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Brigitta Laksmi Paramita
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Fakultas Teknobiologi, Universitas Atma Jaya Yogyakarta, Jalan Babarsari No. 44, Sleman, Yogyakarta 55281, Indonesia
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Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati
Published by Universitas Atma Jaya Yogyakarta
ISSN : 25273221     EISSN : 2527323X     DOI : doi.org/10.24002/biota
Core Subject : Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology
Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati merupakan jurnal ilmiah yang memuat hasil-hasil penelitian, kajian-kajian pustaka dan berita-berita terbaru tentang ilmu dan teknologi kehayatian (biologi, bioteknologi dan bidang ilmu yang terkait). Biota terbit pertama kali bulan Juli 1995 dengan ISSN 0853-8670. Biota terbit tiga nomor dalam satu tahun (Februari, Juni, dan Oktober).
Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Pertanian dan Biologi (Lain-Lain)
Articles 1,193 Documents
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Struktur Komunitas Moluska Bentik di Perairan Sekitar PLTU Grati, Pasuruan, Jawa Timur Arbi, Ucu Yanu
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 15, No 3 (2010): October 2010
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (507.729 KB) | DOI: 10.24002/biota.v15i3.2595

Abstract

Observation on marine benthic molluscs around PLTU Grati waters in Pasuruan was conducted on May and December 2004. The aim of this study was to determine condition and community structure of benthic molluscs living in those areas. Samples were collected using Eckmann grab and sieved through 0.5 mm mesh-sized. The number of molluscs species is 28 species, consisting of 16 species class of gastropoda and 12 species class of pelecypoda. A diversity index (H) ranged 0.845 to 1.158, an evenness index (J) was 0.856 to 1 and a richness index (D) was 19.562 to 31.949. Littorina undulata (Littorinidae) was a dominant species and was relatively widely distributed. In general, this result showed that around PLTU Grati waters had a relatively low benthic molluscs diversity.
Bioinformatika: Sebuah Perhelatan Iptek Paling Spektakuler Abad Ini (Kajian Buku) Sidharta, Boy Rahardjo
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 9, No 2 (2004): June 2004
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (246.148 KB) | DOI: 10.24002/biota.v9i2.2902

Abstract

Kemajuan dan perkembangan di bidang Biologi Molekuler semakin menjadi jelas ketika banyak negara (setidaknya beberapa negara Eropa, terutama Inggris serta Amerika Serikat) terlibat dalam suatu proyek mahabesar yang bertajuk Human Genome Project (HGP). HGP sendiri merupakan salah satu tahap lanjutan dari penerapan Bioteknologi (BT) bagi kemaslahatan manusia. Hal ini menandai pula era BT memasuki milenium ketiga ini.
Struktur Anatomi Epidermis Daun Lima Kultivar Melon (Cucumis melo L.) Berdasarkan Resistensinya terhadap Jamur Tepung (Sphaerotheca fuliginea Poll) Maryani, Maryani; Prabawani, Ratri Lila; Daryono, Budi Setiadi
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 14, No 2 (2009): June 2009
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (344.435 KB) | DOI: 10.24002/biota.v14i2.2688

Abstract

Powdery mildews are plant diseases caused by fungi, including Sphaerotheca fuliginea Poll. They cause a decrease on leaves health. Plant resistance to Sphaerotheca fuliginea Poll. might have a relationship to leaf anatomical structure, especially to epidermis. Therefore, the aim of this research was to characterize epidermal leaf structure of resistant and susceptible cultivars. This study used two resistant cultivars (PI 371795 and PMAR), one susceptible cultivar (Action 434) and two other cultivars (Fresh 848 and Sakata 144) which are not known their characteristic to powdery mildews. The fifth leaves (counted from the cotyledon) were collected from melon plants which reached early generative development. Leaf epidermal slides were prepared semi permanently. The results showed that the morphological leaves of resistant cultivars have dendatus leaf edges, while the susceptible one has repandus leaf edges. Anatomically, the leaves of PI 371795 had upper epidermal cells with wavy edges, but not in the others. Both PI 371795 and PMAR 5 had irregular shape of lower epidermal cells with curved edges, while leaf epidermal cells from susceptible cultivar tended to have irregular polygonal upper and lower epidermal cells with straight edges and shorter non-glandular trichomes. PMAR 5 had long glandular trichomes. There was significant difference between cultivars in the average of stomata number, the average of stomata index and trichome number on the lower epidermal tissue, but not on the lower epidermal tissue. In the lower epidermis, PI 371 and PMAR 5 had lower average of stomata number and stomata index compared to Action 434. PMAR 5 had higher number of trichomes than susceptible cultivar (Action 434). Meanwhile, Fresh 848 cultivar tends to have similar characters to resistant ones in terms of leaf edges, lower epidermal shape, stomata number and index. Sakata 144 had similar characters to those the susceptible one in terms on leaf edges, lower epidermal cells shape, stomata number and index, but not in trichome number. It may be assumed that the first protection of melon against S. fuliginea Poll. is located on lower epidermal tissue.
Isolasi dan Karakterisasi Jamur Pendegradasi Katekin dari Seresah Pinus Nurnawati, Elisa; Sembiring, Langkah
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 8, No 3 (2003): October 2003
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (318.808 KB) | DOI: 10.24002/biota.v8i3.2855

