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Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

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Published by Universitas Gadjah Mada

ISSN : 01261312 EISSN : 23563931 DOI : http://dx.doi.org/10.19106/JMedSci005303202101

Core Subject : Science,

Immunology & microbiology Neuroscience

Journal of the Medical Sciences (JMedSci) or Berkala Ilmu Kedokteran (BIK) is an international, open-access, and double-blind peer-reviewed journal, published by Faculty of Medicine, Public Health and Nursing, Universitas Gadjah Mada Yogyakarta Indonesia. JMedSci aiming to communicate high-quality articles in the areas of biomedical science from basic to clinical sciences.The journal welcomes papers from original articles, case reports, reviews, and book reviews. All papers published in JMedSci are freely available as downloadable pdf files. The journal began its publication on March 1973 and published quarterly (January, April, July, and October). JMedSci is abstracted and indexed in DOAJ, Crossref, Google Scholar, Sinta, Indonesia One Search. JMedSci is accredited by Directorate of General Higher Education, the Ministry of Research, Technology, and Higher Education, Indonesia

Arjuna Subject : Kedokteran - Kedokteran (Lain-Lain)

Articles 8 Documents

Search results for , issue "Vol 47, No 2 (2015)" : 8 Documents clear

Could CD8/38 predict virological response to antiretroviral therapy (ART) in human immunodeficiency virus (HIV) infected patients? Umi S. Intansari; Yanri W Subronto; Yunika Puspa Dewi; Adika Zhulhi Arjana; Mohammad Juffrie; Marsetyawan HNE Soesatyo; Budi Mulyono
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 47, No 2 (2015)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

ABSTRACT The success rate of antiretroviral therapy (ART) depends on the efficacy of the drug and the immune system’s ability to control virus replication. Viral load (VL) examination is a standard test for ART monitoring. Unfortunately, this test is still very limited, especially in Indonesia. CD38 expression has been studied as a predictor for disease progression and decreases once ART is initiated. The aim of this study was to evaluate the possible usage of declining CD8/38 T-cell percentages in predicting the VL. Forty-five subjects naïve to ART were evaluated in this observational analytic study with a prospective design. Data collected included the medical history, physical examination, WHO clinical staging, complete blood counts, CD4 cell count, and plasma VL. These data then reevaluated six months after ART initiation. A paired t-test, and correlation test were used to analyze the data. Significant differences were found in all of the laboratory parameters between pre and post ART. There was a weak significant positive correlation between declining CD8/38 T-cell percentages and declining VL after 6 months of ART (r=0.33; p=0.026), with r2 = 0.11. Therefore despite the linear relationship, CD38 has limited value for prediction of VL.

Basic principles and diagnostic of colposcopy Heru Pradjatmo
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 47, No 2 (2015)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

ABSTRACTThe colposcope is an optical system that offers illumination and magnifications between 10 and 16 times. Colposcopy is the diagnostic test to evaluate patients with an abnormal cervical cytological smear, abnormal VIA (visual inspection with acetic acid application) or VILI (visual inspection with Lugo’s Iodine application), abnormal appearing cervix and directing biopsies. The colposcopic examination of the cervix starts with “General Assessment” to immediately recognize the level of examination reliability. Examination should assessed for three variables: 1) adequate or inadequate, with the reason given; 2) squamocolumnar junction visibility; and 3) transformation zone type. Colposcopic features and patterns will correspond with underlying specific histological features. The greater the expertise and experience of the colposcopist, the greater the confidence in the assessment of the atypical transformation zone (TZ). For practical purposes, the most important aspect is always the recognition or exclusion of underlying actual invasive disease. The presence or absence of precancerous lesions can confirm with colposcopy.

The influence of proton pump inhibitors on the effect of clopidogrel on the risk of recurrent ischemic stroke Endang Mahati; Jarir Atthobari; . Ngatidjan
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 47, No 2 (2015)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

