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Brigitta Laksmi Paramita
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Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati
Published by Universitas Atma Jaya Yogyakarta
ISSN : 25273221     EISSN : 2527323X     DOI : doi.org/10.24002/biota
Core Subject : Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology
Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati merupakan jurnal ilmiah yang memuat hasil-hasil penelitian, kajian-kajian pustaka dan berita-berita terbaru tentang ilmu dan teknologi kehayatian (biologi, bioteknologi dan bidang ilmu yang terkait). Biota terbit pertama kali bulan Juli 1995 dengan ISSN 0853-8670. Biota terbit tiga nomor dalam satu tahun (Februari, Juni, dan Oktober).
Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Pertanian dan Biologi (Lain-Lain)
Articles 1,193 Documents
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Penapisan Aktivitas Selulase Isolat-isolat Khamir dari Moluska, Serasah, dan Tumbuhan di Taman Nasional Gunung Halimun, Jawa Barat Mangunwardoyo, Wibowo; Fitri, Reno; Oetari, Ariyanti
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 12, No 3 (2007): October 2007
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (297.223 KB) | DOI: 10.24002/biota.v12i3.2805

Abstract

A total of 236 yeast isolates from mollusc, litter, and plant samples from Gunung Halimun National Park were screened for cellulolytic activity based on Smith method by using 0,2% (w/v) cellulose-azure for 30 days. The results showed that 12 isolates (9 isolates from plants, 2 isolates from molluscs, dan 1 isolate from litter) have cellulolytic activity. These isolates were further screened based on Teather and Wood method for six days to determine their cellulases components by using specific substrates. Carboxymethyl cellulose (CMC) 0,1% (w/v) was used as a specific substrate to determine endoglucanase activity. Avicel 0,1% (w/v) was used as a specific substrate to determine exoglucanase activity. Cellobiose 0,1% (w/v) was used as a specific substrate to determine β-glucosidase activity. The results showed that 6 isolates from plant have β-glucosidase activity, and 1 isolate from plant have β-glucosidase and endoglucanase activities. Five isolates (2 isolates from plants, 2 isolates from molluscs, and 1 isolate from litter) showed no cellulase activity on specific substrates after six days incubation.
Peran Ovicidal Herbal Serbuk Biji Pepaya (Carica papaya L) Matang Terhadap Telur Cacing Ascaris suum Ardana, Ida
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 15, No 3 (2010): October 2010
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (313.292 KB) | DOI: 10.24002/biota.v15i3.2600

Abstract

An in vitro experiment to determine the effect of ground mature papaya seeds on characteristics of Ascaris suum eggs was conducted employing a Randomized Block Design. Four doses of the ground preparation – 0 mg (as control), 285 mg, 570 mg, and 855 mg- in 40 ml of physiological saline solution – were used to soak 100 eggs. Six replications were made. Worm eggs were obtained from the uteruses of ascariasis pigs slaughtered at the local abattoir. The eggs characteristics observed after application of the treatment were the embryo formation ability and the damage of egg cell layers. Statistical analysis was applied to the data collected using Analysis of Variance followed by Least Significance Test. The results showed that the ability of treated eggs to form embryos was significantly lower than that of control. Such a decrease in embryo formation ability could be related to the formation of granule (albumen coagulation) in the egg cell layers after the treatments. All treated eggs possessed such granules.
Karakterisasi α-Amilase Bacillus firmus KH.9.4 Alkalotoleran dari Limbah Cair Tapioka Rachmania, Nisa; Iswati, Ruma; Imas, Tedja
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 9, No 3 (2004): October 2004
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (200.421 KB) | DOI: 10.24002/biota.v9i3.2909