Abstract

Isolation of catechin-degrading fungus from pine litter samples was done using minimal medium that containing catechin as sole carbon and energy source.  A total of 53 isolates were chosen to represent different colonial types of catechin degrading-fungus. The isolates were screened for their ability to degrade catechin in three stages. The first stage of screening was based on their ability to grow on solid medium containing 2 mM, and as a result, 28 isolates were selected.  The second stage of screening on the same medium but containing 4 mM of catechin resulting in 14 selected isolates. The third stage screening was based on their mean growth rate constant (k), instantaneous growth rate constant (m) and generation time (g) on minimal medium containing 4 mM catechin. The result showed that four isolates (D9, K2, K11, and S11) were the best catechin degradator. Further growth kinetic study  (k, m ,and g) of selected  isolates   indicated that  D9, K2, and S11 grew well on the medium containing 40 mM, but  K11 was inhibited by concentration of higher than 10 mM. Catechin biodegradation process was determined by following the decrease of catechin concentration on liquid medium. It was found that isolate K2 had higher ability to degrade catechin than the isolate K11. Finally, the four selected isolates from the third stage were characterized in terms of macroscopic, microscopic and phenotypic characters and identified. The result of the study showed that the isolates D9, K2 and S11 were identified as member of Aspergillus niger group. The isolate D9 was very similar to isolate S11, while the isolate K2 was found to be the most similar with Aspergillus niger van Tiegh. IFO 6341. The isolate K11 was assigned to be member of the genus Trichoderma.
Pengaruh Penggunaan Berbagai Jenis Ikan sebagai Inang terhadap Kelangsungan Hidup Glochidia Kijing Taiwan (Anodonta woodiana Lea) Hamidah, Afreni
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 11, No 3 (2006): October 2006
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (264.148 KB) | DOI: 10.24002/biota.v11i3.2547

Abstract

The research about the glochidia Anodonta woodiana Lea viability on host fishes has been conducted in the Laboratory of Zoology Biology Department Mathematic and Science Faculty Sriwijaya University from July – November 2004. The research applied completely randomized design with 5 treatments of host fish (Cyprinus carpio, Pristolepis fasciatus, Oreochromis niloticus, Trichogaster pectoralis and Clarias gariepinus) with 6 replications. The results of the research showed that the use of fishes host has influenced the glochidia viability. The highest glochidia viability was in Trichogaster pectoralis there was a great diffoence compared to other host fish. This result showed that Trichogaster pectoralis was the most effective host fish of Anodonta woodiana glochidia.
Kajian Molekular Tarsius sp. Pada Gen Penyandi Cytochrome Oxidase Sub-unit 2 Mitokondria Widayanti, Rini; Handayani, Niken Satuti; Budiarsa, I. Made
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 15, No 1 (2010): February 2010
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (467.782 KB) | DOI: 10.24002/biota.v15i1.2653

Abstract

Tarsius is an endemic species in Indonesia that is endangered. In-situ and ex-situ conservation of this species would yield better results if this genetic make up and diversity is determined. The objective of this ressearch was to study the specific genetic marker on COX2 gene of Tarsius sp. Sequencing of PCR product using primer COX2F and COX2R resulted in base sequence of 513 nts. Results of COX2 fragments sequencing were put on multiple alignment with other primates from Genbank with aid of software Clustal W, and were analyzed using MEGA program version 4.1. Eight different amino acid sites were found (amino acid no. 5, 6, 13, 14, 30, 35, 44 and 168). The genetic distance based on nucleotide COX2 calculated using Kimura 2-parameter model indicated that in the smallest genetic distance 0%, biggest 6.8% and average 2.3%. The phylogenetic tree using neighbor joining method based on the sequence of nucleotide and amino acid COX2 reveded differentiation among Tarsius from Lampung and Tarsius from Sulawesi, but could not be used to differentiate among T. dianae (from Central Sulawesi) and T. spectrum (from North Sulawesi).
Karakterisasi Protease Ekstraseluler Clostridium spp. T11-3 Natalia, Loli; Nathalia, Lily; Meryandini, Anja
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 11, No 1 (2006): February 2006
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (264.063 KB) | DOI: 10.24002/biota.v11i1.2822