Patients surviving with a stroke have an increased risk for recurrent stroke. Clopidogrel iswidely used to prevent recurrent stroke. However, clopidogrel may cause gastrointestinalbleeding (GIB). Therefore, proton pump inhibitors (PPIs) is recommended to reduce theside effect of clopidogrel. Unfortunately, these both drugs are metabolized in the liverby the same enzyme i.e. cytochrome P4502C19 (CYP219) that may reduce the effectof clopidogrel. The aim of this study was to evaluate the influence of PPIs on the effectof clopidogrel in the prevention of recurrent stroke. It was a hospital-based case controlstudy conducted in Bethesda Private Hospital, Yogyakarta involving 392 patients withrecurrent stroke as cases and 784 patients with first-ever stroke as controls. The exposureof PPIs during clopidogrel therapy on both cases and controls groups were recordedfrom prescription records for at least six months before index date. The duration of PPIsexposure were categorized as current expoure (less than two months before the indexdate) and recent exposure (2-6 months before the index date). The result showed therewas no influence of PPIs exposure on the effect of clopidogrel on the risk of recurrentischemic stroke (OR: 1.00; 95% CI: 0.56-1.79). Moreover, the current use of PPIs andclopidogrel could decrease the risk of recurrent ischemic stroke (OR: 0.04; 95% CI:0.01-0.41). In conclusion, there is no association between PPI exposure and the risk ofrecurrent stroke in patients receiving clopidogrel.

The effect of a-terpineol on cell cycle, apoptosis and Bcl-2 family protein expression of breast cancer cell line MCF-7 Damiana Sapta Candrasari; Sofia Mubarika; Mae Sri Hartati Wahyuningsih
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 47, No 2 (2015)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

ABSTRACT The cytotoxic activity of a-terpineol on T47D and HeLa cancer cell lines have been reported. This study was conducted to evaluate the effect of a-terpineol on cell cycle, apoptosis and Bcl-2 as well as Bax expression on MCF-7 cell line. The cytotoxic activity of a-terpineol was determined using MTT cell assay. Cell cycle and apoptosis were analysed using flowcytometry, whereas Bcl-2 and Bax expression were evaluated using immunohistochemistry. The results showed that a-terpineol had cytotoxic effect on the MCF-7 cell lines with an IC50 value of 33.0 ± 5.4 μg/mL. a-Terpineol induced cell accumulation in Sub-G1 lead to apoptosis of the MCF-7 cell. Moreover, a-terpineol inhibited Bcl-2 and induced Bax expressions. In conclusion, a-terpineol has potential anticancer activity against MCF-7 cancer cell line trough through cells cycle inhibition and apoptosis stimulation.

Physical comparison between Rampasasa Pygmy and Yogyakarta children of Indonesia Neni Trilusiana Rahmawati; Janatin Hastuti; Rusyad Adi Suriyanto
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 47, No 2 (2015)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

ABSTRACTIn growth studies, somatotyping allows one to characterize changes in physique during growth in order to monitor growth patterns and to better understand variations in adult physique. Information on the physique of children with short stature is limited In Indonesia the study of somatotype for Pygmy children had never been done. The aims of this study were to compare the physiques of Rampasasa Pygmy and Yogyakarta children and to evaluate factors that might lead to variability in physiques. The sample consisted of 61 Rampasasa Pygmy (32 boys and 29 girls) and 319 Javanese children in Yogyakarta (173 boys and 146 girls) aged 8–13 years. Height, weight, biepicondylar breadths of the humerus and femur, calf and upper arm circumferences, and skinfolds (at triceps, subscapula, calf, and supraspine) were measured on each subject. We used somatotyped by the Heath-Carter method. The results showed that the Pygmy children were shorter, lighter, and less endomorphic than the Yogyakarta children. Our findings suggest that the observed differences between Rampasasa Pygmy and Yogyakarta children could be related mainly to environment background in the two areas.

The effects of the ethanolic extract of mahogany seeds (Swietenia macrophylla King) on the renal function of streptozotocin-induced diabetic rats Lukman La Basy; Sri Lestari SR; Sri Kadarsih
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 47, No 2 (2015)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

Diabetes-associated oxidative stress causes glomerular hypertrophy, decrease ofglomerular filtration rate and inhibits cell proliferation that lead to the decrease of renalfunction as indiated by the increase of serum creatinine level and the presence ofprotein in urine. Mahogany seed (Swietenia macrophylla King) has been proven to haveantidiabetic activity. This study was conducted to evaluate the effect of the ethanolicextract of mahogany seeds on the renal function of streptozotocin-induced diabetic rats.Six normal rats as control (Group I) and 24 diabetic rats were used in this study. Thediabetic rats were randomized allocated into four groups with six rats in each group.Group II was considered as diabetic rats control and received aquadest. Group III-V wereconsidered as extract administered diabetic group and received ethanolic extract of S.macrophylla seed for 21 days at a dose of 50, 100 and 200 mg/kg BW, respectively.Serum malondialdehyde (MDA), serum creatinine, and urine protein levels were monitored,before and after the ethanolic extract of S. macrophylla seed administration. Serum MDA,serum creatinine and urine protein levels of all rats after STZ induction (Group II-V) weresignificantly higher than without STZ induction (p < 0.05). A significant decrease in theserum MDA and serum creatinine as well as urine protein levels were observed after thetreatment with ethanolic extract of S. macrophylla seed compared to before treatment(p < 0.05). In conclusion, the ethanolic extract of S. macrophylla seed is able to correctrenal dysfunction of streptozotocin-induced diabetic rats.

