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Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati

biota
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Published by Universitas Atma Jaya Yogyakarta

ISSN : 25273221 EISSN : 2527323X DOI : doi.org/10.24002/biota

Core Subject : Science, Agriculture,

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati merupakan jurnal ilmiah yang memuat hasil-hasil penelitian, kajian-kajian pustaka dan berita-berita terbaru tentang ilmu dan teknologi kehayatian (biologi, bioteknologi dan bidang ilmu yang terkait). Biota terbit pertama kali bulan Juli 1995 dengan ISSN 0853-8670. Biota terbit tiga nomor dalam satu tahun (Februari, Juni, dan Oktober).

Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Pertanian dan Biologi (Lain-Lain)

Articles 1,183 Documents

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Profil Cemaran Bakteri Coliform pada Minuman Susu Segar yang Dijual Pedagang Kaki Lima di Daerah Istimewa Yogyakarta Amarantini, Charis; Budiarso, Tri Yahya; Suryanto, Regina
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 10, No 1 (2005): February 2005
Publisher : Universitas Atma Jaya Yogyakarta

Coliform contamination emerges public health case particularly by pathogenic E.coli O157 which is characterized by hemorrhagic colitis with diarrhea. In order to study the profile of coliform bacteria contamination in fresh milk that was sold by the sidewalk trader in Yogyakarta province, in this research coliform detection was done by cultivate fresh milk samples on the medium of Chromocult Coliform Agar (CCA) and enrichment cultures on the medium of Vancomycin-Trypticase Soy Broth (mVTSB). Dark-blue colonies isolated on CCA plates were then evaluated for the ability to ferment D-sorbitol on EOH medium in order to suspect pathogenic E.coli O157 colonies. The results show that coliform contamination was 1.103 - 1.107 CFU/ml, although it was not detected on all of the samples. Profile of coliform contamination in fresh milk samples consisted of Citrobacter, Enterobacter, and Klebsiella (1,0.103 Â - 1,2.107 CFU/ml), Shigella, Salmonella, and Yersinia (1,0.103 - 2,1.106 CFU/ml), and E.coli (1,4.104 - 2,8.104 CFU/ml). Dark-blue isolates that were suspected as pathogenic E.coli do not ferment D-sorbitol on EOH medium. Based on this result, it was concluded that it was not associated with E.coli O157.

Khelatisasi Ion Aluminium oleh Asam Organik Eksudat Akar Brachiaria Hafif, B.; Sabiham, S.; Iswandi, A.; Sutandi, A.; Suyamto, Suyamto
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 15, No 3 (2010): October 2010
Publisher : Universitas Atma Jaya Yogyakarta

Aluminum toxicity is one of the major factors inhibiting plant growth in acid soils. Brachiaria grass adapt to high Al concentration. This experiment was conducted to study exudation of low molecular weight organic acids (LMWOA) activated by Al, from Brachiaria roots and its potential in chelating Al. Three Brachiaria species, i.e. B. decumbens, B. ruziziensis and B. brizantha, planted in sterile sand culture and were treated with 5 Al concentrations (0, 100, 200, 300 and 400 Î¼M). After two-month experiment, three kinds of LMWOA, i.e, malic, citric, and oxalic acids, produced by the three Brachiaria-root exudates were measured in the sand culture. The production of malic acid was higher than that of citric and oxalic acid. Those organic acids were influenced by Al concentration; the higher Al concentration the higher organic acid content would be. The organic acids were also proved to form Al-organic compounds effectively of which B. decumbens and B. brizantha were more effective in chelating Al at relatively low Al (100 Î¼M) and at relatively high Al concentration (300 Î¼M and 400 Î¼M), respectively.