Abstract

Alkalotolerant bacteria identified as Bacillus firmus KH.9.4 was isolated from tapioca liquid waste from Kedung Halang, Bogor. The isolate showed optimum a-amylase activity after 15 hours of cultivation on liquid media. Optimum enzyme activity occurred at pH 6.0 and temperature 800C. Upon heat treatment at pH 6.0 and 800C the enzyme was still 100% active after 6 hours incubation without substrate. The enzyme activity was enhanced by supplement 10 mM MgSO4.7H2O. The presence of 1, 5, and 10 mM ethylenediaminetetraacetic acid demonstrated the inhibitory effect to this enzyme. Zymogram analysis revealed one translucent zone corresponding to soluble starch activities after 60 minutes of incubation at 400C in the 0.05 M phoshate buffer pH 7.0. Whereas SDS-PAGE analysis showed that there are 5 bands of proteins.
Analisis Filogenetik Burung Maleo (Macrocephalon maleo) Berdasarkan Sekuen Intron Satu Gen Rhodopsin (RDP1) Nukleus Budiarsa, I Made; Artama, I Wayan Tunas; Sembiring, Langkah; Situmorang, Jesmandt
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 15, No 2 (2010): June 2010
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (244.988 KB) | DOI: 10.24002/biota.v15i2.2693

Abstract

The phylogenetic relationships of the maleo (Macrocephalon maleo) were analyzed based on thefirst intron of rhodopsin nuclear gene sequence data obtained from 15 individuals, along withthose of 22 individuals taken from GenBank. The phylogenetic trees were reconstructed byNeighbor-Joining (NJ) method. Results indicated that 956 bp of RDP1 sequence, 414 (43.4%)sites were variable and 317 (33.2%) sites were phylogenetically-informative. The basecomposition for all species analyzed in this research were as follows: T 25.3%, C 26.3%, A18.5%, and G 29.9%. Analysis of RDP1 sequence produced trees that were remarkably wellresolved and had topologies at the marga level. The phylogenetic analysis showed that maleowas monophyly of Macrocephalon and closely related to Aepypodius, Talegalla, Leipoa andAlectura.
Menguak Rahasia Kehidupan (Kajian Buku) Lanni, Fransiska
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 8, No 3 (2003): October 2003
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (232.805 KB) | DOI: 10.24002/biota.v8i3.2860

Abstract

Masih ingat dengan James D. Watson, Francis Crick dan Maurice Wilkins, trio ilmuan yang dianugerahkan hadiah Nobel untuk bidang Fisiologi dan Kedokteran tahun 1962? Penemuan mereka tentang struktur double helix DNA yang spektakular tersebut telah membuat revolusi besar dalam  biologi molekular dan tidaklah berlebihan jika  kemudian dijuluki sebagai “the father of DNA”. Memperingati setengah abad (50 tahun) publikasi pertama tentang double helix di Nature (1953), telah diluncurkan buku  DNA:The Secret of Life.
Inventarisasi Jenis dan Distribusi Habitat Rotan pada Hutan Dataran Tinggi dan Dataran Rendah di Kalimantan Selatan Arifin, Yudi Firmanul
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 13, No 3 (2008): October 2008
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (179.347 KB) | DOI: 10.24002/biota.v13i3.2567

Abstract

Rattans are spiny climbing palms in the Old World tropics and subtropics. Some species are the source of cane for the cane furniture industry in Indonesia. The large number of rattan species and their wide geographical range is matched by great ecological diversity. The restriction of rattan species to different climatic zones and altitudinal range suggests that these species may have precise climatic requirements and also altitudes. The research was conducted on upland and lowland of natural forest in South Kalimantan. The data collection used survey with transect method. Volume of rattans in upland forest was relatively lower than lowland forest. In altitudinal range in South Kalimantan, rattans were found from sea-level up to 870 m, the highest altitude record being held by Calamus javensis, Daemonorops micracantha, Calamus pilocellus, and Calamus sp. (rotan tunggal). While the lowest altitude record was occupied by Calamus trachycoleus. Rattans in natural forest require light intensity, humidity and temperature were 18– 65%, 60 – 70%, 28 – 33oC, respectively.
Keanekaragaman Jenis Ekhinodermata Di Perairan Minahasa Utara, Sulawesi Utara Yusron, Eddy; Susetiono, Susetiono
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 15, No 2 (2010): June 2010
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (570.78 KB) | DOI: 10.24002/biota.v15i2.2710

