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AL KAUNIYAH
ISSN : 19783736     EISSN : 25026720     DOI : 10.15408/kauniyah
Core Subject : Science,
Al-Kauniyah: Jurnal Biologi (p-ISSN: 1978-3736, e-ISSN: 2502-6720) is an Open Access Journal published by Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islamic University Jakarta, and established since 2007. Since 2016 Al-Kauniyah has established a collaboration with the Association of Lecturer in Biology and Biology Education throughout the State Islamic Higher University (PTKIN) in Indonesia. Until 2015, Al-Kauniyah covered environmental biology solely, but since 2016 the journal has been extended to cover the entire field of biological science (bioscience). By publishing biannually, on April and October, Al-Kauniyah is intended to communicate original researches and current issues on the subject of biology. Since volume 9 issue 1 April 2016, Al-Kauniyah had been changes the layout. This journal warmly welcomes contributions from scholars of related disciplines. Manuscripts can be submitted to AL-KAUNIYAH
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Articles 460 Documents
ISOLASI Escherichia coli DARI URINE PASIEN INFEKSI SALURAN KEMIH DI RUMAH SAKIT BHAYANGKARA KEDIRI Mastuti Widianingsih; Aldino Marcos de Jesus
Al-Kauniyah: Jurnal Biologi Vol 11, No 2 (2018): Al-Kauniyah Jurnal Biologi
Publisher : Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islami

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (980.365 KB) | DOI: 10.15408/kauniyah.v11i2.5899

Abstract

AbstrakInfeksi saluran kemih (ISK) terjadi akibat adanya invasi mikroorganisme (bakteri) pada saluran kemih. Peningkatan kejadian ISK dapat dipengaruhi oleh kondisi refluks vesikouretral (RVU), obstruksi saluran kemih, pemakaian instrumen uretral baru, dan septikimia. Angka kejadian ISK di Rumah Sakit Bhayangkara Kediri tahun 2016 berjumlah 346 kasus. Penelitian ini bertujuan untuk mengetahui ada tidaknya Escherichia coli pada urine pasien ISK di Rumah Sakit Bhayangkara Kediri. Besarnya persentase ISK yang disebabkan E. coli mendorong peneliti untuk melakukan isolasi bakteri tersebut dari urine pasien ISK di rumah sakit tersebut. Teknik sampling yang digunakan berupa Accidental sampling dengan sampel berupa urine porsi tengah (UPT) sebanyak 30. Sampel urine diinokulasikan pada media MCA, kemudian dilakukan pewarnaan Gram, dilanjutkan uji biokimia reaksi untuk membedakan golongan Enterobacter. Hasil penelitian menunjukkan bahwa 12 sampel positif mengandung E. coli, 3 sampel mengandung Klebsiella spp., dan 15 sampel tidak terdeteksi sama sekali. Hasil positif E. coli ditunjukkan dengan koloni bulat berukuran kecil, elevasi semi mucoid, dan fermentasi laktosa positif pada media MCA. Hal tersebut menunjukkan bahwa E. coli dapat ditemukan pada sampel urine pasien ISK di Rumah Sakit Bayangkara Kediri.Abstract Urinary tract infection (UTI) is caused by microbial invasion (bacteria) in the urinary tract. The increased of UTI can be affected by a condition of vesicouretral reflux (RVU), urinary tract obstruction, application of new urethral instruments, and septicemia. The incidence of UTI in Bhayangkara Kediri Hospital in 2016 was 346 cases. The objective of this research was to determine the presence of E. coli in UTI patients in the Bhayangkara Kediri Hospital. The large percentage of UTI caused by Escherichia coli encouraged researchers to isolate the bacteria from the urine of UTI patients in the hospital. Accidental sampling with 30 middle portion urine samples (UPT) was carried out. The samples were inoculated onto separate MCA media. Representative bacterial isolates were stained with Gram staining technique and followed by reaction biochemistry tests to distinguish Enterobacter groups. The results showed that 12 urine samples contained E. coli, 3 urine samples contained Klebsiella spp., while 15 urine samples were negative (not containing bacteria). The positive results of E. coli showed small rounded, elevation of semi mucoid colonies, and positive lactose fermentation on the MCA media. It showed that E. coli indeed exists in the urine samples of UTI patients in Bayangkara Kediri Hospital.
ISOLASI BAKTERI HETEROTROF DI SITU CIBUNTU, JAWA BARAT DAN KARAKTERISASI RESISTENSI ASAM DAN LOGAM Kesi Kurnia
Al-Kauniyah: Jurnal Biologi Vol 9, No 2 (2016): Al-Kauniyah Jurnal Biologi
Publisher : Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islami