Isoflavon Kedelai Diperkaya dengan Zn sebagai Suplemen Antiarterosklerosis Wanita Premenopause Winarsi, Hery
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 12, No 2 (2007): June 2007
Publisher : Universitas Atma Jaya Yogyakarta

This research was conducted to observe the effect of soy isoflavone and Zn on totalcholesterol, LDL, TG and HDL levels of premenopausal women. Thirty-threepremenopausal women in Purwokerto were given supplement containing soy isoflavoneand Zn during 2 months. In the control group (SO), 11 women were given placebo; inthe group SI, 11 women were given the supplement containing soy isoflavone; and in thegroup SIZ, 11 women were given the supplement containing soy isoflavone and Zn.Blood samples were taken 3 times, i.e. baseline, 1 and 2 month after intervention. Oneml of blood was taken by venoject-heparin, intravenously. Blood plasma was used todetermine total cholesterol, LDL, HDL, and TG levels using spectrophotometer. Dataobtained were analysed by ANOVA. The total cholesterol, LDL, and TG plasma levelsdecreased, from 189,27 to 151,63 mg/dl (p=0,02); from 138,97 to 93,93 mg/dl (p=0,004);and from 208,81 to 121,09 mg/dl (p=0,012) respectively. On the contrary HDL levelincreased, from 48,38 to 67,27 mg/dl (p=1,72E-05) after 2 months of intervention. Soyisoflavone and Zn (SIZ) have potential as antiatherosclerosis supplement.

Kecocokan Jenis Inang dan Pemberian Pupuk Kandang terhadap Pertumbuhan Semai Cendana (Santalum album L. ) Kuswanto, Kuswanto
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 10, No 1 (2005): February 2005
Publisher : Universitas Atma Jaya Yogyakarta

The purpose of this research was find out the effects of three host plants and three levels of natural fertilizer on sandalwood seedling growth. This natural relationship between host plant and sandalwood seedling was conducted recognize the characteristic of seedling growth was studied. The parameters were height, diameter, and haustoria number.The research was conducted in factorial experiment arranged in CRD. The treatment consisted of two factors, namely effect of host plant and stable fertilizer. The first factor consisted four kinds of host plants were : a/ control; b/ Cabe rawit ; c/ Turi; and d/ Lamtoro, while the second factor were four levels of stable fertilizer : a/ control; b/ 200 g/pot; c/ 300 g/pot, and d/ 400g/pot.Three replicates was employed in the research.The result of the research indicated that most of different host plant and stable fertilizer treatments were significant effect on sandalwood seedling growth. After five months, the highest growth of Sandalwood seedling treatment with turi and stable fertilizer 400 g/pot was 44.92 cm , and 38.41 cm with host plant cabe rawit and stable fertilizer 300 g/pot. While the lowest was 12.80 cm produced by control (without host plant and fertilizer ). The diameter growth of sandalwood seedling treatment with turi and stable fertilizer 400 g/pot was 3,09 mm, and 3,30 mm with cabe rawit and stable fertilizer 400 g/pot. The houstoria number of sandalwood seedling treatment with turi was 75 and 71 with cabe rawit. In terms of determined the relationship between Sandalwood and its host plants were first turi , second cabe rawit, and lamtoro gung.

Struktur Anatomi Daun Artemisia cina Berg. Ex Poljakov Hasil Kultur Jaringan Ermayanti, Tri Muji; Juliarni, Juliarni; Andry, Yuli
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 9, No 3 (2004): October 2004
Publisher : Universitas Atma Jaya Yogyakarta

This study was conducted to investigate the anatomical structure of leaves of Artemisia cina Berg. ex Poljakov due to variances in leaf morphology during grown in vitro. Shaking of culture, position of stem nodes used as explants and concentration of BAP (Benzyl Amino Purin) added to the culture medium were the factors investigated in this study. There was variation in leaves morphology of culture, however this variation did not occur after aclimatization and plant grown in the greenhouse. BAP decreased the level of chlorophyll content of leaves. In general, anatomical characters of leaves were not influenced by shaking, nodes position of stem and level of BAP.

