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INDONESIA
SAINSTIS
Published by Universitas Islam Negeri Maulana Malik Ibrahim Malang
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Core Subject : Education,
Education
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Articles 20 Documents
 1   2 
PENGUJIAN POTENSI BAKTERI ENDOFIT TERHADAP PERTUMBUHAN POPULASI NEMATODA SISTA KUNING (Globodera rostochiensis) PADA TANAMAN KENTANG (Solanum tuberosum L.) Ulfah Utami; Lilik Hariani; Retno Setyaningrum
SAINSTIS SAINSTIS (Vol 1, No 2
Publisher : Lembaga Penelitian dan Pengembangan Universitas Islam Negeri Maulana Malik Ibrahim Malang.

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (916.08 KB) | DOI: 10.18860/sains.v0i0.2301

Abstract

Yellow Nematoda sista (Globodera rostochiensis) is one of interfering plant organism in potatoes plants that bothers farmers in Indonesia. Many controls done by farmers during this time uses chemist pesticide. The using of chemist pesticide  continuously  can  threaten  human  environment  and  healthy.  The existence of endofit bacteria might became one of nematode controlling agent which is hospitable, because endofit bacteria can produce toxic compound in nematode. The aim of this research is to find out the filtrate potential of endofit bacteria to the growth of yellow nematode sista population (Globodera rostochiensis) in potatoes plants.  This research is done in Microbiological and Greenhouse Laboratory, Biology Department, Science and Technology Faculty, UIN Maulana Malik Ibrahim Malang and Pest and Plant Disease Laboratory, Agriculture Faculty, Universitas Brawijaya Malang.  This research is done in December  2011  until  April  2011  by  using  Rancangan  Acak  Lengkap  (RAL) method. Bacterial filtrate is made in a way of making endofit bacteria grow in TSB  media  for  48  hours,  then  disentrifugasi  with  speed  13.000  rpm  for  15 minutes. Next, it is tested in yellow nematode sista in potatoes plants in the greenhouse. The result of this research shows that endofit bacteria filtrate can obstruct the growth of sista Globodera rostochiensis population. Three from those six isolates which have high ability in pressing sista Globodera rostochiensis population in 100 gram of soil are isolate AA (91%), DH (81%) and BE (81%). Isolate AA can press sista Globodera rostochiensis population in the high scale, which reach until 91%. All endofit bacteria isolate (Isolat AA, AH, BA, BE, DA and DH) can increase the growth of potatoes plants, they are the height of plant and the weight of potatoes plants root.
PENGGUNAAN DOKSISIKLIN HYCLATE SEBAGAI INHIBITOR MATRIKS METALLOPROTEINASE PADA TERAPI TAMBAHAN PERIODONTITIS Risma Aprinda Kristanti
SAINSTIS SAINSTIS (Vol 1, No 2
Publisher : Lembaga Penelitian dan Pengembangan Universitas Islam Negeri Maulana Malik Ibrahim Malang.

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (787.015 KB) | DOI: 10.18860/sains.v0i0.2302

