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Brigitta Laksmi Paramita
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Fakultas Teknobiologi, Universitas Atma Jaya Yogyakarta, Jalan Babarsari No. 44, Sleman, Yogyakarta 55281, Indonesia
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Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati
Published by Universitas Atma Jaya Yogyakarta
ISSN : 25273221     EISSN : 2527323X     DOI : doi.org/10.24002/biota
Core Subject : Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology
Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati merupakan jurnal ilmiah yang memuat hasil-hasil penelitian, kajian-kajian pustaka dan berita-berita terbaru tentang ilmu dan teknologi kehayatian (biologi, bioteknologi dan bidang ilmu yang terkait). Biota terbit pertama kali bulan Juli 1995 dengan ISSN 0853-8670. Biota terbit tiga nomor dalam satu tahun (Februari, Juni, dan Oktober).
Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Pertanian dan Biologi (Lain-Lain)
Articles 22 Documents
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Search results for , issue "Vol 10, No 3 (2005): October 2005" : 22 Documents clear
Indeks Glisemik Kacang Buncis (Phaseolus vulgaris) dan Kacang Panjang (Vigna sesquipedalis) serta Uji Efek Hipoglisemiknya pada Tikus Sprague Dawley Marsono, Y.; Triwitono, Priyanto; Kanoni, Sri
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 10, No 3 (2005): October 2005
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (202.813 KB) | DOI: 10.24002/biota.v10i3.2879

Abstract

Glycemic index (GI) of foods has been suggested as an additional parameter in selecting foods for the diabetic patients in nutritional management. GI was measured as the area under the postprandial blood glucose curve for a food expressed as a percentage of the area after the consumption of reference food (bread). In this research two legumes were investigated including snap bean (Phaseolus vulgaris L.) and yardlong bean (Vigna sesquipedalis). The objectives of this research were (i) to determine the IG of the beans and (ii) to investigate the effects of these legumes on serum glucose concentration of alloxan-induced diabetic rats. Ten health and normal volunteers (not diabetic) were provided. The volunteers took an overnight fasting, blood were drawn in the morning and analyzed for serum glucose. Then they were given the test beans containing total carbohydrates equivalent to 25-g glucose to be consumed. Blood samples were drawn for glucose measurement every 30 minutes until 120 min after meal. Serum glucose was determined enzymatically and the glucose responses were drawn graphically to calculate the GI of the beans. These beans were evaluated for their hypoglycemic properties in alloxan-induced diabetic rats. They were fed modified AIN 93 diets contain 20% of energy derived from snap bean or yardlong bean for 28 days. Serum glucose concentrations were measured weekly. It was found that with white bread as the food standard the GI of the snap bean and yard long bean were 43 and 86, respectively. In 28 days of diet intervention, snap bean decreased the serum glucose level by 73% (from 212mg/dL to 56mg/dL). Similar effect were found for the yardlong bean. This bean can reduced serum glucose level about 73% (from 224mg/dL top 61mg/dL), but both were similar to control diet that decreased the serum glucose level about 72% .
Penghambatan Produksi Aflatoksin dari Aspergillus flavus oleh Kapang dan Khamir yang Diisolasi dari Ragi Tempe Purwijantiningsih, Ekawati; Dewanti-Hariyadi, Ratih; Nurwitri, C. C.; Istiana, Istiana (alm.)
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 10, No 3 (2005): October 2005
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (192.716 KB) | DOI: 10.24002/biota.v10i3.2874

Abstract

This research was carried out to study the inhibition of aflatoxin production of Aspergillus flavus by moulds and yeasts isolated from ragi tempe. The result showed that moulds found in the ragi tempe consisted of Rhizopus oligosporus, R. oryzae, R. cohnii, Mucor rouxii, Rhizomucor pusillus, Aspergillus niger and miselia sterilia. Yeasts found in the samples were Candida sp1, Candida sp2 and Trichosporon sp. Twelve mould, and yeast isolates were chosen to determine their ability on inhibition of aflatoxin production. Aspergillus flavus alone or in association with each of these isolates were inoculated in Potato Dextrose Broth and analysed for aflatoxin production at day 10. All combinations yielded less aflatoxin than the single culture of A. flavus. All isolates tested could inhibit aflatoxin production. The highest inhibition was shown by Candida sp1 (99,96%).
Klorofil Floresen dari Spesies C3 dan C4 dalam Responnya terhadap Cekaman Kekeringan Hamim, Hamim
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 10, No 3 (2005): October 2005
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (343.214 KB) | DOI: 10.24002/biota.v10i3.2876

