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SIMBIOSIS

simbiosis
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Published by Universitas Udayana

ISSN : 23377224 EISSN : 26567784 DOI : 10.24843/simbiosis

Core Subject : Agriculture,

Agriculture, Biological Sciences & Forestry

Arjuna Subject : -

Articles 5 Documents

Search results for , issue "Vol 7 No 2 (2019)" : 5 Documents clear

BIOKONTROL ENDOMYCORRHIZA AGAINST FUNGUS Curvularia sp. THE CAUSE OF DISEASE ON CORN (Zea mays L.) Lily Windasari; Meitini Wahyuni Proborini; Made Ria Defiani
SIMBIOSIS Vol 7 No 2 (2019)
Publisher : Jurusan Biologi Universitas Udayana

In Indonesia, production of corn are used for fodder (55%), food (30%), and seed (15%). The growth of corn plants is limited by the presence of pests, weeds, and plant diseases. Curvularia sp. causing leaf spot and could decrease corn production. Use of mycorrhiza can be used as a biological agent in preventing pathogens outbreaks. Isolates of Curvularia sp. fungal was sprayed on corn seed. This research was conducted in laboratorium and green house. Corn seedlings were grown on sterile soil media (control), mycorrhizal treatments (100g of mycorrhizal + Curvularia sp., 200g of mycorrhizal + Curvularia sp., 300g of mycorrhizal + Curvularia sp.) and Curvularia sp. The observed variables were plant height, number of leaves, rate of disease progression, fresh weight and dry weight of plant canopy. The results showed that application of 100g inoculum tend to increase plant growth to 26-27% and percentage of mycorrhizal colonization to 58,66% thereof can decrease pathogen infection on plant at 28 day after planting.

INVESTIGATING THE GLOBAL WARMING/CLIMATE CHANGE (LA-NINA) PHENOMENA, LAND USE CHANGE AND ENVIRONMENTAL DEGRADATION MITIGATION ON BALI ISLAND I Wayan Kasa; Ida Bagus Wayan Gunam
SIMBIOSIS Vol 7 No 2 (2019)
Publisher : Jurusan Biologi Universitas Udayana

Up to now Bali island still suffering from light to heavy rainfall (August 2016), eventhough it must be hot season as usual (April to October). Such situation is recognised as La-Nina phenomenon. Methode empolyed in this study is collecting primary (direct field check) and scondary data (literature study). Results showed that, the La-Nina phenomena most affected coffee plantation farmers in the village of Sepang, district of Busungbiu, Buleleng regency. The coffee fruit fall out from stem branch prior to harvest time and becoming black in colour. In addition, some coffee fruit skin are white in colour in comparison to red to black during normal hot season. Land use change could also be seen over Bali. Increase sea level on some beaches are also another disaster around Bali. Such accidence was significantly happened on the beach of Lepang village, district of Banjarangkan, Klungkung regency. It was found that such increases puddling area of more or less five hectare of paddy rice field on that area. The global warming/climate change phenomenon also cause habitat loss of specific ancient crab who live in Nusa Penida beach and move into the Giri Putri holy cave to survive. However, the only government do to compromise and mitigate such abnormal environmental degradation would be by strengthening the essen of the Eka Karya botanical garden of Bedugul as an open green field area for refreshing.

INVENTORY OF WILD PLANT TYPE OF TRADITIONAL MEDICINE MADURA SUMENEP COMMUNITIES Ismawati Ismawati; R. Amilia Destryana
SIMBIOSIS Vol 7 No 2 (2019)
Publisher : Jurusan Biologi Universitas Udayana

Sumenep is one of the regencies on Madura Island which is famous for traditional herbal medicine. The traditional herbal medicine ingredients of the Sumenep community come from wild plants and cultivation plants. The research aims to identify wild plant species as an effort to inventory wild plants that are used as traditional herbal ingredients. The research is an exploratory study conducted by in-depth interviews with sources who understand traditional herbal medicine such as traditional birth attendants, masseurs and herbalists. The study was conducted in District of Dungkek, Batang-Batang and Gapura. The results found 22 families of wild plants consisting of 29 types of plants with diverse benefits as ingredients of traditional herbal medicine.

IN SILICO DNA PROBE DESIGN FOR MUTATION DETECTION OF QUINOLON RESISTANCE AREA gyrA AND gyrB GENE Mycobacterium tuberculosis Tasya Pramiswari; Jennifer Tamara; Ni Made Febrianti; Sagung Chandra Yowani
SIMBIOSIS Vol 7 No 2 (2019)
Publisher : Jurusan Biologi Universitas Udayana

Fluoroquinolone (FQ) is the main drug used in MDR-TB therapy resistance to FQ can cause death and increase the risk of treatment failure in MDR-TB patients. Mutations in gyrA gene and gyrB gene from Mycobacterium tuberculosis are responsible for the occurrence of FQ resistance. The highest mutation of gyrA gene in QRDR was found in codon 94, while mutations in gyrB gene was found in codon 500. M. Tuberculosis which resistant to FQ can be detected using the Real Time Polymerase Chain Reaction (RT-PCR) method with DNA probe. This study will design the nucleotide sequence of the TaqMan type probe using the Clone Manager Suite 9.2 program. The results of the DNA probe design were then analyzed in two stages, which is based on the probe criteria in general and based on the TaqMan probe labeling criteria. The design of the mutant probe DNA using the program produced 1 probe for Asp94Ala specific mutations in the gyrA gene and 33 probes for Asp500Ala specific mutations in the gyrB gene. After being analyzed by the two criteria, it was obtained the A94MA1 probe with the 5 '-TCGATCTACGCCAGCCTGGT-3' sequence and B500MA12 probe with the order of 5 '-TACCACAAGCTCGTGCTGATGGC-3'. The results of these probes meet both criteria and can be used to detect mutations in codon 94 gyrA genes and codons 500 gyrB genes of Mycobacterium tuberculosis.

DIVERSITY AND MANAGEMENT OF PLANTS IN STAR HOTEL AT UBUD TOURISM AREA Sri Afrianingsih; I Ketut Ginantra; I Ketut Muksin
SIMBIOSIS Vol 7 No 2 (2019)
Publisher : Jurusan Biologi Universitas Udayana

This research is aimed to determine plants species in 3 hotels, by grouping them into rare plants, medicinal plants, water-saving plants and how to manage them. The sample was obtained by a simple random sampling technique on the entire starred hotels in Ubud. The hotels which are the object of research are the Four Seasons, Maya Ubud, and The Royal Pita Maha. Data collection is done by doing observation, literature study, document checking and interviews. The type of data used is quantitative and qualitative data with the types of data sources are primary and secondary. Data analysis which used in this study are descriptive qualitative and quantitative. The results of this research in Four Seasons hotel are found 38 plants species from 24 families which 17 species grouped into rare / protected plants, 14 medicinal plants species and 15 water-saving plants species. Next, in the Maya Ubud hotel found 48 plants species from 27 families which grouped into 26 species of rare / protected plants, 15 medicinal plants species and 18 water-saving plants. The last, at The Royal Pita Maha hotel found 45 plants species from 24 families which grouped into 27 rare / protected plants species , 8 medicinal plants species and 21 water-saving plants. Plants management method of those hotels is loose ground, selecting superior seeds, cleaning weeds, using organic fertilizers, pest controlling, and arranging watering schedules.

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Journal Mail Official
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Kota denpasar,

Bali

INDONESIA