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (364.499 KB) | DOI: 10.15408/kauniyah.v9i2.3080

Abstract

AbstrakBakteri heterotrofik sangat berperan penting dalam sistem perairan karena kemampuan aktivitas metabolismenya. Bakteri tersebut berinteraksi dengan logam dan mineral dalam lingkungan alam dan sintetis, mengubah keadaan fisik dan kimianya sehingga mempengaruhi aktivitas, pertumbuhan dan kelangsungan hidup bakteri. Penelitian ini bertujuan untuk mengisolasi dan mengkarakterisasi bakteri resisten logam timbal (Pb) dan asam di Situ Cibintu, Cibinong, Jawa Barat. Sampel air diambil dari 3 titik di Situ Cibuntu dan dianalisis distribusi bakteri heterotrofiknya. Isolat bakteri dikultur di media Triptone Glucose Yeast (TGY). Selanjutnya dilakukan resistensi terhadap logam timbal (Pb) dan asam. Hasil penelitian menunjukkan bahwa jumlah bakteri heterotrof di Situ Cibuntu rata-rata 3,5x103 cfu m/L. Mayoritas (73%) isolat bakteri resisten terhadap logam timbal sebanyak 47% dan resisten terhadap asam (pH 4). Bakteri yang resisten didominasi oleh Gram negatif. Penelitian ini memberikan pengetahuan mengenai kemampuan adaptasi bakteri heterotrof pada lingkungan logam dan asam.Abstract Heterotrophic bacteria play an important role in aquatic systems because of their ability of metabolic activities. They interact with metals and minerals in natural and synthetic environments, altering their physical and chemical state, with metals and minerals also able to affect activity, microbial growth and survival. The purpose of study is to isolate and characterize the lead (Pb) and acid resistant of heterotrophic bacteria strains isolated from pond Cibuntu, Cibinong, West Java. Water samples were collected from three points around pond Cibuntu and analyzed the distribution of heterotrophic bacteria. The bacterial isolates were screened on Triptone Glucose Yeast (TGY) agar plates. Lead (Pb) metal and acidic tolerance were tested. In the present study, the population density of heterotrophic bacteria were recorded 3.5x103 cfu m/L. The majority (73%) of bacteria isolates were resistant to lead and 47% to acid (pH 4). The most abundant type of bacteria resistant is Gram negative. This experiment provides the base to link the adaptation capabilities of heterotrophic bacteria in metal and acid environment.Permalink/DOI: http://dx.doi.org/10.15408/kauniyah.v9i2.3080
Perbandingan Pertumbuhan Jamur Tiram Putih di Kumbung Ciseeng dan Universitas Al-Azhar Indonesia Putri Kenanga; Arief Pambudi; Riris L. Puspitasari
Al-Kauniyah: Jurnal Biologi Vol 7, No 2 (2014): Al-Kauniyah Jurnal Biologi
Publisher : Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islami

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (221.263 KB) | DOI: 10.15408/kauniyah.v7i2.2721

Abstract

White oyster mushroom (Pleurotus ostreatus) is one of wood fungi which easy to grow at rotting wood and organic waste. White oyster mushroom is rich in protein, carbohydrates, minerals, and vitamins. Environmental factors affecting the growth of white oyster mushrooms is temperature and humidity. The study aims to compare the growth and production of white oyster mushroom in different location, Ciseeng and University of Al-Azhar Indonesia (UAI). Cultivation in UAI done in a controlled situation using autoclave sterilization, inoculation using enkas and the treatment is watering on gunny sack in the mushroom house regularly. Whereas, cultivation in Ciseeng less controlled by traditional cultivation using steam sterilization and inoculation process at open space. Wet weight, dry weight, and biological efficiency results is higher in UAI than Ciseeng at 7.95%, 14.5%, and 7.34% higher, respectively. Morphological parameter such as number of fruiting body, mushroom cap diameters, and stem lenght also show better in UAI than Ciseeng at 23.67%, 8.75%, 54.96% higher, respectively. Better result of mushroom cultivation at UAI supported by 28.3oC average temperature and 93.95% humidity, meanwhile in Ciseeng average temperature and humidity was 29.25oC and 82.15%.
AKTIVITAS ANTIFUNGI ISOLAT AKTINOMISETES ARBORETUM UNIVERSITAS RIAU TERHADAP JAMUR Fusarium oxysporum f.sp lycopersici DAN Ganoderma boninense Vista Queendy; Rodesia Mustika Roza
Al-Kauniyah: Jurnal Biologi Vol 12, No 1 (2019): Al-Kauniyah Jurnal Biologi
Publisher : Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islami