Abstract

Observation on echinoderms diversity was carried out at coastal waters of North Minahasa bay, North Sulawesi, from June to August 2008. Sampling was done by using a transect quadrant of 1 m x 1 m. The results showed that in both locations, 28 species of echinoderms represented 7 species of Holothuroidea, 9 species of Echinoidea, 6 species of Asteroidea and 6 species of Ophiuroidea had been found in the North Minahasa Bay, North Sulawesi. The Echinoidea were relatively common in the sea grass zone. On the basis of population density, Echinoidea was the dominant group and had the highest density. This research was aimed to find out the species composition, community structure, zonation, and spatial distribution of echinoderm fauna.
Keanekaragaman Burung di Sungai Mamberamo, Papua (Short Communication) Pattiselanno, Freddy
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 10, No 3 (2005): October 2005
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (176.101 KB) | DOI: 10.24002/biota.v10i3.2881

Abstract

TheMamberamo-Rouffaer-IdenburghRiverwatershed, one of the wetland sites inWest Papuais the largest tropical marshlands in northern Papua, covers 7,711,602 hectares and includes in the North Papuan Lowlands Endemic Bird Area (EBA). The Irian Jaya Biodiversity Conservation Priority-Setting Workshop (Conservation International, 1999) highlighted that theMamberamoBasinis an area with high priority for conservation. It is also acknowledged little biological information has been collected therefore it is urgent to carry out biological and ecological study in this particular sites.
Notes of Honey Buzzard and Other Birds in Dumai and Rupat Island, Riau Province, Indonesia Sukmantoro, Wishnu; Cye, Lim Kim; Tiah, Lim Au; Iqbal, Mohammad; Ng, Francis
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 11, No 2 (2006): June 2006
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (156.132 KB) | DOI: 10.24002/biota.v11i2.2637

Abstract

Dumai are a town that located coastal area in the Nothern part of Pekan Baru, a capital city of Riau Province (1o 41’ 04.1” N, 101o 26’ 14,1” E). Dumai is margined directly by Rupat Island and Bengkalis Island in the North and Duri District in the South and East. On February 17, survey is conducted in Dumai harbour which vegetation are dominated rural plant and small scale mangrove. Only few records of bird are reported on 1980 such as storm stork Ciconia stormi (Holmes 1980).
Identifikasi Awal Bakteri pada Juwana Trochus niloticus Linn. dan Tridacna squamosa Linn. Asal Hatchery Pulau Barrang Lompo Makassar Litaay, Magdalena; Gobel, Risco B.; Abdullah, As’adi; Subair, Subair
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 12, No 3 (2007): October 2007
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (458.708 KB) | DOI: 10.24002/biota.v12i3.2800

Abstract

The research on early identification of bacterial from juveniles top shell (Trochus niloticus L.) and giant clam (Tridacna squamosa Linn.) was conducted at Unhas’s hatchery at Barrang Lompo island during August-October 2006. Bacteriology test was done at Microbiology Laboratory of Biology Department, Math and Science Faculty, Hasanuddin University, Makassar. The quantitative test was done using Most Probable Number (MPN) and Standard Plate Count (SPC) methods. While the qualitative test included bacteria colony observation, macroscopic, microscopic, and biochemical test. Macroscopic observation was done by assessing the form, elevation, color, ridge, and inner structure of bacterial colony. Microscopic observation was conducted by using Gram and spora stain. The result of MPN method shows the average total bacteria for juveniles top shell is 17.5 x 102 cell/ml and for giant clam is 6.65 x 102 cell/ml, while SPC results indicate the average total bacteria for juveniles top shell is 4.8 x 105 cell/ml and for giant clam is 3.0 x 105 cell/ml, respectively. The result of biochemical test identifies 5 genera of bacteria such as Micrococcus, Bacillus, Streptomyces, Escherichia and Enterobacter.

Page 31 of 120 | Total Record : 1193

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