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Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati

biota
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Published by Universitas Atma Jaya Yogyakarta

ISSN : 25273221 EISSN : 2527323X DOI : doi.org/10.24002/biota

Core Subject : Science, Agriculture,

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati merupakan jurnal ilmiah yang memuat hasil-hasil penelitian, kajian-kajian pustaka dan berita-berita terbaru tentang ilmu dan teknologi kehayatian (biologi, bioteknologi dan bidang ilmu yang terkait). Biota terbit pertama kali bulan Juli 1995 dengan ISSN 0853-8670. Biota terbit tiga nomor dalam satu tahun (Februari, Juni, dan Oktober).

Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Pertanian dan Biologi (Lain-Lain)

Articles 1,183 Documents

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Struktur Populasi Genetik Ayam Hutan Hijau Menggunakan Sekuen Hypervariable 1 D-Loop DNA Mitokondria Arifin Zein, M. Syamsul; Sulandari, Sri
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 13, No 3 (2008): October 2008
Publisher : Universitas Atma Jaya Yogyakarta

Thirty-three individuals from four populations of green junglefowl (Gallus varius) were collected throughout Indonesia: 14 from Central Java, 4 from Eastern Java, 3 from Sumbawa island and 12 from Flores island. The mitochondrial DNA D-loop the samples were analysed for sequence diversity. Twenty-five haplotypes with 28 polymorphic sites were identified within the first hypervariable-1 fragment (397 bp) of the D-loop. Fuâ€™s Fs value was -25.96 (all samples, P=0), indicating high genetic diversity and population expansion; a conclusion supported by a neighborâ€“joining analysis of the haplotypes. Though sample size per population varied between 3 and 14, the Fs values for the four populations, between -2.20 and -10.76, were all significant (P=0). Only one haplotype was shared between three populations (Central Java, Sumbawa island, and Flores island) by a total of seven individuals. Within populations, only three haplotypes were shared by three individuals. The remaining haplotypes were unique, indicating genetic differentiation between populations as confirmed by significant pairwise Fst values at P=0.05 in four out of the six population pairs (except two pairs of Central Java & Sumbawa island and Flores island & Sumbawa island). The diversity pattern observed at the mtDNA of the green junglefowl provides a baseline which may help to understand the recent population expansions of domesticated chickens from multiple centres of domestications. Our observations also suggest careful interpretation of the results of genetic characterization may be needed when applied to the management and conservation of species like the green junglefowl. As in other multiparous birds and mammals with a short reproductive interval, green junglefowl may have established distinct genetic entities in metapopulations across its geographic distribution.

Keanekaragaman Tumbuhan Bahan Kebugaran Dalam Naskah Lontar Rukmini Tatwa Masyarakat Bali Suryadarma, I.G.P.
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 15, No 2 (2010): June 2010
Publisher : Universitas Atma Jaya Yogyakarta

Rukmini Tatwa is a traditional health and beauty Balinese script. That script was written on the leaves of palm tree (Borrassus flabellifer). The basic concepts of healthy and beauty are generally considered the integrated result of physical, mental and emotional. Rukmini Tatwa is traditional beauty that refers the practice of using natural plants substances. The script documented more than one hundred plant use, composition and therapy. The plants are prepared in various ways. Parts of plant used are the leaves, flowers, root, stem, bark, fruit, seed and tuber. Most of the concoction is made simply by grinding and pounding. Its knowledge is equal to the traditional medicine. Rukmini Tatwa illustrated to maintain the family harmony from lack of fitness and beauty as well as to solve sexual problems.

Penentuan Daya Antibiosis Beberapa Rumput Laut Hijau (Chlorophyta) dari Pantai Drini, Yogyakarta: Sebuah Kajian Awal Sidharta, B. Rahardjo
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 8, No 2 (2003): June 2003
Publisher : Universitas Atma Jaya Yogyakarta

Penelitian ini bertujuan untuk menguak keberadaan dan nilai penting dari senyawa alamiah yang terkandung di dalam beberapa jenis rumput laut di Indonesia, khususnya yang tergolong sebagai anggota rumput laut hijau (Chlorophyta). Tujuan khusus penelitian ini adalah untuk mencari kemampuan antibiosis dari rumput laut hijau yang ditemukan di pantai Drini. Empat jenis rumput laut hijau yang diketemukan memperlihatkan kemampuan antibiosis yang bervariasi dibandingkan dengan perlakuan kontrol. Salah satu jenis, bahkan, menunjukkan daya hambat yang lebih kuat terhadap pertumbuhan mikroorganisme uji, E. coli, daripada larutan penisilin murni (10 mg).

