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Brigitta Laksmi Paramita
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Fakultas Teknobiologi, Universitas Atma Jaya Yogyakarta, Jalan Babarsari No. 44, Sleman, Yogyakarta 55281, Indonesia
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Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati
Published by Universitas Atma Jaya Yogyakarta
ISSN : 25273221     EISSN : 2527323X     DOI : doi.org/10.24002/biota
Core Subject : Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology
Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati merupakan jurnal ilmiah yang memuat hasil-hasil penelitian, kajian-kajian pustaka dan berita-berita terbaru tentang ilmu dan teknologi kehayatian (biologi, bioteknologi dan bidang ilmu yang terkait). Biota terbit pertama kali bulan Juli 1995 dengan ISSN 0853-8670. Biota terbit tiga nomor dalam satu tahun (Februari, Juni, dan Oktober).
Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Pertanian dan Biologi (Lain-Lain)
Articles 1,193 Documents
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Pertumbuhan Mikroalga Chlamydomonas dalam Medium Beneck dan Air Tanah yang Mengandung Insektisida Berbahan Aktif Klorpirifos Kurniasih, M.; Prihantini, N. B.; Nurtiyani, E.
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 8, No 1 (2003): February 2003
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (276.111 KB) | DOI: 10.24002/biota.v8i1.2827

Abstract

The research of insecticide effect to the growth of genus Chlamydomonas had been done.  Research was experimental study with block random design to 9 concentrations of chlorpiriphos containing insecticide in Beneck and soil water media i.e. 0 (control), 0.05, 0.10, 0.15, 0.20, 0.25, 0.30, 0.35, and 0.40%.  Observations were done for 35 days.  Friedman test showed that there were some effects of media and insecticide concentrations on cell numbers of Chlamydomonas (cell/ml) in culture (p>0.05).  Dunnets test showed that mean of cell numbers of Chlamydomonas (cell/ml) differ (on p>0.05) and very differ (on p>0.01) on every concentrations in Beneck and soil water media.  Insecticide could reduced the cell size of Chlamydomonas, yet increased the number of cells on 0.05--0.30% concentrations in Beneck medium, and 0.05--0.20% concentrations in soil water medium.  On peak culture, insecticide with 0.10% concentration produced the highest cell numbers i.e. 21,179,167 cell/ml in Beneck medium, and 4,087,500 cell/ml in soil water medium.
Produksi Fitoaleksin pada Tusam (Pinus merkusii Jungh. et de Vriese) sebagai Respon Infeksi Fungi Mikorisa Widyaningsih, S.; Widyastuti, S. M.; Sumardi, Sumardi
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 11, No 2 (2006): June 2006
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (328.029 KB) | DOI: 10.24002/biota.v11i2.2621

Abstract

The experiment aimed to detect (1) mycorrhizal infection induced phytoalexins production of the root of tusam and (2) its activity in suppressing a dumping off fungi, Fusarium sp and Rhizoctonia solani. Production of phytoalexins was detected from the extracts of mycorrhizal root of old trees and 4, 6 and 8 week-old seedlings in alcohol using a UV-spectrophotometer. The antifungal activity of phytoalexins was studied by inoculating the pathogenic fungi into mycorrhizal seedlings of tusam. The results showed that the spectra of phytoalexins of extracted mycorrhizal and non-mycorrhizal roots ranged from 203.2 to 204.6 nm. Higher antifungal concentrations of antifungal compound was obtained from the root extracts of old trees and 4 week-old seedlings, indicated by the higher spectrophotometric absorbance (0.315 and 0.324 g-1 root ml-1 ethanol), compared to that extracted from non-mycorrhizal root of the same plant origin. Mycorrhizal roots significantly suppressed the development of Fusarium sp. and Rhizoctonia solani.
Pengaruh Interaksi Genotipe dengan Lingkungan terhadap Kandungan Fe Biji Padi Suwarto, Suwarto; Nasrullah, Nasrullah; Taryono, Taryono; Sulistyaningsih, Endang
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 15, No 2 (2010): June 2010
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (464.551 KB) | DOI: 10.24002/biota.v15i2.2727