The effect of proton pump inhibitors and clopidogrel combination to the risk of recurrent stroke Ngatidjan, Endang Mahati Jarir Atthobari
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 47, No 2 (2015)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (71.551 KB)

Recurrent stroke is very common among stroke survivors. Clopidogrel is one of the drugs used in the prevention of recurrent stroke. Because clopidogrel may cause gastrointestinal bleeding, the use of proton pump inhibitors may elicite the side effect, but unfortunately this combination may interfere the antiplatelete activity of clopidogrel since both are metabolized by the same enzyme, liver CYP2C19. The aim of this study was to know the effect of proton pump inhibitors on the efficacy of clopidogrel in the prevention of recurrent stroke in Bethesda Hospital, Yogyakarta. This study was a case control study. The sample size of treatment case was 392 patients and control case was 784 patients. The case group was patients with recurrent stroke and the control group was the patients who come to outpatient clinics of Department of Neurology. In this study, matching was made for treatment and control groups on sex, age and their index date. The exposure of the combination was investigated in the last 6 months and categorized as current use (<2 month before index date) and recent use (2-6month before index date). Multivariate analysis showed that there was no association between clopidogrel and proton pump inhibitors to the risk of recurrent stroke OR 1.05 (95% CI 0.56-1.785). Omeprazole was the most used, although there was no significantly increased risk OR 0.46 (95% CI 0.56-3.70). The effect of proton pump inhibitors to the efficacy of clopidogrel has not been known yet in this study, because the amount of total sample that used the combination of this drug was too small.Keywords: clopidogrel - proton pump inhibitors - recurrent stroke - interaction

THE EFFECTS OF THE ETHANOL EXTRACT OF MAHONI SEEDS (Swietenia macrophylla King) ON THE DIABETIC RATS INDUCTED WITH STREPTOZOTOCIN Sri Kadarsih, Lukman La Basy Sri Lestari S R
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 47, No 2 (2015)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

Show Abstract | Download Original | Original Source | Check in Google Scholar

background: Hyperglycemia causes oxidative stress and lead to diabetes complications. MDA is the result of peroxyde lipids due to the increasing ROS and causes glomerular hypertrophy resulted in a decrease marked with the increasing filtrate rate with the increasing creatinine serum and the presence of protein in the urine. Swietenia macrophilla King has as antidiabetic activity of which its parts to be used are often seeds that contain saponins.Objective: The study was conducted to determine the effects on renal protection by observing the serum creatinine parameters, MDA, and urinary protein.Methods: The subjects were 30 Wistar strain white rats aged 6-8 weeks weighing 180-200 grams. The subjects were conditioned with 60 mg/kgBW streptozotocin induced diabetes. Subjects were grouped into the control group, the diabetes, the ethanol extract of mahogany seeds (S. macrophylla King) 50 mg/kgBW group, 100 mg/kgBW and 200mg/kgBW groups. The administering of ethanol extract of mahogany seeds (S. macrophylla King) was conducted for ± 21 days and serum creatinine inspection parameters, MDA and urinary protein before and after the extract mahogany.Results: The study showed that within the group of ethanol extract of mahogany seeds (S. macrophylla King) 50 mg/kgBW, 100 mg/kgBW, 200 mg/kgBW there are decreasing levels of blood glucose, serum creatinine, urine protein MDA and significant with p <0.05 and ethanol extract of mahogany seeds (S. macrophylla King) 200mg/kgBW give effect best.Conclusion: Administering ethanol extract of mahogany seeds (S. macrophylla King) to Wistar strain rats can prevent complications towards impaired renal function by observing the decline of serum creatinine, MDA and urinary protein. Keywords : hyperglycemia, Swietenia macrophylla King, serum creatinine, MDA, urinary Protein.

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Home Page

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Nurhadiyahya

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+6289672800034

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