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (797.302 KB) | DOI: 10.15408/kauniyah.v12i1.8793

Abstract

AbstrakRendahnya produktivitas lahan pertanian di Indonesia berkaitan dengan serangan penyakit tanaman, contohnya penyakit layu Fusarium pada tanaman tomat dan busuk pangkal batang pada tanaman kelapa sawit yang disebabkan oleh Ganoderma boninense. Penelitian sebelumnya telah berhasil mengisolasi aktinomisetes dari tanah Arboretum Universitas Riau, namun belum diketahui kemampuannya dalam menghambat jamur Fusarium oxysporum f.sp lycopersici dan Ganoderma boninense. Penelitian ini bertujuan untuk menguji 32 isolat aktinomisetes terhadap jamur target dengan metode difusi agar. Aktinomisetes potensial yang terpilih, dilanjutkan untuk produksi senyawa antifungi dengan lama waktu fermentasi yang berbeda (4, 5, dan 6 hari) dan diuji dengan metode difusi kertas cakram. Hasil penelitian uji aktivitas antifungi dengan metode difusi agar menunjukkan 31 isolat aktinomisetes mampu menghambat pertumbuhan jamur F. oxysporum f.sp lycopersici dan isolat A2.01 memiliki zona hambat tertinggi yaitu sebesar 36,10 mm.  Sebanyak 28 isolat aktinomisetes mampu menghambat jamur G. boninense dan isolat D2.28 memiliki zona hambat tertinggi, yaitu sebesar 45,71 mm. Isolat aktinomisetes Arboretum Universitas Riau sebagian besar dapat menghambat pertumbuhan kedua jamur target dengan metode difusi agar, namun hasil uji senyawa antifungi isolat potensial dengan metode difusi kertas cakram belum mampu membentuk zona hambat. Isolat aktinomisetes potensial dapat diaplikasikan menggunakan kultur langsung secara in vivo.Abstract The low productivity of agricultural land in Indonesia occurred because of plant diseases attack such as Wilt Fusarium in tomato plant and Basal Stem Rot disease in palm oil caused by Ganoderma boninese. Previous research had successfully isolated the actinomycetes from soil of Arboretum University of Riau However, the antifungal potency of those isolates against Fusarium oxysporum f. sp lycopersici and Ganoderma boninense had not been known. This research aimed to screening 32 actinomycetes isolates against the fungal target by using agar diffusion method. The selected actinomycetes were continued to produce crude antifungal by different duration of fermentation (4, 5 and 6 days) using disc diffusion method.  The result of the antifungal activity using agar diffusion method showed that there were 31 isolates that able to inhibit the F. oxysporum f. sp lycopersici and isolate A2.01 showed the highest inhibition zone by 36.10 mm. There were 28 isolates that able to inhibit the G. boninense and isolate D2.28 showed the highest inhibition zone by 45.71 mm.  Almost all of the isolate was able to inhibit both of two fungals targets with diffussion agar method, but the crude antifungal compound of the potential isolate by disc diffusion method could not inhibit both of the fungal targets. The potential actinomycetes isolate was suggested to be applied as a culture directly in vivo.
Daya Hambat Perak Nitrat (Agno3) pada Perkecambahan Biji Kacang Hijau (Vigna radiata) Muh. Shofi
Al-Kauniyah: Jurnal Biologi Vol 10, No 2 (2017): Al-Kauniyah Jurnal Biologi
Publisher : Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islami

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (590.188 KB) | DOI: 10.15408/kauniyah.v10i2.4869