Abstract

To explore the effect of genotype, environment and genotype x environment interaction on Fe content in rice grain, Fe concentrations of 10 genotypes were analyzed across eight paddy field environments using the GGE-biplot method. Experiments were conducted from 2007 to 2008 using a randomized completely block design with three replications at each environment. Results indicated that environment (E), genotype (G) and genotype x environment interaction (GE) significantly gave effect on Fe rice concentration. Environment explained 74.43% of total (G + E + GE) variation, whereas G and GE captured 5.60% and 19.67%, respectively. The first two principal components (PC1 and PC2) were used to create a two-dimensional GGE-biplot and explained 70.40% and 15.36% of GGE sum of squares. Genotype Barumun was desirable in terms of its highest Fe rice concentration ability and stability. In Cilongok, wet season was the best representative of the overall environments and the most powerful to discriminate genotypes.
Produktivitas Primer Rawa Jombor, Klaten, Jawa Tengah Anggraeni, Belinda Dian; Jati, Wibowo Nugroho; Zahida, Felicia
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 8, No 2 (2003): June 2003
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (260.344 KB) | DOI: 10.24002/biota.v8i2.2886

Abstract

Primary productivity of Jombor Swamp, Klaten has been assessed in order  to understand its water quality. The method used was using light-dark bottles incubated for 4 hours. Samples were taken between 6-10, 10-14 and 14-18 hour, under the depth  0.2, 1.5, and 3 m. The results showed  that productivity in April – 2.4 to 2.17 ppm, May –0.6 to 2 ppm, and June –2.1 to 3.4 ppm. The regression correlation of the productivity to phytoplankton`s density is y = -0.03 + 0.01 x and r = 0.76, productivity to light intensity is y = -5.04 + 0.01 x and r = 0.63, productivity to water depth is y = 0.56 + 0.12x and r = 0.31,  productivity to turbidity is y = 1,78 + 0.33x and r = 0.35, productivity to water temperature is y = 1.91 – 0.08x and r = 0.32, productivity to pH is y = 1.39 – 0.71x and r = 0.20,  and productivity to CO2 content is y = 1.77 – 0.11 and r = 0.29. Based on the overall findings, the water quality condition was classified as eutrophic.
Keragaman Genetik Kultivar Pisang Diploid (AA) Koleksi Cibinong Science Center Berdasarkan Marka RAPD dan ISSR Poerba, Yuyu Suryasari; Ahmad, Fajarudin
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 15, No 3 (2010): October 2010
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (471.587 KB) | DOI: 10.24002/biota.v15i3.2584

Abstract

The banana (Musa acuminata Colla) is considered as an important crop plant due to its high economic value which also has good dietary source. Here, the genetic variation of 20 diploid (AA) banana cultivars from Cibinong Science Center collection were analyzed. Random amplified polymorphic DNAs (RAPDs) and Inter Simple Sequence Repeats fingerprinting of these banana cultivars were carried out by four primers of RPDSs and two primers of inter simple sequence repeats (ISSRs) led to DNA amplification. The amplification products of RPADs and ISSRs were polymorphic, 97.83% and 95%, respectively. Size of the bands was varied from 350bp to 2.0 kbp. The range of genetic distance was from 0.06 to 0.07. The molecular data showed that these banana varieties were diverse collection.
Phytochemicals As Bioactive Agents (Kajian Buku) Pranata, F. Sinung
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 8, No 2 (2003): June 2003
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (125.689 KB) | DOI: 10.24002/biota.v8i2.2891

Abstract

Pencegahan penyakit ternyata tidak terlepas dari diet beberapa makanan. Diet makanan yang kaya akan manfaat, seperti buah, sayur, dan biji-bijian telah diyakini berhubungan erat dengan pencegahan penyakit tertentu. Beberapa senyawa fitokimia yang terdapat dalam bahan makanan mempunyai aktivitas biologis dalam pencegahan penyakit, seperti kanker dan jantung. Buku PHYTOCHEMICALS AS BIOACTIVE AGENTS yang ditulis oleh Bidlack et al., ini menyuguhkan tentang aneka ragam senyawa organik yang dibentuk oleh tumbuhan, termasuk cara identifikasi dan evaluasi bioaktivitas fitokimia yang berperan dalam pencegahan penyakit tertentu.
Isolasi Bakteri Mananolitik dan Karakterisasi Mananasenya Meryandini, Anja; Anggreandari, Rizky; Rachmania, Nisa
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 13, No 2 (2008): June 2008
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (189.098 KB) | DOI: 10.24002/biota.v13i2.2675