Abstract

Terbit.com  Daily  has  reported  that  the  prevalence  of  periodontitis increases related with age person. Found that 35,7% of patients with periodontitis in the group 30-39 years old to 66,5% in the group 50-59 years old, increase 89,2% in the group 80-90 years old. Periodontitis that commonly found is a chronic periodontitis which is occurs in individuals older than 45 years, but can also be found in children. The main characteristic of periodontitis is the damage of the periodontal connective tissue, alveolar bone, and the movement of the junctional epithelium to the apical. Bacterial components may directly or indirectly cause tissue damage. Inflammatory    mediators    product    such    as    proteinases,    cytokines,    and prostaglandins are part of the host response that can also cause tissue damage. Matrix metalloproteinase (MMP) is a proteinase that can cause periodontal tissue damage by disrupting the extracellular matrix molecules in periodontal tissues. Since is known that MMP has a role in various pathological processes, therapy to inhibit activity MMP in pathological processes has developed. MMP inhibitors will be a useful adjunct therapy for the treatment of periodontal disease. Many products have been introduced as an MMP inhibitor, for example: tetracycline and various derivatives, such as doxycycline and minocycline which are capable of inhibiting the activity of several classes of MMPs. Doxycycline has the ability to inhibit the increase in collagenase activity that is pathological in subgingival and prevent periodontitis to become more severe.
HIDROLISIS GUGUS METOKSIL PEKTIN AMPAS TEBU UNTUK MENGHASILKAN BIOMETANOL Akyunul Jannah; Arief Suryadinata
SAINSTIS SAINSTIS (Vol 1, No 2
Publisher : Lembaga Penelitian dan Pengembangan Universitas Islam Negeri Maulana Malik Ibrahim Malang.

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (711.294 KB) | DOI: 10.18860/sains.v0i0.2303

Abstract

Methanol is a potential in two areas, namely as fuel cells and biodiesel. Among the many biomass, pectin is one of the best for the production of methanol. The purpose of this study was to determine the influence of pectin methyl esterase enzyme  concentration  (PME)  group  metoksil  rough  on  pectin  hydrolysis  of bagasse to produce the highest levels of biometanol. Methods This study includes the preparation of the bagasse, further hydrolysis using crude extract of the enzyme pectin methyl esterase (PMEs) resulting from bacterial culture Bacillus subtilis at Borth Nutrient media (NB) with 1% pectin. Hydrolysis of Metoksil groups of poligalakturonat acid in the pectin bagasse to obtain the concentration of methanol to vary the enzyme crude extract PMEs. Concentration of crude enzyme extract PMEs used was 10%, 15%, 20% and 25% in 24-hour incubation with 2 repetitions performed. The resulting methanol levels were analyzed using GC (Gas Chromatography). The results of this study concluded that allowing the concentration used for hydrolysis of bagasse to produce methanol using crude enzyme from  Bacillus subtilis (PMEs) is 10% with a methanol content of 0.02%.
EVALUASI POTENSI HEPAR SEBAGAI SUMBER ENZIM PLATELET ACTIVATING FACTOR ACETYLHIDROLASE (PAF-AH) PADA PROSES ATEROGENESIS Retno Susilowati
SAINSTIS SAINSTIS (Vol 1, No 2
Publisher : Lembaga Penelitian dan Pengembangan Universitas Islam Negeri Maulana Malik Ibrahim Malang.

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (839.521 KB) | DOI: 10.18860/sains.v0i0.2304

Abstract

The atherogenesis is a fairly complicated process that deposit cholesterol in wall of arterial blood. Pathogenic atherosclerotic plaque formation requires very long time and it is often called as the silent killer. PAF-AH is a proatherogenic enzyme whose levels in serum rise rapidly since the beginning of the process atherogenesis. The sources of PAF-AH other than macrophages in the atherosklerostic plaque not been widely studied. The liver is the organ where the place of lipid metabolism and susceptible to oxidative stress. Oxidized Lipids serve as a stimulator of the synthesis of PAF-AH, so it needs to be explored its potential as a source of PAF-AH. This study aims to evaluate the liver as the source of PAF-AH on atherogenesis process that occurs in mice. This research was conducted based on post Test Control Design.This research is to observe PAF-AH levels in the lysates serum and liver. Histological observations of the presence of PAF-AH enzymes in liver tissue with the Immunohistochemistry are also observed in normocholesterol mice than hypercholesterolemic rats’ diet for 30 days. Data were analyzed with Anova test and correlation analysis. The results showed that hyperclolesterolemia rats had higher levels of PAF-AH in serum than normocholesterolemia mice (408.35 ± 142.23 218.93 ±106.07 ng /ml). Hypercholesterolemia mice had higher levels of PAF-AH in the liver lysate than normocholesterol mice (554.81 ± 143.33 275.60 ± 89.68 ng/ml). PAF-AH levels in serum correlate with levels in serum, PAF-AH liver lysate is more than the levels in serum. Immunohistochemistry show that enzyme PAF- AH found in liver tissue constituent cells.
KARAKTERISASI EKSTRAK TEH HITAM DAN TINTA CUMI-CUMI SEBAGAI FOTOSENSITISER PADA SEL SURYA BERBASIS PEWARNA TERSENSITISASI Rachmawati Ningsih; Hastuti Hastuti
SAINSTIS SAINSTIS (Vol 1, No 2
Publisher : Lembaga Penelitian dan Pengembangan Universitas Islam Negeri Maulana Malik Ibrahim Malang.