Abstract

Abstrak Logam berat banyak digunakan dalam kehidupan manusia, di antaranya Hg, Pb, Cr, Zn, dan Ag. Di antara logam tersebut, logam perak (Ag) banyak digunakan oleh masyarakat untuk kegiatan sehari-hari, seperti dalam fotografi, untuk pembuatan cermin perak, dan sebagai reagen dalam analisis. Logam perak dapat diperoleh dari senyawa AgNO3. Keberadaan logam perak pada tanaman dapat menghambat proses perkecambahan pada tanaman kacang hijau (Vigna radiata), yang ditandai dengan penghambatan pemanjangan sel pada akar. Tujuan penelitian ini adalah untuk mengetahui pengaruh daya hambat terhadap pertumbuhan kecambah kacang hijau dan mengetahui konsentrasi hambatan (Inhibitory Concentration) dari perak nitrat (AgNO3). Berdasarkan hasil penelitian diketahui bahwa terdapat penghambatan pada pembentukan akar dengan rata-rata penghambatan lebih dari 50% pada konsentrasi 462,27 ppm. Hal ditunjukkan pada panjang akar yang lebih pendek seiring dengan tingginya konsentrasi AgNO3. Hal tersebut dapat disimpulkan bahwa konsentrasi AgNO3 berpengaruh pada perkecambahan biji kacang hijau yang ditandai dengan terhambatnya pemanjangan panjang akar kacang hijau.Abstract Heavy metals are widely used in human life, including Hg, Pb, Cr, Zn, and Ag. Among these metals, silver is widely used for human daily activities, such as in photography, for the manufacture of silver mirror, and as reagents in many analysis. Silver metal can be obtained from AgNO3 compounds. The presence of silver metal in a plant may inhibit the germination process in the green bean plant (Vigna radiata) which is characterized by inhibition of cell lengthening in the root. The aims of this research are to investigate the influence of the inhibitory power to green bean growth and the inhibitory concentration of the silver nitrate (AgNO3). The result showed that there was inhibition that occurred in the root formation by more than 50% of average inhibition at the concentration of 462.27 ppm. It was shown that the root was shorter in length along with the high concentration of AgNO3. From that fenomena, it can be assumed that the concentration of AgNO3 influenced the germination of green bean seeds that were characterized by the inhibition on the lengthening of their roots.
CATATAN TENTANG Solanum diphyllum L. (SOLANACEAE) TERNATURALISASI DI PULAU JAWA Muhammad Rifqi Hariri; Arifin Surya Dwipa Irsyam
Al-Kauniyah: Jurnal Biologi Vol 11, No 1 (2018): Al-Kauniyah Jurnal Biologi
Publisher : Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islami

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (834.869 KB) | DOI: 10.15408/kauniyah.v11i1.5448

Abstract

Abstrak Solanum merupakan salah satu marga terbesar pada kelompok tumbuhan Angiospermae. Marga tersebut terdiri dari 1400 jenis yang terdistribusi di kawasan tropis dan subtropis. Banyak anggotanya yang memiliki nilai ekonomi tinggi, sehingga telah dibudidayakan secara luas sebagai tanaman pangan, sayuran, tumbuhan obat, dan tanaman hias. Oleh sebab itu, beberapa di antaranya telah diintroduksi ke Pulau Jawa sejak lama. Sebanyak 24 jenis Solanum telah tercatat dalam buku Flora of Java vol. 2. Meskipun demikian, masih memungkinkan adanya jenis-jenis lain yang belum terekam dalam buku tersebut. Penelitian ini bertujuan untuk memberikan informasi mengenai jenis tambahan yang telah ternaturalisasi di Pulau Jawa. Pengamatan dilakukan di beberapa lokasi di Jawa Barat, Jawa Tengah, dan Jawa Timur pada tahun 2015 hingga 2017. Solanum diphyllum telah dikoleksi dari Bogor (Darmaga), Bandung (Taman Sari dan Pasir Impun), Sumedang (Jatinangor), Wonogiri (Desa Johunut), Semarang (Desa Mesu), Trenggalek (Desa Watulimo), Situbondo (Desa Wringin Anom dan Banyuputih), Bondowoso (Desa Bandilan), dan Sumenep (Desa Pakong). Jenis ini memiliki potensi untuk dimanfaatkan sebagai tumbuhan obat. Tumbuhan ini ditemukan tumbuh meliar di semua lokasi pengamatan dan keberadaannya perlu diperhatikan karena jenis tersebut merupakan tumbuhan asing invasif di kawasan lain.  Abstract Solanum is one of the largest genera in Angiospermae. This genus comprises about 1400 species distributed in tropic and subtropic regions. Some of them have high economic value and have been cultivated widely as crops, vegetables, medicinal plants, and ornamental plants. Therefore, several species have been introduced to Java island in the past. A total of 24 species of Solanum has been recorded in Flora of Java vol. 2. However, there is still unrecorded species occurred. The aim of this study was to provide information about an additional naturalized species in Java. The observations have been carried out in several locations in West Java, Central Java, and East Java, from 2015 to 2017. Solanum diphyllum has been collected from Bogor (Darmaga), Bandung (Taman Sari and Pasir Impun), Sumedang (Jatinangor), Wonogiri (Johunut Village), Semarang (Mesu Village), Trenggalek (Watulimo Village), Situbondo (Wringin Anom and Banyuputih Village), Bondowoso (Bandilan Village), and Pamekasan (Pakong Village). This species has a potential to be utilized as a medicinal plant. Moreover, this plant grows wildly in all of the observed locations. Its presence needs to be considered since it is known as an invasive alien species in other regions.  Permalink/DOI: http://dx.doi.org/10.15408/kauniyah.v11i1.5448 
Estimasi Karbon Tersimpan pada Tegakan Pohon di Hutan Pantai Pulau Kotok Besar, Bagian Barat, Kepulauan Seribu Mohamad Fazri Hikmatyar; Tubagus Muhammad Ishak; Ario Putro Pamungkas; Sayyidah Sofie Annisa Mujahidah; Alfan Farhan Rijaludin
Al-Kauniyah: Jurnal Biologi Vol 8, No 1 (2015): Al-Kauniyah Jurnal Biologi
Publisher : Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islami