Abstract

Klorofil floresen fotosintesis dari dua spesies C3 (gandum dan kale) dan dua spesies C4 (Echinochloa cursgallii dan Amaranthus caudatus) dianalisis dalam responnya terhadap cekaman kekeringan di rumah kaca. Tumbuhan ditanam dalam pot berdiameter 15 cm selama sebulan kemudian diberi perlakuan kekeringan dengan penundaan penyiraman hingga tumbuhan layu. Kuantum efisiensi maksimum dari sistem cahaya II fotosintesis (Fv/Fm), quenching foto kimia (qP) dan non-fotokimia (qN) dianalisis untuk mengetahui keadaan fotosintesis tumbuhan selama cekaman kekeringan. Walaupun tidak ada pola yang jelas dalam hal status air dari spesies C3 dan C4, cekaman kekeringan yang diberikan menyebabkan penurunan kadar air medium (MWC), potensial air (WP) dan potensial osmotik (OP) semua spesies.  Gandum mamiliki nilai WP dan OP yang paling rendah sementara E. crusgallii memiliki nilai yang paling tinggi akibat cekaman kekeringan. Kekeringan menyebabkan penurunan laju fotosintesis pada semua spesies yang ditandai dengan penurunan qP, namun hanya kale dan A. caudatus yang mengalami peningkatan qN akibat cekaman kekeringan. Tetap stabilnya qP dari E. crusgallii pada awal cekaman mungkin berkaitan dengan mekanisme C4 yang dimiliki oleh spesies ini.  Penurunan Fv/Fm pada E. crusgallii pada periode akhir cekaman menunjukkan bahwa spesies ini mengalami fotoinhibisi disebabkan cekaman kekeringan.
Eksplorasi Genom, terkuaknya misteri manusia (Kajian Buku) Mursyanti, Exsyupransia
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 10, No 3 (2005): October 2005
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (157.342 KB) | DOI: 10.24002/biota.v10i3.2882

Abstract

Eksplorasi genom manusia yang terwadahi dalam Human Genom Project (HGP) telah berhasil mengidentifikasi keseluruhan genom (DNA) manusia dalam waktu 13 tahun (1990-2003), dua tahun lebih cepat dari yang ditargetkan (15 tahun). Karya yang spektakuler ini memberikan banyak informasi tambahan mengenai genom manusia. Genom manusia yang dahulu diperkirakan berukuran tiga milyar basa, ternyata tersusun atas 2,3 milyar nukleotida yang terdiri dari ±30.000 gen, 50% gen tersebut sudah diketahui fungsinya. Kromosom no.1 mengandung gen paling banyak (2.968 gen) sedangkan kromosom Y mengandung gen paling sedikit (231 gen). Selain itu, diinformasikan pula bahwa pembeda manusia yang satu dengan yang lain terletak pada tiga juta lokasi single nucleotide polymorphisms (SNPs).
Studi Perilaku Teritorial Burung Cangak Abu (Ardea cinerea Linn.) di Kebun Raya dan Kebun Binatang Gembiraloka Yogyakarta Nugroho, Wahyu; Djuwantoko, Djuwantoko; Jati, Wibowo N.
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 10, No 3 (2005): October 2005
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (226.209 KB) | DOI: 10.24002/biota.v10i3.2877

Abstract

This study aim to see on ecological aspect of Cangak Abu specifically on its behavior in defending their terittory. This research was done from July to August 2004. Data collecting was done using territory mapping methods with three observation area (1st-3rd). One pair of Cangak Abu observed at each area. Cangak Abu showed some behavior such as vocalization, sound, visual display, visual display-sound, visual display-sound-shortflight, patroling flight and fighting. The territories on frist to third observation areas were OA : 9 m2; OA : 6 m2 and OA : 9 m2 respectively.
Keanekaragaman Burung di Sungai Mamberamo, Papua (Short Communication) Pattiselanno, Freddy
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 10, No 3 (2005): October 2005
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (176.101 KB) | DOI: 10.24002/biota.v10i3.2881

Abstract

TheMamberamo-Rouffaer-IdenburghRiverwatershed, one of the wetland sites inWest Papuais the largest tropical marshlands in northern Papua, covers 7,711,602 hectares and includes in the North Papuan Lowlands Endemic Bird Area (EBA). The Irian Jaya Biodiversity Conservation Priority-Setting Workshop (Conservation International, 1999) highlighted that theMamberamoBasinis an area with high priority for conservation. It is also acknowledged little biological information has been collected therefore it is urgent to carry out biological and ecological study in this particular sites.
Pengaruh Perbedaan Jarak Tanam terhadap Pertumbuhan Tiga Varietas Kedelai (Baluran, Bromo, dan Galunggung) Djukri, Djukri
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 10, No 3 (2005): October 2005
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (175.066 KB) | DOI: 10.24002/biota.v10i3.2878

