenine, equaled 80% of petiole explants, and 52% of leaf explants. Keywords: BA, Callus, Leaf, Petiole, Pelargonium graveolens L'Her.

Repairing Cell Structure of Jejunum Tissue in RA-CFA Rat Model Improved by Caprine CSN1S2 Protein Laili Nur Zaidah; Aris Soewondo; Fatchiyah Fatchiyah
The Journal of Experimental Life Science Vol. 10 No. 1 (2020)
Publisher : Postgraduate School, Universitas Brawijaya

We aimed to analyze the effect of CSN1S2 protein in Etawah crossbred goat milk and yogurt on the histopathology of the jejunum and the amount of cell damage in Complete Freund's adjuvant (CFA)-induced rheumatoid arthritis (RA) rats. The rats were divided into six groups: the untreated control rats (C), control rats were given CSN1S2 protein from Etawah crossbred goat milk (CM) or yogurt (CY), RA rats, RA rats given CSN1S2 protein from Etawah crossbred goat milk (RAM) or yogurt (RAY). Hematoxylin-eosin staining was conducted for the histopathological analysis of jejunum. Statistical analysis was done using one-way ANOVA (a significance value of p ≤ 0.05) followed by the Tukey test. Our study observed that the control, CM, and CY group have a normal histological structure of jejunum. The damage to the jejunum structure was reported in the RA group. The milk CSN1S2 protein was able to improve the structure of jejunum villi and increase the normal cell number in the jejunum of the RA group, similar to control. The RAY group showed an impaired jejunum structure and a high number of necrotic cells as in the RA group.

Detection of Reproductive Status in Ongole Crossbred (PO) Cow Based On Vaginal Epithel Morphology and Profile Hormone Yeni Widyaningrum; Aulanni'am Aulanni'am; Agung Pramana Warih Marhendra
The Journal of Experimental Life Science Vol. 10 No. 1 (2020)
Publisher : Postgraduate School, Universitas Brawijaya

Hormonal fluctuations in livestock will affect vaginal cytology good overview on the condition of estrous until pregnancy. The purpose of this study was to determine the physiological condition of Ongole crossbred (PO) cow during estrous and determine pregnancy by the description of vaginal epithelial cells, progesterone, and estrogen hormone profiles. The materials were used 35 cows with physiological status (estrous, 5th pregnancy period, 16th pregnancy period, 22nd pregnancy period, and 60th pregnancy period). Samples of Vaginal smear were stained with Giemsa, then it was observed using a microscope, with 40 times magnification. The progesterone and estrogen were analyzed by the ELISA method. The parameters measured were the percentage of vaginal epithelial cells, such as (parabasal, intermediate, and superficial) started estrous phase until the time of pregnancy in cows (5, 16, 22, and 60 days), hormone concentration, as well as the presence or absence of leukocytes. The result showed the Ongole crossbred cow estrous phase percentage of superficial cells 56.27%±6.49 higher than 26.23%±7.98 intermediate cells, followed by parabasal cells 17.50%±4.74. While in Ongole crossbred that were 5th pregnancy period until the 60th predominantly intermediate cell 80.43%±1.31, then the superficial cells 18.09%±1.30 and 1.48%±0.04 parabasal cells. Progesterone concentration was 63.74±1.07 ng.mL-1 in estrus cows, and steadily increased 93.71±0.94 ng.mL-1 to 149.5±0.71 ng.mL-1 in pregnant cows (5-60 days). The concentration of high estrogen levels were 122.38±0.63 ng.mL-1 in the estrous phase, then decreased 81.54±0.44 ng.mL-1 in the pregnancy phase. In conclusion, the concentration of hormone showed a diagnosis of pregnancy, which done by looking at changes in vaginal epithelial cells at the Ongole crossbred cow, and the cow estrous phase showed greater superficial cells compared by pregnant cows (5-60 days). Keywords: diagnosis of pregnancy, estrous, hormone, Ongole crossbred of cow, vaginal cytology.

The Effectiveness of Combination of Momordica charantia Extract and Averrhoa bilimbi on Nf-Kb Activation in Mice (Mus musculus) Balb/C Hyperglycemia Models Intan Sartika Risky Sampurna; Muhaimin Rifa'i; Sri Rahayu
The Journal of Experimental Life Science Vol. 10 No. 1 (2020)
Publisher : Postgraduate School, Universitas Brawijaya