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (181.019 KB) | DOI: 10.15408/kauniyah.v8i1.2704

Abstract

The high activity of urban communities anthropogenic especially in Jakarta City cause high rates of carbon emissions. This problem is increased with the high rate of deforestation which is high enough in urban areas. One of ecosystem that can reduce carbon emissions is coastal forest ecosystem. Forests can reduce carbon emissions in the atmosphere and storing it in various compartments such as vegetation, litter and soil organic matter. One of the islands which have a coastal forest with good enough condition is Kotok Besar Island, the cluster of Seribu Islands. This study aims to estimate the carbon stored in tree stands at coastal forest region of west side of Kotok Besar Island, Seribu Islands. Methods of calculating carbon stock was done by making the observation plots and distributed by random sampling method to calculate diameter at breast height (dbh) and identify the type of tree stands were found in the observation plots. dbh data was inserted into allometric equation to obtain the potential of stored carbon estimates. The results showed that the coastal forest of west side of the Kotok Besar Island have the number of plant species that dominate as many as four species, which consists of Thespesia populnea, Casuarina sp., Calophyllum sp., and Cocos nucifera. Biomass value is 853.94 tons / ha and potential of carbon stored is 426.97 tons/ha.
INDEX AUTHOR AND SUBJECT VOL. 11 NO. 1 APRIL 2018 INDEX INDEX
Al-Kauniyah: Jurnal Biologi Vol 11, No 1 (2018): Al-Kauniyah Jurnal Biologi
Publisher : Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islami

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (179.623 KB) | DOI: 10.15408/kauniyah.v11i1.10038

Abstract

PENGETAHUAN MAHASISWA TERHADAP KEANEKARAGAMAN TUMBUHAN DI LINGKUNGAN KAMPUS (STUDI KASUS PRODI PENDIDIKAN BIOLOGI UKI) Marina Silalahi
Al-Kauniyah: Jurnal Biologi Vol 9, No 1 (2016): Al-Kauniyah Jurnal Biologi
Publisher : Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islami

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (385.004 KB) | DOI: 10.15408/kauniyah.v9i1.3254