Sistematik Numerik Strain-Strain Anggota Genus Pseudomonas Pendegradasi Alkilbenzen Sulfonat Liniar Berdasarkan Sifat Fenotip dan Protein Fingerprinting Suharjono, Suharjono; Sembiring, Langkah; Subagja, Jusup; Ardyati, Tri; Lisdiana, Lisa
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 12, No 1 (2007): February 2007
Publisher : Universitas Atma Jaya Yogyakarta

Bacteria strains consisting of Pseudomonas sp. strain J and R isolated from river ecosystem polluted and Pseudomonas sp. strain A and B isolated from river ecosystem unpolluted by detergent were capable to degrade of LAS. The objective of this research was to determine similarity value by numerically of LAS-degrading Pseudomonas strains based on phenotype character and protein fingerprinting using three reference strains consist of Pseudomonas putida FNCC071, P. fluorescens FNCC070, and P. aeruginosa FNCC063. Phenotype characteristics examined are cellular and colony morphology, biochemical nature, capability to degrade polysaccharide, tolerance to various environmental factors and antibiotics, and ability to ferment sugar. Cellular protein fingerprinting was analyzed using SDSâ€“PAGE discontinuous. Strains classification was determined based on Simple Matching Method similarity index by UPGMA (Unweight Pair Group Method with Average) algorithm. Based on phenotype nature, all strains have similarity value 0.61; however, based on cellular protein fingerprinting, those strains have similarity value 0.52. All strains of LAS-degraded were including in the genus of Pseudomonas.

Karakterisasi, Pengaruh Sumber Nitrogen dan Karbon terhadap Produktivitas Enzim Lipase Rhizopus microsporus var oligosporus UICC 550 Mangunwardoyo, Wibowo; Lusini, Yuyun; Gandjar, Indrawati
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 14, No 2 (2009): June 2009
Publisher : Universitas Atma Jaya Yogyakarta

Effect of nitrogen and carbon source on the production of extracellular lipase by R.microsporus var. oligosporus UICC 550 was studied. The enzyme activity was alsocharacterized in terms of temperature, pH, stability at room temperature, and effectof divalent ion. The amount of 2.5% (w/v) of olive oil as carbon sources and 5% (w/v)peptone as nitrogen sources were the optimum for production of lipase enzyme.Partial purifications using ammonium sulfate followed by dialysis showed that at pH6.5 and temperature 35oC was the optimum condition, respectively. The stability(remaining up to 80% of the optimum enzyme) was recorded at pH 6.5 after 24 hoursincubation at room temperature. The optimum activity remained 40% of the after onehour incubation at 35oC. Divalent ions concentration at 1 mM, Zn2+, Cu2+, Mg2+ andFe2+ inhibited the lipolytic activity.

Deteksi Secara Imunohistokimia Oxygen-Free Radical Scavenger-Copper, Zinc-Superoxide Dismutase (Cu,Zn-SOD) pada Hati Tikus di Bawah Kondisi Stress Wresdiyati, Tutik
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 8, No 3 (2003): October 2003
Publisher : Universitas Atma Jaya Yogyakarta

Copper,zinc-superoxide dismutase (Cu,Zn-SOD) yang merupakan salah satu oxygen-free radical scavenger telah dideteksi secara imunohistokimia pada hati tikus di bawah kondisi stres.Â Sejumlah empat puluh lima ekor tikus jantan galur Wistar telah digunakan pada penelitian ini.Â Hewan percobaan tersebut dikelompokkan menjadi tiga kelompok, yaitu : (1) kelompok kontrol, (2) kelompok dengan perlakuan stres selama 3 hari, dan (3) kelompok dengan perlakuan stres selama 5 hari. Stres yang diberikan adalah puasa, dengan pemberian air minum secara ad libitum. Stres yang diberikan menimbulkan keadaan histopatologis, peradangan dan nekrosis pada jaringan hati kelompok perlakuan tersebut.Â Secara imunohistokimia menunjukkan adanya penurunan kandungan Cu,Zn-SOD pada jaringan hati kelompok perlakuan dibandingkan pada kelompok kontrol.Â Penurunan kandungan Cu,Zn-SOD tersebut lebih hebat pada kelompok perlakuan selama 5 hari dibandingkan dengan kelompok perlakuan selama 3 hari. Hasil penelitian ini menunjukkan bahwa kondisi stres kemungkinan dapat meningkatkan terbentuknya oxygen-free radical yang kemudian merusak jaringan hati dan menurunkan kandungan Cu,Zn-SOD.