Aktivitas Harian Bekantan (Nasalis larvatus Wurmb) di Hutan Karet Kabupaten Tabalong, Kalimantan Selatan Soendjoto, Mochamad Arief; Alikodra, Hadi Sukadi; Bismark, Muhammad; Setijanto, Heru
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 11, No 2 (2006): June 2006
Publisher : Universitas Atma Jaya Yogyakarta

The daily activities of the proboscis monkey (Nasalis larvatus Wurmb) living inrubber forests are poorly understood. The objectives of the research were to studyits daily movement pattern and to determine the time proportion of daily behaviors.The movement of the monkeys was followed by researchers on foot. The behaviorswere observed by scan technique in 15 second intervals for 30 minutes. In rubberforests, lowlands (baruh) were places where the monkey began and ended dailymovements and also conducted most activities. Most inter-water movements of themonkey (about 70%) were conducted twice a day. Statistically, movement distanceand rainfall were negatively and significantly correlated (Ò‘s = -0.58; p < 0.05; n = 14).The monkey spent more time for feeding in the morning and afternoon, and restingat midday. Most behaviors of feeding, resting, playing, and grooming (84.01%) wereconducted at a tree height of less than 15 m. The monkeys moved farther during thedry season to get water and did activities in accordance with height strata to getvarious foods, avoided disturbances, and got comfortable and safe places.

Isolasi Bakteri Pengakumulasi Poliposfat Sudiana, I Made
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 9, No 2 (2004): June 2004
Publisher : Universitas Atma Jaya Yogyakarta

Polyphosphate accumulating bacteria playÂ important key role on biological phosphorous removal. Activated sludge originated from wastewater treatment plant for drinking water production acclimated with anaerobic-aerobic phase in sequential batch reactor continuously succeed to establish microbial community that are able to adsorb glucose and acetate under anaerobic condition.Â Acinetobacter calcoaceticus S1 was an isolated bacterium from community performing organic substrate absorption. S1 is able to adsorb glucose and acetate under anaerobic condition. During substrate absorption an increase of orthophosphate in bulk solution indicating phosphate released out of cell. Reduction of glycogen and polyhydroxybutirate (PHB) took place under anaerobic phase. S1 is a community member of anaerobic-aerobic system for removal of phosphorous in influent wastewater.

Karakterisasi Enzim Komersial Siklodekstrin Glukanotransferase Naiola, Elidar; Widhyastuti, Nunuk
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 13, No 2 (2008): June 2008
Publisher : Universitas Atma Jaya Yogyakarta

The objective of this study is to investigate the characteristic of commercial enzyme Cyclodextrin Glucanotrasferase (CGTase) from Bacillus macerans. The CGTase was purified by dialysis, gel fitration and ion exchange chromatography. Study on Characterization of the enzyme showed that the hydrolytic activity of CGTase was 480 U/mg, the optimum tempetature and pH for enzyme reaction were 450C to 550C and pH 5.0 to 8.0, respectively. The CGTase was relatively stable after heating at 550C for 10 minutes, and maintained its activity at the pH 5.0 to 9.0. The enzyme activity was inhibited by the presence of 1 mM metal ions and cause CGTase lost approximately 40% of its activity. Among the metal ions it was found that Cu2+ was the strongest inhibitor, with presence of 1mM Cu2+ the residual activity of CGTase was 24.4%. Results of purification showed that Specific activities of the enzyme during purification were 269 U/mg (crude enzyme); 955 U/mg (dialysis); 481 U/mg (gel fitrations); and 544 U/mg (ion exchange chromatography).