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (762.536 KB) | DOI: 10.18860/sains.v0i0.2305

Abstract

One of the alternative energy potential to be developed to overcome  energy crisis in the world is an organic solar cells. In this research has been conducted by making a series of Dyes Sensitized Solar Cells (DSSC) using an organic dye from crude extracts of black tea (Camellia sinensis) and ink cuttlefish (Sepia acuelata). Solar cell system consisting of crystalline TiO2, glass conductors, dyes, a   electrolyte   solution   I-/I3- and   carbon   electrodes.   TiO2 crystals   were characterized using X-ray diffraction. Crude extracts of black tea and cuttlefish ink absorption wavelength was analyzed using UV-Vis spectrophotometer. The series of solar cells are measured values of voltage and electric current. The results of X-ray diffraction showed that crystalline TiO2 including anatase crystalline phase and a crystal size is 2.6 nm. The results of UV-Vis spectra of crude extracts of black tea and cuttlefish ink shows the absorption wavelength range in a row of 232-240 nm and 203-207 nm. Large solar cell power supply voltage without soaking, in a room without light and under a halogen lamp is 0.014 Volt and 0.023 Volt. Large voltage solar cells soaked in crude extracts of black tea for 1 hour at room without light and under a halogen lamp is 0.364 Volt and 0.401 Volt. While that is soaked for 2 weeks is 0.113 Volts and 0.18 Volts.
ANALISIS PERMUKAAN ZEOLIT ALAM MALANG YANG MENGALAMI MODIFIKASI PORI DENGAN UJI SEM-EDS Anton Prasetyo; Rini Nafsiati; Susi Nurul Kholifah; Agie Botianovi
SAINSTIS SAINSTIS (Vol 1, No 2
Publisher : Lembaga Penelitian dan Pengembangan Universitas Islam Negeri Maulana Malik Ibrahim Malang.

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1047.273 KB) | DOI: 10.18860/sains.v0i0.2306

Abstract

Nature zeolit is pore material much benefitted in industry sector as adsorben, katalis and many other else. The existence of this material in Indonesia is very exuberate, one of them is Malang. The efforts to improve zeolit quality have already much done by many researchers. In this research, there has been done characterization and zeolit modification in Malang land become mesopori material by using surfaktan CTAB as template. To characterize pore can be done by SEM test and to know the element content can be done by EDS test. From this, we know that from SEM test got result that mophologi structure of zeolit grain generally contains lempengs arranged insmoothly and tend to be amorf shape. SEM test is incapable to identify pore of zeolit such as   preparasi, kalsinasi or modified zeolit in order unidentified took pore changing. The result of EDS test occur that the changing of element content of nature zeolit composer in dealuminasi process. So there is changing of ratio Si/Al in nature zeolit where it is 3.002   and   after   dealuminasi   1.8994.   Whereas   the   element   content   after modification is similar with element content in zeolit having dealuminasi.
KEANEKARAGAMAN FAUNA TANAH DI TAMAN NASIONAL BROMO TENGGER SEMERU SEBAGAI BIOINDIKATOR TANAH BERSULFUR TINGGI Dwi Suheriyanto
SAINSTIS SAINSTIS (Vol 1, No 2
Publisher : Lembaga Penelitian dan Pengembangan Universitas Islam Negeri Maulana Malik Ibrahim Malang.