Abstract

Abstrak Penelitian bertujuan untuk mengetahui pengetahuan mahasiswa Program Studi (Prodi) Pendidikan Biologi FKIP UKI terhadap keanekaragaman tumbuhan di lingkungan kampus Universitas Kristen Indonesia Cawang. Penelitian dilakukan 2 tahap yaitu eksplorasi tanaman di lingkungan UKI dan survei terhadap 30 orang mahasiswa Prodi Pendidikan Biologi UKI tahun ajaran 2014/2015. Sebanyak 35 tanaman dibuat dalam bentuk 2-3 buah foto (habitus, bunga dan buah) untuk setiap spesies dan ditanyakan nama lokal, nama ilmiah dan famili masing-masing tumbuhan. Untuk melengkapi data mahasiswa, juga ditanyakan asal daerah dan etnisnya. Analisis data dilakukan secara deskriptif menggunakan statistika deskriptif. Mahasiswa Prodi Pendidikan Biologi mengetahui rata-rata nama lokal sebanyak 67%, nama ilmiah spesies sebanyak 24%, nama famili sebanyak 19%.Abstract Research was conducted to study the student knowledge on plant diversity in Biology Department, FKIP UKI in Universitas Kristen Indonesia Cawang.  The study was done in 2 stages, i.e plant exploration in UKI environment and survey on 30 students of Biology Department, UKI, academic year 2014-2015. 35 plant species were captured in 2-3 pieces of photo (habitus, flower, and fruit) for each species. Local names, latin names, and family names of each species were prepared. Origin and ethnic of each student was listed to fulfill student data. Data analysis was conducted by using descriptive statistics. Research found that students were aware of local names, species scientific names, and families names, respectively 67%, 24%, and 19%.
ISOLASI DAN KARAKTERISASI BAKTERI TANAH SAWAH DI DESA SUKAWALI DAN DESA BELIMBING, KABUPATEN TANGERANG Arief Pambudi; Susanti Susanti; Taufiq Wisnu Priambodo
Al-Kauniyah: Jurnal Biologi Vol 10, No 2 (2017): Al-Kauniyah Jurnal Biologi
Publisher : Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islami

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (745.781 KB) | DOI: 10.15408/kauniyah.v10i2.4907

Abstract

Abstrak Penggunaan pupuk kimia secara berlebih dapat menyebabkan kerusakan tanah dan menyebabkan ekosistem yang ada didalamnya terganggu. Plant growth-promoting rhizobacteria (PGPR) adalah bakteri yang hidup di daerah rizosfer tanaman yang dapat berperan sebagai biofertilizer, biostimulan dan bioprotektan. Penelitian ini bertujuan untuk memperoleh dan mengetahui karakteristik bakteri tanah yang berasal dari dua area persawahan, lokasi pertama di Desa Sukawali (TGR 1) dan lokasi kedua di Desa Belimbing (TGR 2), Kabupaten Tangerang. Penelitian dilakukan dengan mengambil sampel tanah, kemudian sampel dikultur dalam media agar nutrien dengan pengenceran bertingkat. Total bakteri dihitung dan isolat yang diperoleh diuji kemampuan dan karakternya dalam menambat nitrogen (BPN), melarutkan fosfat (BPF), menghasilkan indole acetic acid (IAA), menghasilkan Hidrogen Cyanide (HCN), aktivitas katalase, jenis Gram dan karakter motilitas. Total bakteri yang dapat tumbuh dari kedua lokasi sebanyak 2,4x106 CFU/g dan 1,8x106 CFU/g. Kedua lokasi diperoleh total 45 isolat dengan seluruhnya positif BPN, 42 isolat positif BPF, 24 isolat menghasilkan IAA, 27 isolat menghasilkan HCN, 43 isolat katalase positif, 39 isolat Gram positif, 6 isolat Gram negatif, serta 41 isolat motil. Berdasarkan uji yang dilakukan, terdapat 16 isolat yang berpotensi sebagai pupuk hayati.  Abstract Excessive use of chemical fertilizer may cause soil damage and disturb the ecosystem. Plant growth-promoting rhizobacteria (PGPR) is a consortium bacteria that live in plant rhizosphere which acts as biofertilizer, biostimulant, and bioprotectant. The objective of this research is to isolate and investigate the characteristics of soil bacteria originating from two rice fields in Sukawali Village (TGR 1) and Belimbing Village (TGR 2), Tangerang Regency. The research was conducted by collecting soil samples and then culturing the bacteria onto nutrient agar medium with serial dilution. The total bacteria were calculated and the isolates obtained were examined for their ability and characteristics on nitrogen-fixation, phosphate solubilization, IAA production, HCN production, catalase activity, Gram assay, and motility. The total plate count from both TGR 1 and TGR 2 were 2.4x106 CFU/g and 1.8x106 CFU/g, respectively. From these locations 45 isolates obtained were positive nitrogen-fixer, 42 isolates were phosphate solubilizer, 24 isolates were IAA producer, 27 isolates were HCN producer, 43 isolates were catalase positive, 39 isolates were Gram-positive, 6 isolates were Gram-negative, and 41 isolates were motile. On the whole results, it was concluded that there were 16 isolates that could potential as biofertilizer. 

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