Penelitian Tentang Biologi Reproduksi pada Brill (Colistium guntheri Hutton, 1926) di Perairan Otago Selatan, New Zealand Setyono, Dwi Eny
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 10, No 2 (2005): June 2005
Publisher : Universitas Atma Jaya Yogyakarta

Perkembangan gonad, musim pemijahan dan fekunditas ikan sebelah â€œbrillâ€ (Colistium guntheri Hutton, 1926) di perairan Otago Selatan, New Zealand telah diamati selama satu tahun. Sampel ikan ditangkap setiap bulan menggunakan â€œbottom otter trawlâ€. Indeks gonad, tingkat kematangan gonad dan distribusi frekuensi ukuran telur digunakan untuk menentukan perkembangan gonad dan musim pemijahan. Hasil penelitian ini menunjukkan bahwa perbandingan populasi ikan â€œbrillâ€ jantan dan betina tidak berbeda nyata dari 1 : 1. Pada ukuran panjang tubuh yang sama, ikan betina mempunyai berat badan yang lebih besar dari pada ikan jantan. Hasil analisa histologi menunjukkan bahwa C. guntheri termasuk kelompok ikan yang mempunyai perkembangan telur secara sinkroni. Berdasarkan perubahan nilai indeks gonad dan perkembangan ovari, jenis ikan ini diketahui mengalami pematangan gonad yang sangat cepat pada akhir musim gugur (Juni), yaitu ditandai dengan peningkatan nilai indeks gonad secara drastis dan ovari didominasi oleh telur pada tingkat kematangan akhir (final maturation stage). Musim pemijahan dimulai pada akhir musim dingin (Agustus) sampai musim panas (Januari), ditandai dengan penurunan nilai indeks gonad secara nyata dan ovari didominasi oleh telur yang telah masak (mature), hidrasi (hydrated) dan paska ovulasi (postovulatory). Fekunditas (batch fecundity) ditentukan dengan menghitung semua telur yang telah masak, mencakup sekitar 34% dari total telur di dalam ovari. Pada pengamatan ini diketahui bahwa fekunditas (Y) proporsional dengan berat gonad dalam gram (Wg) dengan persamaan regresi Y = 103 [(4.34 + 20.06 (Wg)], R2 = 0.95, dan fekunditas relatif per gram berat gonad adalah 18.760+1.150 telur.

Daya Dukung dan Laju Pertumbuhan Microcystis Hasil Isolasi dari Waduk Sutami pada Berbagai Variasi Konsentrasi Nitrat dan Fosfat dalam Medium Selektif B-12 Retnaningdyah, Catur; Suharjono, Suharjono; Soegianto, Agoes; Irawan, Bambang
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 15, No 3 (2010): October 2010
Publisher : Universitas Atma Jaya Yogyakarta

The main objective of this research was to calculate the carrying capacity and growth rate ofisolated Microcystis result in Sutami reservoir on a variety of nitrate and phosphateconcentrations in the B-12 selective medium. Research was conducted in the laboratory withpure experiments using completely randomized factorial design with factors of nitrateconcentration variation (8, 16, 32, and 64 ppm) and phosphate (0.2, 0.4, 0.8 and 1.6 ppm) in B-12medium. Repetition of the study was conducted three times at the same time. Microcystispopulation abundance which was counted every day until the stationary phase (day +30) wasused to calculate the rate of growth (Î²) and maximum abundance of Microcystis can besupported by each medium treatment (Î³). The results showed that the growth rate of Microcystiswas not significantly influenced by levels of phosphate in the medium but significantly positivelycorrelated with increasing nitrate concentration in the medium. Carrying capacity or themaximum abundance (Î³) of Microcystis was influenced by the combination of nitrate andphosphate in the B12 medium. Concentration of phosphate 0.4 ppm in medium combined withnitrate 8âˆ’64 ppm could support the highest abundance of Microcystis.