Penggunaan Operkulum dalam Penentuan Umur pada Rhinoclavis sinensis Gmelin 1791 (Gastropoda: Cerithiidae) Zahida, Felicia; Subagja, Jusup
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 15, No 3 (2010): October 2010
Publisher : Universitas Atma Jaya Yogyakarta

Age determination of the sample specimen is very important for population dynamic research of R. sinensis (Gastropoda : Cerithiidae). Operculum is a â€œhardâ€ part stick on the dorsal portion of the foot of gastropods. Operculum appeared on the day of the snails are born and have their own specific shape, size, and materials composition. The aim of this research was to describe the use of operculum in age determination of R. sinensis. The method used in this research was: the operculums were soaked into saturated sodium hydroxide, washed and mounted them in Canada balsam, and observed using binocular microscope. Regression analysis was used to find the relationship between length of operculum to length of shell, and number of adventicious layers of operculum to length of shell. The research resulted that the length of operculum was comparable with the shell length, with the regression line of Y=0.113X +1.898 (R2=0.857). The growth of adventitiousâ€™ layers of the operculum coincided with the growth of the shell that in the second year, the growth of adventitious layers was two layers a year. At the age of three and four, the growth was one layer a year. During the fifth, sixth and seventh years, the growth was only a layer within three years. The regression line was Y=0.168X (R2=0.872).

Pengaruh Pemberian Ekstrak Kulit Batang Ailanthus altissima terhadap Perubahan Sel Hati dan Ginjal Mencit Praptiwi, Praptiwi; Kuncari, Emma Sri; Agungpriyono, Dewi Ratih; Chairunnisa, Chairunnisa
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 15, No 1 (2010): February 2010
Publisher : Universitas Atma Jaya Yogyakarta

The aim of this study was to determine the toxicopathological effect of Ailanthus altissima extract in liver and kidney of the mouse. The treatments in this study were: (1) negative control (carboxy methocellulose), extracts with concentration of (2) 1 mg/kg BW, (3) 10 mg/kg BW, (4) 100 mg/kg BW, and (5) 1000 mg/kg BW. Every treatment consisted of 5 mice. After receiving A.altissima extract for seven day consecutively, the mice were then euthanized with overdose of ether. The liver and kidney were taken and then fixed within 10% solution of Buffer Neutral Formalin (BNF). Histopathology slides of liver and kidney were processed and stained with Hematoxylline Eosin. The cell degeneration and cell death were observed with video photo microscope. The lesion of cell death and degeneration of hepatocytes and epithelium of kidney proximal tubule were counted. The data were analyzed statistically with ANOVA test continued with Duncan test. The result showed that cell necrosis of hepatocytes was not significantly different (P>0.05) compared to control treatment, while cell necrosis was increasing in the treatment of â‰¥ 10 mg/kg BW in tubuli of the kidney.

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Brigitta Laksmi Paramita

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journal.biota@gmail.com

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Fakultas Teknobiologi, Universitas Atma Jaya Yogyakarta, Jalan Babarsari No. 44, Sleman, Yogyakarta 55281, Indonesia

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Kota yogyakarta,

Daerah istimewa yogyakarta

INDONESIA

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Home Page

OAI Link

Editorial Team

Contact

Reviewer

Google Scholar

Contact Name Brigitta Laksmi Paramita

Contact Email brigitta.laksmi@uajy.ac.id

Phone +6282329549978

Journal Mail Official journal.biota@gmail.com

Editorial Address Fakultas Teknobiologi, Universitas Atma Jaya Yogyakarta, Jalan Babarsari No. 44, Sleman, Yogyakarta 55281, Indonesia

Location Kota yogyakarta, Daerah istimewa yogyakarta INDONESIA

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Contact Name
Brigitta Laksmi Paramita

Contact Email
brigitta.laksmi@uajy.ac.id

Phone
+6282329549978

Journal Mail Official
journal.biota@gmail.com

Editorial Address
Fakultas Teknobiologi, Universitas Atma Jaya Yogyakarta, Jalan Babarsari No. 44, Sleman, Yogyakarta 55281, Indonesia

Location
Kota yogyakarta,

Daerah istimewa yogyakarta

INDONESIA