Hyperglycemia is a medical condition when the blood glucose levels increase exceeds the reasonable limit. Hyperglycemia is a typical sign of diabetes mellitus (DM). Indonesia is the sixth-ranked country in the world after China, India, United States, Brazil, and Mexico. Treatment with herbs is currently being developed. Pare (Momordica charantia) and Belimbing wuluh (Averrhoa bilimbi) are plants that found around us. Some studies state that each of these plants can be anti-diabetic. The hyperglycemia can cause an immune system disorder characterized by pancreatic β cell death involving IL-1β, kappa B (NF)-κB nuclear factor, and Fas. The ability of NF-kB activation will affect the number of cytokines expressed by T cells, namely TNF-α, and IFN-γ. The purpose of this study is to determine the effect of NF-kB activation on blood glucose levels in hyperglycemia mice. The results showed that the positive control treatment showed an increase in the number of NF-kB activations in CD4 and CD8 cells. EPBW (combination of Averrhoa bilimbi extract and Momordica charantia) administration results at a dose of 10 mg.kg-1 BW showed a reduction in the amount of activated NF-kB as a substitute for the reduction. In addition, that dose can reduce blood sugar levels in mice hyperglycemia model.

The Artificial Bee Colony (ABC) Algorithm for Estimating Parameter of Epidemic Influenza Model Ririn Nirmalasari; Agus Suryanto; Syaiful Anam
The Journal of Experimental Life Science Vol. 10 No. 1 (2020)
Publisher : Postgraduate School, Universitas Brawijaya

The Artificial Bee Colony (ABC) is one of the stochastic algorithms that can be applied to solve many real-world optimization problems. In this paper, The ABC algorithm was used to estimate the parameter of the epidemic influenza model. This model consists of a differential system represented by variations of Susceptible (S), Exposed (E), Recovered (R), and Infected (I). The ABC processes explore the minimum value of the mean square error function in the current iteration to estimate the unknown parameters of the model. Estimating parameters were made using participation data containing influenza disease in Australia, 2017. The best parameter chosen from the ABC process matched the dynamical behavior of the influenza epidemic field data used. Graphical analysis was used to validate the model. The result shows that the ABC algorithm is efficient for estimating the parameter of the epidemic influenza model. Keywords: ABC, Epidemic, Estimate, Influenza, Parameter.

Page 1 of 2 | Total Record : 12

1 2

Filter by Year

2020 2020

Filter By Issues

All Issue Vol. 15 No. 3 (2025) Vol. 15 No. 2 (2025) Vol. 15 No. 1 (2025) Vol. 14 No. 3 (2024) Vol. 14 No. 2 (2024) Vol. 14 No. 1 (2024) Vol. 13 No. 3 (2023) Vol. 13 No. 2 (2023) Vol. 13 No. 1 (2023) Vol. 12 No. 3 (2022) Vol. 12 No. 2 (2022) Vol. 12 No. 1 (2022) Vol. 11 No. 3 (2021) Vol. 11 No. 2 (2021) Vol. 11 No. 1 (2021) Vol. 10 No. 3 (2020) Vol. 10 No. 2 (2020) Vol. 10 No. 1 (2020) Vol. 9 No. 3 (2019) Vol. 9 No. 2 (2019) Vol. 9 No. 1 (2019) Vol. 8 No. 3 (2018) Vol. 8 No. 2 (2018) Vol. 8 No. 1 (2018) Vol. 7 No. 2 (2017) Vol. 7 No. 1 (2017) Vol. 6 No. 2 (2016) Vol. 6 No. 1 (2016) Vol. 5 No. 2 (2015) Vol. 5 No. 1 (2015) Vol. 4 No. 2 (2014) Vol. 4 No. 1 (2014) Vol. 3 No. 2 (2013) Vol. 3 No. 1 (2013) Vol. 2 No. 2 (2012) Vol. 2 No. 1 (2012) Vol. 1 No. 2 (2011) Vol. 1 No. 1 (2011) More Issue

Home Page

OAI Link

Editorial Team

Contact

Reviewer

Google Scholar

Contact Name
Jehan Ramdani Hariyati

Contact Email
jehanramdani@ub.ac.id

Phone
+6282333752235

Journal Mail Official
jehanramdani@ub.ac.id

Editorial Address
Jl. Veteran Malang 65145

Location
Kota malang,

Jawa timur

INDONESIA

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Home Page

OAI Link

Editorial Team

Contact

Reviewer

Google Scholar

Contact Name Jehan Ramdani Hariyati

Contact Email jehanramdani@ub.ac.id

Phone +6282333752235

Journal Mail Official jehanramdani@ub.ac.id

Editorial Address Jl. Veteran Malang 65145

Location Kota malang, Jawa timur INDONESIA

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Contact Name
Jehan Ramdani Hariyati

Contact Email
jehanramdani@ub.ac.id

Phone
+6282333752235

Journal Mail Official
jehanramdani@ub.ac.id

Editorial Address
Jl. Veteran Malang 65145

Location
Kota malang,

Jawa timur

INDONESIA