Pengaruh Tepung Kedelai Kaya Isoflavon terhadap Testosteron Serum, Jumlah Sel Leydig dan Jumlah Sel Spermatogenik pada Tubuli Seminiferi Testis Tikus (Rattus norvegicus) Astuti, Sussi; Sutyarso, Sutyarso
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 15, No 1 (2010): February 2010
Publisher : Universitas Atma Jaya Yogyakarta

The objective of this experiment was to evaluate the effects of isoflavone-riched soybean flour with different levels of isoflavone on testosterone level, total Leydig cells, and total spermatogenic cells in the seminiferous tubules of male rats. Diet was given as isonitrogen and isocaloric with 10% of dietary protein from casein. Twenty five male of Sprague Dawley weaning rats (21 days old) were divided into five groups and treated with isoflavone-riched soybean flour by oral administration with different levels (dosages). The treatment was conducted for 2 months. The treatment of isoflavone-riched soybean flour with higher dosage of isoflavone increased the testosterone levels in the serum and the total Leydig cells in the seminiferous tubules of male rats. The optimum dosage of isoflavone was 1.5 mg/day resulted in the highest total spermatogenic cells of rat testes. The treatment isoflavone-riched soybean flour with 1.5 mg isoflavone/day on male rats resulted in the testosteron level of 2.96Â±0.45 ng/ml, total Leydig cells 70.22Â±9.34; while the number of spermatogonia, spermatocytes, early spermatids, late spermatids, and total spermatogenic cells were 48.44Â±4.82, 60.00Â±3.43, 221.56Â± 16.12, 164.33Â±17.94, and 494.33Â±32.94, respectively.

Model Pertumbuhan Populasi untuk Pengendalian Populasi Akasia Berduri (Acacia nilotica (L.) Willd. Ex Del.) di Taman Nasional Baluran Supriyadi, Supriyadi
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 12, No 3 (2007): October 2007
Publisher : Universitas Atma Jaya Yogyakarta

The savannas in Baluran National Park have been severely invaded by Acacia nilotica. The invasion reduced grazing areas and created wildlife watching problems for tourists. Therefore, population control management should be developed. The aim of the study was to construct a population growth model in relation to the control of the population in the park. The model was an age structured one which consisted of seed class, age class <1 year, age class 1-<2 years, age class 2-<3 years, age class 3-<4 years, and age class ï‚³4 years. It was assumed that a temporary seed bank exists; there is no seed dormancy; seeds are produced by age class ï‚³4 years; and the number of seedlings is determined by available space. The population size was expressed as the number of individuals per hectare. The population control scenario included effects of complete elimination and partial elimination in the first year. Each of those was combined with seed harvesting. The total population growth pattern generated by the model was similar to the logistic one but with a bit oscillation before a stable population size was reached. More important parameters in the model were germination rate, seedling survival rate, number of seeds per individuals, maximum population size, and survival rate of age class ï‚³4. The simulation results showed that control measures of the population were effective when seed harvesting was carried out. A periodic partial elimination combined with seed harvesting might be useful. Seed harvesting should be applied every year to retard the growth and to prevent the spread of A. nilotica populations in the savannas.

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Brigitta Laksmi Paramita

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journal.biota@gmail.com

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Fakultas Teknobiologi, Universitas Atma Jaya Yogyakarta, Jalan Babarsari No. 44, Sleman, Yogyakarta 55281, Indonesia

Location
Kota yogyakarta,

Daerah istimewa yogyakarta

INDONESIA

Abstract

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Abstract

Abstract

Abstract

Abstract

Abstract

Home Page

OAI Link

Editorial Team

Contact

Reviewer

Google Scholar

Contact Name Brigitta Laksmi Paramita

Contact Email brigitta.laksmi@uajy.ac.id

Phone +6282329549978

Journal Mail Official journal.biota@gmail.com

Editorial Address Fakultas Teknobiologi, Universitas Atma Jaya Yogyakarta, Jalan Babarsari No. 44, Sleman, Yogyakarta 55281, Indonesia

Location Kota yogyakarta, Daerah istimewa yogyakarta INDONESIA

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Contact Name
Brigitta Laksmi Paramita

Contact Email
brigitta.laksmi@uajy.ac.id

Phone
+6282329549978

Journal Mail Official
journal.biota@gmail.com

Editorial Address
Fakultas Teknobiologi, Universitas Atma Jaya Yogyakarta, Jalan Babarsari No. 44, Sleman, Yogyakarta 55281, Indonesia

Location
Kota yogyakarta,

Daerah istimewa yogyakarta

INDONESIA