Abstract

The objective of the research was to investigate the effect of sunlight radiation distribution in canopy on soybean plants biomass at different planting distances. This research was done experimentally using the Block Design. The independent variables were the soybean varieties and planting distance. The varieties used were Baluran, Bromo, and Galunggung and the planting distance used was 15x15 cm2 and 25x25 cm2 as blocks with 10 replications. The dependent variables were sunlight intensity and the plants biomass. The result of the research indicated that after 40 planting days, the sunlight intensity in the canopy, at soybean varieties factors was significant (p<0.05), and the planting distance was significant too. The plant biomass indicated that varieties factors had a significant effect (p<0.05), but the planting distance factors were not significant (p>0.05).
Metode Antagonistik Langsung untuk Skrining Bakteri Laut Penghasil Senyawa Anti-methicillin-resistant Staphylococcus aureus (MRSA) Isnansetyo, Alim; Kamei, Yuto
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 10, No 3 (2005): October 2005
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (217.974 KB) | DOI: 10.24002/biota.v10i3.2873

Abstract

Metode yang berbasis antagonistik langsung antara bakteri laut dan Staphylococcus aureus resistan metisilin (Methicillin-resistant Staphylococcus aureus, MRSA) berhasil digunakan untuk skrining bakteri laut penghasil senyawa anti-MRSA. Metode ini menggunakan medium agar lapis ganda untuk mendukung pertumbuhan bakteri laut dan MRSA. Beberapa bakteri laut penghasil senyawa anti-MRSA berhasil diisolasi dengan metode ini. Suatu bakteri laut, strain O-BC30 merupakan bakteri yang paling kuat menghambat pertumbuhan MRSA. Isolat-isolat bakteri yang didapat menunjukkan aktivitas yang stabil terhadap MRSA dan aktivitas ini juga konsisten dengan aktivitas ekstrak yang diperoleh dari sel bakteri yang dikultur pada medium agar. Metode ini sangat berguna dan efisien untuk skrining bukan hanya bakteri penghasil senyawa anti-MRSA tetapi juga bakteri penghasil antibiotik.
Analisis kandungan artemisinin pada kultur tunas Artemisia annua L. dengan lima karakter morfologi yang berbeda Ermayanti, Tri Muji; Al Hafiizh, Erwin; Aryanti, Aryanti; Sutedja, Lenny
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 10, No 3 (2005): October 2005
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (231.493 KB) | DOI: 10.24002/biota.v10i3.2875

Abstract

Artemisia annua L. is a medicinal plant that has an antimalarial drug againts Plasmodium falciparum strains which are resistant to traditional drugs such as quinine and chloroquine. This plant contains artemisinin as major compounds found on its leaves. Aim of the research was to analyze the artemisinin content of shoot culture of A. annua which has five different in leaf morphologies (Morphology type A, B, C, D and E). Previous investigation showed that shoot regenerated from callus has five different morphologies. This somaclonal variation was permanent after continuously subcultured onto MS medium with no addition of plant growth regulators. Artemisinin was detected using HPLC and it was comfirmed by LC-MS. The results showed that shoot culture type B contained the highest artemisinin content (0.171%) amongst other type of morphology. Shoot type D showed the lowest level of artemisinin (0.006%) which was not significantly different with type E (0.008%). Addition of BAP at concentration of 1 and 2 mg/l increased artemisinin level of the shoot culturs.
Komposisi Vegetasi Mangrove di Muara Sungai Siganoi Sorong Selatan - Papua Rahawarin, Yohanes Y.
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 10, No 3 (2005): October 2005
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (236.035 KB) | DOI: 10.24002/biota.v10i3.2872

Abstract

The research was aimed to figure out the structure and composition of mangrove species in the delta of Siganoi River, Inanwatan District, South Sorong Regency, Papua. Belt transect was used and the data was analyzed using formula according to Clintron and Novelly. Research showed that ten species (seven genus) from five families were found in this area. At tree and sapling stages, Sonneratia alba performed the highest density with 324,1 trees/ha and 765,5 sapling/ha respectively, while at seedling stage Aegiceras corniculatumc with 69 seedling/ha. The highest frequency was found on trees and sapling of Sonneratia alba 0,966 and 0,431 respectevely. At the seedling stage, the highest frequency was found on species Aegiceras corniculatum (3970).

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