Abstract

Isolate RA05 has the highest mannanolytic index and mannanase activity which isolated from copra soil waste from Pasaman, West Sumatra. The best growth condition that produces best mannanase activity of isolate RA05 was achieved from 500 ml flask containing 100 ml medium with 100 rpm agitation. Isolate RA05 showed its mannanase activity in medium containing Locust Bean Gum and coconut meal but not in medium containing kolang kaling. This mannanase had the highest activity on medium containing 2% of coconut meal with optimum condition temperatur 800C and pH 2.5. Adding of 5 mM MnCl2 on the crude enzym increased the activity near 300%. Other kation (Ca2+, Zn2+, Cu2+, Mg2+, Fe2+ dan Co2+) did not display great effect on the activity.
Aktivitas Antibakteri Ekstrak Metanol Buah Mbosi (Dysoxylum gaudichandianum (A.Juss) Miq.) dan Penapisan Senyawa Kimianya Praptiwi, Praptiwi; Harapini, Mindarti
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 10, No 2 (2005): June 2005
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (162.42 KB) | DOI: 10.24002/biota.v10i2.2844

Abstract

Mbosi (Dysoxylum gaudichandianum (A.Juss) Miq.) belongs to Meliaceae family, Its potential bioactivity had not been known although the other plants from the same family had been known as natural insectiside or medicinal plants. Phytochemical screening showed that mbosi fruit contained fat, fatty acid, sterol, triterpenoide, tannin, peroxide sugar, alkaloid, steroid glycoside and flavonoid. Identification of mbosi fruit chemical compounds by GC-MS showed that it contained 16 compounds. Compounds with the concentration more than 5 % were butyl cellosolve (11.1%), erythritol (8.48%), glycerol (10.32%), hexadecanioc acid palmitinic ester (16.2%), oleic acid (65.3%) and ethyl oleat (6.2%).  In-vitro antibacterial test on innoculated Mueller Hinton Agar to seven bacteria isolates (Salmonella typhimurium, S. typhii, Eschechiria coli ATCC 25923, Pseudomonas sp., Staphylococcus aureus ATCC 25922, S. epidermidis, and Bacilus subtilis)  showed that 20% methanol extract  inhibit only the growth of  Staphylococcus aureus.
Pseudo-Nitzschia pseudodelicatissima Isolated From Hurun Bay: Salinity Tolerance and Domoic Acid Content Panggabean, Lily
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 12, No 1 (2007): February 2007
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (383.763 KB) | DOI: 10.24002/biota.v12i1.2535

Abstract

Pseudo-nitzschia pseudodelicatissima dari sampel perairan Teluk Hurun, Lampung berhasil diisolasi dan dikultur dalam medium F/2 dengan penyinaran 2500 luks 12 jam per hari pada suhu 24-250C. Perlakuan salinitas menunjukkan bahwa P. pseudodelicatissima tidak toleran terhadap salinitas kurang dari 15 PSU dan sangat toleran terhadap salinitas yang tinggi hingga mencapai 45 PSU (Pressure Salinity Unit). Salinitas optimum untuk pertumbuhan P.pseudodelicatissima yaitu pada 30 dan 35 PSU. Analisis ekstrak P. pseudodelicatissima, 9 hari sesudah inokulasi, menggunakan HPLC-UV tidak menunjukkan adanya kandungan asam domoat.
Aktivitas Makan Monyet Ekor Panjang (Macaca fascicularis) di Bumi Perkemahan Pramuka, Cibubur, Jakarta Farida, Hilda; Perwitasari-Farajallah, Dyah; Tjitrosoedirdjo, Sri Sudarmiyati
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 15, No 1 (2010): February 2010
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (210.027 KB) | DOI: 10.24002/biota.v15i1.2642

Abstract

Long-tailed macaque (Macaca fascicularis) is one of the non human primates which is widely distributed. Long-tailed macaques live in a group consisted of multimales and multifemales. The group size was determined by predator, food resources, and foraging efficiency. Bumi Perkemahan Pramuka Cibubur is a habitat for long-tailed macaques. This place is a camp area and tourism site, which is predominated by akasia (Acacia auriculiformis). This research is aimed to study feeding activity of long-tailed macaque in Bumi Perkemahan Pramuka, Cibubur, Jakarta. Methods used in this research were habituation, census, ad libitum sampling, focal animal sampling, and scan sampling. The identification of food items was carried out by means of direct observation, interview, specimen collection, and identification of plant species. Feeding activities of long-tailed macaques constituted 9.01% of the total daily activities. Food consumed consists of natural and non natural food. Natural food items were leaf buds of awi tali (Gigantochloa apus) 34.76%, jukut pait (Axonopus compressus) 22.61%, and fig (Ficus benjamina) 7.15%, respectively. Furthermore, non natural food items were food leftovers of visitors 29.00% and peanuts 15.52%, respectively. In general, natural food (74.02%) was consumed more frequent than those of non natural food (25.98%).

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