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (445.966 KB) | DOI: 10.18860/sains.v0i0.2307

Abstract

The using of bioindikator lately become more important with the main function is to describe the existence of relevancy with biotic factor condition and abiotic area. Land fauna is enough as land indicator because it has sensitive respond to land and climate management practice, having good correlation to the land characteristic that benefits and the ecology function can describe causal chain that connects land management decision to the last productivity and plant and animal health. This research has purpose to identify kind of land fauna found in Bromo Tengger Semeru national park, analyse kinds of land fauna and analyse land fauna that can be used as land bioindikator with high sulphur standard. The   research   uses   exploration   method,   that   arrange   land   fauna observation Bromo Tengger Semeru national park and taking land sample to be analyzed in laboratory. Taking sample location in Bromo, Jemplang area (near Bromo mountain). In each location is being observed to land fauna with plot 1 X 1 m, as many as 10 plots. Land fauna found in each plots is noted about genre and amount. The sample of the land is taken, then brought to the laboratory and analyzed to find out the content of pH land, S land, organic material and C/N land. The result of this research shows that land fauna found in Bromo Tengger Semeru national park are 10 ordo, contained 29 family. Land fauna diversity in Bromo mountain is in lower area if it is compared with other area, with index 2.07 variety. Jemplang area has index variety 2.35 and Penanjakan area has index the highest fauna diversity, that is 2.73. Bromo mountain area has total sulphur  74,683  mg/1000g  land  and  it  is  the  area of  research within  highest sulphur. Land fauna used as land bioindicator within highest sulphur is ordo Brachyderinae   for   indicator   the   existence   fauna   and   Mesoveliidae   for bioindicator the inexistence of fauna.
PENGARUH PAPARAN SUHU TERHADAP KUALITAS SPERMATOZOA MENCIT JANTAN (MUS,MUSCULUS) STRAIN JEPANG Ermiza Ermiza
SAINSTIS SAINSTIS (Vol 1, No 2
Publisher : Lembaga Penelitian dan Pengembangan Universitas Islam Negeri Maulana Malik Ibrahim Malang.

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (645.585 KB) | DOI: 10.18860/sains.v0i0.2308

Abstract

Spermatogenesis is a process of spermatozoa conception in tubulus seminiferus, which is influenced by various different factors, including hormonal, inhibit epididimis deterent, radiation and temperature. Conducive temperature in the regulation of spermatogenesis is below normal body temperature, which is 37°C. Maximum activity of most human enzim works within about 37°C, since temperature above 37°C will create denaturalization of enzim. Enzim is needed for cell metabolism. Exposure in high temperature resulted from a physical and psychological  stress  activates  central  and  peripheral  response  of  endoktrin system. Activating endoktrin system of hipotalamus-Hipofise-Adrenal involves neurohormon CRH, CRH to GnRH and disturbs activity of adenohipofise in producing FSH, LH which in turn disturbs spermatogenesis. The purpose of this research is to analyze the impact of the exposure in the temperature of 40°C upon quantity, mortility, and morphology of spermatozoa male mencit within one cicle of spermatogenesis. The research uses the method of Post Test Only Control Group Design conducted in April to October 2010 in Pharmachology Laboratorium of Medical Faculty of Unand Padang. The samples were taken from 24 male mencit of 2-3 months, with an avarage weight of 25-35 grams, divided into 4 groups, i.e. controlled group and 3 treated groups. The treatment was based on the lenght of exposure in the temperature of 40°C. This exposure lasts about 15, 30 and 45 minutes everyday in 36 days. The result is analyzed using Kolmogorof Smirnof Test,  data  from  normal  distribution  analyzed  with  Anova  Test,  if  result  is signifikan continued with Post-Hoc-Test (Bonferroni). If data from distribution is not normal analyzed with Kruskal Wallis. The   research   on   some   spermatozoa   using   Anova   test   indicates unsignificant relationship (p0,05) between controlled and treated group. However,   research   on   the   percentage   of   mortilitas   and   morphology   of spermatozoa shows significant differences (p0,05). Avarage decrease of amount, percentage of motility and morphology of abnormal spermatozoa is comparable to the time of exposure in the temperature of 40°C witih 36 days. Based on this research, it is safe to conclude that the exposure in 40°C gives a significant  impact  on  the  declining  quality  of    spermatozoa.  It  is  urged  that detailed researches should be conducted on the impact of exposure in high temperature upon the structure of spermatozoa moleculs, such as DNA, mitokondria and the level of tertosteron.
ANALISIS KUALITATIF FENILALANIN SECARA CHROMATOGRAPHY KERTAS DAN CHROMATOGRAPHY LAPIS TIPIS (Studi Awal Pengembangan Metode Deteksi Penyakit Phenylketonuria) Begum Fauziyah
SAINSTIS SAINSTIS (Vol 1, No 2
Publisher : Lembaga Penelitian dan Pengembangan Universitas Islam Negeri Maulana Malik Ibrahim Malang.