Abstract

Protease is one of the leather commercial enzymes which is widely used such in food processing, medicine and leather industry. Clostridium sp T11-3 was isolated from Tiu Jeruk River in Nusa Tenggara Barat. Sequence analysis of 16S rRNA indicated that Clostridium spp T11-3 was closely related to C. bifermentans. This isolate produced maximum protease activity after 18 hours of cultivation in liquid media. Protease of Clostridium spp 11-3 displayed maximum activity at pH 5 and 60oC with casein as substrate. In the presence of 1 mM divalent ion Mg2+ the enzym activity increased to 141 %, while others ion divalent (Ca2+, Zn2+, Cu2+, Fe2+, and Co2+) inhibited protease activity.
Kualitas Keripik Bengkuang (Pachyrrhizus erosus (L.) Urb.) dengan Variasi Konsentrasi Ekstrak Bunga Rosela (Hibiscus sabdariffa Linn.) Setyasih, Martina; Pranata, F. Sinung; Purwijantiningsih, Ekawati
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 13, No 1 (2008): February 2008
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (123.353 KB) | DOI: 10.24002/biota.v13i1.2616

Abstract

Yam bean chips have crispy texture and sweet taste. In this research, rosella extract is added in the mold of yam bean chips as natural preservaties, fiber and sources anthocyanin. The aim of this research is to know the effect of various consentrations of rosella extract on chips qualities. Research design utilized was randomized complete factorial design and the data compiled was treated by Anova and followed with Duncan’s Multiple Range Test (DMRT) with degree of convidence 95%. Chips with 0,67% rosella extract added has longer shelf life than chips with out rosella extract added. This Chips contain 0,59% water, 2,6% protein, 38,84% fat, 3,69% ash, 54,16% carbohydrate, 1,17 mg/100g ascorbic acid, 1,4 mg/l anthocyanin, 1,17% acid total, 86,74%fiber crude, 53,47 level of lightness and total microorganism 30 CFU/G.
Aktivitas Antibakteri Ekstrak Karang Lunak Sarcophyton sp. yang Difragmentasi dan Tidak Difragmentasi dari Perairan Pulau Pramuka, Kepulauan Seribu, Jakarta Kawaroe, Mujizat; Soedarma, Dedi; Effendi, Hefni; Nurhayati, Tati; Hardiningtyas, Safrina Dyah
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 15, No 3 (2010): October 2010
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (350.539 KB) | DOI: 10.24002/biota.v15i3.2588

Abstract

Fragmented and non-fragmented soft corals showed antibacterial activities. Soft corals were gradually extracted using methanol, ethyl acetate, and hexane. Crude extract of the samples was tested its antibacterial activity, Minimun Inhibitory Concentration, toxicity (Brine Shrimp Lethality Test method), and phytochemicals. Overall, the antibacterial activity of crude extract of non-fragmented soft coral Sarcophyton sp. was higher than the crude extract of fragmented soft coral Sarcophyton sp. Crude ethyl acetate extract showed higher antibacterial activities. The ethyl acetate crude extract of non-fragmented soft coral Sarcophyton sp. is able to inhibit all tested bacteria is E. coli, S. aureus, P. aeruginosa and B.cereus, while the ethyl acetate crude extract of fragmented Sarcophyton sp. is unable to inhibit bacteria P. aeruginosa. Minimum inhibitory concentration extracts of non-fragmented Sarcophyton sp. in range 240−480 μg/disc. The 24-h LC50 extracts of fragmented and non-fragmented Sarcophyton sp. for Artemia salina were 149.50 ppm and 45.15 ppm, respectively. Bioactive compounds of fragmented and non-fragmented Sarcophyton sp. extract are steroid, flavonoid and alkaloid.
Pola Pertumbuhan dan Produksi -Amilase Bacillus amyloliquefaciens pada Substrat Pati Jagung dengan Variasi pH Awal Media dan Waktu Inkubasi Wahyuningsih, Sisilia Sri; Mursyanti, Exsyupransia; Atmodjo, P. Kianto
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 9, No 2 (2004): June 2004
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (319.385 KB) | DOI: 10.24002/biota.v9i2.2895

Abstract

The aims of this study  were to identify the growth curve of  B. amyloliquefaciens on  corn-starch and non corn starch addition media,  number of cells  and production of a-amylase on variety initial pH during the stationary phase. The growth curve of B. amyloliquefaciens was made using the water optical density on both  medium which has inoculated by microbes. The experimental design for the a-amylase production was factorial completely randomized design (6 x 3 x 3). There were two factors included in this study i.e. initial  pH of the media ( 5,  5.5,  6,  6.5,  7 and 7.5) and incubation times (16, 18 and 20 hours). The results showed that B. amyloliquefaciens growth curve on medium with corn starch was slower than on medium without corn starch. Production of  a-amylase and number of cells were having similar patterns in all treatments, i.e. increased until optimum pH and incubation time were reached. The number of cells and a-amylase production were optimal at pH 6.5 for 18 hours incubation whereas the number of cells  (about 2.8542 x 108 cells/ml)  and a-amylase production (1.4467 units/ml) were optimal at pH 6.5 for 18 hours  incubation.

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