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (916.371 KB) | DOI: 10.18860/sains.v0i0.2309

Abstract

The qualitative analysis of fenilalanin as paper chromatography and thin layer chromatography is developed as the beginning study of detection phenylketonuria  disease.  The  result  of  identification  qualitatively fenilalanin     solution     with     concentration     varia tion     using     paper chromatography media shows that the clearest spot is gotten by concentration minimal 50 ppm. The result of identification qualitatively fenilalanin  solution  with  variation  concentration  1,  3,  5,  10,  25  and  50 (ppm) using thin layer chromatography shows that from the smallest concentration  that  is  1  ppm,  has  already  give  the  clear  qualitative experiment result, that is purple. Fenilalanin analysis with thin layer chromatography gives more sensitive result than with paper chromatography. memberikan hasil yang lebih sensitif daripada dengan chromatography kertas.
POTENSI ANTIMALARIA EKTRAK DIKLOROMETAN DAUN BUNGA MATAHARI (Helianthus annuus L.) SECARA IN VIVO PADA HEWAN COBA Roihatul Muti’ah; Elok K.H; Ijro’atu Ijro’atu
SAINSTIS SAINSTIS (Vol 1, No 2
Publisher : Lembaga Penelitian dan Pengembangan Universitas Islam Negeri Maulana Malik Ibrahim Malang.

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (919.639 KB) | DOI: 10.18860/sains.v0i0.2310

Abstract

Antimalaria experiment on this research aimed to know the activity of extract sunflower leaf to the growth of parasit Plasmodium berghei   in mice balb/c. Mice is being infected by interaperitonial with 106 Plasmodium berghei and divided into 5 groups of treatment, they are (1) positive control, (2) artemisin control 0,004 mg/g BB, , (3) extract sunflower of dose 1 (0,05 mg/g BB), (4) extract sunflower of dose 2 (0,5 mg/g BB) and extract sunflower of dose 3 (5 mg/g BB). Those experiment of antimalaria activity used Peter method. The therapy is done when degree of parasitemia after infected reach 5-15% that was counted as day -0 and it is given during 5 days. Monitoring the degree of parasitemia is done in day -0, day -1, day -2, day -3, and day -5. The result shows that all dose of extract diklorometan sunflower can kill parasit plasmodium berghei and degree of parasitemia reached 0% in day -3 and day -4 after therapy. The statistic analysis shows that the first, second, and third doses on the positive control group have siginificant differences with p 0.05. on the artemism group, first, second, and third doses doesn’t show significant differences with p 0.05. This shows that extract diklorometan having high potential as antimalaria and the effect is the same with artemism.  

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